Method for determination of hydrogen peroxide, with its application illustrated by glucose assay.

1980 ◽  
Vol 26 (5) ◽  
pp. 658-660 ◽  
Author(s):  
E Graf ◽  
J T Penniston
Keyword(s):  
Hydrogen Peroxide ◽  
Colorimetric Method ◽  
Ammonium Molybdate ◽  
Analyte Concentration ◽  
Glucose Assay ◽  
Color Development ◽  
Iodine Complex ◽  
Biological Compounds ◽  
Illustrative Application

Abstract We describe a simple colorimetric method for determining micromolar quantities of hydrogen peroxide, based on the oxidation of iodide in the presence of ammonium molybdate and photometry of the resulting blue starch-iodine complex. Color development is linearly dependent on analyte concentration, but only slightly time dependent, and the color of the complex formed is stable for several hours. In the range of wavelengths that may be used (570 to 630 nm), lack of interference from other biological compounds makes this method seem suitable for routine analyses. As one illustrative application of the method we quantitated glucose by measuring hydrogen peroxide produced from it by glucose oxidase catalysis. This method of quantitating glucose is more than five times as sensitive as the commonly used dianisidine method. With the appropriate hydrogen peroxide-producing oxidases, this method may be used to directly measure amino acids, purines, uric acid, xanthine, and hypoxanthine.

Method for determination of hydrogen peroxide, with its application illustrated by glucose assay.

1980 ◽  
Vol 26 (5) ◽  
pp. 658-660
Author(s):  
E Graf ◽  
J T Penniston
Keyword(s):  
Hydrogen Peroxide ◽  
Colorimetric Method ◽  
Ammonium Molybdate ◽  
Analyte Concentration ◽  
Glucose Assay ◽  
Color Development ◽  
Iodine Complex ◽  
Biological Compounds ◽  
Illustrative Application

Abstract We describe a simple colorimetric method for determining micromolar quantities of hydrogen peroxide, based on the oxidation of iodide in the presence of ammonium molybdate and photometry of the resulting blue starch-iodine complex. Color development is linearly dependent on analyte concentration, but only slightly time dependent, and the color of the complex formed is stable for several hours. In the range of wavelengths that may be used (570 to 630 nm), lack of interference from other biological compounds makes this method seem suitable for routine analyses. As one illustrative application of the method we quantitated glucose by measuring hydrogen peroxide produced from it by glucose oxidase catalysis. This method of quantitating glucose is more than five times as sensitive as the commonly used dianisidine method. With the appropriate hydrogen peroxide-producing oxidases, this method may be used to directly measure amino acids, purines, uric acid, xanthine, and hypoxanthine.

scholarly journals A colorimetric method for rapid and selective quantification of peroxodisulfate, peroxomonosulfate and hydrogen peroxide

2017 ◽  
Vol 2 (4) ◽  
pp. 462-466 ◽  
Author(s):  
Benjamin J. Deadman ◽  
Klaus Hellgardt ◽  
King Kuok (Mimi) Hii
Keyword(s):  
Hydrogen Peroxide ◽  
Colorimetric Method ◽  
Selective Method

A convenient and selective method for the determination of peroxodisulfate, peroxomonosulfate and hydrogen peroxide in mixed oxidant systems.

scholarly journals The determination of silicate in sea water

1951 ◽  
Vol 30 (1) ◽  
pp. 149-160 ◽  
Author(s):  
F. A. J. Armstrong
Keyword(s):  
Sulphuric Acid ◽  
Sea Water ◽  
Picric Acid ◽  
Colorimetric Method ◽  
Ammonium Molybdate ◽  
Potassium Chromate ◽  
Distilled Water ◽  
Yellow Colour ◽  
Standard Solutions

Silicon in sea water may be present in suspension, in particles of clay or sand, as a constituent of diatoms, etc., or in solution. Some silicon in solution occurs in the form of silicate. This is usually estimated by the colorimetric method of Diénert & Wandenbulcke (1923), which makes use of the yellow colour of the silicomolybdic acid which is formed when ammonium molybdate and sulphuric acid are added to the water (Atkins, 1923). The colour may be compared with that of standard solutions of picric acid (Diénert & Wandenbulcke, 1923) or potassium chromate (Swank & Mellon, 1934). The method is simple but the colour in sea water is often faint and is not easy to match visually, nor is its intensity strictly proportional to the concentration of silicate. Less colour is produced in sea water than in standard solutions made with distilled water and this ‘salt error’ must be allowed for (Brujewicz & Blinov, 1933; Wattenberg, 1937; Robinson & Spoor, 1936).

