Identification of traction-separation parameters by means of peel testing and in situ confocal microscopy

We explored the relationship in chick embryos between somitogenesis and the onset of somite myogenesis by immunodetection of the muscle-specific intermediate filament protein desmin. Early somite desmin expression was detected by whole-mount in situ confocal microscopy. No detectable somite desmin was observed in embryos of 15 somites (Stage 12) or younger. In embryos having between 16 and 26 somites (Stages 12-15), desmin could be detected in somites positioned increasingly more caudal in the embryo. Finally, in embryos of 27 somites (Stage 16) and older, somite desmin expression was consistently present in all but the caudal-most six somites. Although the rate of somite formation is fairly constant, the rate of observed somite desmin expression progressing caudally in the embryo is greater initially than the rate of segmentation. After an embryo has formed about 27 somites, the rate of desmin appearance parallels the rate of segmentation at a distance of about six somites. This result suggests that very early somite myogenesis is not linked to somitogenesis.

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The use of fluorescent in situ hybridization for the analysis of nuclear architecture by confocal microscopy

Cell Biology International Reports ◽

10.1016/s0309-1651(05)80018-9 ◽

1992 ◽

Vol 16 (8) ◽

pp. 739-747 ◽

Cited By ~ 8

Author(s):

R HULSPAS ◽

J BAUMAN

Keyword(s):

In Situ Hybridization ◽

Confocal Microscopy ◽

Fluorescent In Situ Hybridization ◽

Nuclear Architecture

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3d Confocal Microscopy and Image Analysis for Measurement of Genetic Instability

Microscopy and Microanalysis ◽

10.1017/s1431927600018432 ◽

1999 ◽

Vol 5 (S2) ◽

pp. 1022-1023

Author(s):

C. Ortiz de Solorzano ◽

K. Chin ◽

D. Knowles ◽

A. Jones ◽

E. Garcia ◽

...

Keyword(s):

Image Analysis ◽

Confocal Microscopy ◽

Genetic Instability ◽

Copy Number ◽

3D Image ◽

3D Images ◽

Tissue Sections ◽

Total Dna ◽

Natural Tissue

Solid tumors frequently contain cells that are heterogeneous in the copy number of DNA loci. This fact implies the existence of genetic instability, which may be associated with disease aggressiveness. Accurate measurement of this phenomenon requires analysis of intact nuclei within their natural tissue context. We perform these measurements by preparing >30μm thick tissue sections, labeling them with fluorescent labels for total DNA and for specific DNA loci using fluorescence in situ hybridization (FISH) which retain the transparency of the tissue and acquiring 3D images of the tissue using confocal microscopy (figure 1). In this study, we combined automated 3D image analysis (IA) algorithms for segmenting individual nuclei based on the total DNA stain1 and for segmenting the punctuate FISH signals of DNA loci. This enables us to efficiently enumerate the copy number of specific DNA loci in individual cells and as a function of the cell's location in the tissue.

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In-situ disintegration of egg white gels by pepsin and kinetics of nutrient release followed by time-lapse confocal microscopy

Food Hydrocolloids ◽

10.1016/j.foodhyd.2019.105228 ◽

2020 ◽

Vol 98 ◽

pp. 105228 ◽

Cited By ~ 4

Author(s):

Geeshani Somaratne ◽

Francoise Nau ◽

Maria J. Ferrua ◽

Jaspreet Singh ◽

Aiqian Ye ◽

...

Keyword(s):

Confocal Microscopy ◽

Nutrient Release ◽

Time Lapse ◽

Egg White ◽

Kinetics Of

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Detecting Early Apoptosis in Whole Epithelial Sheets with a Caspase 3 Substrate, Phiphilux and Annexes V Using Confocal Microscopy

Microscopy and Microanalysis ◽

10.1017/s143192760002907x ◽

2001 ◽

Vol 7 (S2) ◽

pp. 600-601

Author(s):

Kathy K. H. Svoboda

Keyword(s):

In Situ Hybridization ◽

Confocal Microscopy ◽

General Principle ◽

Caspase 3 ◽

Living Cells ◽

Corneal Epithelial ◽

Early Apoptosis ◽

Epithelial Sheets ◽

Conventional Light Microscope

Many reagents have been developed recently to label living cells with substrates that will become fluorescent if an enzyme is active. The general principle is that the substrate will be taken up by living cells then detected only if the enzyme is active. These substrates work well with isolated individual cells, however, more difficulty can be encountered when studying whole tissues. Problems can range from substrate penetration into whole tissues to being able to detect the label effectively. We have used the chicken corneal epithelia for many studies, but the tissue is to thick to view with a conventional light microscope, therefore we have developed techniques using laser confocal microscopes to view this tissue in with a variety of techniques including in situ hybridization, immunohistochemistry, and vital dyes/stains.Whole embryonic corneal epithelial sheets can be isolated without the basal lamina.

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