Lactate Production in Isolated Swim Bladder Tissue of the European Eel Anguilla anguilla

1995 ◽  
Vol 68 (4) ◽  
pp. 634-646 ◽  
Author(s):  
Bernd Pelster
Keyword(s):  
Lactate Production ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Swim Bladder ◽  
Bladder Tissue

Swim bladder gas gland cells produce surfactant: in vivo and in culture

2000 ◽  
Vol 279 (6) ◽  
pp. R2336-R2343 ◽  
Author(s):  
C. Prem ◽  
W. Salvenmoser ◽  
J. Würtz ◽  
B. Pelster
Keyword(s):  
Surfactant Protein ◽  
Cross Reaction ◽  
Tissue Homogenate ◽  
European Eel ◽  
Microscopical Examination ◽  
Swim Bladder ◽  
Lamellar Bodies ◽  
Bladder Tissue ◽  
Gland Cells ◽  
Gas Gland

Electron microscopical examination of gas gland cells of the physostome European eel ( Anguilla anguilla) and of the physoclist perch ( Perca fluviatilis) revealed the presence of significant numbers of lamellar bodies, which are known to be involved in surfactant secretion. In the perch, in which the gas gland is a compact structure and gas gland cells are connected to the swim bladder lumen via small canals, lamellar bodies were also found in flattened cells forming the swim bladder epithelium. Flat epithelial cells are absent in the eel swim bladder, in which the whole epithelium consists of cuboidal gas gland cells. In both species, Western blot analysis using specific antibodies to human surfactant protein A (SP-A) showed a cross-reaction with swim bladder tissue homogenate proteins of ∼65 kDa and in the eel occasionally of ∼120 kDa, probably representing SP-A-like proteins in a dimeric and a tetrameric state. An additional band was observed at ∼45 kDa. Western blots using antibodies to rat SP-D again resulted in a single band at ∼45 kDa in both species, suggesting that there might be a cross-reaction of the antibody to human SP-A with an SP-D-like protein of the swim bladder tissue. To localize the surfactant protein, eel gas gland cells were cultured on permeable supports. Under these conditions, the gas gland cells regain their characteristic polarity. Electron microscopy confirmed the presence of lamellar bodies in cultured cells, and occasionally, exocytotic events were observed. Immunohistochemical staining using an antibody to human SP-A demonstrated the presence of surfactant protein only in luminal membranes and in adjacent lateral membranes. Only occasionally, evidence was found for the presence of surfactant protein in lamellar bodies.

scholarly journals GLUCOSE METABOLISM OF THE SWIMBLADDER TISSUE OF THE EUROPEAN EEL ANGUILLA ANGUILLA

1993 ◽  
Vol 185 (1) ◽  
pp. 169-178 ◽  
Author(s):  
B. Pelster ◽  
P. Scheid
Keyword(s):  
Glucose Uptake ◽  
Venous Blood ◽  
Lactate Concentration ◽  
Lactate Production ◽  
Anguilla Anguilla ◽  
Pentose Phosphate ◽  
Co2 Production ◽  
European Eel ◽  
O2 Uptake ◽  
Lactate Release

Glucose uptake from, and lactate release into, the blood have been analysed in the active gas- depositing swimbladder of the immobilized European eel Anguilla anguilla. Under normoxic conditions, 0.72 micromole min-1 glucose was removed from the blood supply, while lactate was released into it at a rate of 1.16 micromole min-1. The rate of gas deposition into the swimbladder was significantly correlated with the rate of lactate production. Under hypoxic conditions, glucose consumption by, and lactate production of, the swimbladder tissue were reduced, as was the rate of gas deposition. Compared with normoxic conditions, lactate concentration in the swimbladder tissue was elevated after 1 h of hypoxia, indicating a decrease in lactate release. No difference in the osmolality of arterial and venous blood could be detected in these experiments. Combining the data for glucose uptake and lactate release measured under normoxic conditions with the values for O2 uptake and CO2 production of the swimbladder tissue measured under similar conditions in a previous study, a quantitative evaluation of glucose catabolism was performed. According to the O2 uptake of the tissue, only about 1 % of the glucose was oxidized, while about 80 % was fermented to lactic acid. The remaining 0.14 micromole min-1 glucose was presumably catabolized through the pentose phosphate shunt, as indicated by the CO2 production of 0.16 micromole min-1 that cannot be explained by aerobic metabolism.

