Automated Fluorometric Method For The Determination of Serum Calcium

1969 ◽  
Vol 15 (9) ◽  
pp. 870-878 ◽  
Author(s):  
Benjamin Fingerhut ◽  
Alfred Poock ◽  
Henry Miller
Keyword(s):  
Standard Deviation ◽  
Serum Calcium ◽  
Fluorometric Method ◽  
Good Agreement

Abstract An automated fluorometric procedure for serum calcium has been developed which can be used for a wider variety of pathologic serums than the automated fluorometric and colorimetric technics generally being used at present. The method is based upon measurement of the fluorescence produced by calcein with calcium in a serum dialysate. Results are in good agreement with the Clark-Collip procedure. Recovery experiments averaged 98-101%. The standard deviation for duplicate determinations averaged ±0.20 mg/100 ml.

An Automated Method for the Determination of Serum Calcium with Glyoxal Bis (2-Hydroxyanil)

1967 ◽  
Vol 13 (6) ◽  
pp. 515-520 ◽  
Author(s):  
Genevieve Farese ◽  
Janice L Schmidt ◽  
Milton Mager
Keyword(s):  
Serum Calcium ◽  
Automated Analysis ◽  
Automated Method ◽  
Good Agreement

Abstract A completely automated analysis is described for the determination of serum calcium with glyoxal bis (2-hydroxyanil) solution (GBHA). The method is simple and precise, and the data obtained are in good agreement with results obtained by the manual GBHA procedure.

Automated Method for the Analysis of Riboflavin in Milk, with Application to Other Selected Foods

1974 ◽  
Vol 57 (5) ◽  
pp. 1085-1088
Author(s):  
James R Kirk
Keyword(s):  
Standard Deviation ◽  
Continuous Flow ◽  
Quantitative Method ◽  
Green Fluorescence ◽  
Internal Standards ◽  
Automated Method ◽  
Sodium Hydrosulfite ◽  
Automated Technique ◽  
Good Agreement

Abstract A continuous flow automated technique was developed for the determination of riboflavin in milk. The determination is based on the measurement of the natural yellow-green fluorescence of riboflavin at an excitation of 436 nm and emission of 510 nm. Blank values are determined for each sample after sodium hydrosulfite reduction of the riboflavin. Mean recovery and standard deviation for riboflavin in milk determined by the continuous flow procedure using internal standards were 9 7% and ± 2.42%, respectively. The recovery value was in good agreement with that determined using a manual procedure, while the standard deviation was 33% less than that found when using the manual procedure. The results from this study indicate that the continuous flow automated procedure for the determination of riboflavin in milk is a simple, quantitative method which eliminates many of the time-consuming analytical steps.

Determination of Vitamin Bi in Pharmaceutical Preparations Using a Lead Ion Selective Electrode and Atomic Absorption Spectroscopy

1979 ◽  
Vol 62 (2) ◽  
pp. 315-319
Author(s):  
Saad S M Hassan ◽  
Mohamed T Zaki ◽  
Mohamed H Eldesouki
Keyword(s):  
Standard Deviation ◽  
Atomic Absorption ◽  
Absorption Spectroscopy ◽  
Atomic Absorption Spectroscopy ◽  
Pharmaceutical Preparations ◽  
Ion Selective Electrode ◽  
Lead Ion ◽  
Fluorometric Method ◽  
Selective Electrode

Abstract Simple, rapid, and accurate methods have been developed for determining vitamin Bx in pharmaceutical preparations without prior separation of the vitamin. The methods are based on the desulfurization reaction with potassium plumbite and measurement of the unreacted lead (II) ions at 217 nm using atomic absorption spectroscopy (AAS), or by titration with EDTA at pH 4.5 with the lead ion selective electrode and Gran's plot. The results compare favorably with those obtained using the USP fluorometric method. Recoveries of pure vitamin Bx averaged 99.1%, with a standard deviation of 0.8%, and 99.0%, with a standard deviation of 0.7%, for the AAS and ion selective electrode procedures, respectively.

