scholarly journals Assisted Reproductive Technology in Neotropical Deer: A Model Approach to Preserving Genetic Diversity

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1961
Author(s):  
Luciana Diniz Rola ◽  
Marcos Eli Buzanskas ◽  
Luciana Magalhães Melo ◽  
Maiana Silva Chaves ◽  
Vicente José Figueirêdo Freitas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Assisted Reproductive Technology ◽  
Wildlife Conservation ◽  
Reproductive Technology ◽  
Embryo Production ◽  
Cell Banks ◽  
Ovarian Follicular Fluid ◽  
Neotropical Deer

One of the most significant challenges in deer is the ability to maintain genetic diversity, avoiding inbreeding and sustaining population health and reproduction. Although our general knowledge of reproductive physiology is improving, it appears that the application of assisted reproductive technology (ART) will more efficiently advance wildlife conservation efforts and preserve genetic diversity. The purpose of this review is to present the most important results obtained with the use of ART in Neotropical deer. Thus, the state-of-the-art for estrus synchronization, semen technology, artificial insemination, and in vivo embryo production will be presented. In vitro embryo production (IVP) is also a biotechnology that is taking initial steps in deer. In this aspect, the approach with the proteomics of ovarian follicular fluid is being used as a tool for a better understanding of oocyte maturation. Finally, cell banks and the use of interspecific somatic cell nuclear transfer (iSCNT) as well as the use of stem cells for gametes differentiation are promising techniques.

scholarly journals Assisted reproductive technology in tropical animals: Case in Pasundan cattle genetic conservation and utilization

2021 ◽  
Vol 902 (1) ◽  
pp. 012036
Author(s):  
R Widyastuti ◽  
D N Pristihadi ◽  
S Prastowo ◽  
H Maheshwari ◽  
C Sumantri ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Assisted Reproductive Technology ◽  
Artificial Insemination ◽  
Genetic Material ◽  
Reproductive Technology ◽  
Sex Selection ◽  
Advanced Technology ◽  
Sperm Cryopreservation ◽  
Embryo Production

Abstract Pasundan cattle are native Indonesian cattle located in West Java, Indonesia. These cattle have a superior trait in reproductive performance although reared in extreme environmental conditions with low-quality of feed, and resistance to parasite diseases than other cattle breeds. However, the ability to maintain Pasundan cattle genetic diversity, avoiding inbreeding, keeping the population healthy, and high reproduction efficiency are among the significant challenges currently occurred. The application of assisted reproductive technology (ART) seems promising to overcome those challenges and would impact the efficiency of genetic diversity conservation and preservation. This review aims to present the possibility to use the available ART in Pasundan cattle. The ART reviewed are the advanced technology of sperm cryopreservation, sex selection, artificial insemination, and in vitro embryo production. The sperm cryopreservation combined with sperm sexing is the most promising technology to maximize the use of genetic material of Pasundan cattle. Moreover, sexed sperm would have a promising impact on artificial insemination and/or embryo production to increase the cattle population. Furthermore, to protect genetic diversity, it is possible to apply the artificial reproductive technique and sperm or embryos conservation for genetic utilization in the future.

scholarly journals Association of Assisted Reproductive Technology Treatments with Imprinting Disorders

2021 ◽  
Author(s):  
T. Kopca ◽  
Pinar Tulay
Keyword(s):  
Assisted Reproductive Technology ◽  
Case Reports ◽  
Reproductive Technology ◽  
Preimplantation Embryos ◽  
Imprinting Disorders ◽  
Couples Treatment ◽  
Silver Russell Syndrome ◽  
Gene Expression Levels

AbstractAssisted reproductive technology (ART) is a broad field in infertility that encompasses different types of treatments. These revolutionary treatment methods aimed to aid infertile or subfertile couples. Treatment was expanded exponentially, as 1 to 3% of the births worldwide takes place with ART procedures. However, treatment is not flawless. Gametes and embryos are exposed to different chemicals and stress through treatment, which leads to disturbance in proper embryo development and results in prenatal and congenital anomalies. When compared with in-vivo development of gametes and preimplantation embryos in mice, in-vitro conditions during ART treatments have been suggested to disturb the gene expression levels, especially imprinted genes. Therefore, ART has been suggested to be associated with increased incidences of different imprinting disorders such as Beckwith–Wiedemann syndrome, Angelman syndrome, and Silver–Russell syndrome, as proved by different case reports and studies. This literature review aims to explain the association of imprinting disorders with this revolutionary treatment procedure.

