scholarly journals Isolation and Characterization of Cellulolytic Bacteria Diversity in Peatland Ecosystem and Their Cellulolytic Activities

2021 ◽  
Vol 934 (1) ◽  
pp. 012028
Author(s):  
U M Batubara ◽  
M Mardalisa ◽  
S Suparjo ◽  
H U Maritsa ◽  
E Pujianto ◽  
...  
Keyword(s):  
Peat Soil ◽  
Methyl Cellulose ◽  
Cellulolytic Activity ◽  
Cellulolytic Bacteria ◽  
Determinative Bacteriology ◽  
Wetland Ecosystems ◽  
Isolation And Characterization ◽  
Identification Of Bacteria ◽  
Organic Deposits ◽  
Halo Zone

Abstract Peatlands are terrestrial wetland ecosystems formed from piles of organic matter that decompose into organic deposits. Peat soil has a high potential to produce cellulose which, can be reused by cellulolytic bacteria. This study aims to find out the potential strain of cellulolytic bacteria isolated from peatland ecosystems. The method used was experimental, sequentially, the stages are isolation and screening for cellulolytic bacteria, quantitative testing of cellulolytic activity, characterizing the morphology and physiology of bacteria, and the identification of bacteria based on Bergey’s Manual of Determinative Bacteriology. The screening results obtained seven isolates of cellulolytic bacteria capable of hydrolysed cellulose on 1% Carboxy Methyl Cellulose (CMC) Agar Medium, namely SPS1, SPS2, SPS 3, SDG1, SDG 2, SPW1, and SPW4. Three of seven isolates obtained the highest cellulolytic index sequentially, namely SPS2 of 2.82, SPS3 of 2.65, and SDG1 of 2.47. The cellulolytic activity was indicated by the value of a halo zone around the colonies on 1 % CMC medium after being dripped with Congo red. The halo zone is an early indication to determine the ability of bacteria to decompose cellulose. Based on Bergey’s Manual of Determinative Bacteriology showed that the three isolates had the same characteristics as the genus Bacillus, Lactobacillus and Corynebacterium.

scholarly journals Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw

2016 ◽  
Vol 8 (3) ◽  
pp. 314
Author(s):  
Ateng Supriyatna ◽  
Ukit Ukit
Keyword(s):  
Rice Straw ◽  
Organic Waste ◽  
Methyl Cellulose ◽  
Biology Education ◽  
Exponential Phase ◽  
Cellulolytic Activity ◽  
Cellulolytic Bacteria ◽  
Isolation Method ◽  
Hermetia Illucens ◽  
Waste Degradation

<p>A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrade cellulose. Screening and isolation method applied by using intestinal larvae obtained from larval gut vortex at a speed of 1500 rpm. Furthermore, dilution graded from 1 to 10 and grown in media CMC (carboxyl methyl cellulose) at 37 <sup>0</sup>C and incubated for 48 hours. Observations were made based on the characteristics of the microscopic, macroscopic, biochemical test, cellulolytic activity and the activity of cellulase enzymes selected bacteria. The results showed a 9 cellulolytic bacteria from the gut of the larvae. <em>Bacillus </em>sp. is a bacteria that have the highest potential with cellulolitic activity 2.1 mm (dz/dk), the exponential phase of hour at the 24<sup>th</sup>, and cellulase enzyme activity of 0.4 U/mL at pH 7 and 0.41 U/mL at pH 8.This research showed that the Black Soldier Flays Larvae (<em>Hermetia illucens</em>) have competencein organic waste degradation, because in Black Soldier Flays<em> Larvae’s</em> gut, cellulolitic enzyme is produced by cellulolitic bacteria, specially <em>Bacillus </em>sp,</p><p><strong>How to Cite</strong></p><p>Supriyatna, A., &amp; Ukit, U. (2016). Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (<em>Hermetia illucens</em>) Feeding with Rice Straw. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 314-320. </p>

scholarly journals Isolation of Cellulolytic Bacteria from Peat Soils as Decomposer of Oil Palm Empty Fruit Bunch

2017 ◽  
Vol 22 (1) ◽  
pp. 47-53
Author(s):  
. Gusmawartati ◽  
. Agustian ◽  
. Herviyanti ◽  
. Jamsari
Keyword(s):  
Oil Palm ◽  
Reducing Sugars ◽  
Peat Soil ◽  
Cellulolytic Activity ◽  
Empty Fruit Bunch ◽  
Cellulolytic Bacteria ◽  
Peat Soils ◽  
Bacterial Colony ◽  
Tropical Peat ◽  
Two Stages

