Automatic Simultaneous Determination of Total Serum Protein and Serum Albumin

1966 ◽  
Vol 45 (3) ◽  
pp. 277-282 ◽  
Author(s):  
John D. Rice
Keyword(s):  
Serum Albumin ◽  
Simultaneous Determination ◽  
Serum Protein ◽  
Total Serum Protein ◽  
Total Serum

Effect of protein concentration on the determination of digoxin in serum by fluorescence polarization immunoassay.

1984 ◽  
Vol 30 (11) ◽  
pp. 1826-1829 ◽  
Author(s):  
W H Porter ◽  
V M Haver ◽  
B A Bush
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Fluorescence Polarization ◽  
Protein Concentration ◽  
Total Serum Protein ◽  
Total Serum ◽  
Fluorescence Polarization Immunoassay ◽  
Total Protein Concentration ◽  
Protein Pellet

Abstract Determination of digoxin by fluorescence polarization immunoassay (FPIA) with the Abbott "TDx" is significantly influenced by the concentration of total serum protein. Each 10 g/L increase in serum protein results in an 8% decrease in measured digoxin. Studies with [3H]digoxin confirmed that digoxin binds to the protein pellet during the trichloroacetic acid precipitation step before the immunoassay. Serum protein, or equal concentrations of albumin or gamma-globulin, exert an equivalent effect on the apparent digoxin value. Because the total protein concentration of the assay calibrators is low (50 g/L) compared with its reference interval in serum (60-80 g/L), results by FPIA may be expected to be low by an average of 16% (range, 8-24%). Digoxin results by FPIA will be most nearly accurate when the calibrators include a total protein concentration of about 70 g/L. Patients' specimens with abnormally high or low protein content will give falsely high or low results for digoxin.

Increased serum albumin, γ globulin, immunoglobulin IgG, and IgG2 and IgG4 in autism

2002 ◽  
Vol 32 (8) ◽  
pp. 1457-1463 ◽  
Author(s):  
J. CROONENBERGHS ◽  
A. WAUTERS ◽  
K. DEVREESE ◽  
R. VERKERK ◽  
S. SCHARPE ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Serum Protein ◽  
Viral Infections ◽  
Autoimmune Disorder ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Total Serum Protein ◽  
Total Serum ◽  
Igg Subclass ◽  
Serum Concentrations

Background. Research on the biological pathophysiology of autism has found some evidence that immune alterations may play a role in the pathophysiology of that illness. As a consequence we expected to find that autism is accompanied by abnormalities in the pattern obtained in serum protein electrophoresis and in the serum immunoglobulin (Ig) and IgG subclass profile.Method. We examined whether subjects with autism showed changes in total serum protein (TSP) and the serum concentrations of albumin, α1 globulin, α2 globulin, β globulin and γ globulins, IgA, IgM and IgG and the IgG subclasses IgG1, IgG2, IgG3 and IgG4, compared with normal controls.Results. We found significantly increased concentrations of TSP in autistic subjects, which were attributable to increased serum concentrations of albumin and γ globulin. Serum IgG, IgG2 and IgG4 were also significantly raised. In autism there were significant and positive correlations between social problems and TSP and serum γ globulin and between withdrawal symptoms and TSP and serum albumin and IgG.Conclusions. The results suggest that autism is characterized by increased TSP, a unique pattern obtained in serum protein electrophoresis, i.e. increased serum albumin and IgG, and by a specific IgG subclass profile, i.e. increased serum IgG2 and IgG4. The increased serum concentrations of IgGs in autism may point towards an underlying autoimmune disorder and/or an enhanced susceptibility to infections resulting in chronic viral infections, whereas the IgG subclass skewing may reflect different cytokine-dependent influences on autoimmune B cells and their products.

scholarly journals PENGARUH PEMBERIAN SERBUK EKSTRAK KELOPAK BUNGA ROSELA (Hibiscus sabdariffa L.) TERHADAP SISTEM IMUN TIKUS SPRAGUE DAWLEY

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Mardiah Mardiah ◽  
Dwi Aryanti Nur’utami ◽  
Arti Hastuti
Keyword(s):  
Serum Albumin ◽  
Serum Protein ◽  
Total Serum Protein ◽  
Total Serum ◽  
Hibiscus Sabdariffa ◽  
Sprague Dawley

