Correction to: A Novel Copolymer Poly(Lactide-co-β-Malic Acid) with Extended Carboxyl Arms Offering Better Cell Affinity and Hemacompatibility for Blood Vessel Engineering, by Wang W, et al. Tissue Engineering: Part A 2009; 15(1):65–73; DOI: 10.1089/ten.tea.2007.0394

2021 ◽  
Vol 27 (15-16) ◽  
pp. 1110-1111
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Malic Acid ◽  
Cell Affinity ◽  
Copolymer Poly ◽  
Blood Vessel Engineering

scholarly journals Chondroitin Sulfate/Polycaprolactone/Gelatin Electrospun Nanofibers with Antithrombogenicity and Enhanced Endothelial Cell Affinity as a Potential Scaffold for Blood Vessel Tissue Engineering

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Xiangqian Kong ◽  
Yuxiang He ◽  
Hua Zhou ◽  
Peixian Gao ◽  
Lei Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cell ◽  
Blood Vessel ◽  
Chondroitin Sulfate ◽  
Cell Attachment ◽  
Electrospun Nanofibers ◽  
Fiber Morphology ◽  
Cell Responses ◽  
Tissue Repair And Regeneration ◽  
Cell Affinity

AbstractElectrospun polymer nanofibers have gained much attention in blood vessel tissue engineering. However, conventional nanofiber materials with the deficiencies of slow endothelialization and thrombosis are not effective in promoting blood vessel tissue repair and regeneration. Herein, biomimetic gelatin (Gt)/polycaprolactone (PCL) composite nanofibers incorporating a different amount of chondroitin sulfate (CS) were developed via electrospinning technology to investigate their effects on antithrombogenicity and endothelial cell affinity. Varying CS concentrations in PG nanofibers affects fiber morphology and diameter. The CS/Gt/PCL nanofibers have suitable porosity (~ 80%) and PBS solution absorption (up to 650%). The introduction of CS in Gt/PCL nanofibers greatly enhances their anticoagulant properties, prolongs their coagulation time, and facilitates cell responses. Particularly, 10%CS/Gt/PCL nanofibers display favorable cell attachment, elongation, and proliferation. Thus, the Gt/PCL nanofibers containing a certain amount of CS could be excellent candidates as a promising tissue-engineering scaffold in blood vessel repair and regeneration.

scholarly journals Vascularization Strategies in Bone Tissue Engineering

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1749
Author(s):  
Filip Simunovic ◽  
Günter Finkenzeller
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Ex Vivo ◽  
Angiogenic Growth Factors ◽  
Hypoxic Conditions ◽  
Surgical Methods ◽  
Artificial Tissue

Bone is a highly vascularized tissue, and its development, maturation, remodeling, and regeneration are dependent on a tight regulation of blood vessel supply. This condition also has to be taken into consideration in the context of the development of artificial tissue substitutes. In classic tissue engineering, bone-forming cells such as primary osteoblasts or mesenchymal stem cells are introduced into suitable scaffolds and implanted in order to treat critical-size bone defects. However, such tissue substitutes are initially avascular. Because of the occurrence of hypoxic conditions, especially in larger tissue substitutes, this leads to the death of the implanted cells. Therefore, it is necessary to devise vascularization strategies aiming at fast and efficient vascularization of implanted artificial tissues. In this review article, we present and discuss the current vascularization strategies in bone tissue engineering. These are based on the use of angiogenic growth factors, the co-implantation of blood vessel forming cells, the ex vivo microfabrication of blood vessels by means of bioprinting, and surgical methods for creating surgically transferable composite tissues.

Spatiotemporal blood vessel specification at the osteogenesis and angiogenesis interface of biomimetic nanofiber-enabled bone tissue engineering

Biomaterials ◽  
2021 ◽  
pp. 121041
Author(s):  
Yuankun Zhai ◽  
Kevin Schilling ◽  
Tao Wang ◽  
Mirna El Khatib ◽  
Sergei Vinogradov ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Bone Tissue Engineering ◽  
Bone Tissue

scholarly journals Sustained VEGF delivery via PLGA nanoparticles promotes vascular growth

2010 ◽  
Vol 298 (6) ◽  
pp. H1959-H1965 ◽  
Author(s):  
Justin S. Golub ◽  
Young-tae Kim ◽  
Craig L. Duvall ◽  
Ravi V. Bellamkonda ◽  
Divya Gupta ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Femoral Artery ◽  
Release Kinetics ◽  
Aortic Ring ◽  
Plga Nanoparticles ◽  
Cardiovascular Medicine ◽  
Vessel Growth ◽  
Vessel Volume