The aim of the research was to find out potential strainsof cellulolytic bacteria isolated from two tropical peat soils and to studythe potency of the isolated bacteria to decompose oil palm empty fruit bunch (EFB). The research was carried out in two stages: (1) isolation of cellulolytic bacteria from peat soils and (2) testing the potency of isolated bacteria to decompose oil palm EFB. The cellulolytic bacteria were isolated from two peat soils, i.e. a natural peat soil (forest) and a cultivated peat soil (has been used as agriculture land). Isolation of cellulolytic bacteria was conducted by preparing a series dilution of culture solutions using a streak plate method in a carboxymethyl cellulose(CMC) selective medium.Isolates that were able to form clear zones surrounding their bacterial colony were further tested to study the potency of the isolates to decompose cellulose in oil palm EFB. The cellulolytic activity of the selected isolates were further determined via production of reducing sugars in an oil palm EFB liquid medium using Nelson-Somogyi method. The results showed that there are six isolates of cellulolytic bacteria that have been identified in two tropical peat soils used in the current study. Two isolates are identified in a natural peat soil (forest) and four isolates are identified in a cultivated peat soil. The isolates collected are identified as Bacillus sp., Pseudomonassp. and Staphylococcus sp. Among the isolates, an isolate of GS II-1 produces the highest concentration of reducing sugars, namely 0.1012 unitmL-1or 101 ppm, indicating that the isolate of GS II-1 is highly potential to decompose oil palm EFB. Therefore, the isolate of GS II-1 can be used as a decomposer in the bio-conversion processes of oil palm EFB.Keywords: isolation, bacteria, cellulolytic, oil palm empty fruit bunch (EFB), peat soil

scholarly journals Isolation and Characterization of Cellulolytic Bacteria from Historic Documents

2013 ◽  
Vol 26 (1) ◽  
pp. 22-31
Author(s):  
Shababa A. Bahjat ◽  
Adeeba younis Sherif ◽  
Musa’ab Hani Salih
Keyword(s):  
Cellulolytic Bacteria ◽  
Isolation And Characterization

Isolation of endo-1,4-b-D-glucanase producing Bacillus subtilis sp. from fermented foods and enhanced enzyme production bydeveloping the mutant strain

2017 ◽  
Author(s):  
Jakyeom Seo ◽  
Eunhye Park ◽  
Sung Sill Leek ◽  
Byeongwoo Kim ◽  
Teaksoon Shin ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Mutant Strain ◽  
Enzyme Production ◽  
Methyl Cellulose ◽  
Feed Additives ◽  
Fermented Foods ◽  
Wild Type ◽  
Cellulolytic Bacteria ◽  
16S Rrna Analysis ◽  
Significant Difference

Cellulolytic bacteria living in food can be applied to microbial feed additives to improve fiber digestion in animal feeds. In this study, a cellulase-producing bacteria was isolated from salted clam and treated with physical or chemical agents to enhance their enzyme production. The bacteria was identified as a strain of Bacillus subtilis on the basis of 16S rRNA analysis. Endo-1,4-b-D-glucanase (endoglucanase) was produced by the wild type using 0.4% carboxy-methyl-cellulose as a carbon source with maximal activity (0.04 U/mL) after 24 h incubation. Insoluble cellulose and oat spelt xylan were also used as carbon sources for investigation of exoglucanase and xylanase, however, these enzymes were not found in the culture supernatant. Maximum endoglucanase activity of Bacillus subtilis sp. was measured at 50°C and pH 5, respectively. Then, the strain was subjected to classical mutagenesis (UV-irradiation and chemical treatment) to improve endoglucanase production. A mutant strain, P11 treated with ethyl methyl sulfonate was finally selected. Mutant P11 was sub-cultured and tested for endoglucanase production, which was 0.05 U/mL after 24 h growth. The significant difference of endoglucanase production between wild type and mutant P11 was prolonged to 10th generation. Thus, the mutant strain was found to have enhanced endoglucanase production.

scholarly journals Isolation and characterization of lactic acid bacteria from fecal pellets, coelomic fluid, and gastrointestinal tract of Nypa worm (Namalycastis rhodochorde) from West Kalimantan, Indonesia