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian serbuk ekstrak kelopak bunga rosela terhadap sistem imun tikus Sprague Dawley. Parameter yang diamati adalah leukosit, differensial leukosit, organ limfoid (limpa dan hati), total serum protein dan serum albumin dari darah tikus Sprague Dawley. Hewan uji dibagi atas 4 kelompok yang masing-masing terdiri dari 5 ekor tikus. Kelompok pertama adalah kelompok normal, kelompok 2 diberikan stimuno forte dosis 1,35 mg/KgBB, kelompok 3 diberikan serbuk ekstrak rosela I dosis 40,5 mg/KgBB, dan kelompok 4 diberikan serbuk ekstrak rosela II dosis 81 mg/KgBB secara sonde selama 28 hari. Pada hari ke-0 dan hari ke-29 dilakukan pengambilan darah. Analisa data menggunakan perhitungan selisih, yang hasilnya dibandingkan dengan nilai normal tikus kondisi sehat. Berdasarkan hasil penelitian pemberin serbuk ekstrak rosela I dosis 40,5 mg/KgBB KgBB dapat mempertahankan fungsi sistem imun pada tikus Sprague Dawley dengan peningkatan nilai rata-rata jumlah neutrofil segmen sebesar 42,2%, total serum protein sebesar 10,99g/dl, bobot limpa relatif sebesar 0,22% dan bobot hati relatif sebesar 3,27% yang masih dalam batas normal hewan coba dalam kondisi sehat sehingga dapat memberikan efek protektif terhadap serangan antigen. Kata kunci :  Serbuk ekstrak,  rosela, sistem imun

Quantification of nonenzymically glycated albumin and total serum protein by affinity chromatography.

1984 ◽  
Vol 30 (3) ◽  
pp. 446-449 ◽  
Author(s):  
R W Yatscoff ◽  
G J Tevaarwerk ◽  
J C MacDonald
Keyword(s):  
Serum Protein ◽  
Protein Concentration ◽  
Glycated Albumin ◽  
Normal Subjects ◽  
Total Serum Protein ◽  
Total Serum ◽  
Exclusion Chromatography ◽  
Free Glucose ◽  
Chromatographic Procedure

Abstract We have evaluated an affinity-chromatographic procedure for determination of glycated albumin (GA) and glycated total serum protein (GSP). Recovery of these analytes was inversely related to free glucose concentration, thus necessitating removal of free glucose. For this we used molecular-exclusion chromatography on G-25 Sephadex, or dialysis, the latter procedure resulting in significantly (p less than 0.05) lower concentrations of GSP and GA. Total protein concentration and percent glycation are also inversely related, and so protein concentrations must be standardized before the assay. Within- and between-run CVs for both GSP and GA were less than 6.5 and 18%, respectively, the determination of GA being generally the more precise of the two. Labile glycated fractions, lipemia, icterus, hemolysis, and type of anticoagulant did not affect the results, but assay temperature did. Diabetic subjects showed substantially higher concentrations of GA and GSP than did normal subjects. Because of the life span of these analytes in circulation, their measurement may provide a short-term index of glycemic control.

A candidate reference method for determination of total protein in serum. I. Development and validation.

1981 ◽  
Vol 27 (10) ◽  
pp. 1642-1650 ◽  
Author(s):  
B T Doumas ◽  
D D Bayse ◽  
R J Carter ◽  
T Peters ◽  
R Schaffer
Keyword(s):  
Reference Material ◽  
Serum Protein ◽  
Reference Method ◽  
Total Serum Protein ◽  
Total Serum ◽  
National Bureau ◽  
Bovine Albumin ◽  
Color Development ◽  
Development And Validation

Abstract We developed a candidate Reference Method for measuring total serum protein by use of the biuret reaction. The method involves a previously described biuret reagent (Clin. Chem. 21: 1159, 1975) and Standard Reference Material (SRM) 927 bovine albumin (National Bureau of Standards) as the standard. At 25 degrees C, color development for 30 or 60 min provides identical serum protein values. Glucose (up to 10 g/L) and bilirubin (up to 300 mg/L) do not interfere. Hemoglobin, at 3 g/L, increases apparent serum protein by 0.4 g/L. The presence of dextran in serum causes easily detected turbidity, but this interference can be eliminated by centrifuging the reaction mixture. Therapeutic concentrations of ampicillin, carbenicillin, penicillin, oxacillin, nafcillin, chloramphenicol, cephalothin, and methicillin in blood do not interfere, nor do triglycerides up to 10 g/L. Within-run and day-to-day standard deviations of the method are 0.1 and 0.4 g/L, respectively.

