scholarly journals The potency of antagonistic microbes as plant growth-promoting on oil palm seedling infected with basal stem rot disease (Ganoderma boninense.)

2021 ◽  
Vol 782 (4) ◽  
pp. 042060
Author(s):  
F Fadly ◽  
Lisnawita ◽  
I Safni ◽  
K Lubis ◽  
Nurliana
Keyword(s):  
Plant Growth ◽  
Oil Palm ◽  
Stem Rot ◽  
Ganoderma Boninense ◽  
Basal Stem Rot ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rot Disease

scholarly journals Penapisan isolat rizobakteri indigenos untuk pengendalian (Ganoderma boninense) di pre nursery kelapa sawit (Elaeis guineensis Jacq.)

Jurnal Agro ◽  
10.15575/4665 ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 110-122
Author(s):  
Yulmira Yanti ◽  
Imam Rifai ◽  
Yogie Aditya Pratama ◽  
Muhammad Ihsan Harahap
Keyword(s):  
Plant Growth ◽  
Oil Palm ◽  
Control Plant ◽  
Stem Rot ◽  
In Planta ◽  
Basal Stem Rot ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
And Control

Rizobakteri merupakan kelompok bakteri yang aktif mengkolonisasi akar tanaman, meningkatkan pertumbuhan dan mengendalikan patogen tanaman. Penelitian ini bertujuan untuk memperoleh isolat rizobakteri indigenous  terbaik dalam meningkatkan pertumbuhan kelapa sawit dan mengendalikan penyakit busuk pangkal batang di pre-nursery kelapa sawit secara in planta serta karakterisasi kemampuan antagonisnya secara in vitro. Penelitian bersifat eksperimental terdiri atas 3 tahap dengan menggunakan Rancangan Acak Lengkap (RAL): (1) Isolasi dan karakterisasi isolat rizobakteri indigenous  di Kabupaten Pasaman Barat, (2) Pengujian isolat rizobakteri indigenous  (RBI) sebagai plant growth promoting rihzobacteria (PGPR), dan untuk pengendalian G.boninense di pre-nursery kelapa sawit terdiri dari 29 perlakuan (27 isolat RBI, tanpa inokulasi G. boninense sebagai kontrol positif, dan inokulasi G. boninense sebagai kontrol negatif) dengan masing-masing 5 ulangan, serta (3) Pengujian aktivitas antagonisme isolat RBI terhadap G. boninense. Data dianalisis dengan sidik ragam, apabila berbeda nyata dilanjutkan dengan uji Least Significance Different (LSD) pada taraf 5%. Hasil penelitian menunjukkan diperoleh tiga isolat terbaik (R10 2.2, R9 2.1, dan R10 2.3) yang mampu meningkatkan pertumbuhan kelapa sawit dan menekan perkembangan penyakit busuk pangkal batang G.boninense secara in planta dan in vitro.ABSTRACTRhizobacteria is a group of bacteria that actively colonize plant roots, increase growth and control plant pathogen. The objective of the research was to obtain indigenous rhizobacteria isolate (RBI) to increase growth and control basal stem rot on oil palm seedlings in in planta and characterize of antagonistic ability in in vitro. Experimental research consisted of 3 stages by using Completely Randomized Design (CRD): (1) Isolation of indigenous rhizobacteria in West Pasaman region, (2) Indigenous rhizobacteria isolate testing as a plant growth promoting rhizobacteria (PGPR) and to control of G. boninense on pre nursery of oil palm consisted of 29 treatments (27 RBI isolates, without G. boninense inoculation as positive control, and G. boninense inoculation as negative control) with 5 replications each. (3) Testing of RBI isolate antagonism activity towards G. boninense. Data were analyzed by variance, if the result significantly different, it was continued by using Least Significance Different (LSD) at 5% level. The results showed that best three isolates (R10 2.2, R9 2.1 and R10 2.3) were able to increase growth of palm oil and to suppress the development of G.boninense basal stem rot in in planta and in in vitro.

scholarly journals Biocontrol and Plant-Growth-Promoting Traits of Talaromyces apiculatus and Clonostachys rosea Consortium against Ganoderma Basal Stem Rot Disease of Oil Palm

2020 ◽  
Vol 8 (8) ◽  
pp. 1138
Author(s):  
Yit Kheng Goh ◽  
Nurul Fadhilah Marzuki ◽  
Tuan Nur Fatihah Tuan Pa ◽  
Teik-Khiang Goh ◽  
Zeng Seng Kee ◽  
...  
Keyword(s):  
Plant Growth ◽  
Oil Palm ◽  
Biocontrol Agents ◽  
Stem Rot ◽  
Effective Control ◽  
Post Inoculation ◽  
Clonostachys Rosea ◽  
Basal Stem Rot ◽  
Plant Growth Promoting ◽  
Growth Promoting

