Urease activity in an autotrophic bacteria Thiobacillus thiooxidans

THE PRESENCE OF AN ENDOGENOUS RESPIRATION IN THE AUTOTROPHIC BACTERIA

The Journal of General Physiology ◽

10.1085/jgp.25.4.617 ◽

1942 ◽

Vol 25 (4) ◽

pp. 617-622 ◽

Cited By ~ 7

Author(s):

K. G. Vogler

Keyword(s):

Oxygen Uptake ◽

Organic Materials ◽

Endogenous Respiration ◽

Thiobacillus Thiooxidans ◽

Autotrophic Bacteria ◽

Autotrophic Bacterium

It is shown that there exists in the autotrophic bacterium Thiobacillus thiooxidans a measurable oxygen uptake in the absence of the specific nutrient (sulfur). This respiration is shown to be due to the utilization of organic materials which must have been previously synthesized by the chemosynthetic process, providing evidence that autotrophic bacteria contain a dissimilatory process which involves the breakdown of organic materials and furnishes energy for the maintenance of the cell during periods in which the specific nutrient is absent. This is entirely in accord with the work of Bömeke (1939), who provided similar types of proof for Nitrosomonas and Nitrobacter. One may conclude, therefore, that autotrophic bacteria possess an endogenous respiration which involves the utilization of previously synthesized organic materials.

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STUDIES ON THE METABOLISM OF AUTOTROPHIC BACTERIA

The Journal of General Physiology ◽

10.1085/jgp.26.1.89 ◽

1942 ◽

Vol 26 (1) ◽

pp. 89-102 ◽

Cited By ~ 28

Author(s):

K. G. Vogler ◽

G. A. LePage ◽

W. W. Umbreit

Keyword(s):

Sulfuric Acid ◽

Organic Acids ◽

Bacterial Cell ◽

Sulfur Oxidation ◽

Energy Of Activation ◽

Detectable Effect ◽

Intact Cells ◽

Thiobacillus Thiooxidans ◽

Autotrophic Bacteria ◽

Autotrophic Bacterium

The data of this paper indicate that: 1. The "energy of activation" (µ) of sulfur oxidation by the autotrophic bacterium, Thiobacillus thiooxidans, is similar to that of other respirations. 2. The pH of the menstruum does not influence the respiration on sulfur between the limits of pH 2 to 4.8 once contact between the bacterial cell and the sulfur particle has been established but it does influence the rate at which such contact occurs. 3. The pO2 has little effect upon the respiration of this organism. 4. Most organic materials have no detectable effect upon the respiration of Thiobacillus thiooxidans, but the organic acids of terminal respiration seem to stimulate the respiration in the absence of oxidizable sulfur and certain of them inhibit sulfur oxidation. 5. In so far as inhibitor studies on intact cells are trustworthy, sulfur oxidation goes through iron-containing systems similar to cytochrome. It is possible that the oxygen contained in the sulfuric acid formed during sulfur oxidation is derived from the oxygen of the water.

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STUDIES ON THE METABOLISM OF AUTOTROPHIC BACTERIA

The Journal of General Physiology ◽

10.1085/jgp.26.1.103 ◽

1942 ◽

Vol 26 (1) ◽

pp. 103-117 ◽

Cited By ~ 19

Author(s):

K. G. Vogler

Keyword(s):

Co2 Fixation ◽

Physiological Condition ◽

Sulfur Oxidation ◽

Considerable Importance ◽

Resting Cells ◽

Oxidizing Agent ◽

Anaerobic Conditions ◽

Thiobacillus Thiooxidans ◽

Autotrophic Bacteria ◽

Growth Bound

In a study of chemosynthesis (the fixation of CO2 by autotrophic bacteria in the dark) in Thiobacillus thiooxidans, the data obtained support the following conclusions: 1. CO2 can be fixed by "resting cells" of Thiobacillus thiooxidans; the fixation is not "growth bound." 2. The physiological condition of the cell is of considerable importance in determining CO2 fixation. 3. CO2 fixation can occur in the absence of oxidizable sulfur in "young" cells. The extent of this fixation appears to be dependent upon the pCO2. 4. CO2 fixation can also occur under anaerobic conditions and the presence of sulfur does not influence such fixation. 5. However, in the CO2 fixation by cells in the absence of sulfur, only a limited amount of CO2 can be fixed. This amount is approximately 40 µl. CO2 per 100 micrograms bacterial nitrogen. After a culture has utilized this amount of CO2 it no longer has the ability to fix CO2 but releases it during its respiration. 6. Relatively short periods of sulfur oxidation can restore the ability of cells to fix CO2 under conditions where sulfur oxidation is prevented. 7. It is possible to oxidize sulfur in the absence of CO2 and to store the energy thus formed within the cell. It is then possible to use this energy at a later time for the fixation of CO2 in the entire absence of sulfur oxidation. 8. Cultures of Thiobacillus thiooxidans respiring on sulfur utilize CO2 in a reaction which proceeds to a zero concentration of CO2 in the atmosphere. 9. CO2 may act as an oxidizing agent for sulfur. 10. Hydrogen is not utilized by the organism. 11. It is possible to selectively inhibit sulfur oxidation and CO2 fixation.

