A Method for the Rapid Detection of Acute Iron Toxicity

Abstract In acute iron poisoning, it is necessary for rational management to know whether the plasma iron exceeds the transferrin-binding capacity. A method for the rapid determination of iron in plasma is described whereby this essential information may be obtained in a few minutes. This test will help to differentiate simple iron ingestion from acute iron toxicity so the clinician may have a firmer basis upon which to decide whether chelate therapy is indicated.

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Smartphone-based molecularly imprinted sensors for rapid detection of thiamethoxam residues and applications

PLoS ONE ◽

10.1371/journal.pone.0258508 ◽

2021 ◽

Vol 16 (11) ◽

pp. e0258508

Author(s):

Sihua Peng ◽

Aqiang Wang ◽

Yuyang Lian ◽

Xi Zhang ◽

Bei Zeng ◽

...

Keyword(s):

Pesticide Residues ◽

Rapid Detection ◽

Rapid Determination ◽

Detection System ◽

Residue Analysis ◽

Differential Pulse ◽

Detection Technology ◽

Screen Printed Carbon Electrode ◽

Relative Standard

In order to achieve rapid detection of thiamethoxam residues in mango, cowpea and water, this study modified the screen printed carbon electrode (SPCE) to make a specific molecular imprinting sensor (Thiamethoxam-MIP/Au/rGO/SPCE) for thiamethoxam. An integrated smartphone platform was also built for thiamethoxam residue analysis. The performance of the complete system was analyzed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The system was then applied for the rapid determination of thiamethoxam residues in water, mango and cowpea samples. The results showed that the molecular sensor showed good linearity in the range 0.5–3.0 μmol/L of thiamethoxam. The detection limit of thiamethoxam was 0.5 μmol/L. Moreover, the sensor had good reproducibility and anti-interference performance. The average recovery rates of the pesticide residues in water, mango and cowpea samples were in the range of 90–110% with relative standard deviations < 5%. The rapid detection system for thiamethoxam residue constructed in this study was simple, reliable, reproducible and had strong anti-interference. It has broad application prospects in the field detection of thiamethoxam residue, and serves as a valuable reference for the further development of rapid detection technology of pesticide residues in the field of environment and food safety.

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Rapid Determination of Sucrose in Fruit Juices: A New Sensitive Carbon Nanotube Paste Osmium-Polymer Mediated Biosensor

Journal of Food Research ◽

10.5539/jfr.v3n4p101 ◽

2014 ◽

Vol 3 (4) ◽

pp. 101 ◽

Cited By ~ 5

Author(s):

Riccarda Antiochia ◽

Lo Gorton ◽

Luisa Mannina

Keyword(s):

Carbon Nanotube ◽

Rapid Detection ◽

Fruit Juice ◽

Rapid Determination ◽

Fast Response ◽

Fruit Juices ◽

Specific Detection ◽

Single Walled Carbon Nanotube ◽

Fructose Dehydrogenase

The aim of the present work was the development of a novel amperometric biosensor for rapid detection of sucrose in fruit juices samples. Two enzymes, invertase and fructose dehydrogenase (FDH), were immobilized onto a single-walled carbon nanotube paste (SWCNTP) electrode by wiring with a highly original osmium-polymer hydrogel. A second biosensor, for fructose only, was constructed containing inactive invertase and used for signal subtraction. The biosensor exhibits a detection limit for sucrose of 2 mM, linearity up to 5 mM, good sensitivity of 1.98 mA cm-2 mM, good reproducibility (RSD = 2.5%), fast response time (8s) and a stability of 4 months if kept under wet conditions at 4 °C. The biosensor was successively tested for specific detection of sucrose and fructose in several commercial fruit juice samples and the results were compared with those obtained with a commercial spectrophotometric enzymatic kit.

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In situ synthesis of fluorescent copper nanoclusters for rapid detection of ascorbic acid in biological samples

Analytical Methods ◽

10.1039/c9ay01627a ◽

2019 ◽

Vol 11 (36) ◽

pp. 4580-4585 ◽

Cited By ~ 5

Author(s):

Xuan Wang ◽

Caicheng Long ◽

Zixin Jiang ◽

Taiping Qing ◽

Kaiwu Zhang ◽

...

Keyword(s):

Ascorbic Acid ◽

Biological Samples ◽

Rapid Detection ◽

Rapid Determination ◽

In Situ Synthesis ◽

Copper Nanoclusters ◽

Red Emission

Schematic presentation of the in situ synthesis of fluorescent copper nanoclusters for rapid determination of ascorbic acid is provided. After addition of ascorbic acid, copper(ii) is reduced to copper(0) and then clustered on dsDNA, thus producing red-emission CuNCs.

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BOCILLIN FL, a Sensitive and Commercially Available Reagent for Detection of Penicillin-Binding Proteins

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.43.5.1124 ◽

1999 ◽

Vol 43 (5) ◽

pp. 1124-1128 ◽

Cited By ~ 196

Author(s):

Genshi Zhao ◽

Timothy I. Meier ◽

Steven D. Kahl ◽

Kyle R. Gee ◽

Larry C. Blaszczak

Keyword(s):

Escherichia Coli ◽

Pseudomonas Aeruginosa ◽

Rapid Detection ◽

Binding Proteins ◽

Uv Light ◽

Rapid Determination ◽

Penicillin G ◽

Direct Evaluation ◽

Penicillin Binding Proteins

ABSTRACT We describe a new, sensitive, rapid, and nonradioactive method involving the use of the commercially available BOCILLIN FL, a fluorescent penicillin, as a labeling reagent for the detection and study of penicillin-binding proteins (PBPs). This method allowed rapid detection of 30 ng of a purified PBP protein under UV light and of 2 to 4 ng of the protein with the aid of a FluorImager. This method also allowed rapid determination of the PBP profiles of Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pneumoniae. The PBP profiles obtained are virtually identical to those reported previously with 3H-,14C-, or 125I-labeled penicillin. Using this method enabled us to determine the 50% inhibitory concentrations of the penicillin-sensitive and -resistant PBP2x proteins of S. pneumoniae for penicillin G, thereby allowing a direct evaluation of their relative affinities for penicillin G. Finally, this method also allowed us to compare relative affinities of a PBP2x protein for different β-lactam antibiotics with the aid of fluorescence polarization technology and to monitor a PBP2x protein during purification.

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Weak Beam Imaging and Diffraction Studies of Stacking Faults in Silicon

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100092918 ◽

1976 ◽

Vol 34 ◽

pp. 590-591

Author(s):

T. Y. Tan ◽

W. K. Tice

Keyword(s):

Fast Neutron ◽

Rapid Determination ◽

Stacking Faults ◽

Imaging Method ◽

Large Reduction ◽

Dislocation Loops ◽

Fringe Spacing ◽

Weak Beam ◽

Kinematical Theory

In studying ion implanted semiconductors and fast neutron irradiated metals, the need for characterizing small dislocation loops having diameters of a few hundred angstrom units usually arises. The weak beam imaging method is a powerful technique for analyzing these loops. Because of the large reduction in stacking fault (SF) fringe spacing at large sg, this method allows for a rapid determination of whether the loop is faulted, and, hence, whether it is a perfect or a Frank partial loop. This method was first used by Bicknell to image small faulted loops in boron implanted silicon. He explained the fringe spacing by kinematical theory, i.e., ≃l/(Sg) in the fault fringe in depth oscillation. The fault image contrast formation mechanism is, however, really more complicated.

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