Spatio-temporal deviations in fluid flow resulting from particle–fluid interactions

2011 ◽  
Vol 2011 (02) ◽  
pp. P02038 ◽  
Author(s):  
Jesus Felix Valenzuela ◽  
Christopher Monterola
2019 ◽  
Vol 219 (3) ◽  
pp. 1550-1561 ◽  
Author(s):  
J Umlauft ◽  
M Korn

SUMMARY Presently ongoing geodynamic processes within the intracontinental lithospheric mantle give rise to different natural phenomena in the NW Bohemia/Vogtland region (Czech Republic, Germany), amongst others: earthquake swarms, mineral springs and degassing zones of mantle-derived fluids as well as highly concentrated CO2 (mofettes). Their interaction mechanisms and relations are not yet fully understood, but fluid pathways within the crust are assumed, that allow efficient fluid transport between the main hypocentral swarm quake region and the degassing areas at the surface. Here, we focus on the location of the presumed fluid channels as well as on the investigation of their near-surface spatio-temporal variability, targeting a depth of a few hundreds of metmetres. We applied a 3-D matched field processing (MFP) approach in the frequency band of 10–20 Hz considering the fluid flow as seismic noise source. Within three campaigns in 2015/2016, we recorded continuous seismic noise data on the Hartoušov Mofette Field within the Cheb Basin (NW Bohemia, CZ), which is a key site to study fluid flow as it is characterized by strong and continuous surface degassing of CO2. We used temporary arrays varying in extent (70-600 m aperture) and in the amount of stations (25–95 units). Assuming a homogeneous velocity model and applying conventional MFP phase-matching over a 3-D grid search, we located two channel-like structures beneath the test site, which could be traced down to a common source area down to 2000 m depth. We thereby evaluated the influence of amplitude normalization of the measured noise signal on the MFP location considering water-filled or dry mofette channels. Additionally, a spatio-temporal analysis using time windows with a length of 10 min during 5 hr of noise record shows variability of fluid flow activity in space and time and hence, its migration beneath the test site on a short timescale.


2019 ◽  
Vol 877 ◽  
pp. 759-823 ◽  
Author(s):  
Robert A. Van Gorder ◽  
Hyunyeon Kim ◽  
Andrew L. Krause

We study spatial and spatio-temporal pattern formation emergent from reaction–diffusion–advection systems formed by considering reaction–diffusion systems coupled to prescribed fluid flows. While there have been a number of studies on the planar dynamics of such systems and the resulting instabilities and spatio-temporal patterning in the plane, less has been done on complicated flows in complex domains. We consider a general approach for the study of bounded domains in order to model two- and three-dimensional geometries which are more likely to be of relevance for modelling dynamics within fluid vessels used in experiments. Considering a variety of problem geometries with finite cross-sections, such as two-dimensional channels, three-dimensional ducts and three-dimensional pipes, we demonstrate the role cross-section geometry plays in pattern formation under such systems. We find that the generic instability is that of an oscillatory or wave Turing instability, resulting in patterns which change in time, often being advected with the fluid flow. As in previous works, we observe a change in patterns formed when progressing from zero to weak to strong advection for uniform advection across the domain, with particularly strong advection destroying patterns. One novel finding is that heterogeneous fluid flow can induce qualitatively different patterns across the domain. For instance, Poiseuille flow with maximal advection in the centre of a vessel and zero advection at the boundary of a vessel is shown to exhibit patterns in the centre of the vessel which are different from patterns near the boundary, with differences attributed to the differential local advection within each region of the vessel. Additionally, we observe sheared patterns, which appear due to gradients in the fluid velocity, and cannot be obtained via any kind of uniform flow. Finally we also explore flow in more complex domains, including wavy-walled channels, continuous stirred-tank reactors, U-shaped pipes and a toroidal domain, in order to demonstrate behaviours when the flow is both heterogeneous and bidirectional, as well as to demonstrate that our results still apply for complex finite domains. Our analysis suggests that such non-trivial advection results in moving patterns which are more complex than observed in simpler reaction–diffusion–advection, and may be more characteristic of realistic flow regimes in biological media.


2005 ◽  
Vol 41 ◽  
pp. 15-30 ◽  
Author(s):  
Helen C. Ardley ◽  
Philip A. Robinson

The selectivity of the ubiquitin–26 S proteasome system (UPS) for a particular substrate protein relies on the interaction between a ubiquitin-conjugating enzyme (E2, of which a cell contains relatively few) and a ubiquitin–protein ligase (E3, of which there are possibly hundreds). Post-translational modifications of the protein substrate, such as phosphorylation or hydroxylation, are often required prior to its selection. In this way, the precise spatio-temporal targeting and degradation of a given substrate can be achieved. The E3s are a large, diverse group of proteins, characterized by one of several defining motifs. These include a HECT (homologous to E6-associated protein C-terminus), RING (really interesting new gene) or U-box (a modified RING motif without the full complement of Zn2+-binding ligands) domain. Whereas HECT E3s have a direct role in catalysis during ubiquitination, RING and U-box E3s facilitate protein ubiquitination. These latter two E3 types act as adaptor-like molecules. They bring an E2 and a substrate into sufficiently close proximity to promote the substrate's ubiquitination. Although many RING-type E3s, such as MDM2 (murine double minute clone 2 oncoprotein) and c-Cbl, can apparently act alone, others are found as components of much larger multi-protein complexes, such as the anaphase-promoting complex. Taken together, these multifaceted properties and interactions enable E3s to provide a powerful, and specific, mechanism for protein clearance within all cells of eukaryotic organisms. The importance of E3s is highlighted by the number of normal cellular processes they regulate, and the number of diseases associated with their loss of function or inappropriate targeting.


2019 ◽  
Vol 47 (6) ◽  
pp. 1733-1747 ◽  
Author(s):  
Christina Klausen ◽  
Fabian Kaiser ◽  
Birthe Stüven ◽  
Jan N. Hansen ◽  
Dagmar Wachten

The second messenger 3′,5′-cyclic nucleoside adenosine monophosphate (cAMP) plays a key role in signal transduction across prokaryotes and eukaryotes. Cyclic AMP signaling is compartmentalized into microdomains to fulfil specific functions. To define the function of cAMP within these microdomains, signaling needs to be analyzed with spatio-temporal precision. To this end, optogenetic approaches and genetically encoded fluorescent biosensors are particularly well suited. Synthesis and hydrolysis of cAMP can be directly manipulated by photoactivated adenylyl cyclases (PACs) and light-regulated phosphodiesterases (PDEs), respectively. In addition, many biosensors have been designed to spatially and temporarily resolve cAMP dynamics in the cell. This review provides an overview about optogenetic tools and biosensors to shed light on the subcellular organization of cAMP signaling.


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