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N-acetyl-cysteine and H. pylori urease activity

Gastroenterology ◽

10.1016/s0016-5085(01)82931-0 ◽

2001 ◽

Vol 120 (5) ◽

pp. A589-A589

Author(s):

H HUYNH ◽

R COUPER ◽

C TRAN ◽

A MUHAMED ◽

R BUTLER

Keyword(s):

Urease Activity ◽

Acetyl Cysteine ◽

H Pylori

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Diagnosis of helicobacter pylori infection: problems and prospects

Medical alphabet ◽

10.33667/2078-5631-2020-17-54-59 ◽

2020 ◽

pp. 54-59

Author(s):

A. S. Molostova ◽

N. S. Gladyshev ◽

A. V. Svarval ◽

R. S. Ferman ◽

A. B. Karasyova ◽

...

Keyword(s):

Helicobacter Pylori ◽

Urease Activity ◽

Diagnostic Methods ◽

Study Group ◽

Biochemical Method ◽

Antimicrobial Drugs ◽

Non Invasive ◽

Number Of Patients ◽

Pcr Method ◽

Positive Results

(HP) infection was performed using invasive and non-invasive methods. The study group consisted of 95 patients with dyspepsia. HP infection was detected in 47 patients (49.4 %). The expediency of using a set of diagnostic methods for detecting HP (PCR, immunochromatographic, bacteriological and method for determining urease activity) is proved. Most often (100 %) in patients HP infection was detected in biopsies using the PCR method. Somewhat less frequently it was detected when examining biopsies with an invasive biochemical method (AMA RUT Reader) (82 %) and fecal immunochromatographic method (83 %). Despite the fact that helicobacteriosis was detected bacteriologically in a small number of patients (24 %), this method is of particular value, since it allows you to assess the sensitivity to antimicrobial drugs and probiotics, and does not give false positive results.

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Nomenclature Abstract for Thiobacillus thiooxidans Waksman and Joffe 1922 (Approved Lists 1980).

The NamesforLife Abstracts ◽

10.1601/nm.1893 ◽

2003 ◽

Author(s):

Charles Thomas Parker ◽

Sarah Wigley ◽

George M Garrity

Keyword(s):

Thiobacillus Thiooxidans

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Determination of the prevalence of helicobacter pylori oral infection in smoking patients with chronic generalized periodontitis on the background of chronic hyperacidal gastritis during treatment

Problems of Uninterrupted Medical Training and Science ◽

10.31071/promedosvity2020.04.050 ◽

2020 ◽

Vol 40 (4) ◽

pp. 50-54

Author(s):

O. L. Zolotukhina ◽

◽

Ju. G. Romanova ◽

O. V. Maslov ◽

◽

...

Keyword(s):

Risk Factors ◽

Helicobacter Pylori ◽

Oral Cavity ◽

Urease Activity ◽

Rapid Test ◽

Oral Infection ◽

Helicobacter Pylori Infection ◽

Natural Origin ◽

Periodontal Tissues

Diseases of periodontal tissues occupy one of the leading positions among modern dental problems, namely the multifactorial nature of these diseases. In modern dental science, the issue of the development of periodontal pathology against the background of somatic pathology and risk factors remains relevant. Pathology of periodontal tissues in 68–90 % of cases is accompanied by chronic diseases of the gastrointestinal tract. Today, there is no doubt that Helicobacter pylori infection can be present in the biotopes of the oral cavity and can affect the course of periodontal pathology. As you know, smoking is one of the important risk factors for the development of inflammatory-dystrophic diseases of periodontal tissues, which can aggravate the course of the latter. The purpose of the work is to determine the prevalence of oral Helicobacter pylori infection in tobacco-dependent patients with chronic generalized periodontitis on the background of chronic hyperacid gastritis during treatment. Patients who received the proposed therapeutic and prophylactic complex (ultraphonophoresis procedures with the created gel «Apisan», and probiotic drug BioGaia ProDentis and angioprotective drug of natural origin — Detralex) showed a gradual decrease in the level of total urease activity and, as a consequence, a decrease the prevalence of Helicobacter pylori infection in the oral cavity according to the results of a urease rapid test with material from the oral cavity, both in the presence of a risk factor — smoking, and in its absence. The use of the proposed therapeutic and prophylactic complex proved to be effective in reducing the prevalence of oral Helicobacter pylori infection in smoking patients and patients who do not smoke, with chronic generalized periodontitis against the background of chronic hyperacidal gastritis associated with Helicobacter pylori.

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