scholarly journals Genetic diversity based on RAPD F2 generation of apple cactus (Cereus spp.)

2021 ◽  
Vol 905 (1) ◽  
pp. 012073
Author(s):  
D E Anindyaswari ◽  
Sukaya ◽  
E S Muliawati ◽  
D W Djoar
Keyword(s):  
Genetic Diversity ◽  
Dna Isolation ◽  
Simple Matching Coefficient ◽  
F2 Generation

Abstract Apple cactus consists of 2 types, long spines (Cereus jamacaru) and short spines (C. peruvianus); both are self-incompatible. So that only artificial crosses between species that produce fruit. But some fruits are found from natural crosses of long spines. The three results of these crosses, long spines with short spines; short spines with long spines; and natural crosses on long spines, have produced F2 seedlings. The purpose of this study was to study genetic diversity based on the RAPD of the F2 seedlings. DNA isolation was carried out using Doyle and Doyle method modified on the addition of Polivinilpirolidon. The RAPD technique uses 3 primers: OPD-11, OPM-10, and OPN-5. Analysis of genetic diversity using Simple Matching coefficient using NTSys 2.02 software. Based on the three primers, each F2 offspring had genetic diversity but was grouped according to the parent. Showed that the three RAPD primers were effective for markers of genetic diversity in apple cactus.

scholarly journals Keanekaragaman Genetik Nyamuk Vektor Filariasis Culex quinquefasciatus Say, 1823 (Diptera: Culicidae) di Kota dan Kabupaten Pekalongan Dengan Metode PCR-RAPD

2018 ◽  
Vol 1 (2) ◽  
pp. 42
Author(s):  
Anindita Riesti Retno Arimurti
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Culex Quinquefasciatus ◽  
Genetic Characterization ◽  
Wuchereria Bancrofti ◽  
High Genetic Diversity ◽  
Simple Matching Coefficient

Mosquito Culex quinquefasciatus is a vector of nematode worms, namely Wuchereria bancrofti which is the cause of filariasis in tropical and subtropical countries. Distributed of Cx. quinquefasciatus is widely in Indonesia with differences the geographical, resulting in the adaptation to the environment and may  results in a high variation, both phenotypic (morphology) and genotypic (genetic) variation. This study aims was to determine the genetic diversity of mosquitoes Cx.quinquefasciatus as vector filariasis in Pekalongan City and Regent. Genetic characterization performed by PCR-RAPD using three primers, ie OPA-11, OPA-12, and OPA-15. Data were analyzed by using UPGMA algorithm and Simple Matching Coefficient and presented as dendrogram. The results showed a high genetic diversity with the polymorphisms up to 100%.  Keywords: Culex quinquefasciatus, vector, filariasis, PCR-RAPD 

Optimization of DNA isolation and PCR protocol for analysis and evaluation of genetic diversity of the medicinal plant,Anemopsis californicausing RAPD

2013 ◽  
Vol 64 (2) ◽  
pp. 184-195 ◽  
Author(s):  
C. Lizette Del-Toro-Sánchez ◽  
S. Villaseñor-Alvarado ◽  
Florentina Zurita-Martínez ◽  
O. Castellanos-Hernández ◽  
Araceli Rodríguez-Sahagún ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Medicinal Plant ◽  
Dna Isolation ◽  
Analysis And Evaluation

scholarly journals OPTIMASI SUHU ANNEALING UNTUK PRIMER g-SSR DAN EST-SSR PADA KACANG HIJAU (Vigna radiata L.)

2019 ◽  
Vol 33 (1) ◽  
pp. 95-102
Author(s):  
Herman Herman ◽  
Lambok Nia Natalya ◽  
Suha Maudina Berampu ◽  
Dewi Indriyani Roslim
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Vigna Radiata ◽  
Genetic Information ◽  
Dna Isolation ◽  
Dna Amplification ◽  
Accurate Data ◽  
Young Leaves ◽  
Total Dna ◽  
Diversity Research

Mungbean (Vigna radiata L.) is one of important legume in Indonesia. G-SSR and EST-SSR markers had been widely used in mungbean genetic diversity research. DNA isolation and DNA amplification are required to obtain genetic information about mungbean to obtain accurate data on the genetic diversity of mungbean. This study aims to determine the Temperature of annealing (Ta) of the g-SSR and EST-SSR primer pairs. The total DNA was isolated from young leaves mungbean origin Pelalawan and the eight primer pairs of the g-SSR and EST-SSR were optimized. The optimal Ta for G2436, G3598, G2516, G7472, G0483. G1671, G3302, and G3427 were 50,55ºC, 51,15ºC,51,25ºC, 51,2ºC, 51,6ºC, 49,0ºC, 49,8ºC, and 52,8ºC respectively. Meanwhile, the optimal Ta for E51985, E19823, E24080, E22860, E26637, E16266, E11659, and E10675 were 52,2ºC, 54,4ºC, 52,5ºC,51,25ºC, 53,25ºC, 54ºC, 54,35ºC, and 53,6ºC respectively.

