Creatine kinase isoenzyme patterns in human tissue obtained at surgery.

1976 ◽  
Vol 22 (2) ◽  
pp. 173-175 ◽  
Author(s):  
S H Tsung
Keyword(s):  
Creatine Kinase ◽  
Human Tissue ◽  
Creatine Kinase Isoenzyme ◽  
Quantitative Distribution ◽  
Immunological Method ◽  
Creatine Kinase Isoenzymes ◽  
Isoenzyme Patterns ◽  
Postmortem Autolysis

Abstract Chromatography on DEAE-Sephadex A-50 was applied to study the quantitative distribution of creatine kinase isoenzymes in extracts of human tissue obtained during surgery. The results are compared with those determined by an immunological method [Clin. Chim. Acta 58, 223 (1975)]. Conflicting results for some organs as reported by the two methods are probably attributable to postmortem autolysis.

Effect of serum pH on storage stability and reaction lag phase of human creatine kinase isoenzymes.

1980 ◽  
Vol 26 (8) ◽  
pp. 1165-1169 ◽  
Author(s):  
D A Nealon ◽  
S M Pettit ◽  
A R Henderson
Keyword(s):  
Creatine Kinase ◽  
Ethylene Glycol ◽  
Storage Stability ◽  
Kinase Assay ◽  
Lag Phase ◽  
Creatine Kinase Isoenzyme ◽  
Sh Groups ◽  
C Storage ◽  
Creatine Kinase Isoenzymes

Abstract We report the effect of serum pH on the storage stability of the human creatine kinase isoenzymes and on the creatine kinase assay lag phase (Szasz et al., Clin. Chem. 22: 650, 1976). We also investigated the effect of including mercaptoethanol, N-acetyl-L-cysteine, monothioglycerol, ethylenediaminetetraacetate, or ethylene glycol bis(betaaminoethyl ether)-N,N,N',N'-tetraacetate at 20, 4, and --20 degrees C. Storage stability of the isoenzymes is profoundly affected by pH. For patients' samples and semi-purified human creatine kinase isoenzymes added to heat-inactivated sera, increasing diluent pH above 7.0 decreases creatine kinase stability. The thiol agents or chelators generally give little or no protection above pH 7.5; at pH 8.5 they contribute significantly to isoenzyme instability. Storage at 4 degrees C provides greater stability than storage at 20 degrees C, particularly in the case of creatine kinase isoenzyme BB. The lag phase was minimum at a serum pH of 6.5, in the presence of 10 mmol of monothioglycerol per liter. Increasing serum pH to 8.5 prolongs the reaction lag phase by about 1 min over the minimum. We recommend that, before they are stored at 4 degrees C, the pH of patients' samples be adjusted to 6.5 and oxidation of SH-groups be minimized by adding monothioglycerol to the sample.

Isoenzymes of creatine kinase in extracts of various parts and regions of the human central nervous system.

1980 ◽  
Vol 26 (6) ◽  
pp. 760-762 ◽  
Author(s):  
R R Petronia ◽  
A H Maas ◽  
C W van Veelen ◽  
G E Staal
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Creatine Kinase ◽  
Gel Electrophoresis ◽  
Cauda Equina ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Human Central Nervous System ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Isoenzymes

Abstract The distribution of isoenzymes of creatine kinase (EC 2.7.3.2.) was investigated by agarose gel electrophoresis of extracts of selected parts and regions of the human central nervous system. Besides the major brain isoenzyme BB, we demonstrated the presence of three other creatine kinase isoenzyme forms. The distribution of creatine kinase isoenzymes depending strongly on the region from which the biopsy was taken. We found substantial amounts of the MB isoenzyme in extracts of the dura from the cauda equina of the two adults examined.

