Rapid Identification of Plasmid Replicon Type and Coexisting Plasmid-Borne Antimicrobial Resistance Genes by S1-Pulsed-Field Gel Electrophoresis-Droplet Digital Polymerase Chain Reaction

2021 ◽  
Author(s):  
Jialiang Xu ◽  
Ning Zhang ◽  
Ming Luo ◽  
Mengyu Wang ◽  
Ling Wang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Rapid Identification ◽  
Pulsed Field Gel Electrophoresis ◽  
Chain Reaction ◽  
Plasmid Replicon ◽  
Antimicrobial Resistance Genes ◽  
Polymerase Chain ◽  
Digital Polymerase Chain Reaction ◽  
Replicon Type

scholarly journals Epidemiological Investigation ofSalmonella Tileneby Pulsed-Field Gel Electrophoresis and Polymerase Chain Reaction

1997 ◽  
Vol 8 (6) ◽  
pp. 318-322 ◽  
Author(s):  
Chandar M Anand ◽  
Kevin Fonseca ◽  
Ken Longmore ◽  
Robert Rennie ◽  
Linda Chui ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Washington State ◽  
Pulsed Field Gel Electrophoresis ◽  
Dna Fingerprint ◽  
Rrna Genes ◽  
23S Rrna ◽  
Chain Reaction ◽  
Pulsed Field ◽  
Polymerase Chain

Pulsed-field gel electrophoresis (PFGE) and DNA fingerprinting by the polymerase chain reaction (PCR) were performed on 11 isolates ofSalmonella tilene. Five strains were from a cluster of human patients, six from sugar gliders and pygmy hedgehogs kept as family pets or from local pet retailers, and one isolate from the first North American case ofS tilenedescribed in Washington State in 1994. The PFGE restriction patterns showed all isolates to be similar. However, PCR using primers to the 16S and 23S rRNA genes ofEscherichia colidemonstrated that the Washington State isolate differed from the rest of the other isolates, which were all similar based upon their DNA fingerprint. This study indicates that reliance on one technique alone may be insufficient to show nuances between strains that are, in many respects, closely related.

Interinstitutional and Intrainstitutional Transmission of a Strain ofAcinetobacter baumanniiDetected by Molecular Analysis Comparison of Pulsed-Field Gel Electrophoresis and Repetitive Sequence–Based Polymerase Chain Reaction

2006 ◽  
Vol 27 (9) ◽  
pp. 981-983 ◽  
Author(s):  
S. Saeed ◽  
M. G. Fakih ◽  
K. Riederer ◽  
A. R. Shah ◽  
R. Khatib
Keyword(s):  
Polymerase Chain Reaction ◽  
Acute Care ◽  
Repetitive Sequence ◽  
Gel Electrophoresis ◽  
Care Facility ◽  
Pulsed Field Gel Electrophoresis ◽  
Chain Reaction ◽  
Pulsed Field ◽  
Polymerase Chain

Pulsed-field gel electrophoresis and repetitive sequence-based polymerase chain reaction provided comparable strain discrimination with minor discordance in typingAcinetobacter baumanniiclinical isolates from patients at our hospital and affiliated institutions. Typing revealed a cluster strain with intrainstitutional and interinstitutional spread during the study period. A long-term acute care facility may have been the reservoir.

scholarly journals Molecular Typing of Methicillin-sensitive and Methicillin-resistant Staphylococcus aureus: What Epidemiologists need to know from Plasmid Analysis to Whole Genome Sequencing.

2016 ◽  
Vol 6 (1) ◽  
pp. 31-43
Author(s):  
Ibrahim A. Al-Zahrani
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Pulsed Field Gel Electrophoresis ◽  
Whole Genome ◽  
Chain Reaction ◽  
Methicillin Resistant ◽  
Polymerase Chain

Methicillin-resistant Staphylococcus aureus is an important nosocomial pathogen. The early identification of an outbreak, making use of a rapid, precise and simple methicillin-resistant Staphylococcus aureus typing technique, can lead to prompt and effective precautions that avoid further spread of the infection. Pulsed-field gel electrophoresis is considered the gold standard for methicillin-resistant Staphylococcus aureus typing and has been recently supported by multilocus sequence typing. SmaI-multiplex polymerase chain reaction typing is a novel polymerase chain reaction -based technique that combines the principles of pulsed-field gel electrophoresis, with simplicity of polymerase chain reaction and can be used in many routine clinical laboratories. Whole genome sequencing typing is a second generation sequencing technology and may ultimately replace the traditional molecular typing methods. This review aims to survey existing molecular typing techniques for Staphylococcus aureus and to discuss information for each method in order to aid researchers and clinical professionals in the selection and implementation of an optimal technique.

scholarly journals Emerging ofptxP3 lineage inBordetella pertussis strains circulating in a population in northeastern Mexico

2018 ◽  
Vol 146 (16) ◽  
pp. 2096-2101 ◽  
Author(s):  
J. L. Gutiérrez-Ferman ◽  
L. Villarreal-Treviño ◽  
J. M. Ramírez-Aranda ◽  
A. Camacho-Ortiz ◽  
M. R. Ballesteros-Elizondo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Chain Reaction ◽  
Pertussis Infection ◽  
Allelic Combination ◽  
Global Spread ◽  
Northeastern Mexico ◽  
Polymerase Chain ◽  
Potential Impact

AbstractWe determined the molecular epidemiology ofBordetella pertussisisolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). SelectedB. pertussisisolates were further analysed; i.e. clonality was analysed by pulsed-field gel electrophoresis (PFGE) andptxP-ptxA, prn,fim2 andfim3 typing was performed by PCR and sequencing. Out of 11 864 analysed samples, 687 (5.8%) were positive for pertussis, with 244 (36%) confirmed by both culture and PCR whereas 115 (17%) were positive only by culture and 328 (48%) were positive only by PCR. One predominant clone (clone A,n= 62/113; 55%) and three major subtypes (A1, A2 and A3) were identified by PFGE. All 113 selected isolates had the allelic combinationptxP3-ptxA1.The predominant clone A and the three major subtypes (A1, A2 and A3) corresponded to the emerging genotypesptxP3-ptxA1-prn2-fim2-1-fim3-2 andptxP3-ptxA1-prn2-fim2-1-fim3-1. In conclusion, the presence of an endemic clone and three predominant subtypes belonging to the genotypesptxP3-ptxA1-prn2-fim2-1-fim3-2andptxP3-ptxA1-prn2-fim2-1-fim3-1were detected. This finding supports the global spread/expansion reported for these outbreaks associated genotypes.

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