scholarly journals Potential Detergent Compatibility And De-staining Ability Of Cellulolytic Bacteria

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Atia Iqbal ◽  
Mahnoor
Keyword(s):  
Cellulase Production ◽  
Maximum Activity ◽  
Cellulolytic Activity ◽  
Cellulolytic Bacteria ◽  
Gram Positive Bacteria ◽  
Detergent Compatibility ◽  
Biochemical Testing ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
Bacterial Cellulase

Cellulases are inducible enzymes that are synthesized by many microorganisms during their growth on cellulosic materials. Production of cellulase enzyme from bacteria has gained interest for applications in industries owing to their stability, catalytic activity and ease of production. In current study, total 40 cellulolytic bacteria were screened by agar well diffusion assay followed by Congo red stain. Cellulolytic bacteria were evaluated for detergent compatibility and de-staining ability. Bacterial cellulase production was optimized at different environmental conditions. Biochemical testing was done following Bergey’s manual. Ten cellulolytic bacteria selected, were gram positive. Bacteria showed best cellulolytic activity at 40oC to 60oC, at pH 9, lactose as carbon source and peptone as nitrogen source. Best hydrolysis zone shown was 45mm from strain MS22 and least zone was 6mm. The maximum detergent activity for surf excel was observed for G2 while the lowest was observed for MW22. Similarly, maximum activity for ariel and bonus were observed for RS5 and C3. G1 and C3 showed better clearance of ink as compared to other strains. In de-staining, G1, C1and MW17 showed better clearance of edible oil and G1, C2 and MW18 showed better clearance of grease. These promising cellulolytic bacteria can be used for various applications in different industries.

scholarly journals Antimicrobial Activity of Opuntia cochenillifera (L.) Mill Fruit and Cladode Extracts

2016 ◽  
Vol 3 ◽  
pp. 84-89
Author(s):  
Pooja Suryawanshi ◽  
G.M. Vidyasagar
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Maximum Activity ◽  
Methanolic Extracts ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

In the present study the antimicrobial activity of chloroform and methanolic extracts of Opuntiacochenillifera for both cladode and fruits was investigated. Methanolic extract was found to be an effective against the microbes namely, E. coli,Bacillus subtilis,Staphylococcus aureus, Pseudomonas aeruginosaCandidaalbicanC.glabrataC.haemuloniiC.Tropicalis. Maximum activity was observed against E. coli, B. Subtilisand,C.albican and C.glabrata at 40mg/ml. Agar well diffusion assay was used to determine minimum inhibitory concentration of all test microorganisms.

scholarly journals Antibacterial Potential of the Extracts of the Leaves of Azadirachta indica Linn.

2011 ◽  
Vol 3 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Saradhajyothi KOONA ◽  
Subbarao BUDIDA
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Micrococcus Luteus ◽  
Chloroform Extract ◽  
Gram Positive Bacteria ◽  
Good Antibacterial Activity ◽  
Agar Well Diffusion Assay ◽  
Negative Controls ◽  
Diffusion Assay ◽  
Antibacterial Potential

Azadirachta indica A. Juss (syn. Melia azadirachta) is well known in India and popularly known as Indian neem. To evaluate antibacterial potential, the agar well diffusion assay was used against Gram-negative and Gram-positive bacteria. Penicillin and Dimethyl sulfoxide were used as positive and negative controls, respectively. Methanol extract showed the highest and chloroform extract showed moderate to good antibacterial activity. Proteus vulgaris and Micrococcus luteus were the most susceptible bacteria while Bacillus subtilis was more resistant bacterium to the hexane, chloroform and methanol extracts of neem. The study recommended for the isolation and separation of bioactive compounds responsible for the antibacterial activity.

