scholarly journals Assisted reproductive technology in tropical animals: Case in Pasundan cattle genetic conservation and utilization

2021 ◽  
Vol 902 (1) ◽  
pp. 012036
Author(s):  
R Widyastuti ◽  
D N Pristihadi ◽  
S Prastowo ◽  
H Maheshwari ◽  
C Sumantri ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Assisted Reproductive Technology ◽  
Artificial Insemination ◽  
Genetic Material ◽  
Reproductive Technology ◽  
Sex Selection ◽  
Advanced Technology ◽  
Sperm Cryopreservation ◽  
Embryo Production

Abstract Pasundan cattle are native Indonesian cattle located in West Java, Indonesia. These cattle have a superior trait in reproductive performance although reared in extreme environmental conditions with low-quality of feed, and resistance to parasite diseases than other cattle breeds. However, the ability to maintain Pasundan cattle genetic diversity, avoiding inbreeding, keeping the population healthy, and high reproduction efficiency are among the significant challenges currently occurred. The application of assisted reproductive technology (ART) seems promising to overcome those challenges and would impact the efficiency of genetic diversity conservation and preservation. This review aims to present the possibility to use the available ART in Pasundan cattle. The ART reviewed are the advanced technology of sperm cryopreservation, sex selection, artificial insemination, and in vitro embryo production. The sperm cryopreservation combined with sperm sexing is the most promising technology to maximize the use of genetic material of Pasundan cattle. Moreover, sexed sperm would have a promising impact on artificial insemination and/or embryo production to increase the cattle population. Furthermore, to protect genetic diversity, it is possible to apply the artificial reproductive technique and sperm or embryos conservation for genetic utilization in the future.

scholarly journals Assisted Reproductive Technology in Neotropical Deer: A Model Approach to Preserving Genetic Diversity

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1961
Author(s):  
Luciana Diniz Rola ◽  
Marcos Eli Buzanskas ◽  
Luciana Magalhães Melo ◽  
Maiana Silva Chaves ◽  
Vicente José Figueirêdo Freitas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Assisted Reproductive Technology ◽  
Wildlife Conservation ◽  
Reproductive Technology ◽  
Embryo Production ◽  
Cell Banks ◽  
Ovarian Follicular Fluid ◽  
Neotropical Deer

One of the most significant challenges in deer is the ability to maintain genetic diversity, avoiding inbreeding and sustaining population health and reproduction. Although our general knowledge of reproductive physiology is improving, it appears that the application of assisted reproductive technology (ART) will more efficiently advance wildlife conservation efforts and preserve genetic diversity. The purpose of this review is to present the most important results obtained with the use of ART in Neotropical deer. Thus, the state-of-the-art for estrus synchronization, semen technology, artificial insemination, and in vivo embryo production will be presented. In vitro embryo production (IVP) is also a biotechnology that is taking initial steps in deer. In this aspect, the approach with the proteomics of ovarian follicular fluid is being used as a tool for a better understanding of oocyte maturation. Finally, cell banks and the use of interspecific somatic cell nuclear transfer (iSCNT) as well as the use of stem cells for gametes differentiation are promising techniques.

A comprehensive method for the conservation of mouse strains combining natural breeding, sperm cryopreservation and assisted reproductive technology

Zygote ◽  
2012 ◽  
Vol 22 (2) ◽  
pp. 132-137 ◽  
Author(s):  
Dun-Gao Li ◽  
Yan Zhu ◽  
He-Ping Li ◽  
Xue-Jin Chen ◽  
Man-Xi Jiang
Keyword(s):  
Assisted Reproductive Technology ◽  
Reproductive Technology ◽  
Mouse Strains ◽  
Epididymal Sperm ◽  
Sperm Cryopreservation ◽  
Unique Challenge ◽  
Comprehensive Method ◽  
Cryopreserved Sperm ◽  
Natural Breeding

SummaryThe maintenance and preservation of strains of mice used in biomedical research presents a unique challenge to individual investigators and research institutions. The goal of this study was to assess a comprehensive system for mouse strain conservation through a combination of natural mating, sperm cryopreservation and assisted reproductive technology. Our strategy was based on the collection and cryopreservation of fresh epididymal sperm from male mice by semi-vasectomy; these mice were then naturally mated for breeding purposes. If no satisfactory results were obtained from natural breeding, then the cryopreserved sperm were used for in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI); resultant embryos were then transferred into pseudopregnant-recipient female mice. Our results show that some semi-vasectomized mouse strains can be conserved by natural breeding, and that sterile males can be compensated for through the use of IVF and ICSI technology. As such, we believe this system is suitable for the purpose of strain conservation, allowing the continuation of natural breeding with the safeguard of assisted reproduction available.

