Interference of imferon in colorimetric assays for iron.

1980 ◽  
Vol 26 (5) ◽  
pp. 635-637 ◽  
Author(s):  
W Huisman
Keyword(s):  
Ascorbic Acid ◽  
Ferrous Sulfate ◽  
Iron Complex ◽  
Iron Dextran ◽  
Ammonium Ferrous Sulfate ◽  
Homogeneous Assay ◽  
Colorimetric Assays ◽  
Iron Interference ◽  
Minimum Interference ◽  
Assay Conditions

Abstract Imferon (Merrell-National), an iron-dextran complex, is widely used in patients with iron deficiency. It is present in the circulation in appreciable amounts for two to three weeks after administration and interferes with all tested colorimetric iron assays, both with and without deproteinization. The amount of the plasma Imferon iron interference depends primarily on the choice of reductant. With dithionite it is essentially 100%. In the presence of ascorbic acid and hydroxylamine, the interference depends also on assay conditions, especially temperature, but also incubation time and pH. The minimum interference in a homogeneous assay was about 3%. The relative amount of interference from hemoglobin iron under the various assay conditions is different from that of Imferon iron. In the presence of a reducing agent, the dextran-iron complex decomposes--instantaneously with dithionite, and gradually with sulfite, ascorbic acid, and hydroxylamine. The freed iron becomes dialyzable, can react with bathophenanthroline, and elutes on a Sephadex G-50 or G-15 column in the same fractions as an ammonium ferrous sulfate.

Interference of imferon in colorimetric assays for iron.

1980 ◽  
Vol 26 (5) ◽  
pp. 635-637
Author(s):  
W Huisman
Keyword(s):  
Ascorbic Acid ◽  
Ferrous Sulfate ◽  
Iron Complex ◽  
Iron Dextran ◽  
Ammonium Ferrous Sulfate ◽  
Homogeneous Assay ◽  
Colorimetric Assays ◽  
Iron Interference ◽  
Minimum Interference ◽  
Assay Conditions

Abstract Imferon (Merrell-National), an iron-dextran complex, is widely used in patients with iron deficiency. It is present in the circulation in appreciable amounts for two to three weeks after administration and interferes with all tested colorimetric iron assays, both with and without deproteinization. The amount of the plasma Imferon iron interference depends primarily on the choice of reductant. With dithionite it is essentially 100%. In the presence of ascorbic acid and hydroxylamine, the interference depends also on assay conditions, especially temperature, but also incubation time and pH. The minimum interference in a homogeneous assay was about 3%. The relative amount of interference from hemoglobin iron under the various assay conditions is different from that of Imferon iron. In the presence of a reducing agent, the dextran-iron complex decomposes--instantaneously with dithionite, and gradually with sulfite, ascorbic acid, and hydroxylamine. The freed iron becomes dialyzable, can react with bathophenanthroline, and elutes on a Sephadex G-50 or G-15 column in the same fractions as an ammonium ferrous sulfate.

scholarly journals Quantitative Assessment of the Effects of Reducing Agents on Biological Macromolecules and on the Possible Repair of Oxidative Damage

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Jianan Zhao ◽  
Naijin Xu ◽  
Hui Liu
Keyword(s):  
Ascorbic Acid ◽  
Oxidative Damage ◽  
Potassium Iodide ◽  
Gel Electrophoresis ◽  
Ferrous Sulfate ◽  
Reducing Agents ◽  
Significant Inhibition ◽  
Enzyme Linked Immunosorbent Assay ◽  
Biological Macromolecules ◽  
Ammonium Ferrous Sulfate

