Insulin-responsive amino peptidase follows the Glut4 pathway but is dispensable for the formation and translocation of insulin-responsive vesicles
2019 ◽
Vol 30
(12)
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pp. 1536-1543
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Keyword(s):
In fat and skeletal muscle cells, insulin-responsive amino peptidase (IRAP) along with glucose transporter 4 (Glut4) and sortilin, represents a major component protein of the insulin-responsive vesicles (IRVs). Here, we show that IRAP, similar to Glut4 and sortilin, is retrieved from endosomes to the trans-Golgi network by retromer. Unlike Glut4, retrograde transport of IRAP does not require sortilin, as retromer can directly bind to the cytoplasmic tail of IRAP. Ablation of IRAP in 3T3-L1 adipocytes shifts the endosomal pool of Glut4 to more acidic endosomes, but does not affect IRV targeting, stability, and insulin responsiveness of Glut4.
2019 ◽
Vol 91
(4)
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pp. 3021-3026
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Keyword(s):
2012 ◽
Vol 420
(3)
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pp. 576-581
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Keyword(s):
2013 ◽
Vol 61
(20)
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pp. 4850-4854
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Keyword(s):
2019 ◽
Vol 32
(4)
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pp. 210-218
Keyword(s):