Detection of Interferon-Tau in Uterine Vein Blood Using a Highly Sensitive and Specific Radioimmunoassay.

2012 ◽  
Vol 87 (Suppl_1) ◽  
pp. 183-183
Author(s):  
Alfredo Q. Antoniazzi ◽  
Andrea M. Latimer ◽  
Jared J. Romero ◽  
Gordon D. Niswender ◽  
Fuller W. Bazer ◽  
...  
Keyword(s):  
Interferon Tau ◽  
Specific Radioimmunoassay ◽  
Highly Sensitive

Urinary excretion of glycated protein determined with a specific radioimmunoassay

1991 ◽  
Vol 37 (4) ◽  
pp. 504-507 ◽  
Author(s):  
Chizuko Ukita ◽  
Mitsushige Nishikawa ◽  
Akira Shouzu ◽  
Mitsuo Inada
Keyword(s):  
Urinary Excretion ◽  
Normal Subjects ◽  
Mean Values ◽  
Specific Radioimmunoassay ◽  
Glycated Protein ◽  
Highly Sensitive ◽  
Significant Difference ◽  
Group A ◽  
The Mean ◽  
Group B

Abstract We developed a simple and highly sensitive RIA for glycated protein (GP), and used it to measure GP in serum and urine from 15 normal controls and 30 diabetics (14 with urinary excretion rate of albumin, Ualb less than 15 micrograms/min, group A; nine with 15 less than or equal to Ualb less than or equal to 150 micrograms/min, group B; and seven with Ualb greater than 150 micrograms/min, group C). The mean serum concentration of GP was above normal in all groups of diabetics, and the mean glycation ratios of serum protein (SGP) were higher in groups B and C than in normal subjects. Urinary concentrations of GP also were increased in groups B and C, although the glycation ratio of urinary protein (UGP) was decreased in group C. Consequently, the selectivity of urinary excretion of GP (UGP/SGP) was significantly decreased in group C. Moreover, there was a significant difference in the mean values of selectivity between groups of patients with various degrees of retinopathy. We suggest that measurements of serum and urinary GP are useful to evaluate the progression of diabetic complications.

RADIOIMMUNOASSAY OF 3,3′-DI-IODOTHYRONINE IN UNEXTRACTED SERUM: THE EFFECT OF ENDOGENOUS TRI-IODOTHYRONINE

1978 ◽  
Vol 79 (3) ◽  
pp. 357-362 ◽  
Author(s):  
T. J. VISSER ◽  
L. M. KRIEGER-QUIST ◽  
R. DOCTER ◽  
G. HENNEMANN
Keyword(s):  
Human Serum ◽  
Limit Of Detection ◽  
Cross Reactivity ◽  
Transport Proteins ◽  
Normal Serum ◽  
Serum Concentrations ◽  
Specific Radioimmunoassay ◽  
Highly Sensitive ◽  
The Mean ◽  
Mean Concentration

The development of a highly sensitive and specific radioimmunoassay for 3,3′-di-iodothyronine (3,3′-T2) is described. The assay was applied to the measurement of 3,3′-T2 in unextracted human serum and used 8-anilino-l-naphthalene-sulphonic acid to inhibit the binding of 3,3′-T2 to serum transport proteins. The lower limit of detection of the assay was 2 fmol 3,3′-T2 per tube, which corresponded to 10 pmol 3,3′-T2/l serum. The mean concentration of 3,3′-T2 in normal serum was found to be 23 pmol/l, which is considerably lower than most values reported previously. Evidence is presented which suggests that the cross-reactivity of tri-iodothyronine with the antiserum to 3,3′-T2 is an important factor in the measurement of serum concentrations of 3,3′-T2 by radioimmunoassay.

Release of oxytocin during suckling and parturition in the rat

1985 ◽  
Vol 105 (3) ◽  
pp. 339-346 ◽  
Author(s):  
T. Higuchi ◽  
K. Honda ◽  
T. Fukuoka ◽  
H. Negoro ◽  
K. Wakabayashi
Keyword(s):  
Half Life ◽  
General Circulation ◽  
Short Half Life ◽  
Specific Radioimmunoassay ◽  
Highly Sensitive ◽  
Anaesthetized Rats

