Neutralizing Antibody Responses to Homologous and Heterologous H1 and H3 Influenza A Strains After Vaccination With Inactivated Trivalent Influenza Vaccine Vary With Age and Prior-year Vaccination

SUMMARYFifty volunteers, treated with an inactivated trivalent influenza vaccine containing A/Bangkok/1/79 (H3N2), A/Brazil/11/78 (H1N1) and B/Singapore/222/79 virus, were subdivided according to the estimated first exposure to influenza in their lifetime (priming) and the presence of antibodies against the vaccine components in the pre-vaccination sera. The isotypic antibody response (IgG, IgA, IgM) was determined by means of an antibody capture haemadsorption immunosorbent technique. For all three vaccine components, previously seropositive subjects produced antibodies of the IgG- and IgA-class more frequently than previously seronegative persons. Subjects primed to one of the influenza A subtypes showed more IgG and IgA responses in comparison with those unprimed (prime-effect). In contrast, IgM antibodies occurred in only 19 and 11% of primed, but in 59 and 54% of unprimed subjects, for A (H3N2) and A (HlNl), respectively. The incidence of IgM titre rises was not influenced by the prevaccination state. However, the mean magnitude of anti-A(H1N1)-IgM titre rises was greater in those previously seronegative. The concepts of primary and reinfection and of ‘original antigenic sin’ are discussed, and it is suggested that age and, if possible, serological state prior to antigen-exposure should be taken into account when studying isotypic antibody responses after influenza infection or vaccination.

Download Full-text

Comparison of the Hemagglutination-Inhibiting and Neutralizing Antibody Responses of Volunteers Given 400 Chick Cell-Agglutinating Units of Influenza A/New Jersey/76 Split-Virus Vaccine

The Journal of Infectious Diseases ◽

10.1093/infdis/136.supplement_3.s472 ◽

1977 ◽

Vol 136 (Supplement 3) ◽

pp. S472-S474 ◽

Cited By ~ 5

Author(s):

J. Massicot ◽

B. R. Murphy

Keyword(s):

New Jersey ◽

Virus Vaccine ◽

Influenza A ◽

Neutralizing Antibody ◽

Antibody Responses

Download Full-text

Vaccine effectiveness for influenza in the elderly in welfare nursing homes during an influenza A (H3N2) epidemic

Epidemiology and Infection ◽

10.1017/s0950268899004410 ◽

2000 ◽

Vol 125 (2) ◽

pp. 393-397 ◽

Cited By ~ 10

Author(s):

Y. DEGUCHI ◽

Y. TAKASUGI ◽

K. NISHIMURA

Keyword(s):

Nursing Homes ◽

Influenza Vaccine ◽

Influenza A ◽

Hospital Admissions ◽

Influenza Virus Infection ◽

Severe Infection ◽

The Elderly ◽

Trivalent Influenza Vaccine ◽

Homes For The Elderly ◽

Vaccine Effect

Influenza vaccine effect on the occurrence and severity of influenza virus infection in a population residing in nursing homes for the elderly was studied as a cohort study during an influenza A (H3N2) epidemic in Japan. Of 22462 individuals living in 301 welfare nursing homes, 10739 voluntarily received inactivated, sub-unit trivalent influenza vaccine in a programme supported by the Osaka Prefectural Government. There were statistically significantly fewer cases of influenza, hospital admissions due to severe infection, and deaths due to influenza in the vaccinated cohort compared to the unvaccinated controls. No serious adverse reactions to vaccination were recorded. Thus influenza vaccination is effective for preventing influenza disease in persons aged 65 years and over, and should be an integral part of the care of this population residing in nursing homes.

Download Full-text

Adjuvanted Influenza Vaccines Elicits Higher Antibody Responses against the A(H3N2) Subtype than Non-Adjuvanted Vaccines

Vaccines ◽

10.3390/vaccines8040704 ◽

2020 ◽

Vol 8 (4) ◽

pp. 704

Author(s):

Laura Sánchez de Prada ◽

Iván Sanz Muñoz ◽

Javier Castrodeza Sanz ◽

Raúl Ortiz de Lejarazu Leonardo ◽

José María Eiros Bouza

Keyword(s):

Influenza Vaccine ◽

Influenza A ◽

Seasonal Influenza ◽

The Elderly ◽

Antibody Responses ◽

Serological Response ◽

Serum Samples ◽

H3n2 Subtype ◽

Serological Studies ◽

Vaccination Campaigns

Background: vaccination is the best approach to prevent influenza infections so far. Serological studies on the effect of different vaccine types are important to address vaccination campaigns and protect our population. In our study, we compared the serological response against influenza A subtypes using the non-adjuvanted influenza vaccine (NAIV) in adults and the elderly and the adjuvanted influenza vaccine (AIV) in the elderly. Methods: We performed a retrospective analysis by hemagglutination inhibition assay (HI) of serum samples right before and 28 days after seasonal influenza vaccination during the 1996–2017 seasons. Conclusions: The AIV presents better performance against the A(H3N2) subtype in the elderly whereas the NAIV induces a better response against A(H1N1)pdm09 in the same group.