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
Ari Hepi Yanti ◽  
Tri Rima Setyawati ◽  
Rikhsan Kurniatuhadi
Keyword(s):  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Coelomic Fluid ◽  
Phenol Red ◽  
Cellulolytic Activity ◽  
Fecal Pellets ◽  
West Kalimantan ◽  
Isolation And Characterization

Abstract. Yanti AH, Setyawati TR, Kurniatuhadi R. 2020. Isolation and characterization of lactic acid bacteria from fecal pellets, coelomic fluid, and gastrointestinal tract of Nypa worm (Namalycastis rhodochorde) from West Kalimantan, Indonesia. Biodiversitas 21: 4726-4731. Lactic acid bacteria isolated from the intestinal tract as probiotic could be beneficial because they could trigger the growth of the host by enhancing digestibility, increasing their immune system, and inhibiting pathogenic bacteria through the enzymatic process or metabolites production. The purposes of this study were to identify and characterize lactic acid bacteria from fecal pellets, coelomic fluid, and gastrointestinal tract of Nypa worm (Namalycastis rhodochorde). Bacterial isolation was carried out by the pour plate method on de Mann Rogosa Sharp Agar (MRSA). Lactic Acid Bacteria (LAB) isolates were purified and then physiologically characterized by some biochemical tests. Cellulolytic activities were carried out by detecting a clear zone formation on CMC-congo Red Agar. There were 20 isolates of lactic acid bacteria from fecal pellets, coelom fluid, and gastrointestinal tract of nypa worm. Ten isolates (50%) had cellulolytic activity on CMC-phenol red agar. Eight isolates were closely related to the genus Lactobacillus, while two isolates were closely related to the genus Bacillus. These cellulolytic bacteria could be developed further as probiotic in Nypa worm feed.

scholarly journals Potential Detergent Compatibility And De-staining Ability Of Cellulolytic Bacteria

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Atia Iqbal ◽  
Mahnoor
Keyword(s):  
Cellulase Production ◽  
Maximum Activity ◽  
Cellulolytic Activity ◽  
Cellulolytic Bacteria ◽  
Gram Positive Bacteria ◽  
Detergent Compatibility ◽  
Biochemical Testing ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
Bacterial Cellulase

Cellulases are inducible enzymes that are synthesized by many microorganisms during their growth on cellulosic materials. Production of cellulase enzyme from bacteria has gained interest for applications in industries owing to their stability, catalytic activity and ease of production. In current study, total 40 cellulolytic bacteria were screened by agar well diffusion assay followed by Congo red stain. Cellulolytic bacteria were evaluated for detergent compatibility and de-staining ability. Bacterial cellulase production was optimized at different environmental conditions. Biochemical testing was done following Bergey’s manual. Ten cellulolytic bacteria selected, were gram positive. Bacteria showed best cellulolytic activity at 40oC to 60oC, at pH 9, lactose as carbon source and peptone as nitrogen source. Best hydrolysis zone shown was 45mm from strain MS22 and least zone was 6mm. The maximum detergent activity for surf excel was observed for G2 while the lowest was observed for MW22. Similarly, maximum activity for ariel and bonus were observed for RS5 and C3. G1 and C3 showed better clearance of ink as compared to other strains. In de-staining, G1, C1and MW17 showed better clearance of edible oil and G1, C2 and MW18 showed better clearance of grease. These promising cellulolytic bacteria can be used for various applications in different industries.

scholarly journals Isolation and Characterization of Cellulase Producing Bacteria for the Purpose of Converting Spent Mushroom Edible Canna-Waste into Organic Fertilizer  

2021 ◽  
Vol 31 (3) ◽  
pp. 12-19
Keyword(s):  
Organic Fertilizer ◽  
Quality Criteria ◽  
Bacterial Strains ◽  
16S Rrna Sequence ◽  
Production Chain ◽  
Cellulolytic Microorganisms ◽  
Isolation And Characterization ◽  
Indole 3 Acetic Acid ◽  
Halo Zone

