Optical Design of A CaF2-free 5× Flat-field Apochromatic Metallographic Micro-objective with Long Working Distance

Two transmission curved crystal spectrometers are designed to measure the hard x-ray emission in the laser fusion experiment of Compton radiography of implosion target on ShenGuang-III laser facility in China. Cylindrically curved ${\it\alpha}$ -quartz (10–11) crystals with curvature radii of 150 and 300 mm are used to cover spectral ranges of 10–56 and 17–100 keV, respectively. The distance between the crystal and the x-ray source can be changed over a broad distance from 200 to 1500 mm. The optical design, including the integral reflectivity of the curved crystal, the sensitivity, and the spectral resolution of the spectrometers, is discussed. We also provide mechanic design details and experimental results using a Mo anode x-ray source. High-quality spectra were obtained. We confirmed that the spectral resolution can be improved by increasing the working distance, which is the distance between the recording medium and the Rowland circle.

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Optical design of CCF PlanApo microobjectives 50 mm observation and super long working distance

Current Developments in Lens Design and Optical Engineering XIX ◽

10.1117/12.2319840 ◽

2018 ◽

Author(s):

Dmitry N. Frolov ◽

Alexey D. Frolov ◽

Olga A. Vinogradova ◽

Liyun Wen ◽

Fanhua Jing

Keyword(s):

Optical Design ◽

Working Distance

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EnLightenment: High resolution smartphone microscopy as an educational and public engagement platform

Wellcome Open Research ◽

10.12688/wellcomeopenres.12841.2 ◽

2018 ◽

Vol 2 ◽

pp. 107 ◽

Cited By ~ 4

Author(s):

Laura C. Wicks ◽

Gemma S. Cairns ◽

Jacob Melnyk ◽

Scott Bryce ◽

Rory R. Duncan ◽

...

Keyword(s):

Citizen Science ◽

Public Engagement ◽

Marine Biology ◽

Low Cost ◽

Single Cells ◽

Optical Design ◽

Cost Effective ◽

Objective Lens ◽

Working Distance ◽

The Cost

We developed a simple, cost-effective smartphone microscopy platform for use in educational and public engagement programs. We demonstrated its effectiveness, and potential for citizen science through a national imaging initiative, EnLightenment. The cost effectiveness of the instrument allowed for the program to deliver over 500 microscopes to more than 100 secondary schools throughout Scotland, targeting 1000’s of 12-14 year olds. Through careful, quantified, selection of a high power, low-cost objective lens, our smartphone microscope has an imaging resolution of microns, with a working distance of 3 mm. It is therefore capable of imaging single cells and sub-cellular features, and retains usability for young children. The microscopes were designed in kit form and provided an interdisciplinary educational tool. By providing full lesson plans and support material, we developed a framework to explore optical design, microscope performance, engineering challenges on construction and real-world applications in life sciences, biological imaging, marine biology, art, and technology. A national online imaging competition framed EnLightenment; with over 500 high quality images submitted of diverse content, spanning multiple disciplines. With examples of cellular and sub-cellular features clearly identifiable in some submissions, we show how young public can use these instruments for research-level imaging applications, and the potential of the instrument for citizen science programs.

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Astronomy with the Automated Patrol Telescope

International Astronomical Union Colloquium ◽

10.1017/s0252921100021667 ◽

1995 ◽

Vol 148 ◽

pp. 44-47

Author(s):

B. D. Carter ◽

M. C. B. Ashley ◽

C. S. Bembrick ◽

P. W. Brooks ◽

P. Mitchell ◽

...

Keyword(s):

New South ◽

Computer Control ◽

Optical Design ◽

Open Clusters ◽

Electronic Systems ◽

Wide Field ◽

Ccd Imaging ◽

South Wales ◽

Flat Field ◽

The University

AbstractThe Automated Patrol Telescope (APT) is a wide-field CCD imaging telescope operated by the University of New South Wales at Siding Spring Observatory in Australia. The optical design employed resembles that of a Schmidt, but uses a 3-element lens to achieve a wide, corrected field of view. The APT was developed by extensively modifying the optical, mechanical and electronic systems of a Baker-Nunn satellite tracking camera. Telescope motion and operation of the CCD have been placed under computer control, allowing automated observations for longterm survey and monitoring projects. The APT has 0.5 m aperture f/1 optics which produce a 5° flat field, of which a 2°×3° field is covered by the CCD currently installed. The telescope is being used for studies of stellar activity in open clusters and regions of star formation, and comet and minor planet investigations. A number of other projects for the APT are being considered, including searches for novae, supernovae in clusters of galaxies, and brown dwarfs.

