Determination of amylase activity in serum by using a wheat germ inhibitor with the Du Pont aca.
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Abstract A rapid procedure for determining salivary- and pancreatic-type amylase (EC 3.2.1.1) in serum by incorporating a wheat germ inhibitor (from Triticum aestivum) was developed for the Du Pont aca IV analyzer. Under optimal assay conditions, activities of salivary and pancreatic amylase were inhibited by 93% and 19%, respectively. The 95% central reference interval for the percentage of inhibition of serum amylase was 38-84%. Patients with acute pancreatitis showed less than 26% inhibition of amylase after addition of the wheat germ extract, reflecting the prevalence of pancreatic-type amylase in this disorder.
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1993 ◽
Vol 39
(12)
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pp. 2495-2499
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1963 ◽
Vol 41
(1)
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pp. 2107-2121
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Keyword(s):
1963 ◽
Vol 18
(1)
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pp. 77-82
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