STABILITAS PEMERIKSAAN HEMATOLOGI RUTIN PADA SAMPEL DARAH YANG DIDIAMKAN PADA SUHU RUANG MENGGUNAKAN CELL-DYN RUBY

Complete blood count (CBC) is one of the laboratory tests most often influenced by doctors. The use of a hematology analyzer offers a wider range of probe parameters. The pre-analytic stage accounts for 70% of errors, one of which is the delay of the examination. Changes in report results were reported due to changes in blood cell morphology due to EDTA additives and room temperature. The aim of this research is the disturbance of stability of the results of examination of various CBC parameters in blood samples that are left at room temperature for 24 hours using a hematology analyzer. This experimental laboratory research was conducted at the Pramita Jemur Andayani Clinical Laboratory. Blood samples were obtained from volunteers, stored at room temperature and subjected to immediate examination (control) and after a delay of 6, 12 and 24 hours (treatment). A total of 30 respondents, consisting of 8 men and 22 women. The mean age of the respondents was 22 ± 1 year. There was no difference in the results of the examination (p-value > 0.05) in the RBC, Hct MCV, MCHC, PLT and PDW. The results of the examination (p-value < 0.05) were found on Hgb, MCH, RDW, WBC, NEU, IG, MONO, EO, BASO, LYM, PLT and PDW. Delayed CBC examinations using the CELL-DYN Ruby hematology analyzer directly gave different results on several parameters ranging from 6 hours delay of examination.

ANALISA CEMARAN LOGAM BERAT (Pb, Cd, Zn) PADA MAKANAN DAN MINUMAN KEMASAN KALENG DENGAN MENGGUNAKAN METODE Spektrofotometri Serapan Atom (SSA)

Makanan dan minuman yang dikemas dalam kaleng memiliki kemungkinan untuk terkontaminasi logam berat yang berasal dari komponen penyusun kaleng. Kontaminasi logam berat bisa menyebabkan keracunan dan berbahaya bila masuk ke dalam system metabolism tubuh dengan jumlah yang melebihi batas yang telah ditetapkan dalam SNI dan BPOM. Penelitian ini bertujuan untuk menganalisa Timbal (Pb), Kadmium (Cd) dan seng (Zn) pada sarden, kornet, susu kaleng, sari buah, minuman berkarbonasi. pengambilan sampel dilakukan di dua lokasi berbeda yaitu pasar modern dan pasar tradisional, dengan tujuan agar dapat membandingkan perbedaan pada pasar modern dan pada pasar tradisional.Serapan logam diukur dengan menggunakan spektofotometri serapan atom (SSA) pada gelombang yang spesifik. Hasil penelitian adalah kadar cadmium (Cd) tidak teridentifikasi, kadar logam Timbal (Pb) pada sampel makanan dan minuman kaleng adalah 0.01 mg/kg, 0.018 mg/kg, 0.026 mg/kg, 0.012 mg/kg, 0.027 mg/kg, 0.027 mg/kg, 0.018 mg/kg, 0.015 mg/kg, 0.024 mg/kg dan 0.019 mg/kg, sementara itu cemaran Zn adalah 0.188 mg/kg, 0.169 mg/kg, 0.193 mg/kg, 0.184 mg/kg, 0.129 mg/kg, 0.105 mg/kg, 0.107 mg/kg, 0.098 mg/kg, 0.176 mg/kg, 0.152 mg/kg. Semua sampel makanan dan minuman kaleng tidak ada yang melebihi batas maksimum cemaran yang telah di tetap kan dalam SNI dan Badan Pengawas Obat dan Makanan. Kadar cemaran logam berat Pb dan Zn pada sampel makanan dan minuman kaleng dari pasar tradisional lebih tinggi daripada sampel makanan dan minuman kaleng dari pasar modern Kata kunci : Makanan-Minuman Kemasan Kaleng, SSA, Timbal, kadmium, seng

