scholarly journals IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF 1.5-BIS (3’-ETHOXY-4’-HYDROXYPHENYL)-1-4-PENTADIENE-3-ONE

2018 ◽  
Vol 10 (5) ◽  
pp. 70
Author(s):  
Agus Purwanggana ◽  
Esti Mumpuni ◽  
Esti Mulatsari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Staphylococcus Epidermidis ◽  
In Silico ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Antibacterial Study

Objective: The main objective of this research were screened in vitro and in silico of 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one as potential antibacterial agents.Methods: The in vitro antibacterial study was carried against Staphylococcus aureus, Staphylococcus epidermidis (gram positive) and Escherichia coli, Salmonella thypi (gram negative) using broth dilution method to determine Minimum Inhibitory Concentration (MIC), disc diffusion method to determine the diameter of inhibition zone. In silico antibacterial study was carried using computational software Protein-Ligand ANT System (PLANTS), computational docking was carried using receptor with Protein Data Bank (PDB) file 3MZD. The structures were optimized prior docking using YASARA, and MarvinSketch. The results of antibacterial testing were compared to two positive control drugs i. e amoxicillin and cefadroxil.Results: In vitro evaluation showed that 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has a better antibacterial activity than amoxicillin and cefadroxil with a Minimum Inhibitory Concentration (MIC) of 0.15 ppm and diameter of inhibition zone of 11.27±0.31, 11.35±0.39, 11.25±0.33, and 11.05±0.45 mm in Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Salmonella thypi, respectively. These results in line with in silico evaluation that showed 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has more negative docking score than amoxicillin, cefadroxil, and cloxacillin acyl as a native ligand on the 3MZD receptor.Conclusion: This results obtained in this research work were 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one compound potential as an antibacterial agent. 

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

scholarly journals AKTIVITAS SEDIAAN GEL KUERSETIN TERHADAP Staphylococcus Epidermidis

2015 ◽  
Vol 1 (1) ◽  
pp. 59-64
Author(s):  
Rina Apriana Herslambang ◽  
Dina Rahmawanty ◽  
Mia Fitriana
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Phenolic Compound ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Sebaceous Glands ◽  
Agar Diffusion ◽  
Agar Diffusion Method

Acne is the abnormality production of sebum in the sebaceous glands which are usually caused by Staphylococcus epidermidis. Staphylococcus epidermidis one of bacterial that caused acne. Quercetin have a phenolic compound that can inhibit the growth of Staphylococcus epidermidis at 0.05% w/w. The purpose of this study was to determine the minimum inhibitory concentration of quercetin gel. HPMC was used as gel base. The concentrations of quercetin that was added to gel were 0.05% w/w, 0.15% w/w, and 0.25% w/w. The antibacterial activity of the gels was determined using agar diffusion method. The results showed that the inhibition zone on the F1, F2, and F3 were 7.83 mm , 6.53 mm , 4.56 mm and the result showed that quercetin gels significantly effected the growth of Staphylococcus epidermidis (p<0,05). The minimum inhibitory concentration of quercetin gel was 0,05% w/w with inhibition zone of 7.83 mm.

scholarly journals Antibacterial Activity of Murrayaquinone A and 6-Methoxy-3,7-dimethyl-2,3-dihydro-1H-carbazole-1,4(9H)-dione

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Biswanath Chakraborty ◽  
Suchandra Chakraborty ◽  
Chandan Saha
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Antibacterial Activities ◽  
Gram Positive ◽  
Gram Negative ◽  
Naturally Occurring

The antibacterial activity of Murrayaquinone A (10), a naturally occurring carbazoloquinone alkaloid, and 6-methoxy-3,7-dimethyl-2,3-dihydro-1H-carbazole-1,4(9H)-dione (11), a synthetic carbazoloquinone, both obtained during the development of the synthesis of Carbazomycin G, having unique quinone moiety, was studied against Gram-positive (Bacillus subtilisandStaphylococcus aureus) and Gram-negative (Escherichia coliandPseudomonassp.) bacteria. Compound10showed antibacterial activities against both ofEscherichia coliandStaphylococcus aureuswhereas compound11indicated the activity againstStaphylococcus aureusonly. Both compounds10and11exhibited minimum inhibitory concentration (MIC) of 50 μg mL−1againstStaphylococcus aureus.