Objective. To quantitatively assess the influence of reducing agents on biological macromolecules and on the possible repair of oxidative damage. Methods. Samples (antibody, enzyme, DNA, and diluted serum) were treated with reducing agents (ammonium ferrous sulfate, ascorbic acid, potassium iodide, and sodium hyposulfite) in the experimental group and with NaCl in the control group. Enzyme-linked immunosorbent assay and quantitative PCR were used to determine the activity of antibody, enzyme, and DNA. Native gel electrophoresis (Native-PAGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were used to determine protein structure. Reducing agents that had no inhibitory effect on biological macromolecules were selected. Antibodies were treated with oxidants to caused oxidative damage and then treated with reducing agents, and the possible repair of oxidative damage was assessed. Results. Certain concentrations of ammonium ferrous sulfate resulted in significant inhibition of antibody, enzyme, DNA, and diluted serum. Certain concentrations of ascorbic acid resulted in significant inhibition of antibody. Sodium hyposulfite and potassium iodide had no effect on antibody, enzyme, DNA, and diluted serum. The OD values in group A (in which HBsAb was treated by oxidation and then a reductant) were significantly higher than those in group B (HBsAb treated by oxidation). Conclusion. Ammonium ferrous sulfate, ascorbic acid, sodium hyposulfite, and potassium iodide had different effects on antibody, enzyme, DNA, and diluted serum. The reduction in antibody activity due to an oxidant was partially repaired by a reductant.

Hepcidin inhibition improves iron homeostasis in ferrous sulfate and LPS treatment model in mice

Drug Research ◽  
2021 ◽  
Author(s):  
Vishal Patel ◽  
Amit Joharapurkar ◽  
Samadhan Kshirsagar ◽  
Maulik Patel ◽  
Hiren Patel ◽  
...  
Keyword(s):  
Ferrous Sulfate ◽  
Serum Iron ◽  
Iron Homeostasis ◽  
The Body ◽  
Rapid Screening ◽  
Iron Dextran ◽  
Liver Iron ◽  
Hepcidin Expression ◽  
Serum Hepcidin ◽  
Lps Treatment

Abstract Background Hepcidin, a liver-derived peptide, regulates the absorption, distribution, and circulation of iron in the body. Inflammation or iron overload stimulates hepcidin release, which causes the accumulation of iron in tissues. The inadequate levels of iron in circulation impair erythropoiesis. Inhibition of hepcidin may increase iron in circulation and improve efficient erythropoiesis. Activin-like kinase (ALK) inhibitors decrease hepcidin. Methods In this work, we have investigated an ALK inhibitor LDN193189 for its efficacy in iron homeostasis. The effect of LDN193189 treatment was assessed in C57BL6/J mice, in which hepcidin was induced by either ferrous sulfate or lipopolysaccharide (LPS) injection. Results After two hours of treatment, ferrous sulfate increased serum and liver iron, serum hepcidin, and liver hepcidin expression. On the other hand, LPS reduced serum iron in a dose-related manner after six hours of treatment. LDN193189 treatment increased serum iron, decreased spleen and liver iron, decreased serum hepcidin and liver hepcidin expression in ferrous sulfate-treated mice, and increased serum iron in LPS-induced hypoferremia. We observed that ferrous sulfate caused a significantly higher increase in liver iron, serum hepcidin, and liver hepcidin than turpentine oil or LPS in mice. Iron dextran (intraperitoneal or intravenous) increased serum iron, but LDN193189 did not show hyperferremia with iron dextran stimulus. Conclusion Ferrous sulfate-induced hyperferremia can be a valuable and rapid screening model for assessing the efficacy of hepcidin inhibitors.

scholarly journals Fortification Iron as Ferrous Sulfate Plus Ascorbic Acid Is More Rapidly Absorbed Than as Sodium Iron EDTA but Neither Increases Serum Nontransferrin-Bound Iron in Women

2011 ◽  
Vol 141 (5) ◽  
pp. 822-827 ◽  
Author(s):  
Barbara Troesch ◽  
Ines Egli ◽  
Christophe Zeder ◽  
Richard F. Hurrell ◽  
Michael B. Zimmermann
Keyword(s):  
Ascorbic Acid ◽  
Ferrous Sulfate

scholarly journals Comparative Study of Novel Ratio Spectra and Isoabsorptive Point Based Spectrophotometric Methods: Application on a Binary Mixture of Ascorbic Acid and Rutin