PENGARUH EKSTRAK BAWANG HITAM TERHADAP PENURUNAN KADAR GLUKOSA DARAH PADA MENCIT

Administration of oral hypoglycemic drugs and insulin is one of the most common pharmacological treatments given to people with diabetes mellitus. This treatment is carried out in the long term so that it causes side effects and is carried out continuously so that it will affect the financial condition of the diabetes mellitus patient. The purpose of this study was to determine the effect of black garlic extract on decreasing blood glucose levels in mice (mus musculus). This study uses the design of True Experimental design (pretest-posttest with control group design). The population is mice in Pusvetma Surabaya. Sampling method uses Non Probability Sampling Purposive Sampling type. Samples taken were 30 mice consisting of 15 intervention groups and 15 control groups. Data was collected using observation sheets, then analyzed using Independent sample t test and Dependent sample t test with a significance level of 5 0.05. The results showed: Using the test Independent sample T test obtained a significance value ρ = 0,000 It can be concluded that there is an effect of black onion extract to reduce blood glucose levels in mice. In the paired t-test test in the treatment group showed ρ = 0,000 that there were differences in blood glucose levels before being given extracts of garlic and after being given intervention. It is expected that black garlic can be used as an alternative medicine to reduce blood glucose levels.Keyword : Ekstrak of black garlic, Blood sugar levels, mus musculus

KORELASI ANTARA PROFIL HEMATOLOGI DENGAN PROCALCITONIN PADA PASIEN TERKONFIRMASI COVID-19

Covid-19 is an infectious disease caused by SARS-CoV 2 and has spread very quickly. Several laboratory examinations were carried out on Covid-19 patients including molecular examinations, hematological profiles, and serology. This study aims to determine the relationship between leucocyte, d-dimer, hemoglobin and thrombocyte against procalcitonin in patients with Covid-19. This was an observational analytic study with cross sectional design. A sample of 50 was taken selectively at Aisyiyah Siti Fatimah Tulangan Hospital in November 2020 - January 2021. Multiple linear regression test results showed relationship between leucocyte with procalcitonin (b = -0.64; p = 0.426), d-dimer with procalcitonin (b = 2.702; p = 0.010), hemoglobin with procalcitonin (b = 0.656; p = 0.255), and thrombocyte with procalcitonin (b = 0.037; p = 0.002) . Based on the results, it is found that there is relationship between leucocyte, d-dimer, hemoglobin, and thrombocyte againts procalcitonin in Covid-19 patients.

Uji Antioksidan Belimbing Wuluh dan Ampas Nanas Terhadap Kualitas Minyak Goreng Habis Pakai

Anti-Oxidant Tests of starfruit and pineapple pulp on the quality of Used Cooking Oils ABSTRACTUsed Cooking Oil is oil produced from frying residue and is carcinogenic because it causes oxidation, hydrolysis, and polymerization to produce toxic peroxides and free fatty acids that are difficult for the body to digest. Measurement of oil quality can be measured through 3 parameters, namely, the peroxide number, the acid number and the oil color. Efforts to control the quality of cooking oil are the addition of natural antioxidants, starch wuluh and pineapple pulp. Starfruit contains flavonoids, terpenoids, phenols and pectins as well as Vitamins C and A which can absorb free radicals in oil. Meanwhile, pineapple pulp contains high levels of vitamin C, flavonoids and polyphenols which can eat free radicals. This Research aims to determine the differences in the antioxidant power of starfruit and pineapple pulp to maintain the quality of used cooking oil. This research is experimental, which will study the antioxidant power of starfruit and pineapple pulp at the acid number, peroxidant and color intensity of used cooking oil. 3 grams of dry starfruit and 0.4 grams of dried pineapple pulp are added to each pad 100 mL of used cooking oil. The application of starfruit and pineapple pulp in used cooking oil had a significant effect on the 3 oil quality parameters, namely the acid number, the peroxide number and the oil color intensity, with a significance value of P <0.05. This is because the antioxidant content and composition of the two ingredients are different. Wuluh starfruit in the addition of 3 grams provides a fairly large antioxidant effect, while pineapple pulp at a concentration of 0.4 grams has shown anti-oxidant power so a higher concentration is needed to produce optimal antioxidant power. Keywords: Antioxidants, Starfruit, Pineapple Dregs

