Two direct radioimmunoassays for 17 beta-estradiol evaluated for use in monitoring in vitro fertilization.

1984 ◽  
Vol 30 (5) ◽  
pp. 787-790 ◽  
Author(s):  
R V Haning ◽  
S M Meier ◽  
L M Boehnlein ◽  
M Gerrity ◽  
S S Shapiro
Keyword(s):  
In Vitro Fertilization ◽  
Solid Phase ◽  
Correlation Coefficients ◽  
Comparison Method ◽  
Ovarian Activity ◽  
Vitro Fertilization ◽  
Serum Pool ◽  
Clinically Significant ◽  
Ultrasound Parameters

Abstract Two direct 125I radioimmunoassays for 17 beta-estradiol were evaluated by comparing results with those obtained by a comparison RIA involving extraction and chromatography (x) and with ultrasound parameters of ovarian activity. The correlation coefficients (R2) for the Immuchem Covalent-Coat Solid Phase 125I results (y1) and the Pantex Immuno-direct results (y2) with the comparison method results were 0.56 and 0.96, respectively, after log transformation of data (N = 42). Similar differences were observed in the correlations with the ultrasound parameters. We also evaluated the untransformed data by linear regression and obtained: y1 = 254 + 0. 410x (R2 = 0.65) and y2 = 48.2 + 0. 749x (R2 = 0.96). Between- and within-assay variations for a serum pool containing 17 beta-estradiol at 1278 pg/mL were respectively 9.0% and 9.4% for Immuchem , 3.3% and 6.7% for Pantex , and 7.3% and 9.4% for the comparison method; for the 267 pg/mL pool, these were 21.8% and 16.6% for Immuchem , 4.7% and 4.6% for Pantex , and 21.2% and 13.1% for the comparison method. Differences in the results obtained with the Pantex method and the comparison method were not clinically significant for monitoring superovulation for in vitro fertilization; the performance of the Immuchem method was less satisfactory.

scholarly journals Impact of COVID-19 Pandemic on COMPI Fertility Problem Stress Scale and the State-Trait Anxiety Scale in Patients Undergoing IVF/ICSI

2021 ◽  
pp. 1-7
Author(s):  
Arzu Yurci ◽  
Saban Karayagiz
Keyword(s):  
In Vitro Fertilization ◽  
Trait Anxiety ◽  
Correlation Coefficients ◽  
Anxiety Scale ◽  
Lower State ◽  
Cross Sectional ◽  
Vitro Fertilization ◽  
Stress Scale ◽  
Fertility Problem

OBJECTIVE: This study aimed to investigate the effect of the COVID-19 outbreak on infertile couples dealing with stress and anxiety during in vitro fertilization treatment. STUDY DESIGN: 252 infertile patients who applied for in vitro fertilization treatment were included in this cross-sectional study. Data were collected via four data collection tools including socio-demographic form, COVID-19 Inventory (COVID-I), COMPI fertility problem stress scale (COMPI-FPSS), and state-trait anxiety scale. Data analysis was conducted by SPSS statistical software included statistical analysis such as averages, standard deviations, correlation, regression, and t-test. RESULTS: Both negative and weak correlations were found between COMPI fertility problem stress scale and subscales of state-trait anxiety scale as well as the total score of state-trait anxiety scale. In terms of socio-demographic characteristics of the participants and their responses in state-trait anxiety scale and ISS forms, the correlation coefficients were also so low. The results also showed that public officials, health professionals, and educators showed lower state-trait anxiety scale (stress-related anxiety) scores. CONCLUSION: During the COVID-19 pandemic, there was an increase in spontaneous pregnancy expectations. Although participants were mostly stressed because of the COVID-19 outbreak, they didn’t change their in vitro fertilization treatment plans during the outbreak.

Hormones of oestrus and ovulation and their manipulation in marsupials

1996 ◽  
Vol 8 (4) ◽  
pp. 661 ◽  
Author(s):  
LA Hinds ◽  
TP Fletcher ◽  
JC Rodger
Keyword(s):  
In Vitro Fertilization ◽  
Tammar Wallaby ◽  
Monodelphis Domestica ◽  
Ovarian Activity ◽  
Vitro Fertilization ◽  
Macropus Eugenii ◽  
Australian Species ◽  
Maturation In Vitro ◽  
American Laboratory

Oestrus and ovulation occur spontaneously in the majority of marsupials, with behavioural oestrus usually occurring 1-2 days before ovulation. The hormone changes that occur at this time have been described in the most detail for the monovular tammar wallaby Macropus eugenii. The respective roles of the Graafian follicle, corpus luteum and the pituitary in the events leading up to oestrus and ovulation in this species are also reviewed. Recently, various protocols have been developed for superovulation of marsupials, including Australian species, such as the brush-tailed possum, fat-tailed dunnart, brush-tailed bettong and tammar wallaby, and the American laboratory opossum, Monodelphis domestica. These protocols provide an opportunity for studying the regulation of ovarian activity and for the collection of larger quantities of material for the study of gamete maturation, in vitro fertilization and embryonic development.

Characteristic and Pregnancy Rate of IVF Patient: a Retrospective Analysis from Two Centres

KnE Medicine ◽  
2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Budi Wiweko
Keyword(s):  
In Vitro Fertilization ◽  
Success Rate ◽  
Pregnancy Rate ◽  
Paternal Age ◽  
Medical Attention ◽  
Vitro Fertilization ◽  
Slow Development ◽  
Infertile Couples ◽  
Clinically Significant

<p>Introduction: According to recent statistics, infertility in Indonesia reached 21,9%. In-vitro fertilization (IVF) was a reliable treatment option for subfertility couples with success rate of 22-40%. Data from the Indonesia Association for In-Vitro Fertilization (Perfitri) in 2010 showed the country had only 2000 cases, one third of Vietnam’s and half of Thailand’s. The technology’s high cost has been cited as the cause behind the slow development of IVF in Indonesia.<br />Objective: We aim to evaluate the characteristics of patients and their pregnancy rate following in vitro fertilization in infertile couples from two different centres, Yasmin Clinic and Kedoya Clinic.<br />Material and methods: Cohort retrospective was used to analyse outpatient data from two fertility centres. A total of 510 outpatients’ data, 405 in Yasmin Clinic and 105 in Kedoya Clinic, between October 2014 to September 2015 was collected. Patients and in vitro fertilization cycle characteristics from both clinics were evaluated. The pregnancy rate after IVF was compared between the groups. <br />Result: Although maternal age (p=0,032) and paternal age (p=0,017) were statistically significant, they were not clinically significant. There were also no significant differences in the characteristics of in vitro fertilization in both clinics, clinically. However, pregnancy rate was higher in Yasmin Clinic than in Kedoya Clinic, with pregnancy rate of 64,8% and 45,7%, respectively (OR = 2,185; 95%CI 1,420-3,361; p=0,001).<br />Discussion: There were no differences between characteristics of patients and in vitro fertilization cycle in both centres. Nevertheless, pregnancy rate between Yasmin Clinic and Kedoya Clinic were statistically different, suggesting another factors that influence success rate of in vitro fertilization. This research showed that most patients seek medical attention at the age of 35, after 7 years of infertility, suggesting awareness of infertility problem is still low.</p>

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