scholarly journals New molecular and phenotypic data on Mayamaea Arida (Bock) Lange-Bertalot (Bacillariophyta)

2021 ◽  
Vol 937 (2) ◽  
pp. 022002
Author(s):  
V B Bagmet ◽  
A Yu Nikulin ◽  
Sh R Abdullin ◽  
V Yu Nikulin ◽  
A A Gontcharov
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
Soil Sample ◽  
Rare Species ◽  
Shape Index ◽  
Plastid Gene ◽  
Phenotypic Data ◽  
Length Width ◽  
Scanning Electron

Abstract Mayamaea arida (Bock) Lange-Bertalot is a rare species, previously recorded from four locations in Europe only. The aim of this study is to report additional molecular, phenotypic and geographic data on M. arida. The strain of this species was isolated from the soil sample collected on 04.08.2018 in Iturup Island (Sakhalin Region, Russia) in Haplic Cambisol. The strain was identified using light and scanning electron microscopy. Phylogenetic analysis revealed that the M. arida was a member of the strongly supported Mayamaea generic clade and resolved as a basal lineage in the subclade comprised by M. atomus and M. terrestris. New data on partial rbcL plastid gene, chloroplasts morphology and nucleus, lower limit of the cell length, higher number of striae in 10 urn, shape index (length/width), teratological forms and distribution of species are presented.

To morphology, ecology and distribution of rare species Sellaphora hustedtii (Krasske) Lange-Bertalot et Werum (Bacillariophyta) from waterbodies of the Gydan Peninsula

2014 ◽  
Vol 48 ◽  
pp. 57-65 ◽  
Author(s):  
S. I. Genkal ◽  
M. I. Jarushina
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
River Basin ◽  
Rare Species ◽  
Western Siberia ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Diagnostic Features ◽  
Scanning Electron Microscopy Study ◽  
Scanning Electron

A rare species Sellaphora hustedtii, which is also new for the flora of Russia, has been found in waterbodies of the Messoyakha River basin (south-western part of the Gydan Peninsula, Western Siberia). The scanning electron microscopy study revealed variability of the main quantitative and qualitative diagnostic features of the species (length and width of the valve, number of striae and areolae in 10 μm, shape of the valve and structure of the raphe). New data on the ecology and distribution of the species allowed us to specify its diagnosis.

New perspectives on Nephridiacanthus major (Acanthocephala: Oligacanthorhynchidae) collected from hedgehogs in Iran

2020 ◽  
Vol 94 ◽  
Author(s):  
O.M. Amin ◽  
M. Sharifdini ◽  
R.A. Heckmann ◽  
M. Zarean
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
Chemical Analysis ◽  
Central Asia ◽  
Molecular Profile ◽  
X Ray ◽  
The Family ◽  
First Time ◽  
Scanning Electron

We describe morphological features not previously reported for this old acanthocephalan Nephridiacanthus major (Bremser, 1811 in Westrumb, 1821) Golvan, 1962 first described over 200 years ago. Our specimens were collected from long-eared hedgehog Hemiechinus auritus (Gmelin, 1770) (Erinaceidae) in Iran. We compare the morphometrics of our material with others previously reported from the same host in Iran, Russia, central Asia and Europe. Our specimens had markedly smaller proboscides, proboscis hooks and lemnisci than those reported from Russia and central Asia, but comparable measurements of other structures with specimens previously described from other collections. We document our new observations with scanning electron microscopy features not previously demonstrable by other observers and provide a chemical analysis of proboscis hooks using energy-dispersive X-ray analysis for the first time. The molecular profile of this acanthocephalan, based on 18S rDNA and cox1 genes, was generated for the first time. The phylogenetic analysis showed that N. major is placed in a clade of the family Oligacanthorhynchidae, well separated from the families Moniliformidae and Gigantorhynchidae.

scholarly journals Balantidium grimi n. sp. (Ciliophora, Litostomatea), a new species inhabiting the rectum of the frog Quasipaa spinosa from Lishui, China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 29 ◽  
Author(s):  
Weishan Zhao ◽  
Can Li ◽  
Dong Zhang ◽  
Runqiu Wang ◽  
Yingzhen Zheng ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
New Species ◽  
Body Shape ◽  
Ssu Rdna ◽  
The Body ◽  
Molecular Phylogenetic ◽  
A New Species ◽  
Scanning Electron