Vacutainer serum separator sebagai alternatif tabung penampung darah pada pemeriksaan kadar ureum

ABSTRACT Serum using is preferred for urea level because it does not use anticoagulants which can interfere with activity and reaction to the results. The tubes that are widely used to collect blood into serum are vacutainer serum separator and vacutainer plain.This researche aims to determine the degree of agreement s between vacutainer serum separator and vacutainer plain usage on serum urea level result.This research was cross sectional design and hold on October 2020 with subject were taken from thirty blood samples of health analyst students which taken randomly and had no history of disease or kidney function disorder. Each student was taken 6 ml of blood drawn using a venoject with each vacutainer containing 3 ml, so we had 60 data. The data were analyzed by descriptively and inferentially using the Interclass Correlation Coefficient (ICC) statistical test. From the descriptive analysis, the difference in mean levels was 0.35 mg/dL and the ICC statistical test resulted in a degree of agreement 0.745. The data were analyzed by descriptively and inferentially using the Interclass Correlation Coefficient (ICC) statistical test. From the descriptive analysis, the difference in mean levels was 0.35 mg/dL and the ICC statistical test resulted in a degree of agreement was 0.745. The calculation of the average working time between the vacutainer serum separator and the vacutainer plain was 4 minutes 38 seconds and 35 minutes 58 seconds. The analysis concluded that the vacutainer serum separator and the vacutainer plain could be used as an alternative of blood collecting tubes for urea level testing which proved to be no significant difference in the results from this research. Keywords : Urea level, Vacutainer Serum Separator, Vacutainer Plain

UJI KANDUNGAN HIDROQUINON PADA SEDIAAN KRIM RACIKAN DOKTER DAN KRIM PENCERAH WAJAH DENGAN MENGGUNAKAN SPEKTROFOTOMETER UV

Hydroquinon is a chemical used as a skin lightener. However, its effectiveness began to be questioned after the emergence of a study showing that hydroquinone can trigger cancer. This study aims to analyze the hydroquinone content of several doctor-made creams and branded facial lightening creams circulating in Denpasar. This research is an experimental research. The sample used consisted of five samples of doctor-made creams coded D1-D5, while branded lightening creams were coded P1-P10. Based on the results of the analysis, the wavelength of 293 nm is the wavelength that provides maximum absorption with the equation y = 0.023x + 0.005 with a value of R2 = 0.996. 0.023. The results of the analysis showed that all samples of the doctor's cream contained hydroquinone with an average of 11.9322 ppm or 1.1932% and did not exceed the BPOM regulations, namely 5% for cream preparations prescribed by doctors. And all samples of branded facial lightening creams in Denpasar City contain hydroquinone with an average hydroquinone level of 3,987 ppm or 0.3987% and do not exceed the BPOM regulations, which is 2% for whitening cream preparations that are sold freely without a doctor's prescription.

Evaluasi Analitik Hematology Analyzer Diatron Abacus 3 Pada Parameter Hematologi Rutin Di Laboratorium Hematologi Poltekkes Kemenkes Kalimantan Timur

