scholarly journals Probiotic Supplementation with S. salivarius K12 in Healthy Adults Increases Oral S. salivarius K12 in a Randomized, Double-Blind, Placebo-Controlled Study

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1584-1584
Author(s):  
Jennifer Smilowitz ◽  
Karen Kalanetra ◽  
Anna Meier ◽  
Karina Kurudimov ◽  
Mark Underwood ◽  
...  
Keyword(s):  
16S Rrna ◽  
Amplicon Sequencing ◽  
Healthy Adults ◽  
Oral Microbiome ◽  
Controlled Study ◽  
Placebo Control ◽  
Double Blind ◽  
Double Blind Placebo ◽  
16S Rrna Amplicon Sequencing ◽  
Supplementation Period

Abstract Objectives Streptococcus salivarius (S. salivarius) K12 supplementation in children and adults has been found to reduce the risk of recurrent pharyngitis, tonsillitis, otitis media caused by group A streptococci. The protection of S. salivarius K12 supplementation may in part result from its production of lantibiotic bacteriocins salivaricin A and B. Yet, studies have not reported the effect of supplementation on oral S. salivarius K12 levels or the broader salivary microbiome. The objective of this clinical trial was to determine how supplementation with S. salivarius K12 influences the oral microbiome. Methods In a double-blind, placebo-control, prospective trial, 24 healthy adults were randomized to consume a probiotic powder (PRO) containing 7.8B CFU of L. acidophilus, 8.25B CFU of B. lactis, and 2B CFU of S. salivarius K12 (n = 12) or a placebo-control powder (CON) (n = 12) for fourteen consecutive days. Saliva samples were collected at baseline, at the end of the fourteen-day supplementation period and two weeks post-supplementation. Oral S. salivarius K12 and total bacteria were quantified by QPCR and the oral microbiome was measured using 16s rRNA amplicon sequencing. Results Supplementation with S. salivarius K12 significantly increased salivary S. salivarius K12 by 5 logs compared to baseline for the PRO group (P < 0.0005) and returned to baseline 2-weeks post-supplementation. Compared with the CON group, salivary S. salivarius K12 was 5 logs higher in the PRO group at the end of the supplementation period (P < 0.001). Neither time nor supplementation influenced the oral microbiome. The supplement was well-tolerated. Conclusions Supplementation with a probiotic containing S. salivarius K12 for two weeks significantly increased levels of salivary S. salivarius K12 by 5 logs but had no effect on the overall oral microbiome measured by 16s rRNA amplicon sequencing. Funding Sources Renew Life funded this research but had no part in the analysis or interpretations of the study findings.

scholarly journals Multi-Strain Probiotic Supplementation with a Product Containing Human-Native S. salivarius K12 in Healthy Adults Increases Oral S. salivarius

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4392
Author(s):  
Karina Cernioglo ◽  
Karen M. Kalanetra ◽  
Anna Meier ◽  
Zachery T. Lewis ◽  
Mark A. Underwood ◽  
...  
Keyword(s):  
Controlled Trial ◽  
Amplicon Sequencing ◽  
Healthy Adults ◽  
Oral Microbiome ◽  
Placebo Control ◽  
Upper Respiratory Tract ◽  
Double Blind ◽  
16S Rrna Amplicon Sequencing ◽  
Supplementation Period ◽  
Tract Infections

Streptococcus salivarius (S. salivarius) K12 supplementation has been found to reduce the risk of recurrent upper respiratory tract infections. Yet, studies have not reported the effect of supplementation on oral S. salivarius K12 levels or the salivary microbiome. This clinical trial was designed to determine how supplementation with S. salivarius K12 influences the oral microbiome. In a randomized, double-blind, placebo-controlled trial, 13 healthy adults received a probiotic powder (PRO) containing Lactobacillus acidophilus, Bifidobacterium lactis, and S. salivarius K12 and 12 healthy adults received a placebo-control powder (CON) (n = 12) for 14 consecutive days. Oral S. salivarius K12 and total bacteria were quantified by qPCR and the overall oral microbiome was measured using 16S rRNA amplicon sequencing. Supplementation significantly increased mean salivary S. salivarius K12 levels by 5 logs compared to baseline for the PRO group (p < 0.0005), which returned to baseline 2 weeks post-supplementation. Compared with the CON group, salivary S. salivarius K12 was 5 logs higher in the PRO group at the end of the supplementation period (p < 0.001). Neither time nor supplementation influenced the overall oral microbiome. Supplementation with a probiotic cocktail containing S. salivarius K12 for two weeks significantly increased levels of salivary S. salivarius K12.

scholarly journals 902. A Randomized, Double-Blind, Placebo-Controlled Study to Evaluate the Efficacy of a Single Immunization of Ad26.RSV.preF against RSV Infection in a Viral Challenge Model in Healthy Adults

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S27-S28 ◽  
Author(s):  
John DeVincenzo ◽  
Efi Gymnopoulou ◽  
Els De Paepe ◽  
Bryan Murray ◽  
Arangassery Rosemary Bastian ◽  
...  
Keyword(s):  
Disease Severity ◽  
Healthy Adults ◽  
Controlled Study ◽  
Clinical Strain ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Qrt Pcr ◽  
Rsv Infection ◽  
First Time ◽  
Over Time

