Stability-Indicating Liquid Chromatographic Methods with Photodiode Array Detection and Light Scattering Detection for Simultaneous Determination of Candesartan and Hydrochlorothiazide

2017 ◽  
Vol 56 (2) ◽  
pp. 99-107 ◽  
Author(s):  
Marta de Diego ◽  
Ricardo Godoy ◽  
Sigrid Mennickent ◽  
Carola Vergara ◽  
Daniel Miranda ◽  
...  
Keyword(s):  
Light Scattering ◽  
Simultaneous Determination ◽  
Photodiode Array Detection ◽  
Stability Indicating ◽  
Chromatographic Methods ◽  
Light Scattering Detection ◽  
Photodiode Array ◽  
Array Detection ◽  
Liquid Chromatographic

Validated Stability-Indicating Methods for the Simultaneous Determination of Amiloride Hydrochloride, Atenolol, and Chlorthalidone Using HPTLC and HPLC with Photodiode Array Detector

2013 ◽  
Vol 96 (2) ◽  
pp. 313-323 ◽  
Author(s):  
Rasha M Youssef ◽  
Hadir M Maher ◽  
Eman I El-Kimary ◽  
Ekram M Hassan ◽  
Magda H Barary
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Pharmaceutical Preparations ◽  
Array Detector ◽  
Photodiode Array Detection ◽  
Stability Indicating ◽  
Chromatographic Methods ◽  
Photodiode Array ◽  
Amiloride Hydrochloride

Abstract Two stability-indicating chromatographic methods are described for simultaneous determination of amiloride hydrochloride (AMI), atenolol (ATE), and chlorthalidone (CHL) in combined dosage forms. The first method was based on HPTLC separation of the three drugs followed by densitometric measurements of their bands at 274 nm. The separation was carried out on Merck HPTLC silica gel 60F254 aluminum sheets using chloroform–methanol–ammonia 27%, w/w (9 + 2 + 0.3, v/v/v) mobile phase. Analysis data was used for the linear regression graph in the range of 0.1–0.5, 0.8–5.0, and 0.3–1.5 μg/band for AMI, ATE, and CHL, respectively. The second method was based on an RP-HPLC separation of the cited drugs performed on an RP stainless steel C18 analytical column (250 × 4.6 mm id) with a gradient elution system of methanol and 0.05 M aqueous phosphate buffer adjusted to pH 4 as the mobile phase, at the flow rate of 1.0 mL/min. Quantitation was achieved with photodiode array detection at 275 nm for AMI and 225 nm for ATE and CHL. The calibration graphs for each drug were rectilinear in the range of 2–50, 25–150, and 2–100 μg/mL for AMI, ATE, and CHL, respectively. The proposed chromatographic methods were successfully applied for determination of the investigated drugs in pharmaceutical preparations. Both methods were validated in compliance with International Conference on Harmonization guidelines in terms of linearity, accuracy, precision, robustness, LOD, and LOQ.

scholarly journals Liquid Chromatographic Determination of Five Benzoylurea Insecticides in Fruit and Vegetables

1998 ◽  
Vol 81 (5) ◽  
pp. 1037-1042 ◽  
Author(s):  
Miguel Gamón ◽  
Adolfo Saez ◽  
Rosa Pelegrí ◽  
Inmaculada Peris ◽  
Josá G De La Cuadra ◽  
...  
Keyword(s):  
Solid Phase ◽  
Chromatographic Determination ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Array Detection ◽  
Liquid Chromatographic Determination ◽  
Benzoylurea Insecticides ◽  
Vegetables And Fruits ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method was developed to determine 5 benzoyl ureas—diflubenzuron, hexaflumuron, teflubenzuron, flufenozuron, and lufenuron—in peppers, tomatoes, eggplants, cucumbers, and oranges. Preparation of samples involve extraction with acetone and partitioning into dichloromethane-petroleum ether. A portion of this extract is cleaned up with a solid-phase extraction aminopropyl disposable column. With LC analysis using an RP-8-DB microbore column, acetonitrilewater (70 + 30, v/v) as mobile phase, and photodiode array detection at 254 nm, recovery and repeatability data were collected for the 5 benzoylureas on 4 vegetables and citrus in the range 0.04-2.0 mg/kg. Validated limits of detection and quantitation were 0.01 and 0.04 mg/kg, respectively. The method is reliable for routine analysis of vegetables and fruits

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