scholarly journals Development of a Novel ssDNA Sequence for a Glycated Human Serum Albumin and Construction of a Simple Aptasensor System Based on Reduced Graphene Oxide (rGO)

Biosensors ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 141
Author(s):  
A-Ru Kim ◽  
Yeongmi Choi ◽  
Sang-Heon Kim ◽  
Hyun-Seok Moon ◽  
Jae-Ho Ko ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Fluorescence Signal ◽  
Quenching Effect ◽  
High Affinity ◽  
Reduced Graphene

Diabetes is one of the top 10 global causes of death. About one in 11 global adults have diabetes. As the disease progresses, the mortality rate increases, and complications can develop. Thus, early detection and effective management of diabetes are especially important. Herein, we present a novel glycated human serum albumin (GHSA) aptamer, i.e., GABAS-01, which has high affinity and specificity. The aptamer was selected by reduced graphene oxide-based systematic evolution of ligands by exponential enrichement (rGO-based SELEX) against GHSA. After five rounds of selection through gradually harsher conditions, GABAS-01 with high affinity and specificity for the target was obtained. GABAS-01 was labeled by FAM at the 5′-end and characterized by measuring the recovery of a fluorescence signal that is the result of fluorescence quenching effect of rGO. As a result, GABAS-01 had low-nanomolar Kd values of 1.748 ± 0.227 nM and showed a low limit of detection of 16.40 μg/mL against GHSA. This result shows the potential application of GABAS-01 as an effective on-site detection probe of GHSA. In addition, these properties of GABAS-01 are expected to contribute to detection of GHSA in diagnostic fields.

Multifunctional human serum albumin-modified reduced graphene oxide for targeted photothermal therapy of hepatocellular carcinoma

RSC Advances ◽  
2016 ◽  
Vol 6 (14) ◽  
pp. 11167-11175 ◽  
Author(s):  
Aixian Zheng ◽  
Da Zhang ◽  
Ming Wu ◽  
Huanghao Yang ◽  
Xiaolong Liu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Graphene Oxide ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Reduced Graphene Oxide ◽  
Photothermal Therapy ◽  
Reduced Graphene ◽  
Nir Laser ◽  
Hcc Cells

Multifunctional human serum albumin-modified reduced graphene oxide can specifically target HCC cells and effectively kill them with the help of a NIR laser.

scholarly journals Reduced Graphene Oxide Fibre Electrodes for Drug Sensing

Proceedings ◽  
2021 ◽  
Vol 68 (1) ◽  
pp. 18
Author(s):  
Sutthima Sriprasertsuk ◽  
John R. Varcoe ◽  
Carol Crean
Keyword(s):  
Cyclic Voltammetry ◽  
Graphene Oxide ◽  
Carbon Fibre ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Linear Range ◽  
Oxidation Peak ◽  
Reduced Graphene ◽  
Fibre Sensor ◽  
Model Drug

Reduced graphene oxide (rGO) fibre electrodes and their ability to sense paracetamol (as model drug) were studied. rGO was electrodeposited onto carbon fibre by two different approaches: potentiostatic deposition and cyclic voltammetry (CV) in the presence of graphene oxide solution. Carbon fibre electrodes coated with rGO (after five CV cycles) could sense paracetamol with an oxidation peak at 0.62 V (vs. Ag/AgCl). The limit of detection of this fibre sensor was found to be 36.3 µM with a linear range of 50–500 µM of paracetamol (R2 = 0.9901).

Reduced graphene oxide nanosheets for selective picomolar detection of Bovine serum albumin

2021 ◽  
pp. 1-1
Author(s):  
Kunal Biswas ◽  
Avik Sett ◽  
Monojit Mondal ◽  
Srijeet Tripathy ◽  
Jaya Bandyopadhyay ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Reduced Graphene Oxide ◽  
Bovine Serum ◽  
Graphene Oxide Nanosheets ◽  
Reduced Graphene

An Electrochemically Reduced Copper/Reduced Graphene Oxide Film Modified Electrode for Sensitive Non-Enzymatic Glucose Detection in Human Serum

2021 ◽  
Author(s):  
Sopit Phetsang ◽  
Pinit Kidkhunthod ◽  
Narong Chanlek ◽  
Jaroon Jakmunee ◽  
Pitchaya Mungkornasawakul ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Human Serum ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Electrocatalytic Activity ◽  
Glucose Sensor ◽  
Electrochemical Sensors ◽  
High Sensitivity ◽  
Detection Range ◽  
Reduced Graphene

