Semiautomated microfluorometric instrument and reagent system for monitoring disease-related immunosuppression.

1982 ◽  
Vol 28 (9) ◽  
pp. 1887-1893 ◽  
Author(s):  
D F Ranney ◽  
A J Quattrone
Keyword(s):  
Immune Response ◽  
Dna Content ◽  
Peripheral Blood ◽  
Fluorescence Enhancement ◽  
Photon Counting ◽  
Peripheral Blood Lymphocytes ◽  
Response Modulation ◽  
Blood Lymphocytes ◽  
Complex Test ◽  
Immune Response Modulation

Abstract Several common metabolites and drugs in the serum in of patients with inflammatory, infectious, autoimmune, immunodeficient, neoplastic, and toxicant-induced diseases can produce artifactual suppression of the [methyl-3H]-thymidine assay, which is widely used to evaluate lymphocyte responsiveness. We have developed a sensitive, semiautomated, fluorescence-enhancement assay in which true immunosuppressors are measured in the presence of absence of such interfering substances. Peripheral blood lymphocytes are activated with mitogens in standard microtiter culture trays. Changes in lymphocyte DNA content are quantified with a reagent formulation containing mithramycin, the fluorescence of which is enhanced on binding to DNA in the presence of MgCl2. We solubilize cells within the intact microtiter tray by using an automated, inverted "Array Sonicator," and measure fluorescence with an automated, photon-counting fluorometer. With this system, immune response modulation can be accurately assessed in the presence of patients' sera and other complex test substances (e.g., supernates from hybridomas, fermentation vats, viral preparations, and macrophage cultures.

Immune Response and Mitosis of Human Peripheral Blood Lymphocytes in vitro

Science ◽  
1963 ◽  
Vol 142 (3596) ◽  
pp. 1185-1187 ◽  
Author(s):  
K. Hirschhorn ◽  
F. Bach ◽  
R. L. Kolodny ◽  
I. L. Firschein ◽  
N. Hashem
Keyword(s):  
Immune Response ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

scholarly journals Human immune response to HIV-1 Nef. II. Induction of HIV-1/HIV-2 Nef cross-reactive cytotoxic T lymphocytes in peripheral blood lymphocytes of non-infected healthy individuals

1996 ◽  
Vol 8 (4) ◽  
pp. 577-584 ◽  
Author(s):  
Maria Lucchiari-Hartz ◽  
Monika Bauer ◽  
Gabriele Niedermann ◽  
Bernhard Maier ◽  
Andreas Meyerhans ◽  
...  
Keyword(s):  
Immune Response ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Healthy Individuals ◽  
Blood Lymphocytes ◽  
Human Immune Response ◽  
Human Immune ◽  
Hiv 1

Immune response and mitosis of human peripheral blood lymphocytes in vitro

1964 ◽  
Vol 2 (4) ◽  
pp. 570
Author(s):  
n Hirschhor ◽  
h Bac ◽  
R. L. Kolodny ◽  
I. L. Firschein ◽  
m Hashe
Keyword(s):  
Immune Response ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

Dynamics of ribosomal activity and protein production in peripheral blood lymphocytes during an immune response

BIOPHYSICS ◽  
2008 ◽  
Vol 53 (4) ◽  
pp. 296-298 ◽  
Author(s):  
N. A. Karnaukhova ◽  
L. A. Sergievich ◽  
V. N. Karnaukhov
Keyword(s):  
Immune Response ◽  
Peripheral Blood ◽  
Protein Production ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Ribosomal Activity

scholarly journals S. PYOGENES M49-16 ARGININE DEIMINASE INHIBITS PROLIFERATIVE ACTIVITY IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES

2019 ◽  
Author(s):  
E. Starickova ◽  
T. Leveshko ◽  
D. Churakina ◽  
I. Kudryavtsev ◽  
L. Burova ◽  
...  
Keyword(s):  
Immune Response ◽  
Cell Proliferation ◽  
Peripheral Blood ◽  
Parental Strain ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Arginine Deiminase ◽  
Intact Cells ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

Arginine deiminase is one of three enzymes constituting the arginine deiminase system in bacteria. It was demonstrated that arginine deiminase exerts anti-proliferative effects on some primary and immortalized mouse and human cells. It is assumed that the inhibitory effect of arginine deiminase on cell proliferation might be related to its ability to result in the arginine exhaustion. T lymphocytes depend on arginine for proliferation, T-cell receptor complex expression, and the differentiation of memory cells. The aim of the current study was to investigate an impact streptococcal arginine deiminase on functions of human peripheral blood lymphocytes. For this, we comparatively analyzed effects of Supernatant of Destroyed Streptococcal Cells (SDSCs) derived from parental strain S. pyogenes M49-16 and its isogenic mutant S. pyogenes M49-16delArcA bearing inactivated arginine deiminase gene (ArcA) on immune cell functions.An impact of supernatants on cell viability was estimated by staining with DAPI dye. Cell proliferation was assessed by MTT-test and flow cytometry by using the method based on intracellular protein staining with vital fluorescent CFSE (carboxyfluoresceinsuccinimidylester) dye. In addition, the level of lymphocyte tyrosine phosphatase CD45 expression in various culturing conditions was evaluated. It was demonstrated that S. pyogenes M49-16 SDSCs had no impact on cells viability. Parental strain-derived SDSC exerted virtually no effect on intact cells proliferation, but considerably suppressed ConA-induced cell proliferation.At the same time, mutant strain-derived SDSC significantly stimulated spontaneous cell proliferation, but not that one after mitogen exposure. It was observed that increased proliferation was accompanied by upregulated CD45 expression, although it was not significant in all cases. These data allow to conclude that bacterial arginine deiminase could be one of pathogenicity factors able to limit lymphocyte proliferation and immune response and could be a part of pathogen strategy to suppress immune response in order to improve bacterial growth and dissemination.

scholarly journals Phenotypic Study of Peripheral Blood Lymphocytes and Humoral Immune Response in Helicobacter pylori Infection According to Age

2005 ◽  
Vol 62 (1) ◽  
pp. 63-70 ◽  
Author(s):  
T. F. Soares ◽  
G. A. Rocha ◽  
A. M. C. Rocha ◽  
R. Correa-Oliveira ◽  
O. A. Martins-Filho ◽  
...  
Keyword(s):  
Immune Response ◽  
Helicobacter Pylori ◽  
Humoral Immune Response ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Helicobacter Pylori Infection ◽  
Blood Lymphocytes ◽  
Humoral Immune

scholarly journals Cell-mediated immune response to bacterial products in human tonsils and peripheral blood lymphocytes.

1979 ◽  
Vol 23 (2) ◽  
pp. 347-352 ◽  
Author(s):  
M M Drucker ◽  
Y Agatsuma ◽  
I Drucker ◽  
E Neter ◽  
J Bernstein ◽  
...  
Keyword(s):  
Immune Response ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Cell Mediated Immune Response ◽  
Bacterial Products

Normalization of immune response and phosphoinositide fatty acid composition of peripheral blood lymphocytes in an alcoholic patient after alcohol abstinence

IUBMB Life ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 359-367
Author(s):  
G. F. Stefanini ◽  
E. Castelli ◽  
F. G. Foschi ◽  
L. Marsigli ◽  
G. Addolorato ◽  
...  
Keyword(s):  
Immune Response ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Alcoholic Patient ◽  
Blood Lymphocytes ◽  
Alcohol Abstinence
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