Creatine kinase* isoenzyme patterns in Duchenne muscular dystrophy

2008 ◽  
Vol 14 (5) ◽  
pp. 257-260 ◽  
Author(s):  
H. W. Goedde ◽  
I. Christ ◽  
H. G. Benkmann ◽  
R. Beckmann ◽  
H. Lang
Keyword(s):  
Creatine Kinase ◽  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Creatine Kinase Isoenzyme ◽  
Isoenzyme Patterns

Creatine kinase isoenzyme patterns in neoplasms of peripheral nerve.

1980 ◽  
Vol 26 (13) ◽  
pp. 1888-1889 ◽  
Author(s):  
J Balaton ◽  
G N Hoag ◽  
C R Franks ◽  
C R Smith ◽  
W E DeCoteau
Keyword(s):  
Creatine Kinase ◽  
Peripheral Nerve ◽  
Column Chromatography ◽  
Trace Amount ◽  
Isoenzyme Pattern ◽  
Malignant Schwannoma ◽  
Creatine Kinase Isoenzyme ◽  
Neoplastic Lesions ◽  
Isoenzyme Patterns

Abstract Normal peripheral nerve and neoplastic lesions of peripheral nerve varied in their creatine kinase (CK; EC 2.7.3.2) isoenzyme pattern, as assessed both with electrophoresis and with column chromatography. All three isoenzymes were seen in normal peripheral nerve, but the peripheral nerve tumors, neurofibroma and neurilemmoma, demonstrated predominantly CK-1 isoenzyme activity, with a trace amount of CK-3. No CK-2 activity was demonstrated in these tumors. In contrast, malignant schwannoma tissue contained all three isoenzymes, but in a different proportion than in normal peripheral nerve.

Creatine kinase isoenzyme patterns in normal smooth muscle and smooth muscle neoplasms

1980 ◽  
Vol 13 (4) ◽  
pp. 149-150 ◽  
Author(s):  
G.N. Hoag ◽  
C.R. Franks ◽  
C. Smith ◽  
W.E. DeCoteau
Keyword(s):  
Creatine Kinase ◽  
Smooth Muscle ◽  
Creatine Kinase Isoenzyme ◽  
Isoenzyme Patterns

Human placenta as a convenient source of creatine kinase BB.

1983 ◽  
Vol 29 (8) ◽  
pp. 1537-1539 ◽  
Author(s):  
J P Steghens ◽  
I Maire ◽  
M Mathieu
Keyword(s):  
Creatine Kinase ◽  
Human Placenta ◽  
Catalytic Properties ◽  
Adenosine Diphosphate ◽  
Creatine Phosphate ◽  
Abdominal Muscle ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Bb ◽  
Creatine Kinase Isoenzymes

Abstract We demonstrate that human placenta is a convenient source of creatine kinase isoenzyme BB. We compare the physicochemical and catalytic properties with those of other creatine kinase isoenzymes: purified human abdominal muscle MM and brain BB. We also describe a stabilizing medium for creatine kinase BB. Human placental and brain BB have similar catalytic properties, the respective Km values for creatine phosphate being 0.66 and 0.56 mmol/L and for adenosine diphosphate 89 and 70 nmol/L.

Isoenzymes of creatine kinase in extracts of various parts and regions of the human central nervous system.

1980 ◽  
Vol 26 (6) ◽  
pp. 760-762 ◽  
Author(s):  
R R Petronia ◽  
A H Maas ◽  
C W van Veelen ◽  
G E Staal
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Creatine Kinase ◽  
Gel Electrophoresis ◽  
Cauda Equina ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Human Central Nervous System ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Isoenzymes

Abstract The distribution of isoenzymes of creatine kinase (EC 2.7.3.2.) was investigated by agarose gel electrophoresis of extracts of selected parts and regions of the human central nervous system. Besides the major brain isoenzyme BB, we demonstrated the presence of three other creatine kinase isoenzyme forms. The distribution of creatine kinase isoenzymes depending strongly on the region from which the biopsy was taken. We found substantial amounts of the MB isoenzyme in extracts of the dura from the cauda equina of the two adults examined.

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