ABSTRACT A highly sensitive and specific radioimmunoassay (RIA) for oxytocin was developed and used to measure oxytocin concentrations during both suckling and parturition in individual rats. In urethane-anaesthetized rats, the suckling stimuli, provided by ten pups, induced intermittent increases in intramammary pressure of about 10 mmHg. This was associated with a significant (P < 0·01) increase in serum oxytocin levels from 19·5 ± 4·5 (s.e.m., n = 9) to 49·1 ± 7·4 pmol/l (n = 9) in the samples taken within 30 s from the time of the peak in the pressure. These rises in serum oxytocin returned rapidly to the basal levels as expected from the short half-life (1·46 min) of oxytocin in general circulation. On day 22 or 23 of gestation, serum oxytocin levels remained stable until 0–0·5 h before the first fetus was expelled. They then increased significantly (P < 0·01) from 27·6± 4·6 pmol/l (n = 19) in samples taken 0–0·5 h before to 45·1 ± 5·6 pmol/l in samples taken after the expulsion of the first fetus and gradually increased until the last fetus was expelled. Serum oxytocin concentrations then declined but remained higher than those observed before the first fetus had been born until at least 1–1·5 h after the expulsion of the last fetus. Thus, this oxytocin RIA revealed increased concentrations of the hormone in blood during both suckling and parturition in the rat. J. Endocr. (1985) 105, 339–346

The 24-Hour Plasma Thyrotrophin Profile

1972 ◽  
Vol 43 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Y. C. Patel ◽  
F. P. Alford ◽  
H. G. Burger
Keyword(s):  
Peak Concentration ◽  
Plasma Concentrations ◽  
Circadian Variation ◽  
Sampling Technique ◽  
Thyroid Stimulating Hormone ◽  
Specific Radioimmunoassay ◽  
Highly Sensitive ◽  
Tsh Secretion ◽  
The Mean ◽  
Normal Males

1. Daily fluctuations in the plasma concentrations of thyroid stimulating hormone (TSH) and growth hormone (GH) have been studied in six normal males by using a 24 h continuous blood-sampling technique and a highly sensitive and specific radioimmunoassay for TSH. 2. In all subjects the plasma concentrations of the two hormones were increased at night. The TSH concentrations rose approx. 2 h before the onset of sleep but the peak concentration coincided with the GH peak 2 h after the commencement of sleep. In three subjects who slept in the afternoon, additional peaks of GH activity were found and in two cases peaks of TSH activity also occurred. 3. The mean data from all subjects showed a significant circadian variation of TSH secretion with a maximum between 2 a.m. and 4 a.m. and a minimum between 6 p.m. and 8 p.m.

A Highly Sensitive and Specific Radioimmunoassay for Quantitation of Plasma Fluphenazine

1988 ◽  
Vol 77 (3) ◽  
pp. 255-258 ◽  
Author(s):  
Ee Sing Lo ◽  
Mira Fein ◽  
Chyren Hunter ◽  
Raymond F. Suckow ◽  
Thomas B. Cooper
Keyword(s):  
Specific Radioimmunoassay ◽  
Highly Sensitive

Concentrations of atrial natriuretic peptide in plasma and urine of kidney disease patients

1990 ◽  
Vol 36 (9) ◽  
pp. 1650-1653 ◽  
Author(s):  
F Marumo ◽  
H Sakamoto ◽  
K Ando ◽  
T Ishigami ◽  
T Ishigama
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Renal Disease ◽  
Natriuretic Peptide ◽  
Renal Mass ◽  
Fractional Excretion ◽  
Normal Subjects ◽  
Specific Radioimmunoassay ◽  
Highly Sensitive ◽  
Single Nephron ◽  
Atrial Natriuretic

Abstract Concentrations of human atrial natriuretic peptide-like immunoreactivity (hANP-LI) were measured by a highly sensitive and specific radioimmunoassay (Biochem Biophys Res Commun 1986;137:231-6) in normal subjects and in renal disease patients without accompanying congestive heart failure, hypertension, edema, diabetes, or pregnancy. We attempted to clarify whether the hANP-LI concentration in plasma was increased by loss of renal mass. We found no correlation between the hANP-LI concentration in plasma and creatinine clearance (Ccr, 4.6-122.3 mL/min) in patients with renal disease (n = 63, r = -0.196), nor between hANP-LI concentrations in plasma and urine (n = 97, r = -0.207). The fractional excretion of hANP (FEhANP) correlated significantly with Ccr (n = 63, r = 0.520, P less than 0.01) and with FENa (n = 35, r = -0.503, P less than 0.01). Increased FEhANP in patients with chronic renal failure may have resulted because of an increase in single-nephron glomerular filtration rate similar to the FENa increase in these patients. The present data indicate that decreased renal function itself does not increase the concentration of hANP-LI in plasma.

