Antibacterial activity tests of isolate endophytic bacteria from the tea plant (Camellia sinensis) againts Staphylococcus aureus and Staphylococcus epidermidis

Abstract: Jatropha Leaves, Staphylococcus Epidermidis, Staphylococcus Aureus. Jatropha curcas L leaves contain tannins, saponins and flavonoids. According to some studies, tannins, saponins and flavonoids have antibacterial power. Based on these indications, it is important to do research on antibacterial potency of jatropha leaf extract (Jatropha curcas L) against Staphylococcus epidermidis and Staphylococcus aureus bacteria. The aim of this research is to know Antibacterial Activity of Jatropha curcas L Extract on Staphylococcus epidermidis and Staphylococcus aureus". This research. An experimental study by testing the antibacterial activity of leaf extract and jatropha result of maceration, infundation and reflux as measured by Minimal Inhibitory Concentration (KHM) on growth of Staphylococcus epidermidis and Staphylococcus aureus bacteria. From the results of the research, it was found that the extract of the reflux was brownish green and the characteristic of jatropha leaves. The amount of extract produced was 17.03 g, so the yield was 17.03%. Jatropha leaf extract of maceration has no inhibitory power to S. epidermidis in 4 series concentrations starting from 3.125 ppm to 25.000 ppm. Inhibitory zones begin to appear at concentrations of 50,000 ppm and increase at concentrations of 100,000 ppm. The drag zone diameter in the Jatropha curcas extract of the reflux showed the inhibitory power in all series of concentrations. The smallest inhibitory zone was shown at a concentration of 3,125 ppm (9.33 mm) and increased with increasing concentrations. The diameter of the inhibit zone was greatest at 100,000 ppm concentrations of 22.67 mm. Description of inhibitory zone diameter of methanol leaf extract of jatropha result of maceration and reflux to S. epidermidis. The conclusion of this research is extract of reflux result giving bigger resistance than maceration extract to S. epidermidis and S. aureus. Methanol extract of jatropha from the maceration and reflux have strong antibacterial activity against S. epidermidis and S. aureus but still under Klindamycin.

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Antibacterial Activity of Extract and Fraction From Shitake Mushroom (Lentinula edodes) Against Acne Bacteria

Indonesian Journal of Pharmaceutical Science and Technology ◽

10.24198/ijpst.v6i1.14732 ◽

2018 ◽

Vol 6 (1) ◽

pp. 36

Author(s):

Ika Kurnia Sukmawati ◽

Ari Yuniarto ◽

Widhya Alighita ◽

Ade Zam-zam J

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Staphylococcus Epidermidis ◽

Propionibacterium Acnes ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Lentinus Edodes ◽

Shiitake Mushrooms ◽

And Staphylococcus Aureus

Acne is an inflammatory disease that occurs in the skin triggered by the bacteria acne treatment can be done by using natural materials that shiitake mushrooms (Lentinus edodes). This aims of this study were to determining the antibacterial activity of extracts and fractions shiitake mushrooms with broth microdilution method, determining the value of equality shiitake mushrooms with antibacterial comparison and determining the morphological changes of bacteria after exposure to the test sample with a Scanning Electron Microscope (SEM). Tests conducted antibacterial activity against Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at concentrations used is 1 ppm, 2 ppm, 4 ppm, 8 ppm, 16 ppm, 32 ppm, 64 ppm, 128 ppm, 256 ppm, 512 ppm. Best MIC value obtained in fraction of ethyl acetate and n-hexane fraction of the bacterium Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at a concentration of 256 ppm. KBM value of the n-hexane fraction against Staphylococus aureus bacteria at concentrations of 512 ppm and ethyl acetate fraction against bacteria Staphylococus aureus and epidermidis at a concentration of 512 ppm. Value equality is obtained 1 mg of ethyl acetate fraction of shiitake mushrooms equivalent to 5.346 x 10-2 mg of tetracycline. SEM test results showed the presence of antibacterial activity which is indicated by a change in cell morphology, their lumps and their cell wall frown on Propionibacterium acnes were exposed to ethyl acetate fraction.Key words: Acne, Lentinus edodes, antibacterials, microdilution, SEM

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Antibacterial activity of the dregs of green tea leaves (Camellia sinensis L.) on Staphylococcus epidermidis as causes of acne

Journal of Science & Science Education ◽

10.24246/josse.v1i2p1-5 ◽

2017 ◽

Vol 1 (2) ◽

pp. 1-5

Author(s):

Naniek Widyaningrum ◽

Badie'ah Badie'ah ◽

Sri Lestari

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Green Tea ◽

Camellia Sinensis ◽

Staphylococcus Epidermidis ◽

Economic Value ◽

Diffusion Method ◽

Tea Leaves ◽

Green Tea Leaves

The dregs of green tea leaves have not been used until today, it is still considered as a waste that is not useful. It is known that the dregs of the green tea leaves contain Epigallocatechin-3-gallate (EGCG), which is quite high. EGCG has benefits as an antibacterial. The long-term goal of this research is the application of cosmetic preparation products from the dregs of green tea leaves that have effectiveness, stability and high economic value. The specific target of this research is to get the dose and concentration of the dregs of green tea leaves in a stable and effective way as anti-acne against Staphylococcus epidermidis (S. epidermidis). The search of the dregs of green tea leaves is by techniques of infundation of liquid fractions with ethyl acetate, identification of EGCG compound by using HPLC, antibiotics test against S. epidermidis was conducted by diffusion method. The results showed that the content of EGCG of the dregs of green tea leaves was 21.325% w/w, and the concentration of 1% of the dregs of green tea leaves can inhibit the growth of S. epidermidis well.

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IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF 1.5-BIS (3’-ETHOXY-4’-HYDROXYPHENYL)-1-4-PENTADIENE-3-ONE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i5.25143 ◽

2018 ◽

Vol 10 (5) ◽

pp. 70

Author(s):

Agus Purwanggana ◽

Esti Mumpuni ◽

Esti Mulatsari

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Staphylococcus Epidermidis ◽

In Silico ◽

Inhibitory Concentration ◽

Inhibition Zone ◽

Antibacterial Study

Objective: The main objective of this research were screened in vitro and in silico of 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one as potential antibacterial agents.Methods: The in vitro antibacterial study was carried against Staphylococcus aureus, Staphylococcus epidermidis (gram positive) and Escherichia coli, Salmonella thypi (gram negative) using broth dilution method to determine Minimum Inhibitory Concentration (MIC), disc diffusion method to determine the diameter of inhibition zone. In silico antibacterial study was carried using computational software Protein-Ligand ANT System (PLANTS), computational docking was carried using receptor with Protein Data Bank (PDB) file 3MZD. The structures were optimized prior docking using YASARA, and MarvinSketch. The results of antibacterial testing were compared to two positive control drugs i. e amoxicillin and cefadroxil.Results: In vitro evaluation showed that 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has a better antibacterial activity than amoxicillin and cefadroxil with a Minimum Inhibitory Concentration (MIC) of 0.15 ppm and diameter of inhibition zone of 11.27±0.31, 11.35±0.39, 11.25±0.33, and 11.05±0.45 mm in Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Salmonella thypi, respectively. These results in line with in silico evaluation that showed 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has more negative docking score than amoxicillin, cefadroxil, and cloxacillin acyl as a native ligand on the 3MZD receptor.Conclusion: This results obtained in this research work were 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one compound potential as an antibacterial agent.

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