scholarly journals Enaminone-Derived Pyrazoles with Antimicrobial Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Mashooq Ahmad Bhat ◽  
Mohamed A. Al-Omar ◽  
Ahmed M. Naglah ◽  
Abdul Arif Khan
Keyword(s):  
Antimicrobial Activity ◽  
Gram Negative Bacteria ◽  
Significant Activity ◽  
Disc Diffusion ◽  
Initial Screening ◽  
Gram Positive ◽  
Gram Negative ◽  
Disc Diffusion Assay ◽  
Gram Positive Bacteria ◽  
Diffusion Assay

A series of pyrazoles derived from the substituted enaminones were synthesized and were evaluated for antimicrobial activity. All the compounds were characterized by the spectral data and elemental analysis. The synthesized compounds were initially screened for their antimicrobial activity against ATCC 6538, NCTC 10400, NCTC 10418, and ATCC 27853. During initial screening, compounds (P1, P6, and P11) presented significant antimicrobial activity through disc diffusion assay. These compounds were further evaluated for antimicrobial activity at different time points against Gram-positive and Gram-negative bacteria and presented significant activity for 6 hours. The activity was found to be greater against Gram-positive bacteria. In contrast at 24 hours, the activity was found only against Gram-positive bacteria except compound (P11), showing activity against both types of bacteria. Compound (P11) was found to have highest activity against both Gram-positive and Gram-negative bacteria.

Cellulolytic Microorganisms

2018 ◽  
pp. 34-47
Author(s):  
Amritha Govindrao Kulkarni ◽  
Ankala Bassappa Vedamurthy
Keyword(s):  
Animal Feed ◽  
Base Material ◽  
Renewable Resource ◽  
Fertilizer Application ◽  
Cellulase Production ◽  
Industrial Applications ◽  
Cellulolytic Bacteria ◽  
Microbial Conversion ◽  
Cellulolytic Microorganisms ◽  
Active Research

Cellulose is the most abundant polymer in plants and the microbial conversion of cellulose is a subject of active research. Currently, cellulase is commonly used in many industrial applications, especially in animal feed, textile, waste water, brewing and wine making. A challenging strategy for the efficient utilization of this renewable resource is to use it as a base material for the production of desired metabolites. This chapter therefore focuses on exploring the cellulase producing bacteria and optimizing the parameters for the enzyme cellulase under varied conditions. Cellulolytic bacteria can be exploited for cellulase production which serves wide applications in industries, pharmaceuticals and further, use of these CDB as bio-inoculants can be incorporated to enhance organic matter decomposition in soil to increase soil fertility and to minimize the fertilizer application. It finds wide applications in reducing the environmental pollution and promote sustainable agriculture.

scholarly journals Laundry Detergent Compatibility of Papain Like Protease Purified From Piper Betel Leaves

2018 ◽  
Vol 7 (3.3) ◽  
pp. 132
Author(s):  
A Mousami Shankar ◽  
Dr G.V.D. Sirisha ◽  
Dr K. Vijaya Rachel
Keyword(s):  
Laundry Detergent ◽  
Deae Cellulose ◽  
Maximum Activity ◽  
Native Page ◽  
Chromatographic Techniques ◽  
Detergent Compatibility ◽  
Ammonium Sulphate Precipitation ◽  
Sds Page ◽  
The Stability ◽  
Better Than

Enzymes have wide applications in detergent industry from early 1900’s. Mostly, clothes are soiled by protein based grime. Most of the detergents have either amylase / protease. Various sources were scrutinized for potent protease activity and Betel leaves were selected, the enzyme was then isolated, purified to homogeneity by ammonium sulphate precipitation, DEAE-Cellulose and gel permeation chromatographic techniques. The enzyme was monomeric in nature with a molecular mass of 38kDa as determined by native PAGE and SDS-PAGE. The enzyme shows maximum activity at 60oC and pH 4.0. The Km and Vmax of the enzyme were 4x10-3M and 54µmol/min/mg respectively. The enzyme was categorically inhibited by PCMB and iodo-acetamide suggesting it to have papain like nature. The stability of the enzyme is assessed over the stretch of alkaline pH and temperature. This evaluation validates the stability of the enzyme and its use in detergent formulations. It was evident that after adding the enzyme preparation the stains (tea, chocolate, blood) were removed much better than that of the controls, which affirms that papain like enzyme from betel leaves, enhances detergent activity.  

