scholarly journals Stinging Nettle (Urtica simensis) as Potential Resource of Vitamin E

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1815-1815
Author(s):  
Tibebeselassie Keflie

Abstract Objectives The aim of the current study was to assay the content of vitamin E in stinging nettle (Urtica simensis) Methods Urtica simensis type of stinging nettle is an indigenous wild plant which is widely growing in different parts of Ethiopia. Samples of leaves were collected from Chacha, one of the central highlands in Ethiopia and portioned into sun dried, shade dried and lyophilized groups. For comparison, samples of leaves were also taken from spinach. Vitamin E family such as tocopherols ((α, β, γ, and λ) and tocotrienols (α, β, γ, and λ) were determined using high performance liquid chromatography (HPLC) at department of Food Biofunctionality, University of Hohenheim, Stuttgart, Germany. Results The results showed that the total tocols of stinging nettle in sun-dried, shade dried, and lyophilized groups were 14.1 ± 1.1 mg, 13.8 ± 1.1 mg and 16.9 ± 1.2 mg per 100 g, respectively. In spinach, this value was 3.04 ± 0.7 mg/100 g. Of all vitamin E family, α- tocopherol was the maximum and identified in shade dried group (16.5 ± 1.2 mg/100 g). As compared to stinging nettle, spinach contained very small amount of α- tocopherol (1.7 ± 0.5 mg/100 g). Conclusions In conclusion, Urtica simensis type of stinging nettle contains considerable amount of tocols and can serve as potential resource of vitamin E. Further research is warranted on the nutritional and medicinal values of Urtica simensis stinging nettle. Funding Sources None.

2017 ◽  
Vol 54 (3) ◽  
pp. 294
Author(s):  
Hari Ramakrishnan K. ◽  
Janaky Ranjithkumar

Vitamin E, the fat soluble vitamin is present naturally in some foods and added in food supplements, nutraceuticals etc due to its vital biological function as an antioxidant. Various methods are available for the analysis of vitamin E. Especially High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are exclusively used for the quantitative evaluation of vitamin E, which has also identified the four different isomeric forms of this vitamin. The rate of losses of this vitamin during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive procedure for their separations. Though effective, HPLC instrument is expensive and comparatively cumbersome. In this prospective, the study was to evaluate the usefulness of High Performance Thin Layer Chromatography (HPTLC) in the analysis of vitamin E. There are methods available using Thin Layer Chromatography for its analysis, but they are not sensitive enough to identify the isomeric forms of vitamin E. In this HPTLC method, the different isomeric forms of vitamin E - α, β, γ and δ were identified. This technique shall be considered as an alternative to the other methods such as HPLC and GC.


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