Introduced parasite Anguillicola crassus infection significantly impedes swim bladder function in the European eel Anguilla anguilla (L.)

2014 ◽  
Vol 37 (10) ◽  
pp. 921-924 ◽  
Author(s):  
J Barry ◽  
J McLeish ◽  
J A Dodd ◽  
J F Turnbull ◽  
P Boylan ◽  
...  
Keyword(s):  
Anguilla Anguilla ◽  
Bladder Function ◽  
European Eel ◽  
Swim Bladder ◽  
Anguillicola Crassus

Regulation ofAnguillicola crassus(Nematoda) infrapopulations in their definitive host, the European eel,Anguilla anguilla

Parasitology ◽  
2008 ◽  
Vol 135 (14) ◽  
pp. 1707-1716 ◽  
Author(s):  
G. FAZIO ◽  
P. SASAL ◽  
C. DA SILVA ◽  
B. FUMET ◽  
J. BOISSIER ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Anguilla Anguilla ◽  
The Body ◽  
European Eel ◽  
Swim Bladder ◽  
Experimental Conditions ◽  
Larval Stages ◽  
Resource Limited ◽  
Third Stage ◽  
Opposite Sex

SUMMARYThe parasitic nematodeAnguillicola crassuswas recently introduced into populations of the European eel,Anguilla anguilla. We investigated, under experimental conditions, the regulation ofA. crassusinfrapopulations. We tested the effects of (1) the resource-limited habitat of the parasite and (2) the coexistence of several developmental stages in its niche (the swim-bladder) on the composition of the infrapopulations. The results revealed that the respective effects of these factors differed substantially during the course of the infection. Third-stage larvae (L3s) establishment would not be constrained by the size of the swim-bladder. Their moult to fourth-stage larvae (L4s) would be accelerated as the number of L3s increased. The moulting time of L4s to adults would be reduced by males and would be constrained by the size of the swim-bladder. However, the moult of L4s to adults and their further development would be synchronized with those of the opposite sex. At the time of mating, the number of males and the body weight of adults would depend on the size of the swim-bladder. Soon after the laying of eggs, the developmental constraint on the late L3s would decrease. When adults die, constraints would cease and late larval stages would moult to become adults.

Proteomic Studies on the Swim Bladder of the European Eel (Anguilla anguilla )

PROTEOMICS ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 1700445 ◽  
Author(s):  
Fernando J. Sialana ◽  
Gabriel Schneebauer ◽  
Ana Paunkov ◽  
Bernd Pelster ◽  
Gert Lubec
Keyword(s):  
Anguilla Anguilla ◽  
European Eel ◽  
Swim Bladder

scholarly journals Metabolism of the Perfused Swtmbladder of the European EEL: Oxygen, Carbon Dioxide, Glucose and Lactate Balance

1989 ◽  
Vol 144 (1) ◽  
pp. 495-506 ◽  
Author(s):  
B. PELSTER ◽  
H. KOBAYASHI ◽  
P. SCHEID
Keyword(s):  
Lactate Production ◽  
Anguilla Anguilla ◽  
Co2 Production ◽  
European Eel ◽  
Fick Principle ◽  
Partial Pressures ◽  
Gland Tissue ◽  
Arterial Inflow ◽  
Co2 Washout ◽  
Gas Gland

We have measured the metabolic activity in the vascularly isolated, salineperfused swimbladder of the eel (Anguilla anguilla) in order to investigate the pathways for CO2 formation in the gas gland tissue. Concentrations of O2, CO2, glucose and lactate were measured in the arterial inflow and venous outflow of the swimbladder, and metabolic rates were calculated by the direct Fick principle. 1. Total CO2 production, averaging 55.8nmol min−1, was about 4.6 times the O2 consumption (mean 12.0nmol min−1). This suggests that only about 22% of the CO2 is formed by aerobic glucose metabolism. 2. CO2 formation from HCO3− or CO2 washout does not appear to be significant in our experiments with steady perfusion of a saline containing a low level of HCO3−. 3. The ratio of lactate production to glucose uptake averaged 1.2, indicating that only 60% of the glucose is converted to lactate. Since only 1–2% of the glucose was found to be oxidized (2 nmol min−1), the extra glucose appears to be anoxidatively metabolized to CO2. 4. The anoxidative CO2 formation appears to be of functional importance for producing the high gas partial pressures of both CO2 and O2 which are required for secretion of these gases into the swimbladder.

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