scholarly journals In vitro selection of birch for tolerance to salinity stress

2021 ◽  
Vol 875 (1) ◽  
pp. 012082
Author(s):  
O S Mashkina ◽  
T M Tabatskaya ◽  
O M Korchagin
Keyword(s):  
Betula Pendula ◽  
In Vitro Selection ◽  
Callus Formation ◽  
Callus Cultures ◽  
Cellular Heterogeneity ◽  
Stem Explants ◽  
Salt Tolerant ◽  
Tolerance To Salinity ◽  
Selection Of

Abstract In vitro modelling of stress is one of the promising avenues for plant breeding for tolerance to negative environmental factors. In this study we examined the effect of NaCl (0.5%) on callusogenesis and morphogenesis of stem explants of different birch genotypes: Betula pendula Roth, B. pendula Roth var. carelica (Mercklin) Hämet-Ahti, B. pendula f. ‘dalecarlica’ (L.f.) Schneid., B. pubescens Ehrh. In our experiments we used pre-selected microclones from our in vitro collection on NaCl (0.2-1.0%) selective media. The clones were contrasted by the degree of their sensitivity to salinity (so-called ‘stable’ and ‘sensitive’ microclones). With the use of stem callus cultures we identified informative, simple and reproducible indicators for the selection of salt-tolerant genotypes. Among these indicators were the frequency of callus formation and the viability of callus cultures, which were significantly higher in ‘stable’ group of microclones. Polyploid birch clones (2n=4x=56, 2n=3x=42) were more resistant to salination compared to diploid clones (2n=28). Our study has shown that the selection of salt-tolerant birch lines can be based on the plants’ genetic diversity presented in the collection (various species, varieties, hybrids, polyploids) and manifested in the process of in vitro cultivation, as well as in the cellular heterogeneity of callus cultures.

scholarly journals Rapid in vitro selection of salt-tolerant genotypes of the potentially medicinal plant Centaurium maritimum (L.) fritsch

2009 ◽  
Vol 61 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Danijela Misic ◽  
B. Siler ◽  
Biljana Filipovic ◽  
Zorica Popovic ◽  
Suzana Zivkovic ◽  
...  
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Salinity Tolerance ◽  
Growth Phase ◽  
In Vitro Selection ◽  
Growth Phases ◽  
Salt Tolerant ◽  
Variable Response ◽  
Selection Of

We investigated differences of salinity tolerance between 'salt-tolerant' (ST) and 'salt-sensitive' (SS) genotypes of yellow centaury [Centaurium maritimum (L.) Fritsch] selected during the germination phase. The ability of in vitro cultured C. maritimum to complete the whole ontogenetic cycle in less than 6 months enabled us to deterine salinity tolerance during different growth phases. Based on the physiological attributes measured in this study (growth, morphogenesis, photosynthesis, flowering, seed germination), it can be concluded that C. maritimum genotypes differing in salinity tolerance showed a variable response to elevated salt concentrations during both the vegetative and the generative growth phase.

In vitro selection of salt-tolerant cell lines in kallar grass [Diplachne fusca (L.) Beauv.]

2003 ◽  
Vol 3 (1) ◽  
pp. 49-52 ◽  
Author(s):  
MALEE NANAKORN ◽  
WALAIKARN JIAMJETJAROON ◽  
SRISOM SUWANAWONG ◽  
CHALERMCHAI WONGWATTANA ◽  
IE SUNG SHIM
Keyword(s):  
Cell Lines ◽  
In Vitro Selection ◽  
Salt Tolerant ◽  
Kallar Grass ◽  
Tolerant Cell ◽  
Selection Of

In vitro selection of salt tolerant cell lines in Solanum tuberosum L.