scholarly journals Antibacterial Activity of Cinnamon Extract (Cinnamomum burmannii) against Staphylococcus aureus and Escherichia coli In Vitro

2019 ◽  
Vol 3 (2) ◽  
pp. 19-28
Author(s):  
Nita Parisa ◽  
Rahma Nur Islami ◽  
Ella Amalia ◽  
Mariana Mariana ◽  
Riana Sari Puspita Rasyid
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Control Group ◽  
Antibacterial Compounds ◽  
Cinnamon Extract ◽  
Cinnamomum Burmannii

Abstract   Infectious disease is one of the most common diseases in the world. Staphylococcus aureus and Escherichia coli are two common causes of infection and are resistant to many antibiotics, so the new agents are needed to overcome antibiotic resistance. Cinnamon is often used as a preservative because it has antibacterial activity. Cinnamomum burmannii is kind of native cinnamon from Indonesia. The antimicrobial active compounds cinnamaldehyde and eugenol are the main reasons for its antibacterial activity. This study observed the efficacy of the cinnamon extract (Cinnamomum burmannii) as antibacterial against Staphylococcus aureus and Escherichia coli. An experimental study, in vitro using Post-test Only Control Group Designed, has been done in Microbiology and Biotechnology Laboratory of Medical Faculty of Sriwijaya University. Cinnamon was extracted, then tested for its antibacterial activity using well diffusion and serial dilution to determine diameter of inhibition zone and minimum bactericidal concentration. Phytochemical tests were also conducted to determine the antibacterial compounds of cinnamon extract. Ethanol extract of cinnamon was able to inhibit the growth of Staphylococcus aureus with MBC 5% and inihibitory zone 6,84±0,68 mm and Escherichia coli with MBC 10% and inhibitory zone 5,69±0,69 mm. Cinnamon extract which has the greatest effectiveness is concentration of 40% with inhibition zone 15,69±0,80 mm (Staphylococcus aureus) and 9,63±0,59 mm (Escherichia coli). This ability is due to the antibacterial compounds as evidenced by positive results in various phytochemical tests. Cinnamon extract is effective as antibacterial against Staphylococcus aureus and Escherichia coli in vitro.   Keywords: efficacy, antibacterial, Cinnamomum burmannii, Staphylococcus aureus, Escherichia coli

scholarly journals Phytochemical characterization, antioxidant and antibacterial activity of essential oil and extracts of Tagetes patula on Staphylococcus aureus

2020 ◽  
Vol 37 (4) ◽  
pp. 347-367
Author(s):  
Virginia Monserrate López Zambrano ◽  
Alex Alberto Dueñas Rivadeneira ◽  
José Gerardo Cuenca Nevárez ◽  
Joan Manuel Rodríguez-Díaz
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Refractive Index ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
In Vitro Tests ◽  
Tagetes Patula

Tagetes patula is an ornamental plant species and its essential oils contain potentially allelopathic active ingredients. The objective of the present study was to evaluate the phytochemical composition of extracts, the antioxidant and antibacterial activity of the essential oil of flowering leaves of the species, on Staphylococcus aureus. For this, the extraction of the essential oil by hydrodistillation was carried out, evaluating the physical characteristics (solubility, density and refractive index) and phytochemical characteristics of the extracts through a phytochemical screening (alkaloids, flavonoids, phenols, saponins, tannins and reducing sugars). The quantificatics of phenols in the essential oil was performed by the Folin Ciocalteu method, the antioxidant activity using the DPPH and ABTS tests, the antimicrobial activity by determining the minimum inhibitory concentration. The results obtained showed for the essential oil, a density of 0,733 g.mL-1, refractive index of 1,47 and insolubility in ethanol (70 %), presence of tannins, flavonoids and phenols in the extracts. The phenolic content was 1.024 ± 0,19 mg.g-1 TAE, the antioxidant activity with DPPH was 87,6 ± 0,18 µmol.g-1TE and with ABTS 180,83 ± 0,36 µmol.g-1 TE in Trolox equivalent. The minimum inhibitory concentration was 16,67 mm against S. aureus, in response to which it is concluded that the essential oil of T. patula had antioxidant activity against radicals DPPH and ABTS, high phenolic contents and showed antibacterial activity in vitro tests against S. aureus.

