Automated Method for Determination of Serum Calcium by Use of Alizarin

1970 ◽  
Vol 16 (10) ◽  
pp. 816-819 ◽  
Author(s):  
Christopher S Frings ◽  
Patricia S Cohen ◽  
Lowell B Foster
Keyword(s):  
Atomic Absorption ◽  
Serum Calcium ◽  
Coefficient Of Variation ◽  
Atomic Absorption Spectrophotometry ◽  
Manual Method ◽  
Absorption Spectrophotometry ◽  
Automated Method ◽  
Beer's Law ◽  
Beer’S Law

Abstract An automated method is described for determining serum calcium by measuring the red chromogenic complex formed by calcium and alizarin. About 40 µl of undialyzed serum is added directly to one reagent to pro-duce the color, which follows Beer's law at 599 nm to a concentration of 15.0 mg of calcium per 100 ml, with a coefficient of variation of 2.5%. Serum calcium concentrations, as measured by our method, compare favorably with those by the corresponding manual method and by atomic absorption spectrophotometry

Determination of Soy Products in Meat-Soy Blends

1974 ◽  
Vol 57 (4) ◽  
pp. 841-846
Author(s):  
Marvin W Formo ◽  
Guy R Honold ◽  
David B Maclean
Keyword(s):  
Standard Deviation ◽  
Atomic Absorption ◽  
Computer Analysis ◽  
Coefficient Of Variation ◽  
Ground Beef ◽  
Atomic Absorption Spectrophotometry ◽  
Average Concentration ◽  
Soy Flour ◽  
Absorption Spectrophotometry

Abstract The concentrations of various elements and fiber in ground beef and textured soy flour were measured, respectively, by atomic absorption spectrophotometry and a modified AOAC fiber method. The average concentration, standard deviation, and coefficient of variation for magnesium, manganese, and fiber, respectively, in textured soy flour are 2948 mg/kg, 186 mg/kg, 6.3% ; 33.4 mg/kg, 3.4 mg/kg, 10.1% ; 2.03%, 0.27%, 13.3%. The average concentration, standard deviation, and coefficient of variation for magnesium, manganese, and fiber, respectively, in regular ground beef are 151 mg/kg, 7.4 mg/kg, 4.9% ; 0.125 mg/kg, 0.025 mg/kg, 20%; 0.01%. From computer analysis of the data from an interlaboratory study of the analysis of textured soy flour–ground beef mixtures, the expected total errors (one standard deviation in absolute percentages) of the method applied to a product containing 20% textured soy flour hvdrated 1+2 were as follows for the respective indices used: magnesium 1.8%, manganese 2.7%, and fiber 8.8%. Analysis of ground beef and textured soy flour for ash, hemicellulose, stachyose, phosphorus, potassium, copper, and zinc revealed that these constituents were not suitable indices of textured soy flour content in textured soy flour-ground beef mixtures.

Application of Atomic Absorption Spectrophotometry in the Determination of Calcium in Serum

1964 ◽  
Vol 10 (10) ◽  
pp. 869-890 ◽  
Author(s):  
Alfred Zettner ◽  
David Seligson
Keyword(s):  
Calcium Oxalate ◽  
Atomic Absorption ◽  
Serum Calcium ◽  
Atomic Absorption Spectrophotometry ◽  
Dilution Technique ◽  
Serum Samples ◽  
Absorption Spectrophotometry

Abstract Atomic absorption spectrophotometry (AAS) was applied to the determination of serum calcium. A special diluent was developed to abolish the effect of absorption depressors. This made possible measurement of calcium in directly diluted serum samples without prior removal of any serum constituents. Values obtained by AAS on calcium oxalate precipitates from serum were identical with those found by the direct dilution technique.

Determination of Serum Calcium by Automated Atomic Absorption Spectroscopy

1967 ◽  
Vol 13 (5) ◽  
pp. 388-396 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman ◽  
Stanley Morgenstern
Keyword(s):  
Hydrochloric Acid ◽  
Atomic Absorption ◽  
Serum Calcium ◽  
Flow System ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Comparison Of The Results ◽  
Automated Determination ◽  
Good Agreement

Abstract A procedure is presented for the automated determination of serum calcium by atomic absorption spectrophotometry. The serum sample, diluted with acidified lanthanum dichloride, is dialyzed against 0.1 N hydrochloric acid and a portion of the recipient solution is pumped into the atomizer-burner of the spectrophotometer. Data are presented on the precision and reproducibility of the flow system. Addition of calcium to serum shows a mean recovery of 99%. A comparison of the results of analyses on random specimens by permanganate titration and the automated procedure shows good agreement.