Balantidium grimi n. sp. is described from the rectum of the frog Quasipaa spinosa (Amphibia, Dicroglossidae) from Lishui, Zhejiang Province, China. The new species is described by both light microscopy (LM) and scanning electron microscopy (SEM), and a molecular phylogenetic analysis is also presented. This species has unique morphological features in that the body shape is somewhat flattened and the vestibulum is “V”-shaped, occupying nearly 3/8 to 4/7 of the body length. Only one contractile vacuole, situated at the posterior body, was observed. The phylogenetic analysis based on SSU-rDNA indicates that B. grimi groups together with B. duodeni and B. entozoon. In addition, the genus Balantidium is clearly polyphyletic.

scholarly journals Joermungandr bolti , an exceptionally preserved ‘microsaur’ from the Mazon Creek Lagerstätte reveals patterns of integumentary evolution in Recumbirostra

2021 ◽  
Vol 8 (7) ◽  
pp. 210319
Author(s):  
Arjan Mann ◽  
Ami S. Calthorpe ◽  
Hillary C. Maddin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
New Taxon ◽  
Phylogenetic Methods ◽  
Mazon Creek ◽  
Single Part ◽  
Scanning Electron

The Carboniferous Pennsylvanian-aged (309–307 Ma) Mazon Creek Lagerstätte produces some of the earliest fossils of major Palaeozoic tetrapod lineages. Recently, several new tetrapod specimens collected from Mazon Creek have come to light, including the earliest fossorially adapted recumbirostrans. Here, we describe a new long-bodied recumbirostran, Joermungandr bolti gen. et sp. nov., known from a single part and counterpart concretion bearing a virtually complete skeleton. Uniquely, Joermungandr preserves a full suite of dorsal, flank and ventral dermal scales, together with a series of thinned and reduced gastralia. Investigation of these scales using scanning electron microscopy reveals ultrastructural ridge and pit morphologies, revealing complexities comparable to the scale ultrastructure of extant snakes and fossorial reptiles, which have scales modified for body-based propulsion and shedding substrate. Our new taxon also represents an important early record of an elongate recumbirostran bauplan, wherein several features linked to fossoriality, including a characteristic recumbent snout, are present. We used parsimony phylogenetic methods to conduct phylogenetic analysis using the most recent recumbirostran-focused matrix. The analysis recovers Joermungandr within Recumbirostra with likely affinities to the sister clades Molgophidae and Brachystelechidae. Finally, we review integumentary patterns in Recumbirostra, noting reductions and losses of gastralia and osteoderms associated with body elongation and, thus, probably also associated with increased fossoriality.

Description of larva of Amblyomma romitii (Acari: Ixodidae) by optical and scanning electron microscopy, including porotaxy and phylogenetic analysis

2012 ◽  
Vol 60 (2) ◽  
pp. 271-280 ◽  
Author(s):  
Darci M. Barros-Battesti ◽  
Diego G. Ramirez ◽  
Janio dos Santos Sampaio ◽  
Katia M. Famadas ◽  
João Luiz H. Faccini ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
Scanning Electron

scholarly journals Comparative anatomy and phylogeny of the Forcipulatacea (Echinodermata: Asteroidea): insights from ossicle morphology

2019 ◽  
Vol 189 (3) ◽  
pp. 921-952 ◽  
Author(s):  
Marine Fau ◽  
Loïc Villier
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
Comparative Anatomy ◽  
Morphological Diversity ◽  
Consensus Tree ◽  
Sea Stars ◽  
Extant Species ◽  
Tree Resolution ◽  
Scanning Electron

Abstract A new phylogenetic analysis of the superorder Forcipulatacea is presented. Forcipulatacea is one of the three major groups of sea stars (Asteroidea: Echinodermata), composed of 400 extant species. The sampled taxa are thought to represent the morphological diversity of the group. Twenty-nine forcipulate taxa were sampled belonging to Asteriidae, Stichasteridae, Heliasteridae, Pedicellasteridae, Zoroasteridae and Brisingida. Specimens were dissected with bleach. Detailed description of the skeleton and the anatomy of the ossicles were investigated using scanning electron microscopy. Comparative anatomy allowed the scoring of 115 phylogenetically informative characters. The consensus tree resulting from the analysis recovers Asteriidae, Stichasteridae, Zoroasteridae and Brisingida as monophyletic. All types of morphological features contribute to tree resolution and may be appropriate for taxon diagnosis. The synapomorphies supporting different clades are described and discussed. Brisingida and Zoroasteridae are the best-supported clades. The potentially challenging position of Brisingida in the tree may be explained by homoplastic changes, but also by the presence of numerous non-applicable characters.

scholarly journals Identification by RAMAN Microscopy of magnesian vivianite formed from Fe2+, Mg, Mn2+and P043-’ in a Roman camp near fort Vechten, Utrecht, The Netherlands

