Improved detection of oligoclonal IgG in cerebrospinal fluid by isoelectric focusing in agarose, double-antibody peroxidase labeling, and avidin-biotin amplification.

1984 ◽  
Vol 30 (7) ◽  
pp. 1246-1249 ◽  
Author(s):  
T Olsson ◽  
V Kostulas ◽  
H Link
Keyword(s):  
Cerebrospinal Fluid ◽  
Isoelectric Focusing ◽  
Present Method ◽  
Tension Headache ◽  
Cellulose Nitrate ◽  
Double Antibody ◽  
Coomassie Blue ◽  
The Central Nervous System ◽  
Oligoclonal Igg ◽  
Oligoclonal Igg Bands

Abstract To demonstrate oligoclonal IgG bands (I) in unconcentrated cerebrospinal fluid, we used isoelectric focusing in agarose followed by protein transfer to cellulose nitrate membrane, double-antibody peroxidase labeling, and avidin-biotin amplification. I can be reliably seen after isoelectric focusing of 5-microL specimens containing 125 ng of IgG (25 mg/L). Thus the technique is more sensitive than others (e.g., silver staining) and more reliable than radioimmunofixation. When we used this technique with fluids from 62 patients with multiple sclerosis and infectious disease of the central nervous system, 84% displayed I, a percentage not increased when the same specimens were concentrated to 3.5 g of IgG per liter, examined by agarose isoelectric focusing, and stained with Coomassie Blue. Results for 53 patients with tension headache and psychoneurosis were all negative. By obviating the need to concentrate samples of cerebrospinal fluid the present method is a useful, sensitive alternative for demonstrating I.

Isoelectric Focusing in an Immobilized pH Gradient for the Detection of Intrathecal IgG in Cerebrospinal Fluid: Sensitivity and Specificity for the Diagnosis of Multiple Sclerosis

1993 ◽  
Vol 30 (5) ◽  
pp. 463-468 ◽  
Author(s):  
G N Cowdrey ◽  
P J Tasker ◽  
B J Gould ◽  
M Rice-Oxley ◽  
G B Firth
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Sensitivity And Specificity ◽  
Isoelectric Focusing ◽  
High Specificity ◽  
Ph Gradient ◽  
Alkaline Ph ◽  
Oligoclonal Igg ◽  
Very High ◽  
Oligoclonal Igg Bands

Cerebrospinal fluid and serum from 192 patients was analysed for the presence of intrathecally synthesized oligoclonal IgG bands using isoelectric focusing in an immobilized pH gradient pH7–10. The sensitivity of this method for the diagnosis of multiple sclerosis (MS) was 95% (21 of 22), or 75% if patients with suspected MS were included. The specificity for the diagnosis of MS was 98%, or 96% if the suspected MS patients were included. The very high specificity may be because the intrathecally synthesized oligoclonal IgG associated with MS is more alkaline than IgG from serum and is better detected in an immobilized alkaline pH gradient.

Oligoclonal IgG bands in cerebrospinal fluid in various neurological diseases

1983 ◽  
Vol 13 (4) ◽  
pp. 434-439 ◽  
Author(s):  
Anita B. Chu ◽  
John L. Sever ◽  
David L. Madden ◽  
Marti Iivanainen ◽  
Marta Leon ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Neurological Diseases ◽  
Oligoclonal Igg ◽  
Oligoclonal Igg Bands

scholarly journals Oligoclonal IgG bands in the cerebrospinal fluid of portuguese patients with multiple sclerosis: negative results indicate benign disease

2005 ◽  
Vol 63 (2b) ◽  
pp. 375-379 ◽  
Author(s):  
Maria José Sá ◽  
Lucinda Sequeira ◽  
Maria Edite Rio ◽  
Edward J. Thompson
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Benign Disease ◽  
Serum Samples ◽  
Negative Results ◽  
Significant Difference ◽  
Relapsing Remitting ◽  
The Mean ◽  
Oligoclonal Igg ◽  
Oligoclonal Igg Bands

We assessed the frequency of cerebrospinal fluid (CSF) restricted oligoclonal IgG bands (IgG-OCB) in Portuguese multiple sclerosis (MS) patients and its relationship with outcome. Paired CSF/serum samples of 406 patients with neurological disorders were submitted to isoelectric focusing with immunodetection of IgG. Ninety-two patients had definite MS; non-MS cases were assembled in groups inflammatory/infectious diseases (ID, n=141) and other/controls (OD, n=173). We found in the MS group: mean duration, 38.9 months; clinically isolated syndromes, 24%; relapsing/remitting course (RR), 65%; in RR patients the mean EDSS was 2.1 and the mean index of progression was 0.31. Positive patterns significantly predominated in MS (82.6%; ID, 40.4%; OD, 3.5%). The sensitivity and the specificity of positive IgG-OCB for MS diagnosis was 82.6% and 79.9%, respectively. The sole statistically significant difference in the MS group was the lower progression index observed in negative cases. We conclude that the frequency of positive IgG-OCB patterns in our MS patients fits most values reported in the literature, and that negative results indicate benign disease.

Multiple sclerosis immunopathic trait and HLA-DR(2)15 as independent risk factors in multiple sclerosis

2007 ◽  
Vol 13 (4) ◽  
pp. 441-445 ◽  
Author(s):  
M. Callander ◽  
S. Haghighi ◽  
A.-M. Landtblom ◽  
C.E. Ahlgren ◽  
S.I. Nilsson ◽  
...  
Keyword(s):  
Risk Factors ◽  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Susceptibility Gene ◽  
Hla Dr ◽  
Healthy Siblings ◽  
Independent Risk Factors ◽  
Oligoclonal Igg ◽  
Hla Haplotypes ◽  
Oligoclonal Igg Bands

We analysed HLA haplotypes in pairs of 78 sporadic multiple sclerosis (MS) patients and 78 healthy siblings. The presence of 2 oligoclonal IgG bands, detected by immunoblotting of the cerebrospinal fluid in healthy siblings, has previously been defined as MS immunopathic trait (MSIT), based on a cut-off derived from healthy unrelated volunteers. The frequency of MSIT was 17.9% (n=14/78 siblings). The HLA-DR(15)2 allelle was present in 21.4% (n=3/14) of the siblings with MSIT, in 40.6% (n =26/64) of the siblings without MSIT, and in 59% (n =46/78) of the patients with clinically-definite (CD) MS. The distribution of zero, one or two HLA-DR(2)15 alleles was significantly skewed towards a lower allelle count in the siblings with MSIT compared with the group of unrelated siblings with MS (P=0.002), and also lower than their related siblings with MS (P=0.1). These results suggest that the MS susceptibility gene, HLA-DR(2)15 type, does not induce MSIT, and conceivably these are two separate risk factors in the development of MS. The effect of HLA-DR(2)15 and MSIT in sporadic MS appears to be synergistic. Multiple Sclerosis 2007; 13: 441-445. http://msj.sagepub.com

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