The role of animal studies in supporting human assisted reproductive technology

2004 ◽  
Vol 16 (7) ◽  
pp. 719 ◽  
Author(s):  
Barry Bavister
Keyword(s):  
Assisted Reproductive Technology ◽  
Animal Studies ◽  
Reproductive Technology ◽  
Human Embryos ◽  
Success Rates ◽  
Animal Art

Although average success rates of human IVF have increased progressively during the past two decades, the efficiency of this technique, based on each embryo produced or transferred, is still low. High success rates are usually achieved by transferring several embryos to the patient, which is often associated with multiple pregnancies. The quality of in vitro produced embryos is a major area that needs attention. Because there is no in vivo database for human embryos, the properties of normal embryos are not known, and so it is difficult to know how to improve quality and viability. In addition, selection of the most viable embryos for transfer is a rather subjective process. The origins of human assisted reproductive technology (ART) are based on animal ART; however, the two areas of research (animal and human ART) appear to have become disconnected. Re-examination of progress in animal ART could help improve human embryo quality and thereby assist efforts to sustain high pregnancy rates with only one or two embryos transferred. Some key areas in which animal ART can help guide progress in human ART are discussed.

Oviductal cells and early conception

1995 ◽  
Vol 4 (1) ◽  
pp. 31-41 ◽  
Author(s):  
Ariff Bongso
Keyword(s):  
Assisted Reproductive Technology ◽  
Fallopian Tube ◽  
Functional Significance ◽  
Reproductive Technology ◽  
Embryo Viability ◽  
Uterine Environment ◽  
Functional Relevance

The ability to bypass the oviduct by fertilizing human oocytes in vitro and then transferring the ensuing embryos into the uterine environment to produce pregnancies that can be carried to term (assisted reproductive technology, ART) has made the real functional significance of the fallopian tube come under debate. The question has been raised as to whether the oviduct is a simple conduit, providing for the transport of gametes and embryos, or whether it has any functional relevance to early conception. In the field of ART, some reports claim that transfer of embryos either to the uterus or to the oviduct yield similar results, while a greater amount of evidence confirms that tubal transfers are associated with higher pregnancy and take home baby rates, suggesting that unknown factors in the oviduct favour fertilization and embryo viability. This review attempts to provide some answers to questions about the role of oviductal cells in vivo during early conception and in vitro in the field of ART.

Follicular fluid and assisted reproductive technology programs outcomes (literature review)

GYNECOLOGY ◽  
2020 ◽  
Vol 21 (6) ◽  
pp. 36-40
Author(s):  
Anna G. Burduli ◽  
Natalia A. Kitsilovskaya ◽  
Yuliya V. Sukhova ◽  
Irina A. Vedikhina ◽  
Tatiana Y. Ivanets ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Assisted Reproductive Technology ◽  
Follicular Fluid ◽  
Economic Cost ◽  
Reproductive Technology ◽  
Art Programs ◽  
Vitro Fertilization ◽  
Non Invasive ◽  
Established Fact

The review presents data on metabolites in the follicular fluid (FF) from the perspective of reproductive medicine and their use in order to predict outcomes of assisted reproductive technology (ART) programs. It considers various components of this biological medium (hormones, lipids, melatonin, etc.) with an assessment of their predictive value in prognosis of the effectiveness of in vitro fertilization (IVF) programs. The data on experimental directions in this field and the prospects for their use in clinical practice are presented. The article emphasizes that the growing clinical need and the unsolved problem of increasing the effectiveness of ART programs determine the need for further studies of the FF composition. Materials and methods. The review includes data related to this topic from foreign and Russian articles found in PubMed which were published in recent years. Results. Given the established fact of a direct effect of FF composition on growth and maturation of oocytes, and further, on the fertilization process, various FF metabolites are actively investigated as non-invasive markers of quality of oocytes/embryos. The article provides data on the experimental directions in this field and the prospects for their use in clinical practice. However, clinical studies of a relation between various FF metabolites levels and outcomes of IVF programs are contradictory. Conclusion. Owing large economic cost for treatment of infertility with IVF, there is need for expansion and intensification of studies to identify and use reliable predictors in prognosis of ART programs outcomes.