Standards for Total Serum Protein Assays— A Collaborative Study

1975 ◽  
Vol 21 (8) ◽  
pp. 1159-1166 ◽  
Author(s):  
Basil T Doumas
Keyword(s):  
Human Serum Albumin ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Serum Protein ◽  
Bovine Serum ◽  
Total Serum Protein ◽  
Total Serum ◽  
Final Reaction ◽  
Protein Assays

Abstract We have studied the standardization of total serum protein assay with the biuret reaction. Standard solutions were prepared from lyophilized preparations of human serum albumin and bovine serum albumin, with corrections made for volatile material and ash contents. These solutions and a solution of crystalline albumin standard were analyzed with a new stable biuret reagent, to establish absorptivity values (values for the absorbance of a 1 g/liter final reaction mixture). The mean values obtained were 0.302, 0.292, and 0.290 for human serum albumin, bovine serum albumin, and the crystalline albumin, respectively. We believe that the established absorptivity value will improve the accuracy of serum protein determinations. We studied the linearity of the relation between color produced and protein concentration, with use of the solutions described above and a serum pool. The color adheres to Beer's law up to the highest concentration tested: 3 g/liter for HSA and BSA, and 2.8 g/liter for serum in the final reaction mixture. The new biuret reagent has been stable for one year at room temperature. We recommend the use of bovine serum albumin as a primary standard for serum protein assays. It is inexpensive, easily available, and exhibits the best linearity in the biuret reaction.

scholarly journals Protein deficiency during pregnancy and lactation impairs glucose-induced insulin secretion but increases the sensitivity to insulin in weaned rats

1998 ◽  
Vol 80 (3) ◽  
pp. 291-297 ◽  
Author(s):  
Márcia Q. Latorraca ◽  
Everardo M. Carneiro ◽  
Antonio C. Boschero ◽  
Maria A. R. Mello
Keyword(s):  
Insulin Secretion ◽  
Serum Albumin ◽  
Serum Protein ◽  
Serum Insulin ◽  
Serum Glucose ◽  
Total Serum Protein ◽  
Total Serum ◽  
Fetal Life ◽  
Suckling Period ◽  
Protein Serum

We studied glucose homeostasis in rat pups from dams fed on a normal-protein (170 g/kg) (NP) diet or a diet containing 60 g protein/kg (LP) during fetal life and the suckling period. At birth, total serum protein, serum albumin and serum insulin levels were similar in both groups. However, body weight and serum glucose levels in LP rats were lower than those in NP rats. At the end of the suckling period (28 d of age), total serum protein, serum albumin and serum insulin were significantly lower and the liver glycogen and serum free fatty acid levels were significantly higher in LP rats compared with NP rats. Although the fasting serum glucose level was similar in both groups, the area under the blood glucose concentration curve after a glucose load was higher for NP rats (859 (sem 58) mmol/l per 120 min for NP rats v. 607 (sem 52) mmol/l per 120 min for LP rats; P < 0.005). The mean post-glucose increase in insulin was higher for NP rats (30 (sem 4.7) nmol/l per 120 min for NP rats v. 17 (sem 3.9) nmol/l per 120 min for LP rats; P < 0.05). The glucose disappearance rate for NP rats (0.7 (sem 0.1) %/min) was lower than that for LP rats (1.6 (sem 0.2)%/min; P < 0.001). Insulin secretion from isolated islets (1 h incubation) in response to 16.7mmol glucose/l was augmented 14-fold in NP rats but only 2.6-fold in LP rats compared with the respective basal secretion (2.8 mmol/l; P < 0.001). These results indicate that in vivo as well as in vitro insulin secretion in pups from dams maintained on a LP diet is reduced. This defect may be counteracted by an increase in the sensitivity of target tissues to insulin.

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