A Colorimetric Method for Determination of Saxitoxin

1977 ◽  
Vol 34 (4) ◽  
pp. 559-563 ◽  
Author(s):  
R. M. Gershey ◽  
R. A. Nevé ◽  
D. L. Musgrave ◽  
P. B. Reichardt
Keyword(s):  
Hydrogen Peroxide ◽  
Key Words ◽  
Colorimetric Method ◽  
Peroxide Oxidation ◽  
Paralytic Shellfish Poison ◽  
Paralytic Shellfish ◽  
Hydrogen Peroxide Oxidation ◽  
Cleanup Procedure

Saxitoxin, the paralytic shellfish poison, can be conveniently determined by colorimetry after hydrogen peroxide oxidation and reaction of the resultant guanidine with biacetyl. This colorimetric method has been used to assay saxitoxin quantitatively at concentrations down to 0.5 μg/ml. Saxitoxin levels in the Alaskan butter clam (Saxidomus gigantea) have also been determined by this test coupled with a preliminary chromatographic cleanup procedure. Key words: saxitoxin, paralytic shellfish poison, colorimetry, Alaskan butter clam (Saxidomus gigantea)

Colorimetric Method for the Determination of Starch in Tobacco

1972 ◽  
Vol 55 (1) ◽  
pp. 209-213
Author(s):  
A J Sensabaugh ◽  
Kenneth L Rush
Keyword(s):  
Acid Treatment ◽  
Quantitative Measurement ◽  
Starch Content ◽  
Colorimetric Method ◽  
Colored Complex ◽  
Iodine Complex ◽  
Mesh Screen ◽  
Colorimetric Data ◽  
Tobacco Sample

Abstract A method is presented for extracting and determining the starch content of tobacco. Dried tobacco is ground to pass a 60 mesh screen, interfering colored materials are removed from the tobacco by a methanol-water extraction, and the starch is extracted from the tobacco by a perchloric acid treatment. Final quantitative measurement of the starch is made on a colored starch–iodine complex. The absorbance of this colored complex at 600 nm is proportional to the concentration of the starch in solution and can be related to the starch content of the original tobacco sample. Also included is a procedure for the isolation of pure tobacco starch. Such material was prepared for use as a calibration standard needed for the calculation of starch content of tobacco samples from the colorimetric data.

scholarly journals Determination of hydrogen peroxide on N95 masks after sanitization using a colorimetric method

MethodsX ◽  
2021 ◽  
pp. 101485
Author(s):  
Paramee Kumkrong ◽  
Ludmila Scoles ◽  
Yvan Brunet ◽  
Scott Baker
Keyword(s):  
Hydrogen Peroxide ◽  
Colorimetric Method

Colorimetric Determination of 2,6-Dichloro-4-Nitroaniline (Dichloran) Residues in Foods

1969 ◽  
Vol 52 (4) ◽  
pp. 797-799
Author(s):  
James A Heagy
Keyword(s):  
Colorimetric Method ◽  
Chromatographic Column ◽  
Colorimetric Determination ◽  
Modified Method ◽  
Color Development ◽  
Development Procedure

Abstract The colorimetric method of Groves and Chough for determining dichloran residues in food was modified. The modified method uses a Florisil chromatographic column cleanup and a Bratton-Marshall color development procedure. Recoveries ranged from 90 to 100% for eight crops. The method is recommended for further study.

scholarly journals DETERMINATION OF GLUCOSE OXIDASE ACTIVITY BY SPECTROPHOTOMETRIC METHOD

2021 ◽  
pp. 18-25
Author(s):  
Борис Борисович Тихонов ◽  
Полина Юрьевна Стадольникова ◽  
Александр Иванович Сидоров ◽  
Михаил Геннадьевич Сульман
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Oxidase Activity ◽  
Calibration Graph ◽  
Ammonium Molybdate ◽  
Initial Glucose Concentration ◽  
Reproducible Method ◽  
Hydrogen Peroxide Formation ◽  
Glucose Oxidation Reaction

В статье рассматривается универсальная, чувствительная, быстрая и воспроизводимая методика определения активности глюкозооксидазы, основанная на окислении пероксидом водорода йодида калия в присутствии молибдата аммония и фотометрировании образующегося синего комплекса «йод-крахмал». Построен калибровочный график для определения концентрации пероксида водорода в реакционной смеси. Проведен анализ образования пероксида водорода в реакции окисления глюкозы глюкозооксидазой при варьировании начальной концентрации глюкозы. The article developed a universal, sensitive, fast and reproducible method for determining glucose oxidase activity, based on the oxidation of potassium iodide by hydrogen peroxide in the presence of ammonium molybdate and photometry of the resulting blue iodine-starch complex. A calibration graph is constructed to determine the concentration of hydrogen peroxide in the reaction mixture. Analysis of hydrogen peroxide formation in glucose oxidation reaction with glucose oxidase at variation of initial glucose concentration was performed.

Optimization of Conditions for Accurate Phosphonate and Total Phosphorus Assay on Lipid Samples, in Conjunction with Thin-Layer Chromatography

1986 ◽  
Vol 41 (3) ◽  
pp. 263-268 ◽  
Author(s):  
Vassilios M. Kapoulas ◽  
George Th. Tsangaris
Keyword(s):  
Accurate Determination ◽  
Ammonium Molybdate ◽  
Inorganic Acid ◽  
Color Formation ◽  
Color Development ◽  
Phosphomolybdenum Blue ◽  
Layer Chromatography ◽  
Total P ◽  
Separate Calibration

The range of inorganic acid normalities for maximum color formation of the phosphomolybdenum- blue complex (under heating) increases by elevating the ammonium molybdate concentration, and at a ratio of molybdate molarity/acid normality equal to 10, there is maximum color development at any acid normality in the range 1-4 ɴ with either HClO4 or H2SO4 (or their mixtures). On the basis of these features a revised method is described for the accurate determination of phosphonate-P percent of total-P. on lipid extracts and on TLC bands. The color at the final step, in both cases, is developed under the same conditions of molybdate, HClO4 and H2SO4 concentrations, thus avoiding possible errors produced by the use of two separate calibration curves.

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