Abstract Background Despite the high disease burden of RSV in older adults and children, there is currently no approved vaccine. Ad26.RSV.preF, an experimental RSV vaccine, has demonstrated immunogenicity and tolerability in first-in-human clinical studies. The aim of this study was to assess the potential of the Ad26.RSV.preF vaccine to protect against RSV infection and disease in an established RSV human challenge model, used for the first time to evaluate a vaccine. Methods We conducted a randomized, double-blind, placebo-controlled, human challenge study (NCT03334695). Healthy adults received 1 × 1011 vp Ad26.RSV.preF vaccine (active) or placebo (pbo) intramuscularly. After 28 days, volunteers were challenged intranasally with a low-passage clinical strain of RSV-A (0.8 mL of Memphis 37b) and then quarantined for 12 days. Nasal washes were collected twice daily throughout quarantine, starting 2 days post-challenge (viral load [VL] by qRT-PCR and quantitative cultures). Disease severity was recorded thrice daily using symptom diary cards. Results Fifty-three volunteers (active, n = 27; pbo, n = 26) were challenged with RSV-A. Quantitative viral assessments were consistently lower in active than pbo. The primary endpoint of the study was met: the area under the curve (AUC) for RSV VL over time (via qRT-PCR) was significantly lower in active pbo (P = 0.012). Median peak VL was lower for active (0 log10 copies/mL) than pbo (5.4 log10 copies/mL). Median AUC for RSV VL over time (quantitative culture) was lower for active than pbo (0 vs. 109, P = 0.002). Disease severity was lower for active than pbo, with a median AUC total symptom score of 35 (active) vs. 167 (pbo) (P = 0.002). Overall, RSV infection (defined by qRT-PCR alone or combined with symptoms) and disease severity over time were lower in active vs. pbo. Conclusion RSV infections, VL, and RSV disease severity were consistently lower in healthy adults receiving Ad26.RSV.preF vs. placebo, demonstrating promising protection from RSV infection and disease. This was the first time that antiviral prevention was observed against RSV after active immunization. Ad26.RSV.preF warrants further evaluation in field trials for efficacy against natural RSV infections in populations considered at risk of severe RSV disease. Disclosures All Authors: No reported Disclosures.

scholarly journals Effect of Supplementation of a Whey Peptide Rich in Tryptophan-Tyrosine-Related Peptides on Cognitive Performance in Healthy Adults: A Randomized, Double-Blind, Placebo-Controlled Study

Nutrients ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 899 ◽  
Author(s):  
Masahiro Kita ◽  
Kuniaki Obara ◽  
Sumio Kondo ◽  
Satoshi Umeda ◽  
Yasuhisa Ano
Keyword(s):  
Cognitive Function ◽  
Cognitive Decline ◽  
Cognitive Performance ◽  
Healthy Adults ◽  
Controlled Study ◽  
Peptide Group ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Cognitive Improvement ◽  
Subjective Fatigue

Background: Previous epidemiological and clinical studies have shown that dairy products have beneficial effects on cognitive decline and dementia. Enzymatic digestion of whey protein produces a whey peptide rich in tryptophan-tyrosine-related peptides which improve cognitive performance in mice. We evaluated the effects of whey peptides on cognitive functions in healthy adults in a randomized, double-blind, placebo-controlled design. Methods: 101 healthy adults (45 to 64 years), with a self-awareness of cognitive decline received either whey peptide or placebo supplements for 12 weeks. Changes in cognitive function were assessed using neuropsychological tests at 6 and 12 weeks after the start of supplementation. Results: Verbal fluency test (VFT) score changes tended to be higher in the whey peptide group compared with the placebo at 12 weeks. Subgroup analysis classified by the degree of subjective fatigue showed that changes in the VFT as well as the Stroop and subjective memory function tests between baseline and 6 weeks of intervention were significantly better in subjects with high-level fatigue from the whey peptide group as compared to the placebo group. Conclusions: Intake of whey peptide might improve cognitive function in healthy middle- and older-aged adults with high subjective fatigue levels. Further studies will elucidate the relationship among cognitive improvement, whey peptides, and psychological fatigue.

Comparison of the Diuretic Effects of Single Dose Frusemide/Triamterene and Hydrochlorothiazide/Amiloride in Healthy Adults

1986 ◽  
Vol 14 (4) ◽  
pp. 188-192
Author(s):  
A Kohvakka ◽  
E Hussi
Keyword(s):  
Single Dose ◽  
Fixed Combination ◽  
Healthy Adults ◽  
Controlled Study ◽  
Urinary Volume ◽  
Double Blind ◽  
Double Blind Placebo

The diuretic and potassium- and magnesium-conserving action of a single dose of frusemide/triamterene, F+TRI (40 mg/50 mg), and hydrochlorothiazide/amiloride, HCT+AMI (50 mg/5 mg), fixed combination preparations were compared in ten healthy adults in a randomised double-blind placebo-controlled study. Urinary volume during 24 hours was significantly higher after HCT+AMI ingestion than after F+TRI. The urine and sodium excretions caused by HCT+AMI lasted markedly longer and were smoother than the short and somewhat unpleasant effects of F+ TRI. HCT+AMI also spared potassium and magnesium relatively more effectively than F + TRI.

scholarly journals Kiwifruit-derived supplements increase stool frequency in healthy adults: a randomized, double-blind, placebo-controlled study

2015 ◽  
Vol 35 (5) ◽  
pp. 401-408 ◽  
Author(s):  
Juliet Ansell ◽  
Christine A. Butts ◽  
Gunaranjan Paturi ◽  
Sarah L. Eady ◽  
Alison J. Wallace ◽  
...  
Keyword(s):  
Healthy Adults ◽  
Stool Frequency ◽  
Controlled Study ◽  
Double Blind ◽  
Double Blind Placebo
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