Effect of soy protein and casein intake on intestinal absorption and lymphatic transport of cholesterol and oleic acid

A basal sterol-free diet consisting of casein, 18%; dextrin, 57%; oleic acid, 9%; U.S.P. salt mixture, 2%; yeast, 5%; and cellulose, 9%; along with 1% sulfasuxidine and 0.2% streptomycin sulfate was fed to eight rats during a control period of 12 days, the feces being collected under alcohol the last 4 days and assayed for digitonide sterol. The experiment was repeated with the addition of 28 mg of coprostanol to the diet. It was found that from 41 to 61% (average 48%) of the coprostanol was absorbed. The relation of chemical structure to the absorbability of cholesterol derivatives is discussed with the finding that all absorbable cholesterol derivatives have the ‘chair’ conformation, but are not alike in digitonide formation and Liebermann-Burchard color reaction.

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An Autoradiographic Study of the Intestinal Absorption of Palmitic and Oleic Acid

Acta Physiologica Scandinavica ◽

10.1111/j.1748-1716.1973.tb05525.x ◽

1973 ◽

Vol 89 (3) ◽

pp. 306-317 ◽

Cited By ~ 18

Author(s):

By Ulf Haglund ◽

Mats Jodal ◽

Ove Lundgren

Keyword(s):

Oleic Acid ◽

Intestinal Absorption ◽

Autoradiographic Study

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Triacylglycerol and cholesterol transport during absorption of glycerol trioleate vs. glycerol trielaidate

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1996.270.2.g268 ◽

1996 ◽

Vol 270 (2) ◽

pp. G268-G276

Author(s):

T. J. Kalogeris ◽

L. Gray ◽

Y. Y. Yeh ◽

P. Tso

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Intestinal Absorption ◽

Acid Composition ◽

Trans Fatty Acid ◽

Cholesterol Absorption ◽

Lymphatic Transport ◽

Cholesterol Transport ◽

Lipid Emulsions ◽

Intestinal Lymph

We used conscious, chronic lymph-fistula rats to compare intestinal lymphatic transport of glycerol trioleate (TO) vs. glycerol trielaidate (TE) and to determine the effect of TO vs. TE on absorption and transport of cholesterol. Rats were implanted with intestinal lymph fistulas and duodenal cannulas and then given intraduodenal infusions of lipid emulsions containing purified TO or TE (40 mumol/h) and cholesterol (7.8 mumol/h + 2 microCi [14C]cholesterol). Lymph samples were collected at 0, 2, 4, 5, 6, 7, and 8 h after the start of lipid infusion. Lymphatic output and luminal and gut wall recovery of radioactive lipid at 8 h were quantified. Triacylglycerol (TG) fatty acid isomers did not affect lymphatic output of TG; lymph TG fatty acid composition and output reflected infusate composition. Lymphatic output of cholesterol (mass and radioactivity) did not differ between groups; luminal and gut wall recovery of [14C]cholesterol was also similar between groups. Similar lymphatic transport of TG and cholesterol between triolein- and trielaidin-infused rats was maintained for up to 16 h after the cessation of an infused lipid load. These results indicate that TO and TE are transported into lymph similarly, and that cholesterol absorption and transport are similar irrespective of whether TO or TE is the TG source. The data suggest that trans fatty acid-induced hypercholesterolemia is not due to altered intestinal absorption and transport of cholesterol.

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