In situ synthesis of fluorescent copper nanoclusters for rapid detection of ascorbic acid in biological samples

2019 ◽  
Vol 11 (36) ◽  
pp. 4580-4585 ◽  
Author(s):  
Xuan Wang ◽  
Caicheng Long ◽  
Zixin Jiang ◽  
Taiping Qing ◽  
Kaiwu Zhang ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Biological Samples ◽  
Rapid Detection ◽  
Rapid Determination ◽  
In Situ Synthesis ◽  
Copper Nanoclusters ◽  
Red Emission

Schematic presentation of the in situ synthesis of fluorescent copper nanoclusters for rapid determination of ascorbic acid is provided. After addition of ascorbic acid, copper(ii) is reduced to copper(0) and then clustered on dsDNA, thus producing red-emission CuNCs.

Gold nanoparticles as a colorimetric probe for the determination of N-acetyl-l-cysteine in biological samples and pharmaceutical formulations

2015 ◽  
Vol 7 (4) ◽  
pp. 1478-1483 ◽  
Author(s):  
Saadat Rastegarzadeh ◽  
Fatemeh Hashemi
Keyword(s):  
Gold Nanoparticles ◽  
Ascorbic Acid ◽  
Biological Samples ◽  
Pharmaceutical Formulations ◽  
Reducing Agent ◽  
Spectrophotometric Detection ◽  
Colorimetric Probe

In this study, gold nanoparticles (AuNPs) have been synthesised using ascorbic acid as a reducing agent and employed in the design of an in situ colorimetric probe for the spectrophotometric detection of N-acetyl-l-cysteine (NAC).

Rapid determination of glutamine in biological samples by high-performance liquid chromatography

1993 ◽  
Vol 218 (2) ◽  
pp. 159-168 ◽  
Author(s):  
Tom Teerlink ◽  
Marcel W.T. Hennekes ◽  
Paul A.M. van Leeuwen ◽  
Alexander Houdijk
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Biological Samples ◽  
High Performance ◽  
Rapid Determination

Accurate quantitative determination of heavy metals in oily soil by laser induced breakdown spectroscopy (LIBS) combined with interval partial least squares (IPLS)

2019 ◽  
Vol 11 (29) ◽  
pp. 3657-3664 ◽  
Author(s):  
Yu Ding ◽  
Guiyu Xia ◽  
Huiwen Ji ◽  
Xiong Xiong
Keyword(s):  
Heavy Metals ◽  
Rapid Detection ◽  
Detection Method ◽  
Pollution Assessment ◽  
Laser Induced Breakdown Spectroscopy ◽  
Oily Soil ◽  
Breakdown Spectroscopy ◽  
Laser Induced Breakdown

A rapid detection method for heavy metals in oily soil is needed to provide accurate data support for in situ soil pollution assessment and restoration.

scholarly journals Rapid Determination of Lysine in Biological Samples by Isocratic Liquid Chromatography

1999 ◽  
Vol 82 (4) ◽  
pp. 809-813 ◽  
Author(s):  
Mamun M Or-Rashid ◽  
Ryoji Onodera ◽  
Shaila Wadud ◽  
Mohamed-Emad A Nasser ◽  
Mohammad R Amin
Keyword(s):  
Liquid Chromatography ◽  
Biological Samples ◽  
Limit Of Detection ◽  
Rapid Determination ◽  
Rumen Fluid ◽  
Uv Detector ◽  
Rumen Microorganisms ◽  
Microbial Nitrogen ◽  
Limit Of Quantitation

Abstract A simple, rapid, and sensitive method was developed for detection and quantitation of lysine (Lys) in various biological samples by isocratic liquid chromatography (LC). Samples containing Lys and other amino acids were derivatized with 9-fluorenylmethyl chloroformate (FMOC-CI). The mobile phase used for isocratic elution was 50 mmol/L sodium acetate buffer (pH 4.20)-acetonitrile (43 + 57, v/v). Lys was detected with a UV detector at 265 nm. The derivatized Lys eluted from a LiChrospher 100 RP-18 (150× 4.0 mm id) column at a retention time of 5.6 min. The limit of detection was 0.73 μmol/L (signal-to-noise [S/N] ratio, 3:1), and the limit of quantitation was 2.37 μmol/L (S/N ratio, 10:1). Lys recoveries from fortified biological samples were >97.5%. Average Lys contents found in rumen fluid samples collected before the morning feeding and at 2.0,4.0, and 6.0 h after feeding were 4.26,3.34,3.58, and 3.82 μmol/L, respectively. The hydrolysate of a sample of mixed rumen microorganisms collected before the morning feeding was determined to contain 1.372 μmol/mg microbial nitrogen in the form of Lys. The Lys concentrations of human plasma, goat plasma, human urine, and goat urine were 140.0, 102.0,58.0, and 32.0 μmol/L, respectively.

scholarly journals Rapid determination of quaternary protein structures in complex biological samples

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Simon Hauri ◽  
Hamed Khakzad ◽  
Lotta Happonen ◽  
Johan Teleman ◽  
Johan Malmström ◽  
...  
Keyword(s):  
Biological Samples ◽  
Rapid Determination ◽  
Protein Structures
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