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Towards an extremely high resolution broad-band flat-field spectrometer in the `water window'

Journal of Synchrotron Radiation ◽

10.1107/s1600577519004648 ◽

2019 ◽

Vol 26 (4) ◽

pp. 1058-1068

Author(s):

Zhuo Li ◽

Bin Li

Keyword(s):

High Efficiency ◽

Optical Design ◽

Broad Band ◽

Resolving Power ◽

High Spectral Resolution ◽

Optical Aberrations ◽

Water Window ◽

Flat Field ◽

Line Spacing ◽

Sase Fel

The optical design of a novel spectrometer is presented, combining a cylindrically convex pre-mirror with a cylindrically concave varied-line-spacing grating (both in the meridional) to deliver a resolving power of 100000–200000 in the `water window' (2–5 nm). Most remarkably, the extremely high spectral resolution is achieved for an effective meridional source size of 50 µm (r.m.s.); this property could potentially be applied to diagnose SASE-FEL and well resolve individual single spikes in its radiation spectrum. The overall optical aberrations of the system are well analysed and compensated, providing an excellent flat-field at the detector domain throughout the whole spectral range. Also, a machine-learning scheme – SVM – is introduced to explore and reconstruct the optimal system with high efficiency.

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Design and Simulation of an Ultra-Wide Field Optical Coherence Tomography Retinal Imaging System

Photonics ◽

10.3390/photonics8110476 ◽

2021 ◽

Vol 8 (11) ◽

pp. 476

Author(s):

Shanshan Liang ◽

Xinyu Li ◽

Jiajing Kang ◽

Mingming Wan ◽

Jiahui Wang ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Imaging System ◽

Optical Design ◽

Retinal Imaging ◽

Wide Field ◽

Optical Coherence ◽

Tomography System ◽

Imaging Simulation ◽

Unique Approach ◽

Working Distance

Peripheral retinal imaging is a unique approach for assessing and monitoring ocular diseases. In this paper, we proposed a design for an optical coherence tomography system to accomplish ultrawide field (>120°) retinal imaging without montages. Scanning of the sample arm was achieved via two ellipsoidal mirrors. The optical design software Zemax and an eye model were used to estimate the inherent aberrations in the system and the optical performance of retinal imaging. Simulation results of the aberrations in the designed system indicated that the designed system can achieve an unprecedented imaging field of view (FOV) while maintaining acceptable resolution without sacrificing the working distance. This work suggests that ultrawide field optical coherence tomography retinal imaging is achievable, which is highly important for the diagnosis and treatment of ocular—especially peripheral—retinopathy.

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Compact and contactless reflectance confocal microscope for neurosurgery

10.1101/2020.05.13.093435 ◽

2020 ◽

Author(s):

Jiahe Cui ◽

Raphaël Turcotte ◽

Karen M. Hampson ◽

Matthew Wincott ◽

Carla C. Schmidt ◽

...

Keyword(s):

Near Infrared ◽

Optical Design ◽

Hippocampal Slices ◽

Infrared Region ◽

Confocal Microscope ◽

Cellular Level ◽

Tumour Resection ◽

Neurosurgical Procedures ◽

Label Free ◽

Working Distance

AbstractVisual guidance at the cellular level during neurosurgical procedures is essential for complete tumour resection. We present a compact reflectance confocal microscope with a 20 mm working distance that provided <1.2 µm spatial resolution over a 600 µm × 600 µm field of view in the near-infrared region. A physical footprint of 200 mm × 550 mm was achieved using only standard off-the-shelf components. Theoretical performance of the optical design was first evaluated via commercial Zemax software. Then three specimens from rodents: fixed brain, frozen calvaria and live hippocampal slices, were used to experimentally assess system capability and robustness. Results show great potential for the proposed system to be translated into use as a next generation label-free and contactless neurosurgical microscope.