Fluorometric Determination of Selenium in Foods

1974 ◽  
Vol 57 (2) ◽  
pp. 368-372 ◽  
Author(s):  
Milan Ihnat
Keyword(s):  
Standard Deviation ◽  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Food Samples ◽  
Fluorometric Method ◽  
Fluorometric Determination ◽  
Relative Standard ◽  
The Mean

Abstract A fluorometric method using 2,3-diaminonaphthalene for estimating selenium has been evaluated with regard to its applicability to food samples. Charring of the sample during digestion appeared to result in losses of native and added selenium from some samples, so a modified wet digestion procedure was introduced. Digestion first in nitric acid followed by a mixture of nitric-perchloric-sulfuric acids substantially reduced the incidence of sample charring for a variety of foods. The mean apparent recovery of selenium added as selenite or selenate at 100 and 500 ng levels to 0.1 and 1.0 g corn cereal, skim milk powder, and meat and 0.1 g fish was 101.0%; the actual recovery of the same levels of selenium from standard solutions was 96.6%. For a variety of samples containing 5—750 ng native or added selenium, the standard deviation as 4.7 + 1.95 X 10-2W ng, where W = ng selenium in the sample taken for analysis. The relative standard deviation (RSD) as a function of selenium weight (ng) was 50% (10), 6.7% (100), 4.3% (200), 3.1% (400), 2.7% (600), and 2.5% (800). The detection limit (weight of selenium at which RSD = 50%) was 10 ng at a mean blank level of 25 ng.

SIMS Determination of MG+ and AS+ Range Profiles in Photoresist and Polyimide Implant Masks.

1989 ◽  
Vol 147 ◽  
Author(s):  
D. L. Dugger ◽  
M. B. Stern ◽  
T. M. Rubico
Keyword(s):  
Standard Deviation ◽  
Mass Spectroscopy ◽  
Range Data ◽  
Profile Data ◽  
Projected Range ◽  
Range Profile ◽  
P Type ◽  
Secondary Ion ◽  
Good Agreement

AbstractThe distribution of Mg+ (a p-type dopant for GaAs) and As+ (an p-type dopant for Si) implanted into both photoresist (PR) and polyimide (PI) have been determined experimentally. Range data of Mg ions at 200 keV and 300 keV and As ions at 150 keV have been measured by Secondary Ion Mass Spectroscopy (SIMS). SIMS values for the projected range Rp and the standard deviation ARp were compared to range profile data calculated using the Projected Range Algorithm (PRAL) of Biersack [1] as well as the standard LSS theory [2]. While the values for Rp calculated from the PRAL model generally agreed within 10% of the SIMS values, the calculations underestimated Rp for PR but were in good agreement for PI. The LSS calculations underestimated Rp in both materials.

Automated Fluorometric Method for Determination of Serum Calcium

1965 ◽  
Vol 11 (2) ◽  
pp. 122-130 ◽  
Author(s):  
John B Hill
Keyword(s):  
Serum Calcium ◽  
Fluorometric Method ◽  
Reproducible Method

Abstract A simple, rapid, accurate, and reproducible method for serum calcium has been developed utilizing a fully automated fluorometric system and fluorescein-complexone as the fluorophore. The results obtained agree with those obtained with the Clark-Collip permanganate titration.

Amino Acid Analysis in Feeds and Feedstuifs Using Precolumn Phenylisothiocyanate Derivatization and Liquid Chromatography—Preliminary Study

1987 ◽  
Vol 70 (3) ◽  
pp. 425-428 ◽  
Author(s):  
Rodney W Beaver ◽  
David M Wilson ◽  
Helen M Jones ◽  
Keith D Haydon
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Standard Deviation ◽  
Poultry Feed ◽  
Reverse Phase ◽  
Relative Standard ◽  
Preliminary Study ◽  
Feed Samples ◽  
Good Agreement

Abstract A method for the determination of amino acids in feed hydrolysates is described. The method uses precolumn phenylisothiocyanate (PITC) derealization and liquid chromatography on reverse phase columns with UV detection at 254 nm. The precision of the method on replicate samples of a wheat hydrolysate ranges from 1.9 to 6.7% relative standard deviation for the amino acids examined. (Met and Cys were not determined.) Recoveries were near 100% for most amino acids. Comparisons of the values obtained by PITC method with accepted wines show good agreement in wheat and poultry feed samples.

