Poly(butyl cyanoacrylate) nanoparticles-delivered β-nerve growth factor promotes the neurite outgrowth and reduces the mortality in the rat after traumatic brain injury

2021 ◽  
Author(s):  
Yu Wang ◽  
Feng Jia ◽  
Yong Lin
Keyword(s):  
Traumatic Brain Injury ◽  
Nerve Growth Factor ◽  
Brain Injury ◽  
Growth Factor ◽  
Neurite Outgrowth ◽  
Tween 80 ◽  
Nerve Growth ◽  
Neurotrophic Activity ◽  
Molecular Therapeutic

Abstract Several transport vectors, including nanoparticles, have been reported to be used for the delivery of therapeutic medicines crossing the impermeable blood-brain barrier (BBB) to treat the diseases in the central nerve system (CNS), such as traumatic brain injury (TBI). Poly(n-butyl-2-cyanoacrylate) (PBCA) nanoparticles, made from biocompatible material, are regarded as a better potential delivery tool than others such as gold nanoparticles due to their degradability in vivo. However, little is known whether PBCA nanoparticles can be used to deliver neurotrophic factors into the brain to treat TBI. In this study, we first synthesized PBCA-carried β-nerve growth factor, a neurotrophic agent with a large molecular weight, and then intravenously injected the compound into TBI rats. We found that despite undergoing several synthesis steps and host circulation, β-NGF was able to be successfully delivered into the injured brain by PBCA nanoparticles, still maintain its neurotrophic activity for neurite outgrowth, and could reduce the mortality of TBI rats. Our findings indicate that PBCA nanoparticles, with Tween 80, are an efficient delivery vector and a protective reservoir for large molecular therapeutic agents to treat TBI intravenously.

Hypothermia Attenuates the Normal Increase in Interleukin 1β RNA and Nerve Growth Factor Following Traumatic Brain Injury in the Rat

1995 ◽  
Vol 12 (2) ◽  
pp. 159-167 ◽  
Author(s):  
JAMES R. GOSS ◽  
SCOT D. STYREN ◽  
PETER D. MILLER ◽  
PATRICK M. KOCHANEK ◽  
ALAN M. PALMER ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Nerve Growth Factor ◽  
Brain Injury ◽  
Growth Factor ◽  
Interleukin 1Β ◽  
Nerve Growth ◽  
Normal Increase

scholarly journals Nerve growth factor expression correlates with severity and outcome of traumatic brain injury in children

2008 ◽  
Vol 12 (3) ◽  
pp. 195-204 ◽  
Author(s):  
Antonio Chiaretti ◽  
Alessia Antonelli ◽  
Riccardo Riccardi ◽  
Orazio Genovese ◽  
Patrizio Pezzotti ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Nerve Growth Factor ◽  
Brain Injury ◽  
Growth Factor ◽  
Nerve Growth ◽  
Nerve Growth Factor Expression ◽  
Factor Expression ◽  
Growth Factor Expression

Interleukin-6 and Nerve Growth Factor Upregulation Correlates with Improved Outcome in Children with Severe Traumatic Brain Injury

2008 ◽  
Vol 25 (3) ◽  
pp. 225-234 ◽  
Author(s):  
Antonio Chiaretti ◽  
Alessia Antonelli ◽  
Antonio Mastrangelo ◽  
Patrizio Pezzotti ◽  
Luca Tortorolo ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Nerve Growth Factor ◽  
Brain Injury ◽  
Growth Factor ◽  
Severe Traumatic Brain Injury ◽  
Interleukin 6 ◽  
Nerve Growth ◽  
Improved Outcome

Human β nerve growth factor obtained from a baculovirus expression system has potent in vitro and in vivo neurotrophic activity

1990 ◽  
Vol 110 (1) ◽  
pp. 11-24 ◽  
Author(s):  
Jim Barnett ◽  
Preston Baecker ◽  
Carol Routledge-Ward ◽  
Hela Bursztyn-Pettegrew ◽  
Joan Chow ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Nerve Growth ◽  
Neurotrophic Activity ◽  
Baculovirus Expression

Nerve Growth Factor Attenuates Cholinergic Deficits Following Traumatic Brain Injury in Rats