2003 ◽  
Vol 82 (2) ◽  
pp. 209-214 ◽  
Author(s):  
J.T. Kloprogge ◽  
D. Visser ◽  
W.N. Martens ◽  
L.V. Duong ◽  
R.L. frost
Keyword(s):  
Crystal Structure ◽  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
The Netherlands ◽  
Soil Sample ◽  
Raman Microscopy ◽  
Low Intensity ◽  
Bending Modes ◽  
Scanning Electron

AbstractThe presence of a magnesian vivianite (Fe2+)2.5(Mg,Mn,Ca)0.5(PO4)2·8H2O, has been identified in a soil sample from a Roman camp near Fort Vechten, The Netherlands, using a combination of Raman microscopy and scanning electron microscopy. An unsubstituted vivianite and baricite were characterised for comparative reasons. The split phosphate-stretching mode is recognised around 1115, 1062 and 1015 cm−1, while the corresponding bending modes are found around 591, 519, 471 and 422 cm−1. The substitution of Mg and Mn for Fe2+in the crystal structure causes a shift towards higher wavenumbers compared to pure vivianite. As shown by the baričite sample substitution causes a broadening of the bands. The observed broadening however is larger than can be explained by substitution alone. The low intensity of the water bands, especially in the OH-stretching region between 2700 and 3700 cm−1indicates that the magnesian vivianite is partially dehydrated, which explains the much larger broadening than the observed broadening caused by substitution of Mg and Mn in vivianite and baričite.

scholarly journals Nutlet Micromorphology and Character Evolution of Some Species of Rochelieae (Boraginaceae) and Its Systematic Implications

2021 ◽  
Author(s):  
Mostafa Ebadi ◽  
Sedigheh Nikzat
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
Sequence Data ◽  
Character Evolution ◽  
Species Level ◽  
Phylogenetic Significance ◽  
Dna Sequence Data ◽  
Micro Morphology ◽  
Scanning Electron

Abstract Background: The macro/micro-morphology of nutlets in 11 species (and 22 accessions) of the Boraginaceae family was investigated by stereomicroscope and scanning electron microscopy to evaluate the taxonomic relevance of these traits. To evaluate the phylogenetic significance of the character evolution, available DNA sequence data from GenBank were combined with selected original nutlet data, and phylogenetic analysis was performed.Results: The Rochelieae nutlets' shape varied from ovoid (ovoid, ovoid-triangular, and ovoid-rectangular) to the pyramid. Six major patterns were recognized based on nutlet ultrastructure characters. Rocheliae is characterized by a transition from “without appendage” to “with tubercles and prickles” on the nutlet disk, and also by a shift from “lack of prickles” to “glossy prickles”.Conclusions: The results indicated that the nutlet ultrastructure pattern of Rochelieae is systematically informative at the genus level, but not at the species level. The results showed that glochid is not an ancestral trait but is a synapomorphy and the transition to this trait occurred in the genus Lappula. The Close boundary of nutlet microstructures between L. barbata and L. microcarpa was discussed.

Morphology and morphogenesis of Strongylidium pseudocrassum Wang and Nie, 1935, with redefinition of Strongylidium Sterki, 1878  (Protista: Ciliophora: Stichotrichia)

Zootaxa ◽  
2007 ◽  
Vol 1559 (1) ◽  
pp. 31-57 ◽  
Author(s):  
THIAGO DA SILVA PAIVA ◽  
INÁCIO DOMINGOS DA SILVA-NETO
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Division ◽  
Rare Species ◽  
De Novo ◽  
Current Knowledge ◽  
Northern Region ◽  
First Time ◽  
Scanning Electron

A population of the rare species Strongylidium pseudocrassum Wang and Nie, 1935, was discovered from a lagoon in the northern region of Rio de Janeiro, Brazil, and its morphology was investigated through protargol-impregnation, scanning electron microscopy and in vivo observations. Morphogenetic events of cell division and physiological reorganization were described for the first time to this species. It was found that S. pseudocrassum has the ventral cirral rows organized in the same manner as Pseudouroleptus caudatus Hemberger, 1985. However two independent primordia VI, one for the proter and other for the opisthe, are generated intrakinetally from the rightmost ventral cirral row. In addition, dorsal kineties replicate entirely “de novo” and do not fragment. Based on the data obtained, the genus Strongylidium was redefined and the species currently assigned to it were classified into five groups according to current knowledge on their ciliature and the combination S. lentum (Biernacka, 1963) nov. comb. is proposed.

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