Oligoclonal IgG bands in Japanese patients with multiple sclerosis. A comparative study between isoelectric focusing with IgG immunofixation and high-resolution agarose gel electrophoresis

2005 ◽  
Vol 159 (1-2) ◽  
pp. 133-136 ◽  
Author(s):  
Ichiro Nakashima ◽  
Kazuo Fujihara ◽  
Shigeru Sato ◽  
Yasuto Itoyama
Keyword(s):  
Multiple Sclerosis ◽  
High Resolution ◽  
Comparative Study ◽  
Isoelectric Focusing ◽  
Gel Electrophoresis ◽  
Japanese Patients ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Oligoclonal Igg ◽  
Oligoclonal Igg Bands

scholarly journals Detection of Oligoclonal Immunoglobulins in Cerebrospinal Fluid by an Immunofixation-Peroxidase Method

2002 ◽  
Vol 48 (1) ◽  
pp. 167-173 ◽  
Author(s):  
Sophie Richard ◽  
Véronique Miossec ◽  
Jean-François Moreau ◽  
Jean-Luc Taupin
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
High Resolution ◽  
Gel Electrophoresis ◽  
Attractive Alternative ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Sample Concentration ◽  
Immunofixation Electrophoresis ◽  
Oligoclonal Igg

Abstract Background: The detection of intrathecal synthesis of immunoglobulins is used in the diagnosis of multiple sclerosis (MS). We tested the semiautomated immunofixation-peroxidase (IFPOD) technique, which uses high-resolution agarose gel electrophoresis (HRAGE) directly followed by immunofixation with a peroxidase-labeled anti-IgG antiserum to detect oligoclonal immunoglobulins in cerebrospinal fluid (CSF). Methods: We analyzed 230 consecutive matched serum/CSF pairs that arrived in the laboratory over a 6-month period with both IFPOD and our routine techniques, immunofixation electrophoresis (IFE) and HRAGE. For IFPOD, CSF samples were not concentrated before testing. Results: Among the 230 samples were 12 clinically definite MS, 33 clinically probable, and 20 clinically possible MS samples. IFPOD and HRAGE + IFE each detected oligoclonal IgG in CSF in 10, 16, and 7 cases of these respective groups. For clinically definite MS, sensitivity and specificity (95% confidence intervals) were, respectively, 83% (51–97%) and 79% (73–84%). Conclusions: The IFPOD technique performs comparably to other analytical methods, without the requirement for sample concentration, and may represent an attractive alternative in testing for intrathecal immunoglobulin synthesis.

Precision and Reliability of Paraprotein Determinations by High-Resolution Agarose Gel Electrophoresis

1989 ◽  
Vol 91 (4) ◽  
pp. 435-440 ◽  
Author(s):  
David Stemerman ◽  
Christine Papadea ◽  
David Martino-Saltzman ◽  
A. Christine O’connell ◽  
Barbara Demaline ◽  
...  
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis

scholarly journals Serum monoclonal immunoglobulin bands in undifferentiated lymphomas of Burkitt and non-Burkitt types

Blood ◽  
1983 ◽  
Vol 61 (4) ◽  
pp. 726-731
Author(s):  
I Magrath ◽  
D Benjamin ◽  
N Papadopoulos
Keyword(s):  
High Resolution ◽  
Tumor Markers ◽  
Light Chain ◽  
Gel Electrophoresis ◽  
Lymphoblastic Lymphoma ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Monoclonal Immunoglobulin ◽  
Potential Value

Using an improved electroimmunofixation technique that combines the sensitivity of high resolution agarose gel electrophoresis with the specificity of immunoprecipitation, we have demonstrated monoclonal immunoglobulin bands in the serum of patients with undifferentiated lymphomas of Burkitt and non-Burkitt types. Monoclonal bands were detected in the serum of 12 of 21 patients with extensive tumor, and 1 of 10 patients with minimal tumor. All of the bands were identified as IgM of a single light chain class. Such bands were not detected in the serum of patients with lymphoblastic lymphoma (7) or African Burkitt's lymphoma (6). There was disappearance of the bands after therapy and reappearance at relapse. These findings, coupled with previously reported in vitro information, indicate that undifferentiated lymphoma cells secrete immunoglobulin of IgM isotype. Therefore, such monoclonal bands may be of potential value as tumor markers.

Silver staining of unconcentrated cerebrospinal fluid in agarose gel (Panagel) electrophoresis.

1984 ◽  
Vol 30 (5) ◽  
pp. 735-736 ◽  
Author(s):  
P D Mehta ◽  
S P Mehta ◽  
B A Patrick
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Silver Staining ◽  
Clinical Laboratory ◽  
Neurological Diseases ◽  
Agarose Gel ◽  
Oligoclonal Igg ◽  
Multiple Sclerosis Patients ◽  
Coomassie Brilliant ◽  
Oligoclonal Igg Bands

Abstract We subjected cerebrospinal fluid (CSF) from 20 patients with multiple sclerosis and 20 patients with other neurological diseases to agarose gel ( Panagel ) electrophoresis followed by staining with silver. Ten microliters of unconcentrated CSF from multiple sclerosis patients containing 0.4 to 0.8 microgram of immunoglobulin G was found to be optimum for detection of oligoclonal IgG bands, so identified by immunofixation. The band patterns for unconcentrated CSF stained with silver were almost identical to those for the same CSF concentrated 40-fold and stained with Coomassie Brilliant Blue. Silver staining thus enables the clinical laboratory to electrophorese unconcentrated CSF on commercially prepared ( Panagel ) plates.

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