1997 ◽  
Vol 146 (2) ◽  
pp. 479-490 ◽  
Author(s):  
C.Edward Dixon ◽  
Paul Flinn ◽  
Juliang Bao ◽  
Richard Venya ◽  
Ronald L. Hayes
Keyword(s):  
Traumatic Brain Injury ◽  
Nerve Growth Factor ◽  
Brain Injury ◽  
Growth Factor ◽  
Nerve Growth

Astrocytes Are the Major Source of Nerve Growth Factor Upregulation Following Traumatic Brain Injury in the Rat

1998 ◽  
Vol 149 (2) ◽  
pp. 301-309 ◽  
Author(s):  
James R. Goss ◽  
Mark E. O'Malley ◽  
Lanling Zou ◽  
Scot D. Styren ◽  
Patrick M. Kochanek ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Nerve Growth Factor ◽  
Brain Injury ◽  
Growth Factor ◽  
Nerve Growth

Intranasal nerve growth factor attenuates tau phosphorylation in brain after traumatic brain injury in rats

2014 ◽  
Vol 345 (1-2) ◽  
pp. 48-55 ◽  
Author(s):  
Qiushi Lv ◽  
Wenya Lan ◽  
Wenshan Sun ◽  
Ruidong Ye ◽  
Xiaobing Fan ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Nerve Growth Factor ◽  
Brain Injury ◽  
Growth Factor ◽  
Tau Phosphorylation ◽  
Nerve Growth

scholarly journals Regulation of microtubule composition and stability during nerve growth factor-promoted neurite outgrowth.

1986 ◽  
Vol 103 (2) ◽  
pp. 545-557 ◽  
Author(s):  
M M Black ◽  
J M Aletta ◽  
L A Greene
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Time Course ◽  
Peptide Mapping ◽  
Cell Protein ◽  
Nerve Growth ◽  
Associated Proteins

We have used the nerve growth factor (NGF)-responsive line of PC12 pheochromocytoma cells as a model system to study microtubule specializations associated with neurite outgrowth. PC12 cells treated with NGF cease proliferating and extend neurites. Long-term NGF treatment results in a two- to threefold increase in the proportion of total cellular tubulin that is polymerized in PC12 cells. The increase in this parameter first becomes apparent at 2-4 d with NGF and increases steadily thereafter. Several changes in microtubule-associated proteins (MAPs) of PC12 cells also occur after exposure to NGF. In immunoprecipitation assays, we observed the levels of MAP-2 to increase by at least several-fold after treatment with NGF. We also found that the compositions of three MAP classes with apparent Mr of 64K, 67K, and 80K are altered by NGF treatment. These MAPs, recently designated "chartins," are biochemically and immunologically distinct from the similarly-sized tau MAPs (Peng et al., 1985 Brain Res. 361: 200; Magendantz and Solomon, 1985 Proc. Natl. Acad. Sci. 82: 6581). In two-dimensional isoelectric focusing x SDS polyacrylamide gels, each chartin MAP class resolves into a set of proteins of similar apparent Mr but distinct pI. Peptide mapping analyses confirm that the isoelectric variants comprising each chartin MAP class are closely related in primary structure. Several striking differences in the composition of the chartin MAPs of PC12 cells grown with or without NGF were consistently observed. In particular, following longterm NGF treatment, the abundances of the more acidic variants of each chartin MAP class were markedly enhanced relative to the more basic members. This occurs without substantial changes in the abundance of each MAP class as a whole relative to total cell protein. The combined results of in vivo phosphorylation and peptide mapping experiments indicate that the NGF-inducible chartin MAP species are not primary translation products, but are generated posttranslationally, apparently by differential phosphorylation of other chartin MAPs. These observations suggest that NGF treatment of PC12 cells leads to changes in the posttranslational processing of the chartin MAPs. The time course of these changes closely resembles that for the increase in the proportion of cellular tubulin that is polymerized and for neurite outgrowth. One of the important events in the growth and stabilization of neurites appears to be the formation of microtubule bundles that extend from the cell body to the tips of the neurites.(ABSTRACT TRUNCATED AT 400 WORDS)

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