scholarly journals Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

2020 ◽  
pp. 795
Author(s):  
Rania Benjamaa ◽  
Abdelkarim Moujanni ◽  
Anass Terrab ◽  
Rabiaa Eddoha ◽  
Maryam Benbachir ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Antibiotic Residues ◽  
Antibiotic Resistant ◽  
Liquid Chromatography Tandem Mass

Antibiotic-resistant bacteria continue to be of major health concern worldwide. In recent years, several reports and scientific articles claim the contamination of honey by antibiotics, detectable concentrations of antibiotic residues in honey are illegal. They, may cause hypersensitivity or resistance to drug therapy in humans, and are perceived by consumers as undesirable. In this sense, the purpose of this work was to examine the antibacterial activity of the Euphorbia resinifera (E. resinifera) honey against Escherichia coli and Staphylococcus aureus in vitro using the well-agar diffusion assay followed by dilution range to obtain more precise minimum inhibitory concentration values. The second aim is to evaluate the presence of antibiotics in honey using a screening test: Evidence InvestigatorTM, an immuno-enzymatic method for detection of 27 antibiotic residues followed by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) for confirmation of suspect samples; in order to assess the relationship between the presence of antibiotic residues and the antibacterial activity of honey. In this study, a total of 37 E. resinifera honey samples were analyzed. The results show that all samples of honey inhibited the growth of bacteria at the dilutions at 50% (v/v); the highest inhibition zone (25.98 ± 0.11 mm) was recorded from sample 5 for Staphylococcus aureus and (13.84 ± 1.10 mm) in sample 17 for Escherichia coli and that 50% (v/v) dilutions showed significant antibacterial effect compared to other dilutions (6.25, 12.5, 25% (v/v)). In all samples, there were no antibiotic residues detected except for one showing the detection of Trimethoprim at 6.48 µg kg-1. Our research is one of the first studies that relate the he relationship between the presence of antibiotic residues and the antibacterial activity of Euphorbia resinifera honey and showed that the antibacterial activity of honey might be due to the high osmotic nature, a low pH, its content of phenolic compounds and hydrogen peroxide and also to its content of methylglyoxal.

scholarly journals THE ANTIBACTERIAL ACTIVITY OF WATER APPLE LEAVES ACTIVE COMPOUND (Syzygium zeylanicum) AGAINST Escherichia coli AND Staphylococcus aureus

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Hamidah ◽  
Salni ◽  
Nina Tanzerina
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Active Compound ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Active Compounds ◽  
Apple Leaves ◽  
And Staphylococcus Aureus

Escherichia coli is one of the bacteria that cause infections in the human digestive tract such as diarrhea, while Staphylococcus aureus is one of the bacteria that cause infections in the skin injury such as boils and pimples. This study used Syzygium zeylanicum leaves because it has potential as a antibacterial because it contains active compounds. This study aimed was determine the antibacterial activity of the fraction and the active compound in Syzygium zeylanicum leaves against Escherichia coli and Staphylococcus aureus. Research conducted on November 2015 to January 2016. The method used in this research were extraction by maceration, fractionation by liquid fractionation, antibacterial activity test, and determination of minimum inhibitory concentration with the diffusion method and isolation of active compounds by column chromatography method. The bacteria used in this test are Escherichia coli and Staphylococcus aureus. Data are presented in tabular form based on the average value of the inhibition diameter and deviation standard. The results of this research showed the water methanol active fraction against the bacteria that used in this test. The methanol water fraction had obtained one antibacterial compound in bottle 1,3,5 which shows the value of tannin Rf 0,416. The minimum inhibitory concentration of water methanol of water apple leaves is 1000 µg/mL for Escherichia coli and 500 µg/mL for  Staphylococcus aureus. The minimum  inhibitory concentration of the active  compound  to  Escherichia  coli  and  Staphylococcus  aureus  in  500  µg/mL.  The fraction and the active compound of water apple leaves have an antibacterial activity with Escherichia coli and Staphylococcus aureus and the active compound is tannin.

scholarly journals The In Vitro Susceptibility of Biofilm Forming Medical Device Related Pathogens to Conventional Antibiotics

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Garry Laverty ◽  
Mahmoud Y. Alkawareek ◽  
Brendan F. Gilmore
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Broth Microdilution ◽  
In Vitro Susceptibility ◽  
Conventional Antibiotics ◽  
Kinetics Of

Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum biofilm eradication concentration (MBEC) and kill kinetics were established for vancomycin, rifampicin, trimethoprim, gentamicin, and ciprofloxacin against the biofilm forming bacteria Staphylococcus epidermidis (ATCC 35984), Staphylococcus aureus (ATCC 29213), Methicillin Resistant Staphylococcus aureus (MRSA) (ATCC 43300), Pseudomonas aeruginosa (PAO1), and Escherichia coli (NCTC 8196). MICs and MBCs were determined via broth microdilution in 96-well plates. MBECs were studied using the Calgary Biofilm Device. Values obtained were used to investigate the kill kinetics of conventional antimicrobials against a range of planktonic and biofilm microorganisms over a period of 24 hours. Planktonic kill kinetics were determined at 4xMIC and biofilm kill kinetics at relative MBECs. Susceptibility of microorganisms varied depending on antibiotic selected and phenotypic form of bacteria. Gram-positive planktonic isolates were extremely susceptible to vancomycin (highest MBC: 7.81 mg L−1: methicillin sensitive and resistant S. aureus) but no MBEC value was obtained against all biofilm pathogens tested (up to 1000 mg L−1). Both gentamicin and ciprofloxacin displayed the broadest spectrum of activity with MIC and MBCs in the mg L−1 range against all planktonic isolates tested and MBEC values obtained against all but S. epidermidis (ATCC 35984) and MRSA (ATCC 43300).

scholarly journals Syntheses and Antibacterial Studies of Some 1-Phenyl-3-(4-(2-ethanoloxy) phenyl)-5-aryl-1H-pyrazoles

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Anju Goyal ◽  
Sandeep Jain
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Phenyl Hydrazine ◽  
Gram Negative ◽  
H Nmr ◽  
Antibacterial Studies

A series of 1-phenyl-3-(4-(2-ethanoloxy) phenyl)-5-aryl-1H-pyrazoles were synthesized from chalcones, that is, 3-aryl-1-(4-hydroxyphenyl) prop-2-en-1-ones and studied for theirin vitroantibacterial activity. Chalcones1on reaction with phenyl hydrazine in the presence of acetic acid and few drops of hydrochloric acid yielded the corresponding 1-phenyl-3-(4-hydroxyphenyl)-5-aryl-1H-pyrazoles2which on further reaction with 2-chloroethanol furnished the title compounds3. These compounds were characterized by CHN analyses, IR, mass and1H NMR spectral data. All the compounds were evaluated for theirin vitroantibacterial activity against two Gram positive strains (Bacillus subtilisandStaphylococcus aureus) and two Gram negative strains (Escherichia coliandPseudomonas aeruginosa), and their minimum inhibitory concentration (MIC) was determined.

scholarly journals Synthesis and Antibacterial Testing of Imina Derivative Compounds from Piperonal and Anilin

2018 ◽  
Vol 21 (1) ◽  
pp. 44-48 ◽  
Author(s):  
Dimas Syamsudin ◽  
Ismiyarto Ismiyarto ◽  
Ngadiwiyana Ngadiwiyana
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Melting Point ◽  
1H Nmr ◽  
Antibacterial Agent ◽  
Inhibitory Concentration ◽  
Condensation Reaction ◽  
Inhibition Zone

Synthesis of Imine derivates used as antibacterial activity has been done. Synthesis was conducted by condensation reaction between piperonal and aniline. The product synthesis was characterized by melting point, LC-MS, FTIR and 1H-NMR, then application of the product as antibacterial towards bacteria Staphylococcus aureus and Escherichia coli. The product of synthesis was pale-brown solid, and had melting point at 56-61 0C. Identification by LC-MS showed the compound molecular weight was 225.30 gram/mol, identification by FTIR showed a compound contained imine C=N at 1620.21 cm-1, C-H aromatic at 1581.63 cm-1, Csp3-H at 2962.37 cm-1 from CH2, and C-O at 1265.30 cm-1, Identification by 1H-NMR showed a compound had a singlet peak at δ=6 ppm that proton was from the CH2, multiplet peak at δ=6.9-7.7 ppm proton from two aromatics and singlet peak at δ=8.4 ppm from proton imine. The minimum inhibitory concentration of the product towards Staphylococcus aureus and Escherichia coli was 1 mg/mL with diameter inhibition zone 2 mm and 3 mm. From these data it could be concluded that the compound 3,4- methlenedioxybenzilyimine was formed and it had ability as an antibacterial agent.

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