scholarly journals IDENTIFIKASI KERAGAMAN GENETIK KENTANG SUPEJOHN TRANSGENIK (Solanum tuberosum L. var supejohn)

EUGENIA ◽  
2012 ◽  
Vol 18 (3) ◽  
Author(s):  
Christine L.W. Lengkong ◽  
Jeany Polii-Mandang ◽  
Edy F. Lengkong
Keyword(s):  
Genetic Diversity ◽  
Dna Isolation ◽  
Rapd Analysis ◽  
Solanum Tuberosum L ◽  
Similarity Coefficient ◽  
Agarose Gel ◽  
Buffer Solution ◽  
Random Primers ◽  
Average Similarity ◽  
Polymorphic Bands

ABSTRACT   The aim of this study was to determine the genetic diversity of potato Supejohn transgenic that had been twenty-five times subcultured with exploration  method. The genetic variability was calculated using molecular marker Polimorphic Random Amplified DNA (RAPD) analysis of ten samples from four weeks planlets of Supejohn transgenic plants using ten random primers. DNA isolation of 10 samples using CTAB buffer  then measurement of the DNA qualification and quantification,  afterwards amplification of DNA by PCR using 10 random primers followed by electrophoresis on a 1% agarose gel with TAE 1x electrode buffer solution. Visualisation  the results used UV Transluminator to see DNA bands and the data of  polymorphic bands had been analized used the NTSYS-pc version of 1.07 program to obtain the similarity coefficient  and the dendrogram.The results showed that five random primers produced polymorphic DNA bands with 0.39 - 0.88 similarity coefficient  and the average similarity coefficient is 0.63 (63%) or the genetic diversity of the samples as many as 37%. Dendrogram formed eight distinct clusters corresponding similarity coefficient, the formation of clusters means that there is genetic diversity among the DNA samples. Keywords: potato Supejohn transgenic, RAPD, genetic diversity ABSTRAK Penelitian ini bertujuan untuk  menentukan keragaman genetik kentang Supejohn transgenik yang telah dua puluh lima kali disubkultur dengan menggunakan metode penelitian eksplorasi dengan penanda molekuler  Random Amplified Polimorphic DNA (RAPD) terhadap 10 sampel tanaman Supejohn transgenik, berumur empat minggu dan  menggunakan  10 primer random.  Isolasi DNA dari 10 sampel menggunakan buffer CTAB sesudah itu dilakukan pengukuran kualitas dan kuantitas  DNA, kemudian dilanjutkan dengan amplifikasi DNA secara PCR menggunakan 10 primer random diikuti dengan  elektroforesis pada gel agarose 1% dengan larutan penyanggah elektroda TAE 1x. Visualisasi hasil elektroforesis  menggunakan UV Transluminator untuk melihat pita DNA  dan analisis  data pita polimorfik  menggunakan program NTSYS-pc versi 1.07 sehingga didapatkan koefisien kesamaan dan dendrogram. Hasil penelitian menunjukkan bahwa lima primer random menghasilkan pita DNA polimorfik dengan koefisien kesamaan 0.39 – 0.88 dan rata-rata koefisien kesamaan yaitu 0.63 (63%) atau  keragaman  genetik sampel sebesar 37%.  Dendrogram  membentuk delapan cluster yang berbeda sesuai koefisien kesamaan,terbentuknya clusters mengartikan bahwa ada keragaman genetik antar sampel DNA. Eugenia Volume 18 No. 3  Desember 2012 Kata kunci : kentang Supejohn transgenik , RAPD, keragaman genetik

scholarly journals IDENTIFICATION AND CHARACTERISATION OF COI GENE IN FEMALE SUMATRAN ELEPHANT (Elephas maximus sumatranus) IN ELEPHANT TRAINING CENTRE, WAY KAMBAS NATIONAL PARK

2021 ◽  
Vol 7 (1) ◽  
pp. 1-4
Author(s):  
Elsa Virnarenata ◽  
Elly Lestari Rustiati ◽  
Priyambodo Priyambodo ◽  
Eko Agus Srihanto ◽  
Dian Neli Pratiwi
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Distance ◽  
National Park ◽  
Dna Isolation ◽  
Coi Gene ◽  
Training Centre ◽  
Genetic Characteristics ◽  
Individual Female ◽  
Way Kambas National Park

