Screening for Albuminuria: A Case for Estimation of Albumin in Urine

1975 ◽  
Vol 21 (12) ◽  
pp. 1795-1798 ◽  
Author(s):  
E BenGershôm
Keyword(s):  
Total Protein ◽  
Urinary Albumin ◽  
Green Method ◽  
Green Is ◽  
Protein Estimation ◽  
Microzone Electrophoresis ◽  
Bromcresol Green

Abstract The usefulness of bromcresol green for estimating albumin in urine was evaluated by comparison with the Laurell "rocket" technique. In contrast to the bromcresol green method applied for urinary albumin, rather doubtful results were obtained with conventional (Microzone) electrophoresis for albumin and with precipitation techniques for total protein estimation. Albumin estimation with bromcresol green is recommended as a more reliable substitute for total-protein estimations in urine. Limitations of bromcresol green are also pointed out.

Urinary albumin determination by the immediate bromcresol green method

1978 ◽  
Vol 90 (3) ◽  
pp. 241-248 ◽  
Author(s):  
Jan B.C. Gustafsson ◽  
H.René Uzqeda B.
Keyword(s):  
Urinary Albumin ◽  
Green Method ◽  
Bromcresol Green

Comparison of Results from the Du Pont "ACA" and Technicon "SMA 12/ 60"

1972 ◽  
Vol 18 (4) ◽  
pp. 340-348 ◽  
Author(s):  
James O Westgard ◽  
Brenda L Lahmeyer
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Normal Range ◽  
Green Method ◽  
Comparison Of Results ◽  
Bromcresol Green ◽  
Better Than

Abstract The Du Pont "Automatic Clinical Analyzer" (ACA) and the Technicon "SMA 12/60" were compared as to precision, range of linearity, and results for sera from patients. Precision of the ACA was equal to or better than that of the SMA 12/60 for all determinations except SGOT in the normal range. The ACA showed a wider range of linearity for BUN, total protein, LDH, alkaline phosphatase, SGOT, and bilirubin, and equal linear ranges for glucose and albumin. Patient’s sera comparisons showed differences for glucose in uremic sera. Albumin values from the ACA did not compare well with those by HABA dye method from the SMA 12/60, but did compare well with values obtained by electrophoresis and by a bromcresol green method adapted to the SMA 12/60. Bilirubin values from the ACA were higher than those from the SMA 12/60. Comparable results were obtained for the enzyme determinations when the SMA 12/60 was calibrated in the same units as the ACA.

Comparison of several methods for semiquantitative determination of urinary protein.

1977 ◽  
Vol 23 (5) ◽  
pp. 876-879 ◽  
Author(s):  
W L Gyure
Keyword(s):  
Serum Albumin ◽  
Salt Concentration ◽  
Urine Samples ◽  
Urine Protein ◽  
Sulfosalicylic Acid ◽  
Green Method ◽  
Semiquantitative Determination ◽  
Acid Method ◽  
Bromcresol Green

Abstract Two types of urine protein dipsticks and the sulfosalicylic acid method were compared for their accuracy and specificity, with use of urine samples supplemented with various proteins. Dipsticks yield accurate results when the protein under consideration is restricted to albumin; the sulfosalicylic acid method accurately determines many kinds of proteins in addition to albumin. Detergents affect each of the methods, but changes in salt concentration only affect results by dipstick procedures. Dipsticks, which are based on the protein-error principle for indicators, are subject to some of the conditions that apply to the bromcresol green method for serum albumin determination.

Effects of serum-isolated vs synthetic bilirubin-albumin complexes on dye-binding methods for estimating serum albumin

1991 ◽  
Vol 37 (7) ◽  
pp. 1269-1272 ◽  
Author(s):  
H Ihara ◽  
H Nakamura ◽  
Y Aoki ◽  
T Aoki ◽  
M Yoshida
Keyword(s):  
Serum Albumin ◽  
Molar Ratio ◽  
Green Method ◽  
Dye Binding ◽  
Bromcresol Green

Abstract Bush and Reed reported (Clin Chem 1987;33:821-3) that the reaction of albumin with bromcresol purple but not with bromcresol green underestimated the concentration of albumin in synthetically obtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indistinguishable from the natural Bd in icteric serum. Here we verify that Bd, whether synthetically obtained or isolated from serum, causes an underestimation of albumin in the bromcresol purple but not in the bromcresol green method. The molar ratio of Bd from either source to underestimated albumin approximates 1.0, suggesting that one molecule of Bd would react equivalently to a molecule of albumin in the bromcresol purple method. This underestimation might falsely suggest hypoalbuminemia in patients with increased serum Bd.

Normal values of urinary albumin and total protein excretion during pregnancy

1994 ◽  
Vol 171 (4) ◽  
pp. 984-989 ◽  
Author(s):  
Kenneth Higby ◽  
Cheryl R. Suiter ◽  
John Y. Phelps ◽  
Theresa Siler-Khodr ◽  
Oded Langer
Keyword(s):  
Total Protein ◽  
Normal Values ◽  
Urinary Albumin ◽  
Protein Excretion

Improved continuous-flow (SMAC) determination of serum albumin.

1978 ◽  
Vol 24 (7) ◽  
pp. 1191-1193 ◽  
Author(s):  
G Cederblad ◽  
B E Hickey ◽  
A Hollender ◽  
G Akerlund
Keyword(s):  
Reaction Time ◽  
Serum Albumin ◽  
Acute Phase ◽  
Continuous Flow ◽  
Green Method ◽  
Acute Phase Reactants ◽  
Modified Method ◽  
Continuous Flow Method ◽  
Bromcresol Green

Abstract The albumin values determined by the bromcresol green methods do not compare well with values by more specific methods for albumin determination. The discrepancies have been related to, among other things, acute-phase reactants and are especially pronounced in the lower albumin range. These disadvantages are also inherent in a routine continuous-flow method for albumin (SMAC). The bromcresol green method has been improved considerably by shortening the reaction time before the absorbance is measured, as is described here. The modified method yields values that better agree with those by more specific methods and an influence of acute-phase reactants is no longer observed.

Some Methodological Modifications of the Technicon "SMA 12/60 AutoAnalyzer" System

1972 ◽  
Vol 18 (7) ◽  
pp. 630-642 ◽  
Author(s):  
Tevfik K Bigat ◽  
Abraham Saifer
Keyword(s):  
Alkaline Phosphatase ◽  
Glucose Oxidase ◽  
Aspartate Aminotransferase ◽  
Inorganic Phosphate ◽  
New Method ◽  
Green Method ◽  
Bromcresol Green ◽  
Made In

Abstract Methodological modifications have been made in our "SMA 12/60 AutoAnalyzer" system, to improve the reproducibility and accuracy of six commonly performed procedures. These include glucose with the glucose oxidase method, albumin with the bromcresol green method, and alkaline phosphatase with the sodium thymolphthalein monophosphate method. We have also modified the composition of the reagents used in the determination of protein, inorganic phosphate, and aspartate aminotransferase. A general approach that is useful for adapting a new method to a multichannel AutoAnalyzer system is discussed.

Comparison of Urinary Albumin and Urinary Total Protein as Predictors of Patient Outcomes in CKD

2011 ◽  
Vol 57 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Shona Methven ◽  
Mark S. MacGregor ◽  
Jamie P. Traynor ◽  
Mario Hair ◽  
Denis St J. O'Reilly ◽  
...  
Keyword(s):  
Patient Outcomes ◽  
Total Protein ◽  
Urinary Albumin
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