Technologies to increase tissue vascularity are critically important to the fields of tissue engineering and cardiovascular medicine. Currently, limited technologies exist to encourage angiogenesis and arteriogenesis in a controlled manner. In the present study, we describe an injectable controlled release system consisting of VEGF encapsulated in poly(lactic- co-glycolic acid) (PLGA) nanoparticles (NPs). The majority of VEGF was released gradually over 2–4 days from the NPs as determined by an ELISA release kinetics experiment. An in vitro aortic ring bioassay was used to verify the bioactivity of VEGF-NPs compared with empty NPs and no treatment. A mouse femoral artery ischemia model was then used to measure revascularization in VEGF-NP-treated limbs compared with limbs treated with naked VEGF and saline. 129/Sv mice were anesthetized with isoflurane, and a region of the common femoral artery and vein was ligated and excised. Mice were then injected with VEGF-NPs, naked VEGF, or saline. After 4 days, three-dimensional microcomputed tomography angiography was used to quantify vessel growth and morphology. Mice that received VEGF-NP treatment showed a significant increase in total vessel volume and vessel connectivity compared with 5 μg VEGF, 2.5 μg VEGF, and saline treatment (all P < 0.001). When the yield of the fabrication process was taken into account, VEGF-NPs were over an order of magnitude more potent than naked VEGF in increasing blood vessel volume. Differences between the VEGF-NP group and all other groups were even greater when only small-sized vessels under 300 μm diameter were analyzed. In conclusion, sustained VEGF delivery via PLGA NPs shows promise for encouraging blood vessel growth in tissue engineering and cardiovascular medicine applications.

Blood Vessel Tissue Engineering

2013 ◽  
pp. 1237-1246
Author(s):  
Stacey C. Schutte ◽  
Robert M. Nerem
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel

scholarly journals Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering

Micromachines ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 679
Author(s):  
Uran Watanabe ◽  
Shinji Sugiura ◽  
Masayuki Kakehata ◽  
Fumiki Yanagawa ◽  
Toshiyuki Takagi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Microfluidic Device ◽  
Vascular Function ◽  
Laser Scanning ◽  
Crosslinking Reaction ◽  
Excitation Process ◽  
Hollow Structures ◽  
Photon Excitation

Engineered blood vessels generally recapitulate vascular function in vitro and can be utilized in drug discovery as a novel microphysiological system. Recently, various methods to fabricate vascular models in hydrogels have been reported to study the blood vessel functions in vitro; however, in general, it is difficult to fabricate hollow structures with a designed size and structure with a tens of micrometers scale for blood vessel tissue engineering. This study reports a method to fabricate the hollow structures in photodegradable hydrogels prepared in a microfluidic device. An infrared femtosecond pulsed laser, employed to induce photodegradation via multi-photon excitation, was scanned in the hydrogel in a program-controlled manner for fabricating the designed hollow structures. The photodegradable hydrogel was prepared by a crosslinking reaction between an azide-modified gelatin solution and a dibenzocyclooctyl-terminated photocleavable tetra-arm polyethylene glycol crosslinker solution. After assessing the composition of the photodegradable hydrogel in terms of swelling and cell adhesion, the hydrogel prepared in the microfluidic device was processed by laser scanning to fabricate linear and branched hollow structures present in it. We introduced a microsphere suspension into the fabricated structure in photodegradable hydrogels, and confirmed the fabrication of perfusable hollow structures of designed patterns via the multi-photon excitation process.

Tissue Engineering with Natural Tissue Matrices

2010 ◽  
Vol 76 ◽  
pp. 125-132 ◽  
Author(s):  
Akio Kishida ◽  
Seiichi Funamoto ◽  
Jun Negishi ◽  
Yoshihide Hashimoto ◽  
Kwangoo Nam ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Heart Valve ◽  
Biomechanical Properties ◽  
Biological Tissues ◽  
The Novel ◽  
Detergent Treatment ◽  
Decellularized Tissue ◽  
Natural Tissue ◽  
Porcine Tissues

Natural tissue, especially autologous tissue is one of ideal materials for tissue regeneration. Decellularized tissue could be assumed as a second choice because the structure and the mechanical properties are well maintained. Decellularized human tissues, for instance, heart valve, blood vessel, and corium, have already been developed and applied clinically. Nowadays, decellularized porcine tissues are also investigated. These decellularized tissues were prepared by detergent treatment. The detergent washing is easy but sometime it has problems. We have developed the novel decellularization method, which applied the high-hydrostatic pressure (HHP). As the tissue set in the pressurizing chamber is treated uniformly, the effect of the high-hydrostatic pressurization does not depend on the size of tissue. We have reported the HHP decellularization of heart valve, blood vessel, bone, and cornea. Furthermore, HHP treatments are reported to have the ability of the extinction of bacillus and the inactivation of virus. So, the HHP treatment is also expected as the sterilization method. We are investigating efficient processes of decellularization and recellularization of biological tissues to have bioscaffolds keeping intact structure and biomechanical properties. Our recent studies on tissue engineering using HHP decellularized tissue will be reported here.

Sign in / Sign up

Export Citation Format

Share Document