2016 ◽  
Vol 2016 ◽  
pp. 1-12
Author(s):  
Hany W. Darwish ◽  
Ahmed H. Bakheit ◽  
Ibrahim A. Naguib
Keyword(s):  
Ascorbic Acid ◽  
Binary Mixture ◽  
High Performance ◽  
Pharmaceutical Tablets ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
Ratio Difference ◽  
Mean Centering ◽  
Assay Conditions

This paper presents novel methods for spectrophotometric determination of ascorbic acid (AA) in presence of rutin (RU) (coformulated drug) in their combined pharmaceutical formulation. The seven methods are ratio difference (RD), isoabsorptive_RD (Iso_RD), amplitude summation (A_Sum), isoabsorptive point, first derivative of the ratio spectra (1DD), mean centering (MCN), and ratio subtraction (RS). On the other hand, RU was determined directly by measuring the absorbance at 358 nm in addition to the two novel Iso_RD and A_Sum methods. The work introduced in this paper aims to compare these different methods, showing the advantages for each and making a comparison of analysis results. The calibration curve is linear over the concentration range of 4–50 μg/mL for AA and RU. The results show the high performance of proposed methods for the analysis of the binary mixture. The optimum assay conditions were established and the proposed methods were successfully applied for the assay of the two drugs in laboratory prepared mixtures and combined pharmaceutical tablets with excellent recoveries. No interference was observed from common pharmaceutical additives.

Dyeing of Pearl Fiber by Walnut Peel Dye

2012 ◽  
Vol 441 ◽  
pp. 66-71 ◽  
Author(s):  
Chao Ping Dong ◽  
Jie Dong ◽  
Jian Ming Xia
Keyword(s):  
Ferrous Sulfate ◽  
Evaluation Index ◽  
Single Factor ◽  
Dyeing Process ◽  
Ammonium Ferrous Sulfate ◽  
Liquor Ratio ◽  
Orthogonal Experiments ◽  
Dyeing Method ◽  
Pearl Fiber ◽  
Peel Extract

This paper studied the metachrome process of an optimal dyeing method by comparing the color and fastness of walnut peel extract applied on the pearl fiber with ammonium ferrous sulfate as mordent. In addition, the paper also ascertained the reasonable dyeing process, that is, pH 9, walnut peel extract (the dye) dosage 200 ml/L, mordant (ammonium ferrous sulfate) 2.5 g/L, temperature 80 °C, time 60 min and liquor ratio 1:30, through analysis on single-factor experiments and orthogonal experiments by taking K/S as an evaluation index for the dyeing effect. The results showed that a better effect is achievable by dyeing the pearl fiber with walnut peel dye through the metachrome process.

scholarly journals Iron chlorosis on dracaena sanderiana

1969 ◽  
Vol 37 (4) ◽  
pp. 265-272
Author(s):  
George Samuels ◽  
Héctor R. Cibes
Keyword(s):  
Puerto Rico ◽  
Metallic Iron ◽  
Ferrous Sulfate ◽  
Iron Complex ◽  
Iron Chlorosis ◽  
Dracaena Sanderiana ◽  
Poorly Drained Soils

A study of a chlorosis on Dracaena sanderiana in Puerto Rico disclosed the following facts: 1. The chlorosis which consists of a yellowing of the younger rather than the older leaves of the sanderiana is caused by a lack of available iron. 2. The chlorosis was found in soils with pH's ranging from 4.2 to 7.5 and with textures from loamy sands to heavy clays. It was more prevalent on poorly drained soils. 3. The use of ferrous sulfate sprays gave unsatisfactory results because of a spotting or uneven greening of the leaf and an unsightly residue which could be removed only with difficulty by washing. 4. The use of Fe-EDTA (12 percent as metallic iron), an organic iron complex as a spray (1 pound per 100 gallons) gave satisfactory control without leaving any spray residues or causing spotting. 5. Fe-EDTA gave satisfactory results when applied to the roots in solution, but took longer to work than did the Fe-EDTA spray. 6. General recommendations are given for the control of iron chlorosis in sanderianas.

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