Download Full-text

Immunogenicity of a Monovalent Pandemic Influenza A H1N1 Virus Vaccine with or without Prior Seasonal Influenza Vaccine Administration

Clinical and Vaccine Immunology ◽

10.1128/cvi.00077-12 ◽

2012 ◽

Vol 19 (10) ◽

pp. 1690-1692 ◽

Cited By ~ 1

Author(s):

Hidetoshi Igari ◽

Akira Watanabe ◽

Shunsuke Segawa ◽

Akiko Suzuki ◽

Mariko Watanabe ◽

...

Keyword(s):

Influenza Vaccine ◽

Pandemic Influenza ◽

Influenza A ◽

H1n1 Virus ◽

Geometric Mean ◽

Vaccine Administration ◽

Trivalent Influenza Vaccine ◽

Influenza A H1n1 ◽

Significant Difference ◽

Antibody Titers

ABSTRACTThe immunogenicity of pandemic influenza A H1N1 virus (A/H1pdm) vaccine might be modified by prior seasonal trivalent influenza vaccine (sTIV) administration. We conducted a retrospective analysis of immunogenicity of 243 health care workers (number of sTIV-positive [sTIV+] subjects, 216; number of sTIV−subjects, 27) by hemagglutination inhibition. There was no significant difference in the ratios of antibody titers of ≥40 (41.2% versus 48.1%;P= 0.49) and fold increases in geometric mean titer (3.8 versus 4.5;P= 0.37). sTIV injected 7 to 10 days prior to A/H1pdm vaccine administration did not interfere with the immunogenicity of the latter.

Download Full-text

Antibody responses to influenza a H1N1 vaccine compared to the circulating strain in influenza vaccine recipients during the 2013/2014 season in North America

Journal of Clinical Virology ◽

10.1016/j.jcv.2016.08.296 ◽

2016 ◽

Vol 83 ◽

pp. 56-60 ◽

Cited By ~ 1

Author(s):

Michelle A. Barron ◽

Daniel N. Frank ◽

David Claypool ◽

Diana Ir ◽

Mariangeli F. Ning ◽

...

Keyword(s):

North America ◽

Influenza Vaccine ◽

Influenza A ◽

Antibody Responses ◽

H1n1 Vaccine ◽

Influenza A H1n1

Download Full-text

Neutralizing Antibody Responses to Antigenically Drifted Influenza A(H3N2) Viruses among Children and Adolescents following 2014-2015 Inactivated and Live Attenuated Influenza Vaccination

Clinical and Vaccine Immunology ◽

10.1128/cvi.00297-16 ◽

2016 ◽

Vol 23 (10) ◽

pp. 831-839 ◽

Cited By ~ 15

Author(s):

Min Z. Levine ◽

Judith M. Martin ◽

F. Liaini Gross ◽

Stacie Jefferson ◽

Kelly Stefano Cole ◽

...

Keyword(s):

Influenza Vaccine ◽

Children And Adolescents ◽

Influenza A ◽

Influenza Season ◽

Health Care Facilities ◽

Antibody Responses ◽

Vaccine Component ◽

Vaccine Type ◽

Antibody Titers ◽

Reactive Antibody

ABSTRACTHuman influenza A(H3N2) viruses that predominated during the moderately severe 2014-2015 influenza season differed antigenically from the vaccine component, resulting in reduced vaccine effectiveness (VE). To examine antibody responses to 2014-2015 inactivated influenza vaccine (IIV) and live-attenuated influenza vaccine (LAIV) among children and adolescents, we collected sera before and after vaccination from 150 children aged 3 to 17 years enrolled at health care facilities. Hemagglutination inhibition (HI) assays were used to assess the antibody responses to vaccine strains. We evaluated cross-reactive antibody responses against two representative A(H3N2) viruses that had antigenically drifted from the A(H3N2) vaccine component using microneutralization (MN) assays. Postvaccination antibody titers to drifted A(H3N2) viruses were higher following receipt of IIV (MN geometric mean titers [GMTs], 63 to 68; 38 to 45% achieved seroconversion) versus LAIV (MN GMT, 22; only 3 to 5% achieved seroconversion). In 9- to 17-year-olds, the highest MN titers were observed among IIV-vaccinated individuals who had received LAIV in the previous season. Among all IIV recipients aged 3 to 17 years, the strongest predictor of antibody responses to the drifted viruses was the prevaccination titers to the vaccine strain. The results of our study suggest that in an antigenically drifted influenza season, vaccination still induced cross-reactive antibody responses to drifted circulating A(H3N2) viruses, although higher antibody titers may be required for protection. Antibody responses to drifted A(H3N2) viruses following vaccination were influenced by multiple factors, including vaccine type and preexisting immunity from prior exposure.

Download Full-text