Study of rice seed certification at the Gorontalo Province seed certification supervision center

Long-distance transport of the phytohormone abscisic acid (ABA) has been studied for ~50 years, yet its mechanistic basis and biological significance remain very poorly understood. Here, we show that leaf-derived ABA controls rice seed development in a temperature-dependent manner and is regulated by defective grain-filling 1 (DG1), a multidrug and toxic compound extrusion transporter that effluxes ABA at nodes and rachilla. Specifically, ABA is biosynthesized in both WT and dg1 leaves, but only WT caryopses accumulate leaf-derived ABA. Our demonstration that leaf-derived ABA activates starch synthesis genes explains the incompletely filled and floury seed phenotypes in dg1. Both the DG1-mediated long-distance ABA transport efficiency and grain-filling phenotypes are temperature sensitive. Moreover, we extended these mechanistic insights to other cereals by observing similar grain-filling defects in a maize DG1 ortholog mutant. Our study demonstrates that rice uses a leaf-to-caryopsis ABA transport–based mechanism to ensure normal seed development in response to variable temperatures.

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Elucidating ROS signaling networks and physiological changes involved in nanoscale zero valent iron primed rice seed germination sensu stricto

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2021.05.005 ◽

2021 ◽

Author(s):

Titir Guha ◽

Hrimeeka Das ◽

Amitava Mukherjee ◽

Rita Kundu

Keyword(s):

Seed Germination ◽

Sensu Stricto ◽

Zero Valent Iron ◽

Signaling Networks ◽

Rice Seed ◽

Physiological Changes ◽

Ros Signaling ◽

Nanoscale Zero Valent Iron

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Integrated physiological, metabolomic and transcriptomic analyses provide insights into the roles of exogenous melatonin in promoting rice seed germination under salt stress

Plant Growth Regulation ◽

10.1007/s10725-021-00721-9 ◽

2021 ◽

Author(s):

Liexiang Huangfu ◽

Zihui Zhang ◽

Yong Zhou ◽

Enying Zhang ◽

Rujia Chen ◽

...

Keyword(s):

Salt Stress ◽

Seed Germination ◽

Rice Seed ◽

Exogenous Melatonin

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Development of Material Combination Model Considering Economics and Construction Efficiency for G-SEED Certification

Sustainability ◽

10.3390/su13063535 ◽

2021 ◽

Vol 13 (6) ◽

pp. 3535

Author(s):

Byung-Ju Jeon ◽

Byung-Soo Kim

Keyword(s):

Structural Design ◽

Material Selection ◽

Selection Model ◽

Construction Costs ◽

Seed Certification ◽

Evaluation Scores ◽

The Cost ◽

Material Information ◽

Business As Usual ◽

Construction Efficiency

The Korean government proposed a goal to reduce its greenhouse gas emissions by 37% compared to business-as-usual levels by 2030 and launched the Green Standard for Energy and Environmental Design (G-SEED) certification system. The certification requires meeting the required score and material selection with a secured economy and construction efficiency. However, most buildings only focus on obtaining the certification scores instead of choosing economical materials with high construction efficiency. This research focused on developing a material selection model that considers both the construction efficiency and economy of the materials and the acquisition of material and resource evaluation scores from the G-SEED certification. This research, therefore, analyzed actual data to automate the material selection and compare alternatives to using a genetic algorithm to obtain optimized alternatives. This model proposes an alternative to constructability and economy when the required score and material information is entered. When the model was applied to actual cases, the result revealed a reduction in construction costs of about 37% compared to the cost with the traditional methods. The material selection model from this research can benefit construction project owners in terms of cost reduction, designers in terms of structural design time, and constructors in terms of construction efficiency

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LAMP-based foldable microdevice platform for the rapid detection of Magnaporthe oryzae and Sarocladium oryzae in rice seed

Scientific Reports ◽

10.1038/s41598-020-80644-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

M. K. Prasannakumar ◽

P. Buela Parivallal ◽

Devanna Pramesh ◽

H. B. Mahesh ◽

Edwin Raj

Keyword(s):

Magnaporthe Oryzae ◽

Plant Pathogens ◽

Point Of Care ◽

Lamp Assay ◽

Rice Seed ◽

Highly Sensitive ◽

Polymerase Chain ◽

Rice Seeds ◽

Template Dna ◽

Assay Conditions

AbstractRice blast (caused by Magnaporthe oryzae) and sheath rot diseases (caused by Sarocladium oryzae) are the most predominant seed-borne pathogens of rice. The detection of both pathogens in rice seed is essential to avoid production losses. In the present study, a microdevice platform was designed, which works on the principles of loop-mediated isothermal amplification (LAMP) to detect M. oryzae and S. oryzae in rice seeds. Initially, a LAMP, polymerase chain reaction (PCR), quantitative PCR (qPCR), and helicase dependent amplification (HDA) assays were developed with primers, specifically targeting M. oryzae and S. oryzae genome. The LAMP assay was highly efficient and could detect the presence of M. oryzae and S. oryzae genome at a concentration down to 100 fg within 20 min at 60 °C. Further, the sensitivity of the LAMP, HDA, PCR, and qPCR assays were compared wherein; the LAMP assay was highly sensitive up to 100 fg of template DNA. Using the optimized LAMP assay conditions, a portable foldable microdevice platform was developed to detect M. oryzae and S. oryzae in rice seeds. The foldable microdevice assay was similar to that of conventional LAMP assay with respect to its sensitivity (up to 100 fg), rapidity (30 min), and specificity. This platform could serve as a prototype for developing on-field diagnostic kits to be used at the point of care centers for the rapid diagnosis of M. oryzae and S. oryzae in rice seeds. This is the first study to report a LAMP-based foldable microdevice platform to detect any plant pathogens.

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