Basal stem rot (BSR) disease caused by Ganoderma boninense basidiomycetous fungus is the most economically important disease in oil palms in South East Asia. Unfortunately, there is no single most effective control measure available. Tremendous efforts have been directed in incorporation of environmentally friendly biocontrol approaches in minimizing BSR disease. This study investigated the performance of two potential biocontrol agents (BCAs), AAT0115 and AAB0114 strains recovered from oil palm on suppression of BSR in planta, and also assessed their plant-growth-promoting (PGP) performance. ITS rRNA-sequence phylogeny discriminated the two ascomycetous Talaromyces apiculatus (Ta) AT0115 and Clonostachys rosea (Cr) AAB0114 biocontrol species with PGP characteristics. In vitro studies have demonstrated both Ta and Cr are capable of reducing linear mycelial growth of G. boninense. Inoculation of individual Cr and Ta—as well as Cr+Ta consortium—induced a significant increment in leaf area and bole girth of oil-palm seedlings five months post-inoculation (MPI) under nursery conditions. At five months post-inoculation, shoot and root biomass, and nutrient contents (nitrogen, phosphorus, potassium, calcium, magnesium and boron) were significantly higher in Ta-inoculated seedlings compared to control treated with non-Ta-inoculated maize. Chlorophyll and carotenoids contents in rapidly growing oil-palm seedlings challenged with Cr, Ta or a combination of both were not negatively affected. Cr, Ta and Cr+Ta consortium treated seedlings had 4.9–60% BSR disease reduction compared to the untreated control. Co-inoculation of Cr and Ta resulted in increased BSR control efficiencies by 18–26% (compared with Cr only) and 48–55% (compared with Ta only). Collectively, Cr and Ta, either individually or in consortium showed potential as BSR biocontrol agents while also possess PGP traits in oil palm.

scholarly journals Draft Genome Sequence of a Phytopathogenic Ganoderma sp. Strain That Causes Basal Stem Rot Disease on Oil Palm in Sabah, Malaysia

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Christopher L. Y. Voo ◽  
Daniel E. T. Yeo ◽  
Khim-Phin Chong ◽  
Kenneth F. Rodrigues
Keyword(s):  
Genome Sequence ◽  
Oil Palm ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Stem Rot ◽  
Ganoderma Boninense ◽  
Basal Stem Rot ◽  
Content Type ◽  
Rot Disease ◽  
Fungal Genus

Basal stem rot (BSR) disease on Elaeis guineens is known to be caused by members of the pathogenic fungal genus Ganoderma, especially the species Ganoderma boninense. This species affects oil palm plantation in Sabah, Malaysia. The genome sequence (52.28 Mbp) will add to the representation of this genus, especially in regard to BSR disease.

scholarly journals Karakterisasi Bakteri Endofit Kitinolitik sebagai Agens Biokontrol Patogen Ganoderma boninense pada Kelapa Sawit [Characterization of Chitinolytic Endophyte Bacteria as Biocontrol Agents of Ganoderma boninense Pathogen on Oil Palm]

Buletin Palma ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 35
Author(s):  
Rahma Rahma ◽  
Tutik Kuswinanti ◽  
Ade Rosmana
Keyword(s):  
Oil Palm ◽  
Biochemical Characterization ◽  
Stem Rot ◽  
Ganoderma Boninense ◽  
Chitinolytic Activity ◽  
Basal Stem Rot ◽  
Chitinolytic Bacteria ◽  
Biochemical Identification ◽  
Rot Disease

<p>Basal stem rot caused by <em>Ganoderma boninense</em> is one crucial disease in oil palm. The used of chitinolytic bacteria is one part of biological control to suppress <em>G. boninense</em>. This study aimed to isolate and characterize chitinolytic bacteria associated with roots of oil palm which have the potential as agents biocontrol against the pathogens of <em>G. boninense</em>, the caused of basal stem rot disease of oil palm. The study was conducted in North Luwu, East Luwu, and Makassar Districts from January to September 2017. The research was done in  four stages that consist of isolation of endophytic bacterial, testing chitinolytic activity, testing antagonistic and biochemical identification. The results showed that from 14 bacterial isolates obtained, two of them had chitinolytic activity with index of 2,35 and 3,37 respectively. Both of these bacteria can inhibit <em>G. boninense</em> on solid medium by 24,9% and 69,4% respectively and on the liquid medium by 47,5% and 68,5% respectively five days after inoculation. Based on biochemical characterization, these bacteria have similarities with <em>Bacillus</em> sp, and <em>Serratia</em> sp. To be concluded, <em>Serratia</em>sp. is a potential medium to be for controling basal stem rot disease on oil palm.</p><p align="center"><strong>ABSTRAK</strong></p>Busuk pangkal batang yang disebabkan oleh cendawan <em>Ganoderma</em> <em>boninense</em> merupakan salah satu penyakit penting pada kelapa sawit. Pemanfaatan bakteri kitinolitik merupakan salah satu bagian pengendalian hayati untuk mengontrol <em>G. boninense. </em>Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi bakteri kitinolitik yang berasosiasi dengan akar tanaman kelapa sawit yang berpotensi sebagai agens biokontrol terhadap <em>G. boninense, </em>penyebab penyakit busuk pangkal batang kelapa sawit. Penelitian dilaksanakan di Kabupaten Luwu Utara, Luwu Timur, dan Makassar sejak bulan Januari sampai September 2017. Penelitian ini terdiri atas empat tahap, yaitu isolasi bakteri endofit, uji aktivitas kitinolitik, uji antagonis dan identifikasi secara biokimia. Hasil penelitian diperoleh 14 isolat bakteri dimana dua bakteri diantaranya memiliki aktivitas kitinolitik dengan indeks masing masing 2,35 dan 3,37. Kedua bakteri tersebut dapat menghambat <em>G. boninense</em> pada medium padat masing masing 24,9% dan 69,4% serta pada medium cair masing masing 47,5% dan 68,5% lima hari setelah inokulasi. Berdasarkan karakterisasi secara biokimia, bakteri tersebut memiliki kemiripan dengan <em>Bacillus </em>sp, dan <em>Serratia </em>sp. Hasil penelitian menunjukkan bahwa <em>Serratia </em>sp. berpotensi dalam mengendalikan penyakit busuk pangkal batang pada kelapa sawit.<em></em><p> </p><p> </p>

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