2007 ◽  
Vol 51 (4) ◽  
pp. 728-734 ◽  
Author(s):  
F. Queiros ◽  
F. Fidalgo ◽  
I. Santos ◽  
R. Salema
Keyword(s):  
Solanum Tuberosum ◽  
Cell Lines ◽  
In Vitro Selection ◽  
Solanum Tuberosum L ◽  
Salt Tolerant ◽  
Tolerant Cell ◽  
Selection Of

IN VITRO SELECTION OF SALT TOLERANT ARTICHOKE HYBRIDS

2011 ◽  
pp. 177-181
Author(s):  
A. Campanelli ◽  
C. Ruta ◽  
N. Calabrese ◽  
I. Morone-Fortunato
Keyword(s):  
In Vitro Selection ◽  
Salt Tolerant ◽  
Selection Of

scholarly journals IN VITRO SELECTION OF SALT-TOLERANT OLIVE CLONES

2008 ◽  
pp. 217-223 ◽  
Author(s):  
J. Troncoso ◽  
J. Liñán ◽  
M. Cantos ◽  
J.L. García ◽  
A. Troncoso
Keyword(s):  
In Vitro Selection ◽  
Salt Tolerant ◽  
Selection Of

In vitro selection of glyphosate-tolerant variants from long-term callus cultures of Zoysia matrella [L.] Merr

2012 ◽  
Vol 111 (2) ◽  
pp. 199-207 ◽  
Author(s):  
Shu Chen ◽  
Mingliang Chai ◽  
Yufang Jia ◽  
Zhongshan Gao ◽  
Li Zhang ◽  
...  
Keyword(s):  
In Vitro Selection ◽  
Callus Cultures ◽  
Zoysia Matrella ◽  
Selection Of

scholarly journals Microclonal propagation and callus induction of some species of Carlina L. genus

2018 ◽  
Vol 22 ◽  
pp. 274-281
Author(s):  
N. B. Kravets ◽  
N. V. Tulaidan ◽  
M. Z. Mosula ◽  
N. M. Drobyk
Keyword(s):  
Callus Induction ◽  
Callus Tissue ◽  
Callus Formation ◽  
Callus Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Semi Solid ◽  
Indole 3 Acetic Acid

Aim. The aim of the research was to choose the conditions for microclonal propagation and obtain callus cultures from Carlina аcaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl plants in vitro. Methods. For microclonal propagation of С. acaulis, C. cirsioides and C. onopordіfolia we used rosettes of 2–3-month specimens and planted them on semi-solid Murashige and Skoog (MS) medium with decreased macro- and microsalts concentrations (MS/2) supplemented with kinetin (Кin) (from 1–3 mg/l) and 0.1 mg/l of 1-naphthaleneacetic acid (NAA). For induction of callus formation, we used root, stem explants from С. acaulis, C. cirsioides and C. onopordіfolia, and planted them on nutrient media MS, MS/2, and Gamborg and Eveleigh (В5) supplemented with different concentrations of cytokinins – 6-benzylaminopurine (BAP) or Кin and auxins – 2.4-dichlorophenoxyacetic acid (2.4-D) or NAA and indole-3-acetic acid (IAA). Results. MS/2 medium supplemented with growth regulators of NAA and Кin were the most efficient to provide the formation of microclones. For C. сirsioides plants, this indicator was 6.6–6.8 rosettes per graft after 6 months of cultivation and for С. acaulis and C. onopordіfolia – 4.2–5.0 and 4.8–5.2 respectively. To raise the percentage of rooting for microclones of Carlina species, it was expedient to steep them preliminarily in the solution of indole-3-butyric acid (IBA) with 1000 mg/l concentration for a minute. Optimal for obtaining callus tissue from Carlina plants was nutrient medium MS supplemented with 3 mg/l IAA, 0.5 mg/l NAA and 0.5 mg/l Kin and MS/2 with 0.1 mg/l BAP and 0.5 mg/l 2.4-D; under such conditions the percentage of callus induction exceeded 90 % for all types of explants. Conclusions. There were chosen the conditions for microclonal propagation of С. acaulis, C. cirsioides and C. onopordіfolia and worked out the schemes for enrooting obtained microclones in vitro. Capable of growing rapidly callus cultures from root and stem explants of the investigated plant species were obtained. Keywords: Carlina аcaulis L., Carlina cirsioides  Klok, Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl, in vitro, microclonal propagation, callus induction.

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