Entering the era of globalization, manual tools in clinical laboratories have been replaced by full automatic devices. One of them is the Diatron Abacus Hematology analyzer 3. A relatively new hematology analyzer is required for analytical evaluation. Analytical evaluation is an evaluation of Diatron Abacus 3 on Sysmex KX 21 as a standard in RSUD I.A Moeis Samarinda, and it is very important to do this to assess the performance of the tool. Analytical evaluation is done by determining the value of accuracy, precision, and total error and linearity of measurement results from routine hematological examination parameters, which are erythrocytes, leukocytes, platelets, hemoglobin, and hematocrit. To determine the results of the hematology analyzer evaluation of Abacus 3 Diatron Analyzers on Routine Hematology Parameters in the Hematology Laboratory of the Health Ministry of Health, East Kalimantan. This type of research is observational descriptive, using a total sampling technique, and a sample of 40 complete K3EDTA blood specimens. Data processing using Microsoft Excel and SPSS 20 applications, analyzed using descriptive statistics. The observations were still included in the criteria for acceptance, accuracy or inaccuracy (d%) in erythrocytes 1.8%, leukocytes 8.0%, platelets -5.3%, hemoglobin 2.3% and hematocrit -1.7%; Precision or impression (CV%) in erythrocytes 4.2%, leukocytes 11.1%, platelets 6%, hemoglobin 3.9% and hematocrit 4.5%; Total errors in erythrocytes were 8.7%, leukocytes 17.9%, platelets 23.6%, hemoglobin 8.8% and hematocrit 9.1; Linearity of the measurement results against the routine hematological cell count values performed using Abacus 3 and Sysmex KX 21 has a positive relationship. Acceptance values are still included in the LOA on all parameters examined and still meet the criteria; the accuracy/bias value is smaller than the true value of the parameter being examined, except for leukocytes, which is greater; the precision on the five parameters is greater than the CV% Abacus 3 insert kit; The total error obtained by the TE value is greater than the TEA in the parameters examined, except for smaller platelets; Linearity of the measurement results against the calculated hematology cell routine values performed using Abacus 3 and Sysmex KX 21 have a positive relationship, meaning an increase in measurement values using Abacus 3 is followed by an increase in measurement values using Sysmex KX 21.

PROFIL TANIN PADA TEH SEDUH DENGAN PAPARAN SUHU PENYEDUHAN YANG BERBEDA

Tea is a beverage widely consumed by all levels of society as in addition to economic and easy to get, tea is also deemed to provide health benefits. One of the compounds that plays a role as antioxidant and antimicrobial in the tea for body health is tannin. The size of tannin content received by body depends on the way of processing tea before drinking. The higher the infusion temperature of tea infusion, it will make the content of tannin in the drinks is increasing. So the statement of problem in this research is whether there is any effect of infusion temperature on tannin content in tea infusion. This research aims to find out the effect of infusion temperature on tannin content in tea infusion. The type is experimental research. The sample is infused tea mostly consumed by people which is sold in market of Pacar Keling Surabaya. Total of samples used in this research is 40 grams of infused tea packages divided into two (2) treatments with 8 repetitions in each treatment. The variables are independent variable namely infusion temperature while the dependent variable is tannin content. From the examination of tannin content based on infusion temperature, it is known that the average of tannin content with infusion temperature of 700C and 1000C is 42.84% and 43.41% in a gram unit or 100 grams of infused water. The result of paired t test shows there is no effect of infusion temperature on tannin content in infusion tea with significant value more than 0.05 or 5%. Possible factor of no influence of temperature on tannin content is the length of infusion used which shall be not too long around 3-5 minutes. Password: Tea, Infusion Temperature, Tannin

Korelasi Profil Darah Dengan CRP Serum pada Pasien Diabetes Mellitus dengan Ulkus Diabetikum

Diabetes mellitus is a metabolic disorder that experiences hyperglycemia and causes various complications, including diabetic foot ulcers (DFU). Hyperglycemia can cause chronic inflammation and decreased function of immune cells. This study aims to determine the correlation between blood profiles and serum C-Reactive Protein (CRP) in diabetes mellitus patients with diabetic ulcers. A total of 30 blood samples of diabetes mellitus patients with blood tests and serum CRP. Blood profile examination used an automatic method with a hematology analyzer, erythrocyte sedimentation rate (ESR) using the Westergreen method, and serum CRP using a semi-quantitative method. In the correlation regression multiple test knowed that the parameters associated with CRP serum are hemoglobin (r = 23.553; p = 0.015), hematocrit (r = -8.678; p = 0.016), leukocyte count (r = 2.098; p = 0.004), and platelet count (r = -0.112; p = 0.002) because it has significance (p < 0.05). Meanwhile, the parameters of erythrocyte count (r = -8.717; p = 0.359), and ESR (r = -0.142; p = 0.109) had no effect on serum CRP levels because they had significance (p> 0.05). The conclusion of this study shows there is a strong correlation (r = 0.777) between blood profile and serum CRP in diabetes mellitus patients with diabetic foot ulcers.