Determination of Neostigmine Bromide by Ion-Pairing Column Chromatography

1973 ◽  
Vol 56 (1) ◽  
pp. 128-131
Author(s):  
Rita E Kling
Keyword(s):  
Standard Deviation ◽  
Hydrolysis Product ◽  
Ion Pairing ◽  
Commercial Products ◽  
Product Standard ◽  
Ophthalmic Solutions ◽  
Column Chromatographic ◽  
Sensitive Method ◽  
Good Agreement

Abstract A rapid and sensitive method for the assay of neostigmine bromide (NBr) in tablets and ophthalmic solutions has been developed. NBr is isolated from its pharmaceutical excipients by the ion-pairing partition column chromatographic principles of Levine and Doyle followed by ultraviolet determination of its alkaline hydrolysis product. Standard recoveries of NBr from simulated tablet mixtures and ophthalmic solutions averaged 99.8%. The standard deviation of repeated analyses of commercial products was less than±1%. The results of the proposed method for commercial products are in good agreement with the USP XVIII procedure. The procedure is applicable to individual tablet assay.

scholarly journals Continuous Flow Techniques for the Determination of Serum Calcium and the Simultaneous Determination of Calcium and Inorganic Phosphate in Serum and Urine

1974 ◽  
Vol 11 (1-6) ◽  
pp. 81-85
Author(s):  
W. G. Brydon
Keyword(s):  
Simultaneous Determination ◽  
Serum Calcium ◽  
Continuous Flow ◽  
Inorganic Phosphate ◽  
Methyl Green ◽  
Fluorometric Method ◽  
Precision Data ◽  
Accuracy And Precision ◽  
Serum And Urine

The fluorometric method of Fingerhut et al. (1969) for the determination of serum calcium has been modified to approximately treble the sensitivity. Using only 120 μl of sample a method has been developed for the simultaneous determination of calcium and inorganic phosphate in serum and urine, the phosphate being measured by complexing phosphomolybdate with methyl green, a method developed by Van Belle (1970). Only a single dialysis stage is required, and reagents and sample can be delivered using an AutoAnalyzer Pump I. The techniques correlate well with other methods. Accuracy and precision data are presented.

Kinetic Spectrophotometric Determination of Selenium (IV) by its Catalytic Effect on the Oxidation of Acid Chrome Blue K by Hydrogen Peroxide

2012 ◽  
Vol 538-541 ◽  
pp. 2358-2363 ◽  
Author(s):  
Zhi Rong Zhou ◽  
Li Zhen Zhang
Keyword(s):  
Hydrogen Peroxide ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Limit Of Detection ◽  
Fixed Time ◽  
Relative Standard ◽  
Kinetic Spectrophotometric ◽  
Acid Chrome Blue K ◽  
Good Agreement

Based on the oxidation of acid chrome blue K (ACBK) by hydrogen peroxide in 0.002 mol/L sulfuric acid solution, while 1,10-phenanthroline (phen) acts as an activator, a simple kinetic spectrophotometric method was developed for the determination of trace amounts of Se(IV).The reaction was monitored spectrophotometrically by measuring the decrease in the absorbance of ACBK at 524 nm with a fixed-time method. The decrease in the absorbance of ACBK is proportional to the concentration of Se (IV) in the range 0.06–1.0 µg/L with a fixed time of 4–10 min from the initiation of the reaction. The limit of detection is 0.018 µg/L Se (IV). The influence of the factors such as acidity, concentration of reactants, reaction time, temperature and co-existing ions on the reaction is discussed. The optimum conditions of reaction are established and some kinetic parameters are determined. The apparent activation energy of catalytic reaction is 62.30 kJ/mol. The relative standard deviation for 11 replicate determination of 0.01 and 0.02 µg/25mL selenium (III) was calculated to be 2.3 % and 2.0 %, respectively. Combined with sulphydryl dextrane gel (SDG) separation and enriching, the method has been successfully applied to the determination of Se (IV) in foodstuff samples with the relative standard deviation of 1.1 %–3.7 % and the recovery of 99.0 %–104.0 %, the results are in good agreement with those provided by HG-AAS method.

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