Interference-free SERS nanoprobes for labeling and imaging of MT1-MMP in breast cancer cells

2021 ◽  
Author(s):  
Dan Zhu ◽  
Anran Li ◽  
Yunsong Di ◽  
Zhuyuan Wang ◽  
Jingzhan Shi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Clinical Settings ◽  
Free Peptide ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Targeting Peptide ◽  
Membrane Type

Abstract The expression of membrane type-1 matrix metalloproteinase (MT1-MMP) in cancer cells is critical for understanding the development, invasion and metastasis of cancers. In this study, we devised an interference-free surface-enhanced Raman scattering (SERS) nanoprobe with high selectivity and specificity for MT1-MMP. The nanoprobe was comprised of silver core-silica shell nanoparticle with a Raman reporter tag (4-mercaptobenzonitrile) embedded in the interface. Moreover, the nitrile group in 4-mercaptobenzonitrile shows a unique characteristic peak in the Raman-silent region (1800-2800 cm-1), which eliminates spectral overlapping or background interference in the Raman fingerprint region (500-1800 cm-1). After surface modification with a targeting peptide, the nanoprobe allowed visualization and evaluation of MT1-MMP in breast cancer cells via SERS spectrometry. This interference-free, peptide functionalized SERS nanoprobe is supposed to be conducive to early diagnosis and invasive assessment of cancer in clinical settings.

Surface-Enhanced Raman Scattering Optophysiology Nanofibers for the Detection of Heavy Metals in Single Breast Cancer Cells

ACS Sensors ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 1649-1662
Author(s):  
Xingjuan Zhao ◽  
Shirley Campbell ◽  
Patrick Z. El-Khoury ◽  
Yuechen Jia ◽  
Gregory Q. Wallace ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Heavy Metals ◽  
Raman Scattering ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

scholarly journals Development of a Nanostructured Platform for Identifying HER2-Heterogeneity of Breast Cancer Cells by Surface-Enhanced Raman Scattering

Nanomaterials ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 549 ◽  
Author(s):  
Alexandro Téllez-Plancarte ◽  
Emmanuel Haro-Poniatowski ◽  
Michel Picquart ◽  
José Morales-Méndez ◽  
Carlos Lara-Cruz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Raman Scattering ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Surface Enhanced Raman Scattering ◽  
Laser Scanning Microscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Mcf 7

Biosensor technology has great potential for the detection of cancer through tumor-associated molecular biomarkers. In this work, we describe the immobilization of the recombinant humanized anti-HER2 monoclonal antibody (trastuzumab) on a silver nanostructured plate made by pulsed laser deposition (PLD), over a thin film of Au(111). Immobilization was performed via 4-mercapto benzoic acid self-assembled monolayers (4-MBA SAMs) that were activated with coupling reagents. A combination of immunofluorescence images and z-stack analysis by confocal laser scanning microscopy (CLSM) allowed us to detect HER2 presence and distribution in the cell membranes. Four different HER2-expressing breast cancer cell lines (SKBR3 +++, MCF-7 +/−, T47D +/−, MDA-MB-231 −) were incubated during 24 h on functionalized silver nanostructured plates (FSNP) and also on Au(111) thin films. The cells were fixed by means of an ethanol dehydration train, then characterized by atomic force microscopy (AFM) and surface-enhanced Raman scattering (SERS). SERS results showed the same tendency as CLSM findings (SKBR3 > MCF-7 > T47D > MDA-MB-231), especially when the Raman peak associated with phenylalanine amino acid (1002 cm−1) was monitored. Given the high selectivity and high sensitivity of SERS with a functionalized silver nanostructured plate (FSNP), we propose this method for identifying the presence of HER2 and consequently, of breast cancer cells.

Distinguishing breast cancer cells using surface-enhanced Raman scattering

2011 ◽  
Vol 402 (3) ◽  
pp. 1093-1100 ◽  
Author(s):  
Jing Yang ◽  
Zhuyuan Wang ◽  
Shenfei Zong ◽  
Chunyuan Song ◽  
Ruohu Zhang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Raman Scattering ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

scholarly journals Surface enhanced Raman spectroscopy in breast cancer cells

LASER THERAPY ◽  
2013 ◽  
Vol 22 (1) ◽  
pp. 37-42 ◽  
Author(s):  
JL Gonz^|^aacute;lez-Sol^|^iacute;s ◽  
GH Lu^|^eacute;vano-Colmenero ◽  
J Vargas-Mancilla
Keyword(s):  
Breast Cancer ◽  
Raman Spectroscopy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Simultaneous isolation and detection of single breast cancer cells using surface-enhanced Raman spectroscopy

Talanta ◽  
2018 ◽  
Vol 186 ◽  
pp. 44-52 ◽  
Author(s):  
Melina Yarbakht ◽  
Maryam Nikkhah ◽  
Ahmad Moshaii ◽  
Karina Weber ◽  
Christian Matthäus ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Raman Spectroscopy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Controllable Drug Delivery by Na+/K+ ATPase α1 Targeting Peptide Conjugated DSPE-PEG Nanocarriers for Breast Cancer

2021 ◽  
Vol 20 ◽  
pp. 153303382110278
Author(s):  
Yayan Yang ◽  
Qian Feng ◽  
Chuanfeng Ding ◽  
Wei Kang ◽  
Xiufeng Xiao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Click Reaction ◽  
Chemotherapy Drugs ◽  
Targeting Peptide ◽  
And Migration

Although Epirubicin (EPI) is a commonly used anthracycline for the treatment of breast cancer in clinic, the serious side effects limit its long-term administration including myelosuppression and cardiomyopathy. Nanomedicines have been widely utilized as drug delivery vehicles to achieve precise targeting of breast cancer cells. Herein, we prepared a DSPE-PEG nanocarrier conjugated a peptide, which targeted the breast cancer overexpression protein Na+/K+ ATPase α1 (NKA-α1). The nanocarrier encapsulated the EPI and grafted with the NKA-α1 targeting peptide through the click reaction between maleimide and thiol groups. The EPI was slowly released from the nanocarrier after entering the breast cancer cells with the guidance of the targeting NKA-α1 peptide. The precise and controllable delivery and release of the EPI into the breast cancer cells dramatically inhibited the cells proliferation and migration in vitro and suppressed the tumor volume in vivo. These results demonstrate significant prospects for this nanocarrier as a promising platform for numerous chemotherapy drugs.

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