Sumatran elephant is a subspecies of endemic Asian elephants on the island of Sumatra and is included in the Red List of the International Union for Conservation of Nature (IUCN) with critically endangered status. The building of the Elephant Training Centre (ETC) in Way Kambas National Park (WKNP) is one of the conservation efforts of Sumatran elephants. Small and closed population size lead to an increased risk of inbreeding that triggers reduction in genetic variation and viability and increases the risk of extinction. The phylogenetic pattern of Sumatran elephants in Indonesia has shown a low population genetic diversity. Genetic diversity information is indispensable to support the direction of decision making in Sumatran elephant conservation policy. The DNA isolation of Sumatran elephants in ETC, WKNP has performed as a first step to trace its genetic variation. The advanced step of DNA isolation is the use of cytochrome oxidase subunit I (COI) gene for identification of genetic characteristics in Sumatran elephants. The COI gene is one of the genes on the mitochondrial genome and in molecular studies it is used as a genetic marker to study genetic characteristics between species and individuals. Identification and characterisation are done by sequencing process and data analysis in the form of electroforegram using Molecular Evolution Genetics Analysis (MEGA) software version 6.0. to see the genetic diversity of the female Sumatran elephant population in ETC, WKNP. Based on the results of the analysis it is indicated that the genetic distance of 24 individual female Sumatran elephant from PLG, TNWK is 0.000 with a homology value of 100%, strengthened by the construction of phylogenetic tree. The absence of genetic distance indicates a close genetic relationship, so it can be concluded all individual female Sumatran elephants in the PLG, TNWK is derived from one population group.

scholarly journals A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis

2006 ◽  
Vol 9 (1) ◽  
pp. 86-91 ◽  
Author(s):  
Maryam Sarwat ◽  
Madan Singh Negi ◽  
Malathi Lakshmikumaran ◽  
Akhilesh Kumar Tyagi ◽  
Sandip Das ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Terminalia Arjuna ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Genomic Dna Isolation ◽  
Standardized Protocol

scholarly journals AN OPTIMIZED DNA ISOLATION PROTOCOL ENABLES AN INSIGHT INTO MOLECULAR GENETIC BACKGROUND OF ENDEMIC Moltkia petraea (Tratt.) Griseb. FROM BOSNIA AND HERZEGOVINA

2018 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Jasna Hanjalić ◽  
Semir Dorić ◽  
Lejla Lasić ◽  
Belma Kalamujić Stroil ◽  
Selma Hasičić ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Dna Isolation ◽  
Molecular Genetic ◽  
Pcr Amplification ◽  
Rflp Analysis ◽  
Endemic Plant ◽  
Living Fossil ◽  
Dna Yield ◽  
Endemic Plant Species ◽  
Dna Isolation Protocol

The Dinaric endemic plant species Moltkia petraea (Tratt.) Griseb. is often called a "living fossil" of ancient Tertiary flora, with great importance for Bosnia and Herzegovina’s biodiversity. Considering its narrow and limited distribution range, insufficient data on the molecular background of this species is given so far. Due to the presence of various secondary metabolites that interfere with the DNA, isolation of nucleic acids from plant cells is known to be challenging. Even in closely related species it is necessary to optimize DNA isolation protocol in order to obtain high quality PCR amplifiable DNA. We collected 91 samples from five populations in Herzegovina. Doyle and Doyle (1987) CTAB protocol was modified by adding vitamin C (ascorbic acid) to the cell lysis buffer to improve DNA yield and quality. trnL(UAA) intron and nrDNA (ITS1, ITS2) molecular markers were applied to demonstrate amplifiability of isolated DNA and elucidate the intra- and interpopulation genetic diversity. Our results suggest a successful PCR amplification for 81% of the analyzed samples. PCR-RFLP analysis of trnL(UAA) revealed that all individuals in five populations have the same haplotype based on the obtained enzymatic profile for three enzymes (TaqI, HinfI, HindII). Alignment and comparison of ITS sequences didn’t reveal any hypervariable portion that could be informative in elucidating the genetic diversity of M. petraea populations. Further studies with additional application of microsatellite loci, RAPD and AFLP methods are necessary in an attempt to get insights into the genetic diversity of M. petraea.

scholarly journals Polimorfisme Penanda RAPD untuk Analisis Keragaman Genetik Pinusmerkusii di Hutan PendidikanUnhas

2017 ◽  
Vol 16 (2) ◽  
pp. 47
Author(s):  
Gusmiaty Gusmiaty ◽  
Muh. Restu ◽  
Asrianny Asrianny ◽  
Siti Halimah Larekeng
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Genetic Study ◽  
Dna Isolation ◽  
Dna Marker ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Tree Breeding ◽  
Genetic Characteristics ◽  
Dna Analyses

The aims of Genetic study of pinus identified stand in Unhas Experimental Forest is to analyses of genetic characteristics of stand, based on RAPD (Random Amplified Polymorphic DNA) marker. The study was conducted in Biotechnology and Tree Breeding Laboratory, Forestry Faculty, Hasanuddin University. The method are DNA isolation, Primers selection and RAPD analyses. DNA analyses of Pine with ten RAPD marker showed number of alel varietied and polymorphic. Coefficient of similarity in population have number of 0.15-0.73.Highest genetic distance is 0.9630 and lowest genetic distance is 0.2698. Number of genetic diversity of Pine in Experimental Forest Hasanuddin University is 0,489 and categorized highly.

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