scholarly journals A sandwich-type optical immunosensor based on the alkaline phosphatase enzyme for Salmonella thypimurium detection

2018 ◽  
Vol 131 ◽  
pp. 012039
Author(s):  
E Widyastuti ◽  
M F Puspitasari Schonherr ◽  
A Masruroh ◽  
R A Anggraeni ◽  
Y K Nisak ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Sandwich Type ◽  
Optical Immunosensor ◽  
Phosphatase Enzyme

Choriogonadotropin measured with the Tandem-E immunoenzymetric assay system.

1985 ◽  
Vol 31 (3) ◽  
pp. 441-444 ◽  
Author(s):  
J L Bock ◽  
J Furgiuele ◽  
J C Segen
Keyword(s):  
Alkaline Phosphatase ◽  
Linear Response ◽  
Beta Subunit ◽  
Good Precision ◽  
False Positive Result ◽  
Range Correlation ◽  
Sandwich Type ◽  
Plastic Bead ◽  
Substrate Hydrolysis ◽  
Higher Sensitivity

Abstract We evaluated the Hybritech Tandem-E procedure for quantifying choriogonadotropin (hCG) in human serum. In this "sandwich"-type assay, two monoclonal antibodies directed against different regions of the hCG molecule are used, one coated on a plastic bead, the second conjugated to alkaline phosphatase. The assay can detect as little as 1.0 int. unit of the hormone per liter, shows a linear response up to at least 200 int. units/L, and has good precision. By prolonging the incubations for formation of the sandwich and for substrate hydrolysis, one can achieve higher sensitivity at the expense of a narrower linear range. Correlation with a conventional radioimmunoassay for the beta subunit of hCG was generally excellent, but in one instance the Tandem-E gave an apparently false positive result.

Acute Hepatopathy Associated With Mitotane Administration in a Dog

2006 ◽  
Vol 42 (4) ◽  
pp. 298-301 ◽  
Author(s):  
Craig B. Webb ◽  
David C. Twedt
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Supportive Care ◽  
Prothrombin Time ◽  
Liver Enzyme ◽  
Hepatic Failure ◽  
Enzyme Activities ◽  
Total Bilirubin ◽  
Persistent Elevation ◽  
Phosphatase Enzyme

An adult dog with a persistent elevation in alkaline phosphatase enzyme activity was started on mitotane for suspected hyperadrenocorticism. One month later, the dog was presented for intermittent anorexia and acute icterus. The dog’s liver enzyme activities and total bilirubin were markedly elevated, prothrombin time was prolonged, and blood urea nitrogen and glucose were low. Histopathology revealed marked, centrilobular, hepatocellular loss. After discontinuation of the mitotane, the dog recovered with supportive care and was normal 3 months later, which was consistent with mitotane-associated hepatic failure.

scholarly journals Assessment of the microbiological activity in agricultural and urban soils

2014 ◽  
Vol 65 (4) ◽  
pp. 156-160 ◽  
Author(s):  
DOROTA DEC
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Dehydrogenase Activity ◽  
Urban Soil ◽  
Urban Soils ◽  
Agricultural Soils ◽  
C:N Ratio ◽  
Soil Samples ◽  
Microbiological Activity ◽  
Phosphatase Enzyme

Abstract The aim of this study was to evaluate the enzymatic soil and the number of selected microorganisms in urban soil, which are located in the lane of the reconstructed road and compare it with a soil cultivated for agricultural purposes. The conducted analysis showed significant differences between the results of the soil taken from the roadway and the soil cultivated from agricultural purposes. The C:N ratio in soils of the roadway (from 24 to 31) indicated that they were degraded and heavily degraded soils. Urban soils had a neutral pH. The activity of dehydrogenase (1.93–6.95 μg TPF g−1·h−1), acid phosphatase (2.42–4.92 mM pNP·g−1·h−1) and alkaline phosphatase (2.34–4.80 mM pNP·g−1·h−1) in urban soils were low. In agricultural soils the acid phosphatase enzyme levels ranged 6.32–8.04 mM pNP·g−1·h−1, and alkaline phosphatase were 7.26–9.16 mM pNP·g−1·h−1. In urban soil samples collected along the roadway, a significant correlation between potassium and dehydrogenase activity, and between the C:N ratio and the activity of acid phosphatase was found.

Serum Total Alkaline Phosphatase Enzyme Levels in Chronic Periodontitis Patients Among Tamilnadu Population

2012 ◽  
Vol 3 (7) ◽  
pp. 1-2
Author(s):  
Dr. Gopu Chandran Lenin Perumal ◽  
◽  
Prof. Dr. Mythili .R Prof. Dr. Mythili .R ◽  
Prof. Dr. Senthil Kumar ◽  
Dr. Subashini Suyambukesan
Keyword(s):  
Alkaline Phosphatase ◽  
Chronic Periodontitis ◽  
Total Alkaline Phosphatase ◽  
Total Alkaline ◽  
Phosphatase Enzyme
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