scholarly journals Determination of prohibited ketone flavors in infant’s cosmetics by high performance liquid chromatography-tandem mass spectrometry

2021 ◽  
Vol 937 (2) ◽  
pp. 022023
Author(s):  
Fengyan Qiu ◽  
Fei Han ◽  
Yanping Hong
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Detection Limit ◽  
Tandem Mass Spectrometry ◽  
Recovery Rate ◽  
Rapid Screening ◽  
Tandem Mass ◽  
Good Precision ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Abstract A rapid screening method of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for five banned ketone fragrances in infant cosmetics has been established. The sample was ultrasonically extracted with acetonitrile, concentrated by nitrogen blowing. Methanol and 0.1% formic acid aqueous solution were used as mobile phases, and separated by gradient elution of HP C18 chromatographic column, and qualitative and quantitative by mass spectrometer. The results showed that the 5 banned ketone fragrances presented a good linear relationship within the mass concentration range of 0.5ug/kg∼50.0ug/kg, and the correlation coefficient r2>0.9983; the detection limit was 0.03ug/kg∼6.25ug/kg, and the limit of quantification is 0.1ug/kg∼ 0.5ug/kg; the average recovery rate of standard addition is between 84.3%∼ 100.6%, and the RSD is between 1.3%∼3.8%. This LC-MS/MS method is suitable for the rapid screening of 5 banned ketone fragrances in complex matrix infant cosmetics, and has the advantages of high recovery rate, low detection limit, and good precision. It can be used to further supplement and improve the technical support for hygiene regulations and quality supervision of banned ketones in cosmetics infant’s cosmetics.

scholarly journals Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

2011 ◽  
Vol 165 (6) ◽  
pp. 925-933 ◽  
Author(s):  
Femi Janse ◽  
Martinus J C Eijkemans ◽  
Angelique J Goverde ◽  
Eef G W M Lentjes ◽  
Annemieke Hoek ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Polycystic Ovary ◽  
Intraclass Correlation ◽  
Total Testosterone ◽  
Tandem Mass ◽  
Good Precision ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

ObjectiveThe measurement of serum testosterone in women is challenging due to lack of trueness, precision, and sensitivity of various available testosterone assays. Accurate assessment of testosterone in women is crucial especially in conditions associated with alleged over- or under-production of testosterone, such as in polycystic ovary syndrome (PCOS) or primary ovarian insufficiency (POI). The aim of this study was to measure and compare androgen concentrations in women with PCOS, POI, and female controls and to evaluate the performance of extraction RIA and liquid chromatography–tandem mass spectrometry (LC–MS/MS) in these women.DesignCross-sectional study.MethodsCarefully phenotyped women with POI (n=208) or PCOS (n=200) and 45 healthy, regularly cyclic female controls were included. Method comparison analyses were performed for total testosterone, androstenedione (AD), and DHEA, as measured by LC–MS/MS and extraction RIA.ResultsAll androgen levels were significantly elevated in women with PCOS compared with POI patients (P<0.05) and controls (P<0.05). Women with POI presented with similar androgen concentrations as controls, except for AD. Compared with measurements by extraction RIA, testosterone, DHEA, and AD concentrations measured by LC–MS/MS were systematically lower. However, using extraction RIA and LC–MS/MS, testosterone, DHEA, and AD measurements were shown to have good agreement as assessed by Bland–Altman analysis and intraclass correlation coefficient: 0.95 (95% confidence interval 0.94–0.91), 0.83 (0.79–0.86), and 0.96 (0.95–0.97) respectively.ConclusionsLC–MS/MS, compared with a labor-intensive extraction RIA, shows good precision, sensitivity, and high accuracy for measuring female testosterone, DHEA, and AD concentrations under various clinical conditions. LC–MS/MS, therefore, represents a convenient and reliable assay for both clinical and research purposes, where androgen measurement in women is required.

scholarly journals Disperse Solid-Phase Extraction Cleanup for the Determination of 1-Deoxynojirimycin in Mulberry Leaves with Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Lei Zhang ◽  
Yiran Zhou ◽  
Jing Meng ◽  
Jia Li
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Good Precision ◽  
Mulberry Leaves ◽  
Water Solvent ◽  
Chromatography Tandem Mass Spectrometry ◽  
Relative Standard ◽  
Liquid Chromatography Tandem Mass

A new determination method of 1-deoxynojirimycin (1-DNJ) in mulberry leaves based on ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been developed. Dried and crushed mulberry leaves’ sample was extracted by MeCN-water solvent, purified by graphitized carbon black (GCB) and primary secondary amine (PSA) to remove organic acids and pigments, and then analyzed after attenuation and filtration. The calibration curve showed linearity in the concentration range of 10–500 ng/mL, with the correlation coefficient of 0.998. Recoveries of spiked 1-DNJ at three fortification levels ranged from 94.6% to 96.4%, with relative standard derivation below 1.2%. Additionally, the matrix effect was assessed as negligible. Compared with methods by gas chromatography (GC) and liquid chromatography (LC) via real sample detection, the proposed method acquired better stability and detection efficiency. These results proved that this method has advantages of simple operation, complete purification, small pretreatment loss, good precision and accuracy, and high determination specificity, which is suitable for massive monitoring and precise quantitation of 1-DNJ in mulberry leaves.

scholarly journals Determination of Carrageenan in Livestock and Poultry Meat by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yanqin Sun ◽  
Xudong Zhu ◽  
Xixi Shen ◽  
Wei Wang
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Poultry Meat ◽  
Tandem Mass ◽  
Good Precision ◽  
Chromatography Tandem Mass Spectrometry ◽  
Relative Standard ◽  
Liquid Chromatography Tandem Mass ◽  
Ultrahigh Performance Liquid Chromatography

Ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) has become the main method for the detection and analysis of food additives because of its good separation, high selectivity, and high sensitivity. The aim of this study was to establish an UHPLC-MS/MS method that can quickly and accurately measure the content of carrageenan in livestock and poultry meat. Chromatographic separation was performed on an ACQUITY UPLC BEH HILIC C18 column (2.1 mm × 50 mm, 1.7 μm) using a gradient elution with methanol and 0.1% (v/v) formic acid in water as a mobile phase. The quantitative analysis was executed using a triple quadrupole mass spectrometer in which electrospray ionization, multiple reaction monitoring, and negative mode were operated. The retention time was about 1.3 min for carrageenan. The carrageenan content showed a good linear relationship from 0.05 to 1.00 g/kg. The limit of detection (LOD) was 0.06 g/kg, and the limit of quantification (LOQ) was 0.18 g/kg. The standards were spiked at three levels (low, medium, and high) and were analyzed in six replicates. The recovery values of carrageenan in pork, beef, lamb, chicken, and duck meat were 82.06–111.55%, 85.43–112.50%, 89.55–116.00%, 83.80–102.15%, and 82.41–110.90%, respectively. The relative standard deviations (RSDs) were all lower than 7.51%. The developed method shows a high recovery rate and good precision and can be used for the rapid detection of carrageenan in livestock and poultry meat.

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