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A Novel Living-Cell Imaging System Design with Ultra-Long Working Distance and High Numerical Aperture

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.364-366.1077 ◽

2007 ◽

Vol 364-366 ◽

pp. 1077-1082

Author(s):

Guo Liang Huang ◽

Zhong Hua Dong ◽

Cheng Deng ◽

Shu Kuan Xu ◽

Jiang Zhu ◽

...

Keyword(s):

Optical Imaging ◽

Imaging System ◽

Optical Design ◽

Numerical Aperture ◽

Living Cells ◽

Culture Solution ◽

High Numerical Aperture ◽

Cells In Culture ◽

Working Distance ◽

Living Cell Imaging

Microscopy is an important tool in biology and medicine, but it is often limited to optical imaging structures with high numerical aperture(NA) with a short working distance(wd), for example NA = 0.6 and wd <1 mm are usual. The common microscope objective is inadequate for imaging of living cells in culture as an optical imaging structure with both high numerical aperture and long working distance is required. In this study, a novel optical design has been developed to meet the long working distance and high resolution power imaging of living cells in a vessel with a high culture solution thickness, where cells need to be developed in about 48 hours or a week. The developed optical design was characterized by an ultra-long working distance (wd >13.5 mm) and high numerical aperture (NA = 0.7). This optical imaging system is not only good for the subcellular imaging of free-floating cells in culture, but also for the imaging of cells attached at a surface of vessel.

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EnLightenment: High resolution smartphone microscopy as an educational and public engagement platform

Wellcome Open Research ◽

10.12688/wellcomeopenres.12841.1 ◽

2017 ◽

Vol 2 ◽

pp. 107 ◽

Cited By ~ 2

Author(s):

Laura C. Wicks ◽

Gemma S. Cairns ◽

Jacob Melnyk ◽

Scott Bryce ◽

Rory R. Duncan ◽

...

Keyword(s):

Citizen Science ◽

Public Engagement ◽

Marine Biology ◽

Low Cost ◽

Single Cells ◽

Optical Design ◽

Cost Effective ◽

Objective Lens ◽

Working Distance ◽

The Cost

We developed a simple, cost-effective smartphone microscopy platform for use in educational and public engagement programs. We demonstrated its effectiveness, and potential for citizen science through a national imaging initiative, EnLightenment. The cost effectiveness of the instrument allowed for the program to deliver over 500 microscopes to more than 100 secondary schools throughout Scotland, targeting 1000’s of 12-14 year olds. Through careful, quantified, selection of a high power, low-cost objective lens, our smartphone microscope has an imaging resolution of microns, with a working distance of 3 mm. It is therefore capable of imaging single cells and sub-cellular features, and retains usability for young children. The microscopes were designed in kit form and provided an interdisciplinary educational tool. By providing full lesson plans and support material, we developed a framework to explore optical design, microscope performance, engineering challenges on construction and real-world applications in life sciences, biological imaging, marine biology, art, and technology. A national online imaging competition framed EnLightenment; with over 500 high quality images submitted of diverse content, spanning multiple disciplines. With examples of cellular and sub-cellular features clearly identifiable in some submissions, we show how young public can use these instruments for research-level imaging applications, and the potential of the instrument for citizen science programs.

Download Full-text

Flat-field illumination for quantitative fluorescence imaging

10.1101/297242 ◽

2018 ◽

Cited By ~ 1

Author(s):

Ian Khaw ◽

Benjamin Croop ◽

Jialei Tang ◽

Anna Möhl ◽

Ulrike Fuchs ◽

...

Keyword(s):

Quantitative Analysis ◽

Fluorescence Microscopy ◽

Single Molecule ◽

Spatial Coherence ◽

Optical Component ◽

Wide Field ◽

Gaussian Profile ◽

Flat Field ◽

Working Distance ◽

Quantitative Fluorescence

AbstractThe uneven illumination of a Gaussian profile makes quantitative analysis highly challenging in laser-based wide-field fluorescence microscopy. Here we present flat-field illumination (FFI) where the Gaussian beam is reshaped into a uniform flat-top profile using a high-precision refractive optical component. The long working distance and high spatial coherence of FFI allows us to accomplish uniform epi and TIRF illumination for multi-color single-molecule imaging. In addition, high-throughput borderless imaging is demonstrated with minimal image overlap.

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