scholarly journals Antilisterial Activity of Bacteriocins Produced by Lactic Bacteria Isolated from Dairy Products

Foods ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1757
Author(s):  
Simona de Niederhäusern ◽  
Stefania Camellini ◽  
Carla Sabia ◽  
Ramona Iseppi ◽  
Moreno Bondi ◽  
...  
Keyword(s):  
Dairy Products ◽  
Raw Milk ◽  
Lactobacillus Bulgaricus ◽  
Molecular Characteristics ◽  
Soft Cheese ◽  
Wide Range ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
Lactic Bacteria ◽  
Selection Of

Sixty-nine Lactic Acid Bacteria (LAB) and bifidobacteria were isolated and identified from Italian dairy products (raw milk, cream, butter, soft cheese and yoghurt) to find new antimicrobial compounds to use as food bio-preservatives. All the isolates were preliminarily screened by the deferred antagonism method for bacteriocin production. Afterwards, to evaluate the release of bacteriocin in liquid medium, the Cell-Free Supernatant Fluid (CFSF) of the best producers was tested by agar well diffusion assay. The study allowed the selection of three bacteriocin producing strains (Enterococcus faecium E23, Bifidobacterium thermophilum B23 and Lactobacillus bulgaricus L21), endowed with the strongest and broadest inhibitory capability against the pathogen Listeria monocytogenes. The molecular characteristics and the chemical–physical properties of both producers and the respective bacteriocins were studied and compared. The results showed that E. faecium E23 was the best producer strain and its class IIa bacteriocins, called enterocin E23, exhibited a good spectrum of activity towards L. monocytogenes. Enterocin E23 was stable over a wide range of pH and at low temperatures for at least four months and, for this reason, it can be employed in refrigerated foods for the control of L. monocytogenes, the major concern in dairy products.

scholarly journals ANTIBACTERIAL ACTIVITY OF AQUEOUS ALCOHOLIC EXTRACT OF ABUTILON INDICUM AERIAL PARTS AGAINST E .FAECALIS – AN INVITRO STUDY

2017 ◽  
Vol 10 (5) ◽  
pp. 80
Author(s):  
Jannathul Feridoz ◽  
Anitha Roy
Keyword(s):  
Antibacterial Activity ◽  
Root Canal ◽  
Alcoholic Extract ◽  
Zone Of Inhibition ◽  
Aerial Parts ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
Microbroth Dilution ◽  
Abutilon Indicum

Objective: To evaluate the antibacterial activity of the aqueous alcoholic extracts of Abutilon indicum aerial parts against Enterococcus faecalis.Methods: Agar well diffusion assay, as well as microbroth dilution assays, were used for determination of antibacterial activity. The assay was done intriplicate, and chlorhexidine 0.2% was used as the control.Results: Different concentrations (200, 400, and 800 mg/mL) of the ethyl acetate extract of A. indicum aerial parts showed dose-dependantantibacterial activity. The maximum zone of inhibition was 30 mm at the maximum concentration used (800 mg/mL), and the minimum inhibitoryconcentration/minimum bactericidal concentration was found to be 200 mg/mL.Conclusion: This study suggests that the aqueous alcoholic extract of A. indicum aerial parts contains promising antibacterial substances which arehaving activity against E. faecalis. E. faecalis being one of the major threats for root canal failure during endodontic treatment; plants with antibacterialactivity against E. faecalis will be promising.Keywords: Abutilon indicum, Enterococcus faecalis, Antibacterial activity, Root canal failure.

scholarly journals INSECTICIDAL, ANTIBACTERIAL, AND ANTIRADICAL ACTIVITY OF NICOTIANA PLUMBAGINIFOLIA VIV. (SOLANACEAE)

