scholarly journals Variation of eurycomanone content within and among populations of E. apiculata A.W. Benn.

2021 ◽  
Vol 905 (1) ◽  
pp. 012080
Author(s):  
Zulfahmi ◽  
Parjanto ◽  
E Purwanto ◽  
B Pujiasmanto ◽  
A T Sakya ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Environmental Factors ◽  
High Performance ◽  
Pearson Correlation ◽  
Genetic Material ◽  
Natural Populations ◽  
Base Population ◽  
Average Variation ◽  
Collection Sites

Abstract Information on the eurycomanone content of E. apiculata A.W. Benn from natural populations in Indonesia is unknown. This study aimed to assess the variation of eurycomanone content within and among populations of E. apiculata, determine the correlation of eurycomanone content with environmental factors, and determine the collection sites of genetic material for the establishment of the breeding base population of E. apiculata. The analysis of eurycomanone content was carried out using High-Performance Liquid Chromatography. This study found that the highest eurycomanone content was observed in the Rumbio population (9.86 mg/g) and the lower value was observed in the Pokomo population (4.44 mg/g). The average variation of eurycomanone content within the population was 25.72%. The coefficient of eurycomanone differentiation among populations was 84.33%, indicating that the variation of eurycomanone content among populations of E. apiculata was higher than the variation of eurycomanone content within-population (15.67%). Pearson correlation of eurycomanone content with population environmental factors showed no significant correlation. Based on the eurycomanone content and clustering, the Rumbio population can be selected as a source of the genetic material of E. apiculata for eurycomanone production via the breeding program in the future.

scholarly journals Variations of Secondary Metabolites among Natural Populations of Sub-Antarctic Ranunculus Species Suggest Functional Redundancy and Versatility

Plants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 234 ◽  
Author(s):  
Bastien Labarrere ◽  
Andreas Prinzing ◽  
Thomas Dorey ◽  
Emeline Chesneau ◽  
Françoise Hennion
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Environmental Changes ◽  
Environmental Gradients ◽  
Natural Populations ◽  
Phenotypic Traits ◽  
Geographical Regions ◽  
The Relationship

Plants produce a high diversity of metabolites which help them sustain environmental stresses and are involved in local adaptation. However, shaped by both the genome and the environment, the patterns of variation of the metabolome in nature are difficult to decipher. Few studies have explored the relative parts of geographical region versus environment or phenotype in metabolomic variability within species and none have discussed a possible effect of the region on the correlations between metabolites and environments or phenotypes. In three sub-Antarctic Ranunculus species, we examined the role of region in metabolite differences and in the relationship between individual compounds and environmental conditions or phenotypic traits. Populations of three Ranunculus species were sampled across similar environmental gradients in two distinct geographical regions in îles Kerguelen. Two metabolite classes were studied, amines (quantified by high-performance liquid chromatography and fluorescence spectrophotometry) and flavonols (quantified by ultra-high-performance liquid chromatography with triple quadrupole mass spectrometry). Depending on regions, the same environment or the same trait may be related to different metabolites, suggesting metabolite redundancy within species. In several cases, a given metabolite showed different or even opposite relations with the same environmental condition or the same trait across the two regions, suggesting metabolite versatility within species. Our results suggest that metabolites may be functionally redundant and versatile within species, both in their response to environments and in their relation with the phenotype. These findings open new perspectives for understanding evolutionary responses of plants to environmental changes.

scholarly journals Identification of Novel Environmental Substances Relevant to Pediatric Graves’ Disease

2021 ◽  
Vol 12 ◽  
Author(s):  
Qin Xia ◽  
Jingjing Liu ◽  
Xu Xu ◽  
Wei Gu ◽  
Kefeng Gu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Environmental Factors ◽  
High Performance ◽  
Protective Factor ◽  
Multiple Reaction Monitoring ◽  
Graves Disease ◽  
Total Iodine ◽  
Thyroid Autoantibody

Graves’ disease (GD) is the most common cause of hyperthyroidism, yet a relatively rare disease in the pediatric population. GD is a complex disorder influenced by both genetic and environmental factors. In this study, we aimed to find new environmental factors influencing the pathogenesis of GD. We investigated serum substances in 30 newly diagnosed GD children and 30 age- and gender-matched healthy controls. We measured total iodine by inductively coupled plasma-mass spectrometry (ICP-MS), analyzed perfluorinated compounds via ultra-high-performance liquid chromatography coupled with multiple reaction monitoring mass spectrometry (UHPLC-MRM-MS), and explored other environmental substances using ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF/MS) analysis. Twenty-nine single-nucleotide polymorphisms (SNPs) in eight genes related to GD were analyzed by SNaPshot. The serum total iodine was significantly higher in GD group, but its association with GD onset was weak, only with Exp(B) value near 1. The perfluorinated compound levels were not different between the two groups. More importantly, we found 16 environmental substances significantly different between GD and control groups, among which ponasterone A is a risk factor (p = 0.007 and Exp(B) = 14.14), while confertifoline is a protective factor against GD onset (p = 0.002 and Exp(B) = 0.001). We also identified 10 substances correlated significantly with thyroid indices in GD patients, among which seven associated with levels of the thyroid autoantibody TPOAb. No known SNPs were found predisposing GD. In this study, we explored a broad variety of environmental substances and identified novel factors that are potentially involved in the pediatric GD pathogenesis.

scholarly journals Quality Assessment of Different Species and Differently Prepared Slices of Zedoray Rhizome by High-Performance Liquid Chromatography and Colorimeter with the Aid of Chemometrics

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Fei Sun ◽  
Xiaolu Yang ◽  
Fei Liu ◽  
Ying Zhang ◽  
Shumei Wang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Pearson Correlation ◽  
Principal Component ◽  
New Approach ◽  
Colour Parameters ◽  
Pearson Correlation Analysis ◽  
Chemometric Tools

In this study, high-performance liquid chromatography (HPLC) and colorimeter were applied to evaluate the quality of different species and differently prepared slices of Zedoray Rhizome samples with the aid of chemometric tools. Fifty batches of Zedoray Rhizome samples from different species and forty-two batches of Zedoray Rhizome samples from differently prepared slices were collected. The quantitative method was developed using HPLC to simultaneously determine the contents of twelve chemical ingredients in Zedoray Rhizome. The colour parameters L, a, and b were measured by a colorimeter. Then, the collected data were analyzed by the principal component analysis and Pearson correlation analysis. The results showed that the proposed method was capable of accurately determining the contents of the twelve chemical ingredients and the colour parameters for the collected samples. There was a dramatic difference in the contents of the chemical ingredients and in the colour parameters among different species and differently prepared slices of Zedoray Rhizome samples. This study reveals that combining HPLC, colorimeter, and chemometric tools can provide a new approach to comprehensively evaluate the quality of Zedoray Rhizome samples.

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