A Tunable, Three-Dimensional In Vitro Culture Model of Growth Plate Cartilage Using Alginate Hydrogel Scaffolds

The mechanisms underlying the spatiotemporal evolution of tumor ecosystems present a challenge in evaluating drug efficacy. In this Perspective, we address the use of three-dimensional in vitro culture models to delineate the dynamic interplay between the tumor and the host microenvironment in an effort to attain realistic platforms for assessing pharmaceutical efficacy in patients.

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Role of 1,25-dihydroxycholecalciferol in growth-plate cartilage: inhibition of terminal differentiation of chondrocytes in vitro and in vivo.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.87.17.6522 ◽

1990 ◽

Vol 87 (17) ◽

pp. 6522-6526 ◽

Cited By ~ 35

Author(s):

Y. Kato ◽

A. Shimazu ◽

M. Iwamoto ◽

K. Nakashima ◽

T. Koike ◽

...

Keyword(s):

Growth Plate ◽

Terminal Differentiation ◽

Growth Plate Cartilage

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An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols

International Journal of Molecular Sciences ◽

10.3390/ijms21155335 ◽

2020 ◽

Vol 21 (15) ◽

pp. 5335

Author(s):

Hana Barosova ◽

Bedia Begum Karakocak ◽

Dedy Septiadi ◽

Alke Petri-Fink ◽

Vicki Stone ◽

...

Keyword(s):

Prolonged Exposure ◽

Three Dimensional ◽

Adverse Outcomes ◽

Alveolar Epithelial Cells ◽

Proinflammatory Response ◽

Multiwalled Carbon ◽

Alveolar Epithelial ◽

Culture Model ◽

Air Liquid Interface

In vitro three-dimensional (3D) lung cell models have been thoroughly investigated in recent years and provide a reliable tool to assess the hazard associated with nanomaterials (NMs) released into the air. In this study, a 3D lung co-culture model was optimized to assess the hazard potential of multiwalled carbon nanotubes (MWCNTs), which is known to provoke inflammation and fibrosis, critical adverse outcomes linked to acute and prolonged NM exposure. The lung co-cultures were exposed to MWCNTs at the air-liquid interface (ALI) using the VITROCELL® Cloud system while considering realistic occupational exposure doses. The co-culture model was composed of three human cell lines: alveolar epithelial cells (A549), fibroblasts (MRC-5), and macrophages (differentiated THP-1). The model was exposed to two types of MWCNTs (Mitsui-7 and Nanocyl) at different concentrations (2–10 μg/cm2) to assess the proinflammatory as well as the profibrotic responses after acute (24 h, one exposure) and prolonged (96 h, repeated exposures) exposure cycles. The results showed that acute or prolonged exposure to different concentrations of the tested MWCNTs did not induce cytotoxicity or apparent profibrotic response; however, suggested the onset of proinflammatory response.

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EXPRESSION OF THE MICROFOLD CELLS IN THREE-DIMENSIONAL COCULTURE SYSTEM FOR IN VITRO CULTIVATION OF HUMAN NOROVIRUS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s5.t0052 ◽

2019 ◽

pp. 71-74

Author(s):

MIZANURFAKHRI GHAZALI ◽

SHARANIZA AB-RAHIM ◽

MUDIANA MUHAMAD

Keyword(s):

Three Dimensional ◽

In Vitro Cultivation ◽

Optimum Number ◽

M Cells ◽

Apical Surface ◽

Alginate Hydrogel ◽

Human Norovirus ◽

Protein Marker ◽

Hydrogel Beads

Introduction: Human Norovirus (HuNoV), a food-borne virus is the leading cause for acute gastroenteritis. However, its inability to propagate in vitropersists as major challenge in understanding HuNoV biology.Objective: This study aims to determine an effective culture system for HuNoV.Methods: The Caco-2 cells were cocultured with Raji B cells on alginate hydrogel beads. Scanning electron microscopy (SEM) was performed to confirmthe three-dimensional (3D) cells morphology. Western blot (WB) analysis was performed to detect protein markers expressed by Microfold (M) cells.Results: Optimization of Caco-2 cells monoculture in the alginate hydrogel beads showed optimum number of cells of 1 × 106 cells/ml, indicatedby the intact structure of the beads. Result of SEM showed clear structure of monoculture in the alginate hydrogel beads indicated by the presenceof smooth and regular apical surface while the coculture showed reduced apical surface of M cells. The result of WB showed downregulation ofUlex europaeus antibody expression.Conclusion: It is evident that the expression of M cells grown in 3D alginate hydrogel beads was successful, indicated by the structural morphologyseen under SEM as well as expression of protein marker by M cells. This established in vitro system is highly potential for cultivation of HuNoV.

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