Pregnancy Outcomes Using Assisted Reproductive Technology After Fertility-Preserving Therapy in Patients With Endometrial Adenocarcinoma or Atypical Complex Hyperplasia

2009 ◽  
Vol 19 (1) ◽  
pp. 147-151 ◽  
Author(s):  
Aera R. Han ◽  
Yong-Soon Kwon ◽  
D. Y. Kim ◽  
J. H. Kim ◽  
Y. M. Kim ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Assisted Reproductive Technology ◽  
Intrauterine Insemination ◽  
Medical Center ◽  
Endometrial Adenocarcinoma ◽  
Controlled Ovarian Hyperstimulation ◽  
Reproductive Technology ◽  
Vitro Fertilization ◽  
Asan Medical

Objectives:To evaluate the outcomes of pregnancy in young women (<40 years old) with early endometrial cancer or atypical complex hyperplasia who were treated by conservative management followed by assisted reproductive technology (ART).Materials and Methods:Medical charts of 11 patients treated from January 1997 to October 2007 at Asan Medical Center were retrospectively reviewed. These patients had all been treated with progestin and serial dilatation and curettage as primary fertility-preserving therapies.Results:After pathological remission of disease, 10 patients tried to become pregnant by ART, 4 by in vitro fertilization and embryo transfer, and 6 by controlled ovarian hyperstimulation, with or without intrauterine insemination. Eight women had intrauterine pregnancies, and 6 patients had live births. Patients have been followed up for 9 to 51 months (mean, 21 months) after delivery, with no evidence of tumor recurrence.Conclusions:Fertility-preserving therapy followed by ART can be a good option in well-selected patients with early endometrial cancer who want to become pregnant.

In Vitro Fertilization, Assisted Reproductive Technology

2013 ◽  
pp. 1050-1052
Author(s):  
Marie Boltz ◽  
Holly Rau ◽  
Paula Williams ◽  
Holly Rau ◽  
Paula Williams ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Assisted Reproductive Technology ◽  
Reproductive Technology ◽  
Vitro Fertilization

scholarly journals Significance of disorders of proteomic profile of follicular fluid for predicting the effectiveness of in vitro fertilisation in women with infertility

2018 ◽  
Vol 99 (3) ◽  
pp. 496-503
Author(s):  
O S Zolotykh ◽  
S V Lomteva ◽  
K Yu Sagamonova
Keyword(s):  
Assisted Reproductive Technology ◽  
Follicular Fluid ◽  
Molecular Mechanisms ◽  
Reproductive Technologies ◽  
Reproductive Technology ◽  
In Vitro Fertilisation ◽  
Proteomic Profile ◽  
Technology Programs ◽  
Group 2

Aim. To study the proteomic profile of follicular fluid in patients with infertility in assisted reproductive technology programs. Methods. The study included women with infertility included in assisted reproductive technology programs: 15 women who had in vitro fertilisation which resulted in pregnancy (group 1) and 16 women with a negative result of this program (group 2). Fractionation of the follicular fluid samples was performed using the sets of special magnetic beads. Proteomic profiling was performed by tandem MALDI-mass-spectrometry. The anti-Müllerian hormone level was measured by ELISA. Results. The study revealed differences in the detectability of follicular fluid proteins with different regulatory properties in patients of groups 1 and 2. With the negative outcome of in vitro fertilisation, expression of a number of proteins involved in the processes of folliculogenesis, ovulation, selection of the dominant follicle, as well as proteins necessary for the development of the zygote and blastula was reduced in females' follicular fluid. Increased expression in women from group 2 was registered for proteins enhancing proteolytic reactions, cell apoptosis, including oocytes, which disrupt the positive action of activin and damage structural and functional state of mitochondria. A definite relationship was found between the level of anti-Müllerian hormone and rate of detection of a number of proteins, in particular protocadherin-2α, cystatin C, betaglycan, prostatic acid phosphatase, and dermicidin. Conclusion. The revealed changes in proteomic profile of the follicular fluid obviously play an important role in the molecular mechanisms that determine the effectiveness of assisted reproductive technologies; the identified differentially expressed proteins can serve as objective markers for predicting the outcomes of in vitro fertilisation.

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