Facile multifunctional-mode of fabricated biocompatible human serum albumin/ reduced graphene oxide/Cladophora glomerata nanoparticles for bacteriostatic phototherapy, bacterial tracking and antioxidant potential

Multifunctional human serum albumin-modified reduced graphene oxide can specifically target HCC cells and effectively kill them with the help of a NIR laser.

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Development of a Novel ssDNA Sequence for a Glycated Human Serum Albumin and Construction of a Simple Aptasensor System Based on Reduced Graphene Oxide (rGO)

Biosensors ◽

10.3390/bios10100141 ◽

2020 ◽

Vol 10 (10) ◽

pp. 141

Author(s):

A-Ru Kim ◽

Yeongmi Choi ◽

Sang-Heon Kim ◽

Hyun-Seok Moon ◽

Jae-Ho Ko ◽

...

Keyword(s):

Graphene Oxide ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Reduced Graphene Oxide ◽

Limit Of Detection ◽

Fluorescence Signal ◽

Quenching Effect ◽

High Affinity ◽

Reduced Graphene

Diabetes is one of the top 10 global causes of death. About one in 11 global adults have diabetes. As the disease progresses, the mortality rate increases, and complications can develop. Thus, early detection and effective management of diabetes are especially important. Herein, we present a novel glycated human serum albumin (GHSA) aptamer, i.e., GABAS-01, which has high affinity and specificity. The aptamer was selected by reduced graphene oxide-based systematic evolution of ligands by exponential enrichement (rGO-based SELEX) against GHSA. After five rounds of selection through gradually harsher conditions, GABAS-01 with high affinity and specificity for the target was obtained. GABAS-01 was labeled by FAM at the 5′-end and characterized by measuring the recovery of a fluorescence signal that is the result of fluorescence quenching effect of rGO. As a result, GABAS-01 had low-nanomolar Kd values of 1.748 ± 0.227 nM and showed a low limit of detection of 16.40 μg/mL against GHSA. This result shows the potential application of GABAS-01 as an effective on-site detection probe of GHSA. In addition, these properties of GABAS-01 are expected to contribute to detection of GHSA in diagnostic fields.

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Mechanistic insight into the binding of graphene oxide with human serum albumin: Multispectroscopic and molecular docking approach

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy ◽

10.1016/j.saa.2021.119750 ◽

2021 ◽

pp. 119750

Author(s):

Afroz Khan ◽

Fauzia Khan ◽

Moyad Shahwan ◽

Mohd Shahnawaz Khan ◽

Fohad Mabood Husain ◽

...

Keyword(s):

Graphene Oxide ◽

Molecular Docking ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Mechanistic Insight ◽

Docking Approach ◽

Insight Into

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Reduced graphene oxide nanosheets for selective picomolar detection of Bovine serum albumin

IEEE Transactions on NanoBioscience ◽

10.1109/tnb.2021.3118726 ◽

2021 ◽

pp. 1-1

Author(s):

Kunal Biswas ◽

Avik Sett ◽

Monojit Mondal ◽

Srijeet Tripathy ◽

Jaya Bandyopadhyay ◽

...

Keyword(s):

Graphene Oxide ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Reduced Graphene Oxide ◽

Bovine Serum ◽

Graphene Oxide Nanosheets ◽

Reduced Graphene

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An Electrochemically Reduced Copper/Reduced Graphene Oxide Film Modified Electrode for Sensitive Non-Enzymatic Glucose Detection in Human Serum

10.21203/rs.3.rs-264254/v1 ◽

2021 ◽

Author(s):

Sopit Phetsang ◽

Pinit Kidkhunthod ◽

Narong Chanlek ◽

Jaroon Jakmunee ◽

Pitchaya Mungkornasawakul ◽

...

Keyword(s):

Graphene Oxide ◽

Human Serum ◽

Reduced Graphene Oxide ◽

Modified Electrode ◽

Electrocatalytic Activity ◽

Glucose Sensor ◽

Electrochemical Sensors ◽

High Sensitivity ◽

Detection Range ◽

Reduced Graphene

Abstract Numerous studies suggest that modification with functional nanomaterials can enhance the electrode electrocatalytic activity, sensitivity, and selectivity of the electrochemical sensors. Here, a highly sensitive and cost-effective disposable non-enzymatic glucose sensor based on copper(II)/reduced graphene oxide modified screen-printed carbon electrode is demonstrated. Facile fabrication of the developed sensing electrodes is carried out by the adsorption of copper(II) onto graphene oxide modified electrode, then following the electrochemical reduction. The proposed sensor illustrates good electrocatalytic activity toward glucose oxidation with a wide linear detection range from 0.10 mM to 12.5 mM, low detection limit of 65 µM, and high sensitivity of 172 µA mM− 1 cm− 2 along with satisfactory anti-interference ability, reproducibility, stability, and the acceptable recoveries for the detection of glucose in a human serum sample (95.6–106.4%). The copper(II)/reduced graphene oxide based sensor with the superior performances is a great potential for the quantitation of glucose in real samples.

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