Albumin Cooperstown: a serum albumin variant with the same (313 Lys----Asn) mutation found in albumins in Italy and New Zealand.

1988 ◽  
Vol 34 (1) ◽  
pp. 183-187 ◽  
Author(s):  
K Huss ◽  
F W Putnam ◽  
N Takahashi ◽  
Y Takahashi ◽  
G A Weaver ◽  
...  
Keyword(s):  
New York ◽  
Liquid Chromatography ◽  
New Zealand ◽  
High Performance ◽  
Genetic Variant ◽  
New York State ◽  
Single Nucleotide ◽  
Albumin Variant ◽  
Albumin Molecule ◽  
Normal Albumin

Abstract Albumin Cooperstown is an electrophoretically fast genetic variant (alloalbumin) inherited in a family in New York State. To determine the structural change, we separated the alloalbumin from normal albumin A by "high-performance" liquid chromatography, reduced and carboxymethylated it, and cleaved it with CNBr. Isoelectric focusing showed that the substitution was in fragment CB4 (residues 299 to 329). The variant CNBr fragment from the albumin of two siblings was purified by liquid chromatography. Automated sequence analysis established the substitution of lysine-313 by asparagine (313 Lys----Asn), which corresponds to mutation of a single nucleotide base. The same substitution has recently been reported in albumin variants in Italy and New Zealand. This provides the first evidence for independent mutations at a single site within the structure of the mature albumin molecule. Because of the significance of albumin for study of protein evolution, clinical chemists are asked to be on the alert for cases of bisalbuminemia.

In-Service Performance of High-Performance Concrete Bridge Decks

2000 ◽  
Vol 1696 (1) ◽  
pp. 193-196 ◽  
Author(s):  
Sreenivas Alampalli ◽  
Frank Owens
Keyword(s):  
New York ◽  
High Performance ◽  
Recent Literature ◽  
High Performance Concrete ◽  
Bridge Decks ◽  
New York State ◽  
Crack Density ◽  
Transverse Cracking ◽  
Concrete Bridge Decks ◽  
Better Than

The current statewide standard for New York State bridge decks is Class HP (high-performance) concrete. This mix was introduced in April 1996 to increase deck durability by reducing cracking and permeability. Since its introduction, more than 80 bridge decks have been built with Class HP concrete. To compare the performance of Class HP concrete with that of previously specified concrete, the decks were visually inspected. Results indicated that Class HP decks performed better than previously specified concrete in resisting both longitudinal and transverse cracking. Furthermore, of the 84 decks inspected, 49 percent exhibited no cracking at all, but of those that had cracked, 88 percent exhibited equal or less longitudinal cracking and 80 percent exhibited equal or less transverse cracking than previously specified concrete. A final result showed that average transverse crack density on Class HP decks, excluding uncracked decks, was 6.9 cm/m2. This value is comparable with crack densities for other decks (not using HP mix) that were reported in recent literature.

Application of denaturing high-performance liquid chromatography for mapping of single nucleotide polymorphisms in barley (Hordeum vulgare L.)

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 523-528 ◽  
Author(s):  
Raja Kota ◽  
Markus Wolf ◽  
Wolfgang Michalek ◽  
Andreas Graner
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Hordeum Vulgare ◽  
Dna Sequence ◽  
High Throughput ◽  
Doubled Haploid ◽  
High Performance ◽  
Nucleotide Polymorphisms ◽  
Hordeum Vulgare L ◽  
Single Nucleotide

Recent advances in DNA sequence analysis and the establishment of high-throughput assays have provided the framework for large-scale discovery and analysis of DNA sequence variation. In this context, single nucleotide polymorphisms (SNPs) are of particular interest. To initiate a systematic approach to develop an SNP map of barley (Hordeum vulgare L.), we have employed denaturing high-performance liquid chromatography (DHPLC) to analyse segregating SNP patterns in a doubled-haploid (DH) mapping population. To this end, SNPs between the parental genotypes were identified using a direct sequencing approach. Once a SNP was established between the parents, the optimal melting temperature of the PCR fragment containing the SNP was predicted for its analysis by DHPLC. Following the detection of the optimal temperature, the DH lines were analysed for the presence of either of the alleles. To test the utility of the analysis, data from previously mapped RFLP markers from which these SNPs were derived were compared. Results from these experiments indicate that DHPLC can be efficiently employed in analysing SNPs on a high-throughput scale.Key words: denaturing high performance liquid chromatography, doubled-haploid lines, restriction fragment length polymorphism, genetic mapping, molecular markers.

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