Automated Simultaneous Determination of p-Aminohippurate and Creatinine in Plasma or Urine

1974 ◽  
Vol 20 (3) ◽  
pp. 348-352 ◽  
Author(s):  
C K Parekh ◽  
J Kirpan ◽  
A Peterson ◽  
G L Hassert ◽  
B F Murphy
Keyword(s):  
Simultaneous Determination ◽  
Urine Samples ◽  
Manual Method ◽  
Automated Method ◽  
Discrete Sample ◽  
Beer's Law ◽  
Plasma And Urine Samples ◽  
Beer’S Law

Abstract Methods have been developed for using the Beckman "Discrete Sample Analyzer" (DSA-560), for automated simultaneous determinations of p-aminohippurate (PAH) and creatinine in the same 50- or 10-µl samples of plasma or urine, respectively, at the rate of 80 samples per hour. In determinations of PAH, a single reagent, p-dimethylaminocinnamaldehyde in 0.1 mol/liter HCl, was added to a protein-free filtrate from plasma or urine. The intensity of the color, measured at 550 nm, obeyed Beer’s law for PAH concentrations from 0.005 to 5.0 g/liter. Data obtained for the same plasma and urine samples by the manual method, were, in general, within ±5% of the results obtained by the automated method. Creatinine was simultaneously determined by adapting the method of Taussky to the DSA-560 instrument; the intensity of the color, measured at 510 nm, obeyed Beer's law for creatinine concentrations ranging from 0.005 to 5.0 g/liter. Data obtained for the same plasma and urine samples by the manual method, were, in general, within ± 5% of the results obtained by the automated method.

An Automated Micromethod for Determination of Serum Glucose, with an Improved o-Toluidine Reagent

1970 ◽  
Vol 16 (4) ◽  
pp. 285-290 ◽  
Author(s):  
Wells R Moorehead ◽  
Edward A Sasse
Keyword(s):  
Life Expectancy ◽  
Polyvinyl Chloride ◽  
Coefficient Of Variation ◽  
Serum Glucose ◽  
Automated Method ◽  
Beer's Law ◽  
Beer’S Law ◽  
Manual Methods ◽  
Reducing Properties

Abstract An automated method has been developed in which a new o-toluidine reagent is used for the microanalysis of glucose. The reagent is 1.3 times as sensitive as the o-toluidine reagent commonly used in manual procedures. The reagent does not significantly alter the life expectancy of manifold tubing made of polyvinyl chloride. The automation includes a dialysis step and possesses the desirable features of the manual methods, including the greater specificity as compared with methods based on reducing properties. Results of this automated method obey Beer’s law, are very reproducible (the coefficient of variation is approximately 1.5%), and are not significantly different from those obtained manually. The method requires 75 µl of sample.

Automated Atomic Absorption Spectrophotometry

1967 ◽  
Vol 13 (9) ◽  
pp. 788-796 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman ◽  
Morris Oklander
Keyword(s):  
Atomic Absorption ◽  
Excellent Agreement ◽  
Serum Calcium ◽  
Flow System ◽  
Serum Magnesium ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Present Procedure ◽  
Comparison Of The Results

Abstract The flow system developed for the determination of serum calcium by automated atomic absorption spectrophotometry (AAS) was adapted to the determination of serum magnesium. A comparison of the results of analyses by the present procedure and by an automated fluorometric procedure on identical serum specimens shows excellent agreement.

Determination of Cadmium in Blood and Urine by Graphite Furnace Atomic Absorption Spectrophotometry

1975 ◽  
Vol 21 (4) ◽  
pp. 626-629 ◽  
Author(s):  
Elizabeth F Perry ◽  
S Roy Koirtyohann ◽  
H Mitchell Perry
Keyword(s):  
Detection Limit ◽  
Blood Plasma ◽  
Atomic Absorption ◽  
Coefficient Of Variation ◽  
Graphite Furnace ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Graphite Furnace Atomic Absorption ◽  
Wet Ashing

Abstract We describe a method for measuring quantities of cadmium in blood, plasma, and urine by using graphite furnace atomic absorption preceded by wet ashing. The method appears to be extremely accurate and reproducible (coefficient of variation is 7% for 2.8 µg/liter concentration), with a detection limit of 2 pg.

Automated Atomic Absorption Spectrophotometry

1967 ◽  
Vol 13 (12) ◽  
pp. 1079-1087 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman
Keyword(s):  
Atomic Absorption ◽  
Simultaneous Determination ◽  
Serum Calcium ◽  
Flow System ◽  
Atomic Absorption Spectrophotometry ◽  
Reducing Agent ◽  
Molybdenum Blue ◽  
Absorption Spectrophotometry ◽  
Blue Reaction

Abstract A procedure is described for the automated simultaneous determination of serum calcium and phosphate. Calcium is assayed by atomic absorption spectrophotometry. Phosphate is determined by the molybdenum blue reaction in an improved flow system with a more sensitive reducing agent.

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