Follicular fluid and assisted reproductive technology programs outcomes (literature review)

GYNECOLOGY ◽  
2020 ◽  
Vol 21 (6) ◽  
pp. 36-40
Author(s):  
Anna G. Burduli ◽  
Natalia A. Kitsilovskaya ◽  
Yuliya V. Sukhova ◽  
Irina A. Vedikhina ◽  
Tatiana Y. Ivanets ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Assisted Reproductive Technology ◽  
Follicular Fluid ◽  
Economic Cost ◽  
Reproductive Technology ◽  
Art Programs ◽  
Vitro Fertilization ◽  
Non Invasive ◽  
Established Fact

The review presents data on metabolites in the follicular fluid (FF) from the perspective of reproductive medicine and their use in order to predict outcomes of assisted reproductive technology (ART) programs. It considers various components of this biological medium (hormones, lipids, melatonin, etc.) with an assessment of their predictive value in prognosis of the effectiveness of in vitro fertilization (IVF) programs. The data on experimental directions in this field and the prospects for their use in clinical practice are presented. The article emphasizes that the growing clinical need and the unsolved problem of increasing the effectiveness of ART programs determine the need for further studies of the FF composition. Materials and methods. The review includes data related to this topic from foreign and Russian articles found in PubMed which were published in recent years. Results. Given the established fact of a direct effect of FF composition on growth and maturation of oocytes, and further, on the fertilization process, various FF metabolites are actively investigated as non-invasive markers of quality of oocytes/embryos. The article provides data on the experimental directions in this field and the prospects for their use in clinical practice. However, clinical studies of a relation between various FF metabolites levels and outcomes of IVF programs are contradictory. Conclusion. Owing large economic cost for treatment of infertility with IVF, there is need for expansion and intensification of studies to identify and use reliable predictors in prognosis of ART programs outcomes.

Application of reproductive technology to the Australian livestock industries

1991 ◽  
Vol 3 (6) ◽  
pp. 627 ◽  
Author(s):  
G Evans
Keyword(s):  
Artificial Insemination ◽  
Management Practices ◽  
Significant Degree ◽  
Reproductive Technology ◽  
Commercial Application ◽  
Embryo Freezing ◽  
Commercial Practice ◽  
Breeding Programmes ◽  
The Impact

Current use of reproductive technology in the Australian livestock industries is limited, though it increased in line with higher prices for beef and wool through the 1980s. The required techniques, many of which were developed in Australia, are available and the level of expertise is comparable to the best in the world. However, the extensive pastoral industries do not readily lend themselves to these procedures. Only in the dairy industry is artificial insemination used to a significant degree. On the other hand, application of the technology in the pastoral industries is confined largely to studs and breeding cooperatives which provide breeding animals for producer flocks and herds. Hence the impact of applied technology may be more widespread than first appears. Until recently, little regard was paid to application of the technology along sound breeding principles. Artificial insemination and multiple ovulation and embryo transfer (MOET) have not been used so much in planned breeding programmes aimed at local improvement of stock, but more to proliferate genes of reputedly superior stock, imported either from overseas or elsewhere in Australia. This is particularly true of MOET, where the incentive to use it is commonly a short term cash gain made from proliferating breeding stock of a particularly valuable and usually novel strain or breed. Recent technological improvements which render the use of reproductive technology cheaper and more effective will lead to its more widespread use in commercial practice. Techniques for embryo freezing and splitting have been greatly simplified and quickly put into practice. The novel livestock technologies of in vitro oocyte maturation and fertilization have already found commercial application overseas. Fecundity-enhancing products have also been adopted by the livestock industries. There is potential value for greater use of reproductive technology in the livestock industries provided it is implemented according to sound breeding principles and provided associated management practices are applied simultaneously.

Pregnancy Outcomes Using Assisted Reproductive Technology After Fertility-Preserving Therapy in Patients With Endometrial Adenocarcinoma or Atypical Complex Hyperplasia

2009 ◽  
Vol 19 (1) ◽  
pp. 147-151 ◽  
Author(s):  
Aera R. Han ◽  
Yong-Soon Kwon ◽  
D. Y. Kim ◽  
J. H. Kim ◽  
Y. M. Kim ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Assisted Reproductive Technology ◽  
Intrauterine Insemination ◽  
Medical Center ◽  
Endometrial Adenocarcinoma ◽  
Controlled Ovarian Hyperstimulation ◽  
Reproductive Technology ◽  
Vitro Fertilization ◽  
Asan Medical

Objectives:To evaluate the outcomes of pregnancy in young women (<40 years old) with early endometrial cancer or atypical complex hyperplasia who were treated by conservative management followed by assisted reproductive technology (ART).Materials and Methods:Medical charts of 11 patients treated from January 1997 to October 2007 at Asan Medical Center were retrospectively reviewed. These patients had all been treated with progestin and serial dilatation and curettage as primary fertility-preserving therapies.Results:After pathological remission of disease, 10 patients tried to become pregnant by ART, 4 by in vitro fertilization and embryo transfer, and 6 by controlled ovarian hyperstimulation, with or without intrauterine insemination. Eight women had intrauterine pregnancies, and 6 patients had live births. Patients have been followed up for 9 to 51 months (mean, 21 months) after delivery, with no evidence of tumor recurrence.Conclusions:Fertility-preserving therapy followed by ART can be a good option in well-selected patients with early endometrial cancer who want to become pregnant.

In Vitro Fertilization, Assisted Reproductive Technology

2013 ◽  
pp. 1050-1052
Author(s):  
Marie Boltz ◽  
Holly Rau ◽  
Paula Williams ◽  
Holly Rau ◽  
Paula Williams ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Assisted Reproductive Technology ◽  
Reproductive Technology ◽  
Vitro Fertilization
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