Abstract Numerous studies suggest that modification with functional nanomaterials can enhance the electrode electrocatalytic activity, sensitivity, and selectivity of the electrochemical sensors. Here, a highly sensitive and cost-effective disposable non-enzymatic glucose sensor based on copper(II)/reduced graphene oxide modified screen-printed carbon electrode is demonstrated. Facile fabrication of the developed sensing electrodes is carried out by the adsorption of copper(II) onto graphene oxide modified electrode, then following the electrochemical reduction. The proposed sensor illustrates good electrocatalytic activity toward glucose oxidation with a wide linear detection range from 0.10 mM to 12.5 mM, low detection limit of 65 µM, and high sensitivity of 172 µA mM− 1 cm− 2 along with satisfactory anti-interference ability, reproducibility, stability, and the acceptable recoveries for the detection of glucose in a human serum sample (95.6–106.4%). The copper(II)/reduced graphene oxide based sensor with the superior performances is a great potential for the quantitation of glucose in real samples.

Reduced Graphene Oxide Supported Molybdenum Oxide Hybrid Nanocomposites: High Performance Electrode Material for Supercapacitor and Photocatalytic Applications

2020 ◽  
Vol 20 (7) ◽  
pp. 4035-4046
Author(s):  
Rengasamy Dhanabal ◽  
Dhanasekaran Naveena ◽  
Sivan Velmathi ◽  
Arumugam Chandra Bose
Keyword(s):  
Electron Microscopy ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Photoelectron Spectroscopy ◽  
Diffuse Reflectance Spectroscopy ◽  
Hybrid Nanocomposites ◽  
X Ray ◽  
Supercapacitor Application ◽  
Quenching Effect ◽  
Reduced Graphene

Using a simple solution based synthesis route, hexagonal MoO3 (h-MoO3) nanorods on reduced graphene oxide (RGO) sheets were prepared. The structure and morphology of resulting RGO-MoO3 nanocomposite were characterized using X-ray diffraction (XRD), Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), transmission electron microscopy (TEM) and field emission scanning electron microscopy (FESEM). The optical property was studied using UV-Visible diffuse reflectance spectroscopy (UV-Vis DRS) and photoluminescence spectroscopy (PL). The RGO-MoO3 nanocomposites were used as an electrode for supercapacitor application and photocatalyst for photodegradation of methylene blue (MB) and rhodamine B (RhB) under visible light irradiation. We demonstrated that the RGO-MoO3 electrode is capable of delivering high specific capacitance of 134 F/g at current density of 1 A/g with outstanding cyclic stability for 2000 cycles. The RGOMoO3 photocatalyst degrades 95% of MB dye within 90 min, and a considerable recyclability up to 4 cycles was observed. The quenching effect of scavengers test confirms holes are main reactive species in the photocatalytic degradation of MB. Further, the charge transfer process between RGO and MoO3 was schematically demonstrated.

scholarly journals Biological characteristics of two lysines on human serum albumin in the high-affinity binding of 4Z,15Z-bilirubin-IXα revealed by phage display

FEBS Journal ◽  
2011 ◽  
Vol 278 (21) ◽  
pp. 4100-4111 ◽  
Author(s):  
Ai Minomo ◽  
Yu Ishima ◽  
Ulrich Kragh-Hansen ◽  
Victor T. G. Chuang ◽  
Makiyo Uchida ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Phage Display ◽  
Human Serum ◽  
Biological Characteristics ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity

scholarly journals A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers

2020 ◽  
Vol 187 (5) ◽  
Author(s):  
Jagriti Sethi ◽  
Michiel Van Bulck ◽  
Ahmed Suhail ◽  
Mina Safarzadeh ◽  
Ana Perez-Castillo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Label Free ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

AbstractA label-free biosensor is developed for the determination of plasma-based Aβ1–42 biomarker in Alzheimer’s disease (AD). The platform is based on highly conductive dual-layer of graphene and electrochemically reduced graphene oxide (rGO). The modification of dual-layer with 1-pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) is achieved to facilitate immobilization of H31L21 antibody. The effect of these modifications were studied with morphological, spectral and electrochemical techniques. The response of the biosensor was evaluated using differential pulse voltammetry (DPV). The data was acquired at a working potential of ~ 180 mV and a scan rate of 50 mV s−1. A low limit of detection (LOD) of 2.398 pM is achieved over a wide linear range from 11 pM to 55 nM. The biosensor exhibits excellent specificity over Aβ1–40 and ApoE ε4 interfering species. Thus, it provides a viable tool for electrochemical determination of Aβ1–42. Spiked human and mice plasmas were used for the successful validation of the sensing platform in bio-fluidic samples. The results obtained from mice plasma analysis concurred with the immunohistochemistry (IHC) and magnetic resonance imaging (MRI) data obtained from brain analysis.

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