2017 ◽  
Vol 10 (11) ◽  
pp. 159
Author(s):  
Prashith Kekuda Tr ◽  
Raghavendra Hl ◽  
Rajesh Mr ◽  
Avinash Hc
Keyword(s):  
Antiradical Activity ◽  
Radical Scavenging ◽  
Nicotiana Plumbaginifolia ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Ic50 Value ◽  
The Family ◽  
Arboviral Diseases ◽  
Agar Well Diffusion Assay ◽  
Dose Dependent

  Objectives: Nicotiana plumbaginifolia Viv. belongs to the family Solanaceae. The present study was carried out to determine insecticidal, antibacterial, and antiradical activity of aerial parts of N. plumbaginifolia.Methods: Extraction was carried out by maceration process using methanol. Insecticidal activity was assessed, in terms of larvicidal effect, against II and IV instar larvae of Aedes aegypti. Antibacterial activity was evaluated against a panel of 7 bacteria by agar well diffusion assay. Antiradical activity was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) free radical scavenging assays.Results: The extract was found to cause dose-dependent mortality of larvae of A. aegypti. The lethal concentration 50 value of extract for II and IV instar larvae was found to be 0.41 and 0.99 mg/ml, respectively. Extract was effective in inhibiting all bacteria. Gram-positive bacteria displayed marked susceptibility when compared to Gram-negative bacteria. Bacillus cereus and Escherichia coli were susceptible to highest and least extent, respectively. Extract scavenged both DPPH radicals and ABTS radicals dose dependently. Extract scavenged ABTS radicals more efficiently (inhibitory concentration [IC50] value 13.51 μg/ml) when compared to DPPH radicals (IC50 value 17.43 μg/ml).Conclusions: The plant N. plumbaginifolia appears to be a promising resource for developing agents with insecticidal, antibacterial, and antiradical activity. The observed bioactivities could be attributed to the presence of phytochemicals which are to be isolated, characterized, and subjected for bioactivity determinations. The plant can be used to prevent arboviral diseases, infectious diseases, and oxidative damage.

scholarly journals Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production byPaenibacillus terraeME27-1

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Yan-Ling Liang ◽  
Zheng Zhang ◽  
Min Wu ◽  
Yuan Wu ◽  
Jia-Xun Feng
Keyword(s):  
Cellulase Production ◽  
Biochemical Properties ◽  
Rrna Gene ◽  
Cellulolytic Bacteria ◽  
Bacterial Strains ◽  
Subtropical Region ◽  
Cmcase Activity ◽  
Degrading Bacteria ◽  
Screening And Identification ◽  
Natural Reserves

From different natural reserves in the subtropical region of China, a total of 245 aerobic bacterial strains were isolated on agar plates containing sugarcane bagasse pulp as the sole carbon source. Of the 245 strains, 22 showed hydrolyzing zones on agar plates containing carboxymethyl cellulose after Congo-red staining. Molecular identification showed that the 22 strains belonged to 10 different genera, with theBurkholderiagenus exhibiting the highest strain diversity and accounting for 36.36% of all the 22 strains. Three isolates among the 22 strains showed higher carboxymethyl cellulase (CMCase) activity, and isolate ME27-1 exhibited the highest CMCase activity in liquid culture. The strain ME27-1 was identified asPaenibacillus terraeon the basis of 16S rRNA gene sequence analysis as well as physiological and biochemical properties. The optimum pH and temperature for CMCase activity produced by the strain ME27-1 were 5.5 and 50°C, respectively, and the enzyme was stable at a wide pH range of 5.0–9.5. A 12-fold improvement in the CMCase activity (2.08 U/mL) of ME27-1 was obtained under optimal conditions for CMCase production. Thus, this study provided further information about the diversity of cellulose-degrading bacteria in the subtropical region of China and foundP. terraeME27-1 to be highly cellulolytic.

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