Development and performance of a highly sensitive carboxyl-terminal-specific radioimmunoassay of calcitonin gene-related peptide.

1988 ◽  
Vol 34 (4) ◽  
pp. 655-660 ◽  
Author(s):  
M Zaidi ◽  
S I Girgis ◽  
I MacIntyre
Keyword(s):  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Specific Radioimmunoassay ◽  
Calcitonin Gene ◽  
Highly Sensitive ◽  
Potent Vasodilator ◽  
High Concentrations ◽  
And Performance ◽  
Highly Sensitive Detection ◽  
Development And Validation

Abstract The calcitonin genes encode a small family of peptides: the circulating hormone calcitonin; its flanking peptide, katacalcin; and a third novel peptide, calcitonin gene-related peptide (CGRP). CGRP is a potent vasodilator and a major circulating product from the calcitonin genes; it may be a physiologically important regulator of blood flow in humans. High concentrations of circulating CGRP are found in medullary thyroid carcinoma. We report the development and validation of a highly sensitive (detection limit 500 amol per tube) radioimmunoassay of CGRP involving a high-affinity antibody directed against the carboxyl terminus of the molecule and a highly pure tracer. The assay is precise, robust, and reproducible, and is therefore a potentially useful analytical method for studying the normal and abnormal physiology of this peptide.

scholarly journals Sickle Hemoglobin: A Specific Radioimmunoassay

Blood ◽  
1974 ◽  
Vol 43 (4) ◽  
pp. 607-611 ◽  
Author(s):  
Peter T. Rowley ◽  
Richard A. Doherty ◽  
Cheryl Rosecrans ◽  
Elsa Cernichiari
Keyword(s):  
Gamma Globulin ◽  
Adult Type ◽  
Hemoglobin S ◽  
Hemoglobin A ◽  
Specific Radioimmunoassay ◽  
Highly Sensitive ◽  
Antibody Complex ◽  
Chloramine T ◽  
Antigen Antibody ◽  
Complete Precipitation

Abstract For the quantitation of hemoglobin S, a radioimmunoassay has been developed which is specific and highly sensitive. Hemoglobin S was purified by column chromatography and injected with complete Freund’s adjuvant into goats. Each goat serum was tested for reactivity against hemoglobins A and S by immunodiffusion and by quantitative precipitation. Hemoglobin A reactivity was removed by absorption with hemoglobin A. One serum so treated was specific for hemoglobin S; it reacted negligibly with hemoglobins A or F. Hemoglobin S was labeled with 125I by the chloramine T method. In the radioimmunoassay, complete precipitation of the antigen—antibody complex was insured by the addition of rabbit antigoat gamma globulin. This assay offers reliable and specific quantitation of as little as 1 ng of hemoglobin S. Assuming that 10% of the hemoglobin in fetal blood at 16 wk gestation is of adult type, this assay is capable of detecting the amount of hemoglobin S in 10-7 ml of homozygous hemoglobin S blood. The prenatal diagnosis of sickle cell anemia by radioimmunoassay will require, in addition, a method for demonstrating the absence of hemoglobin A and a safe method for obtaining fetal erythrocytes without significant contamination by maternal erythrocytes.

Enzymatic degradation of somatostatin by rat plasma and hypothalamus

1978 ◽  
Vol 56 (5) ◽  
pp. 840-843 ◽  
Author(s):  
A. Dupont ◽  
G. Alvarado-Urbina ◽  
J. Côté ◽  
F. Labrie
Keyword(s):  
Enzymatic Degradation ◽  
Rat Plasma ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Specific Radioimmunoassay ◽  
Highly Sensitive ◽  
Hypothalamic Tissue ◽  
Inactivation Process ◽  
Enzymatic Nature ◽  
Plasma Activity

A highly sensitive and specific radioimmunoassay for somatostatin has been used to study inactivation of the neurohormone by plasma and hypothalamic peptidase(s). Specificity of the inactivation process was indicated by the absence of interference by addition of luteinizing hormone releasing hormone, thyrotropin-releasing hormone, oxytocin, or substance P. The inactivating ability of hypothalamic tissue and plasma was destroyed by heating and the protease inhibitor benzamidine prevented plasma activity, thus suggesting the enzymatic nature of the processes involved. The present data suggest that the inactivation of somatostatin by hypothalamus and plasma could be an important factor in the regulation of circulating somatostatin levels.

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