Converting spent mushroom substrates into organic fertilizer helps to tackle the problem of pollution in edible canna starch processing villages and adds new value to the production chain of edible canna. To successfully turn the spent substrates into compost, there is certainly an indispensable role for cellulolytic microorganisms, in which Bacillus strains are always important. Several bacterial strains have been isolated from spent edible canna substrate after cultivation of monkey head mushroom in this study. Among isolated strains, the strain NDK5 has been selected exhibiting the highest cellulolytic activities with solubilization indexes of 6.14 and 18.3 mm for the ratio between the halo zone diameters and the colony diameters in the point cultivation method (SIratio) and the offset between the halo zone diameters and the agar hole diameters (SIoffset), respectively. The highest CMCase activity was 4.29 ± 0.071 U/ml. Morphological, physiological, biochemical, and 16S rRNA sequence analyses (100% homology with B. amyloliquefaciens sp. plantarum FZB42) were further carried out for the selected strain, leading to the identification of the strain as B. amyloliquefaciens sp. plantarum NDK5 strain. In addition, NDK5 was proved to have a capacity for synthesizing indole-3-acetic acid, a plant growth hormone, on an L-tryptophan-containing medium. Trial incubation of spent mushroom edible canna-substrate with the strain NDK5 showed increases in several quality criteria of the waste after 20 days of incubation, that meet the standard criteria for bio-organic fertilizer according to TCVN 7185:2002.

Cellulosomes localise on the surface of membrane vesicles from the cellulolytic bacterium Clostridium thermocellum

2019 ◽  
Vol 366 (12) ◽  
Author(s):  
Shunsuke Ichikawa ◽  
Satoru Ogawa ◽  
Ayami Nishida ◽  
Yuzuki Kobayashi ◽  
Toshihito Kurosawa ◽  
...  
Keyword(s):  
Clostridium Thermocellum ◽  
Cell Communication ◽  
Membrane Vesicles ◽  
Cellulolytic Enzymes ◽  
Crystalline Cellulose ◽  
Cellulolytic Bacterium ◽  
Cellulolytic Activity ◽  
Cellulolytic Bacteria ◽  
Degradation Activity ◽  
Enzyme Complexes

ABSTRACTMembrane vesicles released from bacteria contribute to cell–cell communication by carrying various cargos such as proteins, nucleic acids and signaling molecules. Cellulolytic bacteria have been isolated from many environments, yet the function of membrane vesicles for cellulolytic ability has been rarely described. Here, we show that a Gram-positive cellulolytic bacterium Clostridium thermocellum released membrane vesicles, each approximately 50–300 nm in diameter, into the broth. The observations with immunoelectron microscopy also revealed that cellulosomes, which are carbohydrate-active enzyme complexes that give C. thermocellum high cellulolytic activity, localized on the surface of the membrane vesicles. The membrane vesicles collected by ultracentrifugation maintained the cellulolytic activity. Supplementation with the biosurfactant surfactin or sonication treatment disrupted the membrane vesicles in the exoproteome of C. thermocellum and significantly decreased the degradation activity of the exoproteome for microcrystalline cellulose. However, these did not affect the degradation activity for soluble carboxymethyl cellulose. These results suggest a novel function of membrane vesicles: C. thermocellum releases cellulolytic enzymes on the surface of membrane vesicles to enhance the cellulolytic activity of C. thermocellum for crystalline cellulose.

Isolierung und Charakterisierung Antipyrin-abbauender Bakterien / Isolation and Characterization of Antipyrine-degrading Bacteria

1978 ◽  
Vol 33 (1-2) ◽  
pp. 120-123 ◽  
Author(s):  
Hartmut Blecher ◽  
Renate Blecher ◽  
Rudolf Müller ◽  
Franz Lingens
Keyword(s):  
Sole Source ◽  
Resting Cells ◽  
Determinative Bacteriology ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Different Strains

Abstract Five different strains of bacteria ultilizing antipyrine as sole source of carbon were isolated from soil. It was shown by morphological and physiological examinations, that the new isolates are closely related to strains selected with the herbicide chloridazon. A ll of these bacteria are charac­ terized by special features and cannot be classified according to Bergey’s Manual of Determinative Bacteriology.Part of the strains which were selected with antipyrine not only grow with antipyrine but also with chloridazon. The others cannot be grown on chloridazon. However, resting cells of the latter group convert chloridazon to its catechol derivative (5-amino-4-chloro-2 (2,3-dihydroxyphenyl) -3 (2H)-pyridazinone). In these bacteria a catechol-2,3-dioxygenase (catechol: oxygen 2,3-oxido-reductase, EC 1.13.11.2) was found which readily catalyzes the cleavage of the catechol derivative of antipyrine (2,3-dimethyl-l-(2,3-dihydroxyphenyl)-pyrazolone (5)). The enzyme shows only slight activity with the corresponding derivative of chloridazon.

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