In Vitro Primary Cell Culture as a Physiologically Relevant Method for Preclinical Testing of Human Oncolytic Adenovirus

Transfer of Bomirski amelanotic melanoma ceils from in vivo to in vitro growth conditions results in occurrence of rapid melanization in their cytoplasm. The melanized ceils from primary cell culture initiate tumours in hamsters, which do not contain traces of melanin and resemble typical amelanotic melanoma.

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Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?

Ciência Rural ◽

10.1590/0103-8478cr20200082 ◽

2020 ◽

Vol 50 (11) ◽

Author(s):

Haline Ballestero Fêo ◽

Luis Mauricio Montoya Flórez ◽

Ricardo Seiti Yamatogi ◽

Anderson do Prado Duzanski ◽

João Pessoa Araújo Junior ◽

...

Keyword(s):

Cell Culture ◽

Tumor Suppressor Genes ◽

Primary Cell Culture ◽

Gene Mutations ◽

Tp53 Gene ◽

Primary Cell ◽

Loss Of Function ◽

Gene Expressions

ABSTRACT: Canine transmissible venereal tumor (CTVT) is a transmissible neoplasm, which spreads naturally between dogs through the halogenic transfer of tumor cells, mainly during coitus. It is the oldest known tumoral lineage in nature and reports on gene mutations have been extended. Also, this tumor shares several genetic mutations with some cancers in humans, among them lung carcinomas, melanoma, prostate, breast, among other cancers. Thus, expression of tumor suppressor genes such as TP53, P21, and apoptosis-related genes such as BAX, BCL-2, and BCL-xL, both in vivo and in vitro (primary cell culture) were quantified. In the present study, the comparison of gene expression, the TP53 gene, in most cases, was shown to be high in the majority of tissues (65%) and primary cell culture (100%), while BCL-2, BCL-xL, and BAX presented variation among the animals analyzed. Moreover, in these situations, the results suggested that the apoptotic regulation of these genes did not occur for TP53. The P21 gene was shown to be mostly normal (70%); although, absence (6%) and underexpressions (24%) were also observed. Statistical analysis of the BCL-xL gene demonstrated significant differences between the tissues of the animals when compared to the cell cultures; however, to the other genes, no statistical difference was observed between the groups. Preliminarily, the results suggested the presence of alterations in the gene expressions of the TP53, P21, BAX, BCL-2 and BCL-xL leading to loss of function in these genes, which affect the tumorigenesis of CTVT.

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Effect of As2O3 on colorectal CSCs stained with ALDH1 in primary cell culture in�vitro

Oncology Letters ◽

10.3892/ol.2018.9110 ◽

2018 ◽

Author(s):

Ke Ning ◽

Wenlong Ning ◽

Xiaoting Ning ◽

Xueyan Wang ◽

Fei Zhou

Keyword(s):

Cell Culture ◽

Primary Cell Culture ◽

Primary Cell ◽

Culture In Vitro

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Rapid screening for individualized chemotherapy optimization of colorectal cancer: a novel conditional reprogramming technology-based functional diagnostic assay.

10.21203/rs.3.rs-35145/v1 ◽

2020 ◽

Author(s):

YingJie Li ◽

Dagang Guo ◽

Yihong Zhang ◽

Lin Wang ◽

Tingting Sun ◽

...

Keyword(s):

Colorectal Cancer ◽

Cell Culture ◽

Cell Biology ◽

Drug Sensitivity ◽

Primary Cell Culture ◽

Primary Cell ◽

Rapid Screening ◽

Primary Colorectal Tumor ◽

New Generation

Abstract In vitro patient tumor models such as patient-derived organoids (PDO) and conditionally reprogrammed (CR) cell culture are important for translational research and pre-clinical drug testing.In this study we present a personalized drug sensitivity test for late stage, potentially operable colorectal cancer (CRC) using patient-derived primary cell culture based on a new generation CR technology. We explored the clinical feasibility of using CR-based primary cell culture system to guide CRC chemotherapy, and established the correlation between in vitro drug sensitivity and patient clinical response.Our novel CR platform (termed i-CR) can be used to propagate primary colorectal tumor cells that represent individual patient tumors effectively by keeping the clonal heterogeneity and comparable drug responses.Therefore, our platform can be used to test and optimize therapeutic regimens pre-clinically, study cancer cell biology, and model tumor re-emergence to identify new targeted therapeutics from an effective personalized medicine standpoint.

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A Primary Cell Culture System for Human Cytotrophoblasts of Proximal Cytotrophoblast Cell Columns Enabling In Vitro Acquisition of the Extra-villous Phenotype

Placenta ◽

10.1016/j.placenta.2003.08.015 ◽

2004 ◽

Vol 25 (2-3) ◽

pp. 153-165 ◽

Cited By ~ 31

Author(s):

T. Nagamatsu ◽

T. Fujii ◽

T. Ishikawa ◽

T. Kanai ◽

H. Hyodo ◽

...

Keyword(s):

Cell Culture ◽

Culture System ◽

Primary Cell Culture ◽

Primary Cell ◽

Cell Culture System ◽

Cytotrophoblast Cell

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Development of an in vitro primary cell culture system from the penaeid shrimp, Penaeus stylirostris and Penaeus vannamei

Aquaculture ◽

10.1016/0044-8486(92)90024-f ◽

1992 ◽

Vol 101 (3-4) ◽

pp. 205-211 ◽

Cited By ~ 46

Author(s):

RenéA. Luedeman ◽

Donald V. Lightner

Keyword(s):

Cell Culture ◽

Culture System ◽

Primary Cell Culture ◽

Penaeid Shrimp ◽

Primary Cell ◽

Penaeus Vannamei ◽

Cell Culture System

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Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

BioMed Research International ◽

10.1155/2020/7120375 ◽

2020 ◽

Vol 2020 ◽

pp. 1-15 ◽

Cited By ~ 1

Author(s):

Katarzyna Wojtanowicz-Markiewicz ◽

Magdalena Kulus ◽

Sandra Knap ◽

Ievgenia Kocherova ◽

Maurycy Jankowski ◽

...

Keyword(s):

Cell Culture ◽

Epithelial Cells ◽

Primary Cell Culture ◽

Water Movement ◽

Inorganic Ions ◽

Primary Cell ◽

Lag Phase ◽

Luminal Epithelial Cells

Luminal epithelial cells are the first embryonic-maternal contact site undergoing very specific changes associated with reproductive processes. Cells prepare for embryo development by increasing their volume, with the help of aquaporins that provide a transcellular path of rapid water movement during the secretion and absorption of fluids, as well as connexins enabling the flow of inorganic ions and small molecules. In this work, we have examined how AQPs and Cx’s behave in luminal epithelium primary cell culture. Cells obtained from porcine specimen during slaughter were primarily in vitro cultured for 7 days. Their proliferation patterns were then analyzed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR. The results of these changes of gene of interest expression were analyzed on each of the seven days of the porcine luminal primary cell culture. Our study showed that the significant changes were noted in the case of Cx43, whose level of protein expression and distribution increases after 120 hours of culture, when the cells enter the lag phase, and maintains an upward trend until the end of the culture. We noted an increase in AQP4, AQP7, AQP8, and AQP11 levels throughout the entire culture period, while the largest differences in expression were found in AQP3, AQP4, and AQP10. The obtained results could become a point of reference for further in vivo and clinical research. Experiments conducted with these proteins showed that they influence the endometrial fluid content during the oestrous cycle and participate in the process of angiogenesis, which intensifies during endometrial development.

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